Article
Biotechnology & Applied Microbiology
Nathalie N. S. E. Henriksen, Mads Frederik Hansen, Heiko T. Kiesewalter, Jakob Russel, Joseph Nesme, Kevin R. Foster, Birte Svensson, Gunnar Oregaard, Jakob Herschend, Mette Burmolle
Summary: This study shows that cultivation in a structured environment facilitates coexistence, evolution, and adaptation of bacterial communities. Co-cultivation in a biofilm model resulted in stable coexistence of two bacterial species and drove the evolution of high-yield variants. This study emphasizes the importance of structured environments for the cultivation and evolution of industrially important bacterial communities.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Pharmacology & Pharmacy
Giovanna A. Belo, Barbara F. Cordeiro, Emiliano R. Oliveira, Marina P. Braga, Sara H. da Silva, Bruno G. Costa, Flaviano dos S. Martins, Gwenael Jan, Yves Le Loir, Alfonso Gala-Garcia, Enio Ferreira, Vasco Azevedo, Fillipe L. R. do Carmo
Summary: The study showed that the SlpB protein can enhance the probiotic potential of L. lactis strain to alleviate DSS-induced colitis in mice.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Pui Yan Siak, Kuan Yee Wong, Adelene Ai-Lian Song, Raha Abdul Rahim, Lionel Lian Aun In
Summary: A modified G12A-K-ras epitope was developed as a potential vaccine against KRAS cancers, utilizing L. lactis as a delivery vehicle and enhancing antigenicity. Experimental results indicated that the 139A-TTD vaccine successfully stimulated an antigen-specific immune response, with TTD fusion successfully enhancing immune responses.
Article
Biotechnology & Applied Microbiology
Hoda Shirdast, Fatemeh Ebrahimzadeh, Amir Hossein Taromchi, Yousef Mortazavi, Abdolreza Esmaeilzadeh, Mohammad Hadi Sekhavati, Keivan Nedaei, Esmat Mirabzadeh
Summary: Immunization using recombinant L. lactis expressing Omp31 induces both humoral and cellular immune responses in mice, showing the promising potential as an intervention against Brucella melitensis infections.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Biotechnology & Applied Microbiology
Yue Chen, Lei Qiao, Xiaofan Song, Li Ma, Xina Dou, Chunlan Xu
Summary: This study demonstrated that L. lactis NZ9000-SeNPs effectively alleviated ETEC K88-induced intestinal barrier dysfunction in C57BL/6 mice by increasing villus height, reducing inflammatory cytokines, enhancing antioxidant enzyme activities, and modulating tight junction protein expression levels. These results suggest that L. lactis NZ9000-SeNPs could serve as a promising and safe selenium supplement for food or feed additives.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sakineh Yeganeh, Milad Adel, Ahmad Nosratimovafagh, Mahmoud A. O. Dawood
Summary: The study investigated the potential probiotic effect of Lactococcus lactis subsp. lactis strain PTCC 1403 on rainbow trout in terms of growth, hematobiochemical, immune responses, and resistance to Yersinia ruckeri infection. The results showed beneficial effects on feed efficiency, immune response, and resistance to infection.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Agriculture, Multidisciplinary
Bibek Byanju, Swastik Sen, Thomas Mansell, Buddhi P. P. Lamsal
Summary: Corn steep liquor (CSL) and thin stillage were evaluated as growth media for recombinant Lactococcus lactis to produce antifreeze proteins (AFPs). Light CSL and thin stillage were optimized with additives and trace elements to enhance the growth of wild-type and recombinant strains. The fermentation supernatants showed longer times to supercool and freeze, indicating potential use as antifreeze compounds in frozen food and non-food applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Keila Lopes Mendes, Deborah de Farias Lelis, Daniela Fernanda de Freitas, Luiz Henrique da Silveira, Alfredo Mauricio Batista de Paula, Andre Luiz Sena Guimaraes, Janaina Ribeiro Oliveira, Marileia Chaves Andrade, Sergio Avelino Mota Nobre, Sergio Henrique Sousa Santos
Summary: The study demonstrated that the combination of resveratrol and Lactococcus lactis can reduce body weight, improve lipid metabolism, and decrease the expression of inflammatory markers, showing potential in preventing the development of liver diseases.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Immunology
Donya Kazemi, Abbas Doosti, Mostafa Shakhsi-Niaei
Summary: This study developed an oral vaccine based on recombinant probiotics for the prevention of Brucella infection. The results showed that the vaccine had good immunogenicity and significantly improved the immune response and cellular immunity in mice. In addition, the inflammatory response and tissue damage were also reduced in the vaccine group.
