Article
Immunology
Nicole C. Thunes, Haitham H. Mohammed, Jason P. Evenhuis, Ryan S. Lipscomb, David Perez-Pascual, Rebecca J. Stevick, Clayton Birkett, Rachel A. Conrad, Jean-Marc Ghigo, Mark J. McBride
Summary: Flavobacterium columnare is a pathogen that causes columnaris disease in freshwater fish, both in natural water bodies and aquaculture settings. This disease is often fatal, especially in high fish population density, and current control options, such as vaccines, are limited. The type IX secretion system (T9SS) is crucial for the virulence of F. columnare, but the specific virulence factors that are secreted through this system are not fully known. This study found that peptidases, which are commonly known virulence factors in other pathogens, may also play a role in F. columnare virulence.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, Clayton Birkett, Mark J. McBride
Summary: Columnaris disease caused by Flavobacterium columnare is a major challenge for freshwater aquaculture. The virulence factors and control methods of F. columnare are poorly understood. This study revealed that iron acquisition mechanisms, such as siderophores, are not critical virulence factors for F. columnare.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nicole C. Thunes, Rachel A. Conrad, Haitham H. Mohammed, Yongtao Zhu, Paul Barbier, Jason P. Evenhuis, David Perez-Pascual, Jean-Marc Ghigo, Ryan S. Lipscomb, John R. Schneider, Nan Li, Devon H. Erbes, Clayton Birkett, Benjamin R. LaFrentz, Timothy J. Welch, Mark J. McBride
Summary: Flavobacterium columnare, a pathogen that causes columnaris disease in freshwater fish, poses a major threat to sustainable aquaculture. Researchers have identified a genetically manipulable strain, MS-FC-4, which has facilitated the identification of virulence factors. Deletion of core genes eliminates virulence, and several secreted proteins have been identified as important for pathogenicity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Benjamin R. LaFrentz, Stanislava Kralova, Claire R. Burbick, Trevor L. Alexander, Conner W. Phillips, Matt J. Griffin, Geoffrey C. Waldbieser, Julio C. Garcia, Fernanda de Alexandre Sebastiao, Esteban Soto, Thomas P. Loch, Mark R. Liles, Kevin R. Snekvik
Summary: This study determined the taxonomic status of four genetic groups of Flavobacterium columnare using polyphasic and phylogenomic approaches. Phylogenetic analyses demonstrated that the four genetic groups formed well supported and distinct clades within the genus Flavobacterium. Biochemical and physiological characteristics were similar among the groups, but quantitative differences in fatty acid profiles were detected and MALDI-TOF analyses showed unique peaks for each group. Therefore, the names F. covae sp. nov., F. davisii sp. nov., and F. oreochromis sp. nov. are proposed for genetic groups 2, 3, and 4, respectively.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
David Perez-Pascual, Sol Vendrell-Fernandez, Bianca Audrain, Joaquin Bernal-Bayard, Rafael Patino-Navarrete, Vincent Petit, Dimitri Rigaudeau, Jean-Marc Ghigo
Summary: The study focused on host-microbiota-pathogen interactions in rainbow trout, demonstrating the importance of specific probiotic bacterial strains in conferring resistance against Flavobacterium columnare infections.
Article
Immunology
Wenjie Guo, Rui Han, Weizhen Xu, Zijun Lu, Yanwei Li, Xueming Dan, Zequan Mo
Summary: This study investigated the immune protective effects of an inactivated F. columnare vaccine. The results showed that when white oil adjuvant was used as a booster, the relative percent survival (RPS) after 8 weeks of vaccination was 34% and 61%, respectively. Furthermore, the inactivated vaccine comprising an appropriate antigen isolate with white oil adjuvant as a booster can provide effective protection in grass carp.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, David Perez-Pascual, Rebecca J. Stevick, Clayton Birkett, Jean-Marc Ghigo, Mark J. McBride
Summary: Flavobacterium columnare, a costly pathogen in the freshwater fish-farming industry, produces siderophores for iron uptake. This study identifies genes involved in iron uptake and reveals their importance for growth under iron-limited conditions. Mutants with deletions in these genes showed decreased virulence but still caused infection. Survivors of exposure to these mutants were partially protected against later infection by the wild-type pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Hui Wang, Xi Chen, Sisi Li, Chengchong Zhou, Lili Xu, Zhixin Wu, Xiaoxuan Chen
Summary: Langerhans cells (LCs) in grass carp were identified for the first time using immunofluorescence and transmission electron microscopy, and a CD207 gene similar to human and mouse counterparts was discovered. The CD207 gene was found to be highly expressed in immune organs and played a significant role in the immune response to bacterial infections.
