Article
Agriculture, Dairy & Animal Science
Yan Zhang, Bo Liu, Abdelrahman Said, Jinwen Xie, Fengrong Tian, Zongxi Cao, Zhe Chao, Feng Li, Xin Li, Shuguang Li, Hailong Liu, Wenxiu Wang
Summary: Our study revealed the crucial role of the NLRP3 inflammasome in managing the invasion of respiratory mycoplasma in swine. The findings suggest that NLRP3 expression levels can serve as a reliable diagnostic indicator for identifying Mycoplasma hyopneumoniae infection. Additionally, inhibiting NLRP3 could offer therapeutic advantages in treating NLRP3-related disorders, such as porcine enzootic pneumonia.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Microbiology
Leonardo Teofilo Toledo, Luiz Fernando Lino de Souza, Carlos Eduardo Real Pereira, Richard Costa Polveiro, Gustavo Costa Bressan, Ricardo Seiti Yamatogi, Kwangcheol Casey Jeong, Fernanda Simone Marks, Caio Augustus Diamantino, Victor Hugo Rabelo de Carvalho, Clarisse Sena Malcher, Fernando Antonio Moreira Petri, Luis Guilherme de Oliveira, Maria Aparecida Scatamburlo Moreira, Abelardo Silva-Junior
Summary: This study conducted a complete sequencing analysis of two Brazilian strains of Mycoplasma hyopneumoniae and identified the virulence factors and sequence type of the isolates. The pathogenicity of the isolates was then evaluated in a swine model, revealing that one of the strains exhibited higher virulence than the other.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Hui Zhang, Yuanyuan Wang, Lu Gao, Yan Wang, Rong Wei
Summary: This study reported the positivity rates and genotypes of Mycoplasma hyopneumoniae in pig herds in China for the first time. MLST analysis revealed high genotype diversity among M. hyopneumoniae from different provinces in China.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Henrique Meiroz de Souza Almeida, Karina Sonalio, Marina Lopes Mechler-Dreibi, Fernando Antonio Moreira Petri, Gabriel Yuri Storino, Dominiek Maes, Luis Guilherme de Oliveira
Summary: Mycoplasma hyopneumoniae infection can alter the diversity of the lower respiratory microbiota and increase the susceptibility of pigs to respiratory infections.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qi Zhou, Kaijie Mai, Dehong Yang, Junfa Liu, Zhuanqiang Yan, Cuifen Luo, Yangtong Tan, Sheng Cao, Qingfeng Zhou, Li Chen, Feng Chen
Summary: Sequencing and analysis of 15 Mycoplasma anatis strains revealed the genomic structure and functional characteristics, identifying numerous virulence-related genes, most of which are highly conserved across all strains. This information provides insights into molecular mechanisms of drug resistance, adaptive evolution of pathogens, population structure, and vaccine development.
Article
Infectious Diseases
Yapei Rui, Gang Qiu
Summary: The susceptibility breakpoint of M.hyo to enrofloxacin was determined to be 1μg/mL.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Veterinary Sciences
Henrique M. S. Almeida, Marina L. Mechler-Dreibi, Karina Sonalio, Marcela M. Ferreira, Paulo E. B. Martinelli, Igor R. H. Gatto, Dominiek Maes, Helio J. Montassier, Luis G. Oliveira
Summary: Direct detection of Mycoplasma hyopneumoniae through molecular tools is important for early diagnosis, with the study showing variations in pathogen loads and lesion severity in different anatomical sites of the respiratory tract over time in experimentally infected swine. These findings are crucial for improving diagnostics, treatment, and control measures for M. hyopneumoniae infection in swine herds.
PORCINE HEALTH MANAGEMENT
(2021)
Article
Immunology
Hongliang Zhang, Ruihua Zhang, Fengxue Wang, Guimei Li, Yongjun Wen, Hu Shan
Summary: This study compared the proteomes of PK15 cells infected by a PRV variant and a vaccine strain using TMT technology. Differentially expressed proteins were identified, providing new insights into the mechanisms of PRV pathogenesis and immune evasion.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Heloise Pageaut, Sonia Lacouture, Melanie Lehoux, Corinne Marois-Crehan, Mariela Segura, Marcelo Gottschalk
Summary: Bacterial and viral co-infections are common in swine production, causing economic losses. This study investigated the interactions between M. hyopneumoniae, M. hyorhinis, and S. suis in swine cells. The co-infection led to increased cellular toxicity and release of pro-inflammatory cytokines.