MICROBIAL PATHOGENESIS
(2023)
Article
Chemistry, Applied
Min Li, Weicheng Li, Dongyu Li, Juanjuan Tian, Luyao Xiao, Lai-Yu Kwok, Wei Li, Zhihong Sun
Summary: Exopolysaccharides (EPSs) from Lactococcus lactis subsp. lactis IMAU11823 were found to have strong reducing power and no cytotoxicity. The EPSs had different sugar compositions and molecular weights, with EPS-1 being a linear structure composed of glucose and mannose, and EPS-2 being a more complex, non-linear structure composed of mannose, glucose, and rhamnose. Furthermore, a biosynthesis model for EPS production in the IMAU11823 strain was proposed. This study broadens the understanding of the formation, structure, and function of complex EPSs produced by Lactococcus lactis subsp. lactis IMAU11823.
Article
Food Science & Technology
Xin Shen, Weicheng Li, Hongyu Cai, Shuai Guo, Min Li, Yangshuo Liu, Zhihong Sun
Summary: This study analyzed the fermentation capacity and metabolic profiles of 17 L. lactis subsp. lactis strains and found significant metabolic differences between strains with different fermentation rates. Peptides, esters, and tributyrin were identified as biomarkers to distinguish between fast and slow fermentation groups.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Miyu Taniguchi, Takashi Kuda, Moemi Takei, Hajime Takahashi, Bon Kimura
Summary: The freshwater cyanobacterium Aphanizomenon flos-aquae, harvested from Upper Klamath Lake in Oregon, USA, is used as a functional food material and can be fermented with Lactobacillus plantarum and Lactococcus lactis subsp. lactis. Feeding fermented Aphanizomenon flos-aquae (F-AFA) to mice resulted in lower body weight gains and higher caecal microbiome alpha diversity compared to feeding non-fermented A. flos-aquae. The F-AFA group showed higher abundances of certain bacteria belonging to the phylum Bacteroidetes and Firmicutes.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Oncology
Katarzyna Kaczmarek, Jerzy Wieckiewicz, Kazimierz Weglarczyk, Maciej Siedlar, Jarek Baran
Summary: This study investigated the use of genetically modified Lactococcus lactis bacteria for local delivery of human soluble TRAIL in CRC treatment. The results showed selective elimination of CRC cells and enhanced anti-tumor activity with oral administration of Metformin.
Article
Microbiology
Camila Prosperi de Castro, Bianca Mendes Souza, Pamela Mancha-Agresti, Vanessa Bastos Pereira, Meritxell Zurita-Turk, Tatiane Melo Preisser, Vanessa Pecini da Cunha, Janete Soares Coelho dos Santos, Sophie Yvette Leclercq, Vasco Azevedo, Anderson Miyoshi
Summary: The development of a new tuberculosis vaccine strategy utilizing a lactococcal DNA vaccine fusion of ESAT-6 and Ag85A has shown promising results in mouse immunization studies, inducing significant cellular and humoral immune responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Valerie Laroute, Nathalie Aubry, Marjorie Audonnet, Muriel Mercier-Bonin, Marie-Line Daveran-Mingot, Muriel Cocaign-Bousquet
Summary: This study combined genotypic and phenotypic approaches to investigate the diversity of GABA production in lactococci. Through comparative genomic analysis and phenotypic analysis, it was found that GABA production varied widely among different strains and species, and the regulation of GAD gene expression was identified as the major contributor to this diversity.