Article
Microbiology
Jason P. Evenhuis, Ryan Lipscomb, Clayton Birkett
Summary: Flavobacterium columnare, a problematic pathogen in the aquaculture industry, is commonly studied through suspended growth in low nutrient media. The presence of calcium and magnesium significantly affects growth, virulence, and biofilm formation of F. columnare. Different genomovars of F. columnare exhibit varying growth phenotypes under different TYES media conditions, demonstrating phenotypic differences within this bacterial species.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Rui Han, Yuhao Hong, Ruilong Xu, Wenjie Guo, Mingshu Zhang, Zijun Lu, Qing Han, Zequan Mo, Xueming Dan, Yanwei Li
Summary: This study characterized the pathogen Flavobacterium columnare, the causative agent of columnaris disease in freshwater fish, using whole-genome sequencing. The genomes of two isolates showed evidence of gene variation and horizontal transfer, which are important for genetic diversity, genome plasticity, and functional evolution. Genetic analysis clustered F. columnare isolates into distinct clades, supporting a proposed new taxonomic perspective. The findings provided genomic evidence for gene variation and horizontal transfer, and supported a new classification view on F. columnare.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Wei-Dan Jiang, Shuang-An Li, Hai-Feng Mi, Lu Zhang, Lin Feng, Pei Wu, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Cheng-Bo Zhong, Xiao-Qiu Zhou
Summary: The study showed that optimal intake of myo-inositol decreased oxidative stress, inhibited cell apoptosis, promoted cell proliferation, and maintained intercellular structural integrity in the gills of grass carp, possibly through various signaling pathways. The calculated optimal myo-inositol intake for grass carp was 285.8 and 305.4 mg/kg diet, respectively.
Article
Immunology
Yuan Yuan Yang, Si Yao Zheng, Hong Fang, Xiao Man Wu, Jie Zhang, Ming Xian Chang
Summary: This study investigated the effects of different variants of histone H2A in grass carp on Flavobacterium columnare infection. It was found that some variants had antibacterial activity against F. columnare and could enhance the innate immune responses in grass carp. Grass carp immunized with these variants showed lower mortality and fewer numbers of F. columnare. These findings suggest that histone H2A variants play an important role in enhancing disease resistance in fish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Putita Chokmangmeepisarn, Patcharapong Thangsunan, Pattanapon Kayansamruaj, Channarong Rodkhum
Summary: The study provided a thorough understanding of quinolone resistance mechanisms in F. columnare, offering guidance for selecting antibiotics for the treatment of columnaris disease.
Article
Fisheries
Nantawut Ponpukdee, Pradit Wangman, Channarong Rodkhum, Chalinan Pengsuk, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: Monoclonal antibodies generated from outer membrane proteins of pathogenic Flavobacterium columnare can be used as a simple, fast, specific, and low-cost immunological tool for the direct detection of F. columnare without the need for bacterial isolation as in conventional biochemical tests or DNA extraction as in PCR assays. By combining specific MAbs, the detection limit in dot blotting assay can be increased to 5 x 10(2) cfu/dot.