Article
Veterinary Sciences
Zhenya Li, Yingxin Wang, Yanyan Zhang, Xibiao Tang, Xiangru Wang, Wenhao Liu, Yulin Qian, Yongwei Zhu, Huanchun Chen, Chen Tan
Summary: In this study, an attenuated strain of Mycoplasma hyopneumoniae was produced by serial passaging a highly virulent strain in vitro, and genomic analysis revealed large gene deletions and single-nucleotide variations in the attenuated strain compared to the virulent strain. These mutations may be associated with the decreased virulence of the attenuated strain.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Xue Luo, Mo Li, Henan Zhang, Danli Yan, Shuaiqi Ji, Rina Wu, Yongfu Chen
Summary: Proteomics and bioinformatics analysis of three strains of Lactobacillus plantarum under salt stress revealed differential expression of proteins related to protein biosynthesis, nucleotide metabolism, and sugar metabolism. Specific differentially expressed proteins were found between the strains in response to high salt conditions.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agriculture, Dairy & Animal Science
Wolfgang Sipos, Sabine Sipos
Summary: In this field study conducted in a pig farm in Lower Austria, the clinical efficacy of two novel vaccines against enzootic pneumonia (EP) was tested. It was found that both vaccines were effective in combating EP.
Article
Biochemistry & Molecular Biology
Chenxing Tan, Chuhao Li, Ming Hu, Anqun Hu, Yang Xue, Xiaofan Zhou, Jianuan Zhou
Summary: Dickeya dadantii subsp. paradisiaca strain FZ06 has a lower virulence to banana and potato, but a higher virulence to Chinese cabbage and onion compared to D. zeae MS2. Strain FZ06 shows significantly less production of pectate lyases, polygalacturonases, proteases, and extrapolysaccharides, as well as lower swimming and swarming motility and biofilm formation. Genomic comparison reveals the presence of additional gene clusters in FZ06, including Stt-type T2SS, three T4SSs, and one T4P.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Peng Lu, Jiaqiao Zhou, Sibo Wei, Konosuke Takada, Hayato Masutani, Suguru Okuda, Ken Okamoto, Michio Suzuki, Tomoya Kitamura, Kentaro Masujin, Takehiro Kokuho, Hideaki Itoh, Koji Nagata
Summary: African swine fever (ASF) is a devastating disease caused by the African swine fever virus (ASFV) that threatens the pig industry and food security worldwide. This study identified potential vaccine targets for developing a broad-spectrum defense against ASFV by analyzing genomic and transcriptomic data of different ASFV strains. The results provide valuable candidate ORFs for ASF vaccine development.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Agriculture, Dairy & Animal Science
Jacquelyn Horsington, Maarten Witvliet, Antonius A. C. Jacobs, Ruud P. A. M. Segers
Summary: This study found that simultaneously vaccinating against the four major pathogens in swine using intradermal application had equivalent efficacy to single vaccinations. The combined vaccination method offers benefits such as reduced time and labor costs, as well as improved animal welfare.
Article
Biotechnology & Applied Microbiology
Gabriela Prado Paludo, Claudia Elizabeth Thompson, Kendi Nishino Miyamoto, Rafael Lucas Muniz Guedes, Arnaldo Zaha, Ana Tereza Ribeiro de Vasconcelos, Martin Cancela, Henrique Bunselmeyer Ferreira
Article
Multidisciplinary Sciences
Scheila G. Mucha, Mariana G. Ferrarini, Carol Moraga, Alex Di Genova, Laurent Guyon, Florence Tardy, Sophie Rome, Marie-France Sagot, Arnaldo Zaha
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Joao Antonio Debarba, Martin Pablo Cancela Sehabiague, Karina Mariante Monteiro, Alexandra Lehmkuhl Gerber, Ana Tereza Ribeiro Vasconcelos, Henrique Bunselmeyer Ferreira, Arnaldo Zaha
Article
Oncology
Helier Balbinotti, Nathan A. Cadore, Cristine S. Dutra, Edileuza D. da Silva, Henrique B. Ferreira, Arnaldo Zaha, Karina M. Monteiro
ANTICANCER RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
F. M. Siqueira, S. De Carli, C. E. Lopes, L. Machado, T. R. Vieira, A. G. Poppl, M. R. Cardoso, A. Zaha
Summary: The study found that non-lactose-fermenting E. coli from dogs with hyperadrenocorticism behaves similarly to lactose-fermenting E. coli in terms of virulence, biofilm formation capacity, and antimicrobial susceptibility, exhibiting multidrug resistance. These strains have the pathogenic potential to promote animal infections.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Parasitology
Paula Arbildi, Silvana La-Rocca, Alejandra Kun, Karina R. Lorenzatto, Karina M. Monteiro, Arnaldo Zaha, Gustavo Mourglia-Ettlin, Henrique B. Ferreira, Veronica Fernandez
Summary: The study revealed the important roles of GSTs in E. granulosus s.l. biology, particularly in parasite establishment and detoxification processes. EgGST1 was found to be associated with detoxification, with increased expression in the parenchyma of pre-adult protoscoleces (PSCs); EgGST2, located on the tegument of PSCs, showed increased expression post-anthelmintic treatment, potentially related to immunoregulation and chemotherapy resistance; EgGST3, related to the Omega class, exhibited distribution throughout the PSC body and increased expression in response to oxidative stress.