MICROBIAL CELL FACTORIES
(2023)
Article
Food Science & Technology
Lena Fritsch, Arnaud Felten, Federica Palma, Jean-Francois Mariet, Nicolas Radomski, Michel-Yves Mistou, Jean-Christophe Augustin, Laurent Guillier
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Meryl Vila Nova, Kevin Durimel, Kevin La, Arnaud Felten, Philippe Bessieres, Michel-Yves Mistou, Mahendra Mariadassou, Nicolas Radomski
Article
Microbiology
Nicolas Radomski, Sabrina Cadel-Six, Emeline Cherchame, Arnaud Felten, Pauline Barbet, Federica Palma, Ludovic Mallet, Simon Le Hello, Francois-Xavier Weill, Laurent Guillier, Michel-Yves Mistou
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Federica Palma, Thomas Brauge, Nicolas Radomski, Ludovic Mallet, Arnaud Felten, Michel-Yves Mistou, Anne Brisabois, Laurent Guillier, Graziella Midelet-Bourdin
Article
Microbiology
Pierre-Emmanuel Douarre, Ludovic Mallet, Nicolas Radomski, Arnaud Felten, Michel-Yves Mistou
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Yann Sevellec, Sophie A. Granier, Simon Le Hello, Francois-Xavier Weill, Laurent Guillier, Michel-Yves Mistou, Sabrina Cadel-Six
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Deborah Merda, Arnaud Felten, Noemie Vingadassalon, Sarah Denayer, Yacine Titouche, Lucia Decastelli, Bernadette Hickey, Christos Kourtis, Hristo Daskalov, Michel-Yves Mistou, Jacques-Antoine Hennekinne
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
L. Schwendimann, D. Merda, T. Berger, S. Denayer, C. Feraudet Tarisse, A. J. Klaui, S. Messio, M. Y. Mistou, Y. Nia, J. A. Hennekinne, H. U. Graber
Summary: This study investigated the relationship between the vSa beta type, clonal complex (CC), and the production of enterotoxins SEG and SEI by Staphylococcus aureus strains. It was found that the amount of SEG and SEI produced correlated with the vSa beta type and CC of a strain, and the whole-genome sequencing data could be used to predict enterotoxin production and source of an outbreak strain. These results show promising indications for better understanding the role SEG and SEI play in foodborne outbreaks caused by S. aureus.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mathilde Bonis, Arnaud Felten, Sylvie Pairaud, Angelie Dijoux, Veronique Maladen, Ludovic Mallet, Nicolas Radomski, Arnaud Duboisset, Chantal Arar, Xavier Sarda, Gaelle Vial, Michel-Yves Mistou, Olivier Firmesse, Jacques-Antoine Hennekinne, Sabine Herbin
Summary: This study revealed that Bacillus thuringiensis (Bt) could be a major causative agent of foodborne outbreaks (FBOs), with most FBO-associated Bt isolates showing a genetic relationship. More than 51% of Bt isolates were found in dishes containing raw vegetables, particularly tomatoes.
Article
Microbiology
Sabrina Cadel-Six, Emeline Cherchame, Pierre-Emmanuel Douarre, Yue Tang, Arnaud Felten, Pauline Barbet, Eva Litrup, Sangeeta Banerji, Sandra Simon, Federique Pasquali, Michele Gourmelon, Nana Mensah, Maria Borowiak, Michel-Yves Mistou, Liljana Petrovska
Summary: The study highlights the evolution of the epidemic European monophasic variant of Salmonella enterica serovar Typhimurium (S. 1,4,[5],12:i:-) ST34, which occurred in two steps in the 1980s and 1990s, involving the acquisition of multiple heavy metal resistances to adapt to the environment and enhance antimicrobial resistance. This evolution was facilitated by the integration of different genetic elements and phages, contributing to the spread and success of this serovar.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Michael Koczerka, Pierre-Emmanuel Douarre, Florent Kempf, Sebastien Holbert, Michel-Yves Mistou, Olivier Grepinet, Isabelle Virlogeux-Payant
Summary: The study revealed the widespread presence of the rck gene in various subspecies of Salmonella, raising questions about its evolution. The results emphasize the important role of rck in virulence and contribute to understanding the role of Rck in the pathogenesis of Salmonella.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Madeleine De Sousa Violante, Valerie Michel, Karol Romero, Laetitia Bonifait, Louise Bauge, Agnes Perrin-Guyomard, Carole Feurer, Nicolas Radomski, Ludovic Mallet, Michel-Yves Mistou, Sabrina Cadel-Six
Summary: In this study, the genomic diversity of 304 S. Mbandaka strains isolated from bovine and poultry sectors in north-western France was analyzed. The phylogenomic analysis revealed main clustering by host and emphasized the circulation of 12 different major clones, potentially related to the adaptation of the serovar in the food production chain. The identified mutations characteristic of each major cluster could be used to design useful probes for food safety surveillance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Madeleine De Sousa Violante, Gaetan Podeur, Valerie Michel, Laurent Guillier, Nicolas Radomski, Renaud Lailler, Simon Le Hello, Francois-Xavier Weill, Michel-Yves Mistou, Ludovic Mallet
Summary: This study presents a retrospective genomic analysis of Salmonella enterica subsp. enterica Dublin outbreaks in France between 2015 and 2017. The analysis reveals the importance of geographical distance, transformation steps, and genomic diversity in the evolution of S. Dublin populations. The study also highlights the effectiveness of bacterial surveillance in representing diversity.
NAR GENOMICS AND BIOINFORMATICS
(2022)
Article
Food Science & Technology
Marie Noel Mansour, Joseph Yaghi, Andre El Khoury, Arnaud Felten, Michel-Yves Mistou, Ali Atoui, Nicolas Radomski
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Correction
Microbiology
Yann Sevellec, Sophie A. Granier, Nicolas Radomski, Arnaud Felten, Simon Le Hello, Carole Feurer, Michel-Yves Mistou, Sabrina Cadel-Six
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)