Article
Fisheries
Guojing Xu, Jinlu Zhang, Rufang Ma, Chao Wang, Huizhong Cheng, Junxia Gong, Zhizhong Wang, Qinglei Meng
Summary: The study demonstrated that both bath and intraperitoneal immunization up-regulated pIgR and secretory Ig expression in secretions, providing more insights into the role of pIgR in immunity and offering insight into ways of protecting teleost against pathogen invasion.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Microbiology
Hasan C. Tekedar, Hossam Abdelhamed, Salih Kumru, Jochen Blom, Attila Karsi, Mark L. Lawrence
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Safak Kalindamar, Jingjun Lu, Hossam Abdelhamed, Hasan C. Tekedar, Mark L. Lawrence, Attila Karsi
Article
Agriculture, Dairy & Animal Science
Claudia D. Castaneda, Christopher D. McDaniel, Hossam Abdelhamed, Attila Karsi, Aaron S. Kiess
Article
Fisheries
Hossam Abdelhamed, Ozan Ozdemir, Iman Ibrahim, Mark Lawrence, Attila Karsi
Article
Multidisciplinary Sciences
Hasan C. Tekedar, Salih Kumru, Jochen Blom, Andy D. Perkins, Matt J. Griffin, Hossam Abdelhamed, Attila Karsi, Mark L. Lawrence
Article
Food Science & Technology
Hossam Abdelhamed, Mark L. Lawrence, Reshma Ramachandran, Attila Karsi
Article
Immunology
Adef O. Kordon, Safak Kalindamar, Kara Majors, Hossam Abdelhamed, Wei Tan, Attila Karsi, Lesya M. Pinchuk
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Cell Biology
Safak Kalindamar, Adef O. Kordon, Hossam Abdelhamed, Wei Tan, Lesya M. Pinchuk, Attila Karsi
CELLULAR MICROBIOLOGY
(2020)
Article
Genetics & Heredity
Hasan C. Tekedar, Jochen Blom, Safak Kalindamar, Seongwon Nho, Attila Karsi, Mark L. Lawrence
MICROBIAL GENOMICS
(2020)
Article
Fisheries
Mohamed Sayed, Ozan Ozdemir, Manal Essa, Alicia Olivier, Attila Karsi, Mark L. Lawrence, Hossam Abdelhamed
Summary: This study constructed two in-frame deletion mutants of Edwardsiella piscicida strain C07-087 by deleting the phoP (ETAC_09785) and phoQ (ETAC_09790) genes. The mutants showed delayed adaptation to oxidative stress and decreased biofilm formation, as well as significantly reduced virulence in channel catfish and increased protection efficacy after vaccination. The results suggest that PhoP and PhoQ are crucial for bacterial adaptation and infection in catfish.
JOURNAL OF FISH DISEASES
(2021)
Review
Fisheries
Adef Othan Kordon, Lesya Pinchuk, Attila Karsi
Summary: The immune system of jawed vertebrates consists of two major subsystems: the non-specific innate immune system and the specific adaptive immune system. The adaptive immune system in teleost fish allows for immunological memory and vaccination, making it crucial for disease control in aquaculture.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2022)
Article
Fisheries
Mohamed Sayed, Matt Griffin, Cynthia Ware, Ozan Ozdemir, Hasan C. Tekedar, Manal Essa, Attila Karsi, Mark L. Lawrence, Hossam Abdelhamed
Summary: This study characterizes the role of the BasS/BasR two-component system in Edwardsiella piscicida virulence and immune protection in catfish hosts. The mutants lacking basS and basR genes in E. piscicida were more sensitive to acidic environments and produced less biofilm. In vivo studies revealed significant attenuation and protection in vaccinated catfish. Further investigation is needed to determine the efficacy in hybrid catfish. These findings provide insights into the virulence mechanism of E. piscicida and suggest the potential of using Ep Delta basS and Ep Delta basR strains as vaccines.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Mohamed Sayed, Lakshmi Narayanan, Manal Essa, Mark Lawrence, Attila Karsi, Hossam Abdelhamed
Summary: Columnaris disease caused by Flavobacterium covae leads to significant economic losses in commercially important fish species worldwide. The US channel catfish industry is particularly vulnerable. Therefore, developing a vaccine to reduce the economic losses is urgently needed. This study aims to identify the main secreted extracellular products (SEPs) of F. covae and evaluate their potential for providing protection against columnaris disease in channel catfish.
Article
Microbiology
Eda Ozdemir, Hossam Abdelhamed, Ozan Ozdemir, Mark Lawrence, Attila Karsi
Summary: Virulent Aeromonas hydrophila (vAh) strains causing motile Aeromonas septicemia (MAS) in farmed channel catfish have posed a significant problem for over a decade. The pathogenicity of vAh in catfish is not well understood, which makes studying it crucial. In this study, a bioluminescent vAh (BvAh) was developed using a new bioluminescence expression plasmid and its interactions with catfish were visualized using bioluminescent imaging. The findings provide valuable insights into the pathogenicity of vAh in catfish and demonstrate potential entry portals for vAh.
Article
Immunology
Adef O. Kordon, Hossam Abdelhamed, Hamada Ahmed, Wes Baumgartner, Attila Karsi, Lesya M. Pinchuk
FRONTIERS IN IMMUNOLOGY
(2019)