Article
Immunology
Bryan Augusto da Rosa Tavares, Jessica Andrade Paes, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
Summary: This study reannotated the function of unknown proteins in M. hyopneumoniae using available databases and bioinformatic tools, predicting new potential virulence factors and improving the functional annotation of the genome.
MICROBIAL PATHOGENESIS
(2022)
Article
Parasitology
Guilherme Brzoskowski dos Santos, Edileuza Danieli da Silva, Eduardo Shigueo Kitano, Maria Eduarda Battistella, Karina Mariante Monteiro, Jeferson Camargo de Lima, Henrique Bunselmeyer Ferreira, Solange Maria de Toledo Serrano, Arnaldo Zaha
Summary: This study investigated the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts and compared their molecular arsenal at the host-parasite interface. The results showed a predominance of parasite proteins compared to host proteins in the hydatid fluid samples. Functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Karina Colonetti, Evelise Leis de Carvalho, Darlene Lopes Rangel, Paulo Marcos Pinto, Luiz Fernando Wurdig Roesch, Franciele Cabral Pinheiro, Ida Vanessa Doederlein Schwartz
Summary: In this study, the fecal metabolites of GSD patients were measured and compared to healthy controls. The results showed higher levels of succinic acid in GSD patients, while no significant differences were observed for other metabolites. Fecal pH was found to be inversely correlated with lactic acid, and certain microbial structures were affected by propionic and lactic acid. Overall, there were no correlations between short-chain fatty acids and carbohydrate consumption.
Article
Pharmacology & Pharmacy
Darlene Lopes Rangel, Rafael D. Melani, Evelise Leis Carvalho, Juliano Tomazzoni Boldo, Tiago Gomes dos Santos, Neil L. Kelleher, Paulo Marcos Pinto
Summary: Envenomation from the Bothrops genus is characterized by the effects of toxin families in the venom, which can be identified using proteomics approaches. Various protein groups, proteoforms, and multi-proteoform complexes have been identified in Bothrops pubescens venom.
Article
Multidisciplinary Sciences
Priscila Souza dos Santos, Jessica Andrade Paes, Lais Del Pra Netto Machado, Gabriela Prado Paludo, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
Summary: Mycoplasma hyopneumoniae possesses surface proteins of unknown function (PUFs) that may play a role in the pathogenicity of porcine enzootic pneumonia (PEP). This study compared the expression of five PUFs in the pathogenic strain 7448 of M. hyopneumoniae with their orthologs from nonpathogenic M. hyopneumoniae J strain and a closely related commensal species Mycoplasma flocculare. Analysis of amino acid sequences and proteomic data revealed differential domains, disordered regions, and repeated motifs. Differential endoproteolytic processing and antigenicity were also observed. Phylogenetic analysis showed higher conservation of three PUFs among Mycoplasma species related to respiratory diseases.
Article
Parasitology
Marcelo Pasa Panesso, Martin Cancela, Renato Kulakowski Cora, Jessica Andrade Paes, Gabriela Prado Paludo, Henrique Bunselmeyer Ferreira
Summary: This study evaluates the potential of nucleotide salvage pathways in cestodes and other parasitic helminths as drug targets for anthelmintic repurposing. By conducting genomic surveys and evolutionary analyses, the researchers identified specific enzymes that could be potential targets for anthelmintic drugs. In vitro experiments confirmed the effectiveness of a nucleotide salvage inhibitor against the cestode.
EXPERIMENTAL PARASITOLOGY
(2023)
Review
Immunology
Fernanda M. A. Leal Zimmer, Jessica Andrade Paes, Arnaldo Zaha, Henrique Bunselmeyer Ferreira