Article
Chemistry, Physical
Feng Cao, Yinan Li, Jiahao Wu, Wei Liu, To Ngai
Summary: This study investigated the interactions between fluorescein isothiocyanate (FITC) and PEG SAMs at different ionic strengths. It found long-range attractions at low ionic strength and increased van der Waals attractions at high ionic strength. The results reveal different interaction mechanisms at different ionic strengths.
Article
Developmental Biology
Zengyu Liu, Isabella Osterlund, Felix Ruhnow, Yiran Cao, Guoqiang Huang, Wenguo Cai, Jiao Zhang, Wanqi Liang, Zoran Nikoloski, Staffan Persson, Dabing Zhang
Summary: Rice is an important food crop, and the cytoskeleton plays a crucial role in its growth, development, and response to the environment. However, reliable markers for the cytoskeleton in rice have been lacking, limiting our understanding of its functions. In this study, fluorescent marker lines for the actin and microtubule cytoskeletons in rice were generated, and their roles were investigated in pollen grain development, root cell development, and salt stress response.
Article
Biochemistry & Molecular Biology
Thais Kroetz, Pablo Andrei Nogara, Fabiano da Silveira Santos, Lilian Camargo da Luz, Viktor Saraiva Camara, Joao Batista Teixeira da Rocha, Alexandre Goncalves Dal-Bo, Fabiano Severo Rodembusch
Summary: The study examines the interaction of ESIPT fluorescent lipophile-based benzazoles with BSA, finding that these compounds bind to BSA through a strong binding affinity provided by a static quenching mechanism. The interactions were studied using UV-Vis absorption and steady-state fluorescence spectroscopy, showing that the compounds exhibit a large Stokes shift and absorption in the UV region. Additionally, docking simulations suggest different binding poses and interactions of the compounds with BSA domains.
Article
Engineering, Biomedical
Antonio Pallotti, Giancarlo Orengo, Giovanni Saggio
Summary: This study compared the finger joint angle measurements obtained from a myoelectric armband and a data glove, showing similar performance of the two wearable devices. The results demonstrated that myoelectric armbands can be used to accurately register static postures of finger joints.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Christoph Guschlbauer, Scott L. Hooper, Charalampos Mantziaris, Anna Schwarz, Nicholas S. Szczecinski, Ansgar Buschges
Summary: Due to scaling issues, the importance of passive muscle and joint forces increases as limb size decreases. The resting-state range of passive forces is predicted to correlate with the limb's typical working range in locomotion, which could simplify motor control and save energy. This study provides evidence of how evolution can alter behavior by changing passive muscle and joint properties.
Article
Nanoscience & Nanotechnology
Yanling Chen, Sanyin Qu, Qingfeng Song, Wei Shi, Hui Li, Qin Yao, Lidong Chen
Summary: By introducing nanoscale phase separations and tailoring nanoscale interfaces, the thermoelectric performance of organic small molecules can be optimized, achieving synergistically optimized electrical and thermal transport properties.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Multidisciplinary
Robert Lipton, Paul Sinz, Michael Stuebner
Summary: This paper demonstrates the accuracy of the Generalized Finite Element Methods (GFEM) in resolving local features in heterogeneous media and provides a geometric explanation for the nearly optimal approximation properties of local bases.
COMPUTER METHODS IN APPLIED MECHANICS AND ENGINEERING
(2022)
Article
Automation & Control Systems
Yunpeng Hu, Inna Sharf, Lei Chen
Summary: This paper presents a novel solution for cooperative autonomous orbit determination for a satellite constellation using only inertial angles measurements, and establishes a relationship between observability and a graph representation of measurements among spacecraft. By studying the topological properties of measurement graphs, the number of spacecraft, and orbital configurations, the degree of system observability is demonstrated.
Article
Chemistry, Physical
Rafal Wysokinski, Wiktor Zierkiewicz, Mariusz Michalczyk, Steve Scheiner
Summary: On a naphthalene scaffold, PnF(2) can interact with an NH2 group on the adjoining ring through an internal pnicogen bond. The presence of an approaching NH3 molecule weakens the intramolecular bond. Substituting the external NH3 with a CN- anion leads to a rearrangement of the bonding pattern, forming a fourth covalent bond with Pn.
Article
Plant Sciences
Z. Carter Berry, Jerry Larue, Gregory Goldsmith
Summary: This study quantified the angular distribution of light from common gas exchange systems and demonstrated the use of an integrating sphere for manipulating light distributions. The integrating sphere can create light environments that more closely mimic variations in sunlight. Different proportions of diffuse light have varying effects on photosynthetic rates in different plant species.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Chemistry, Physical
Hannah R. Shanks, Shanglin Wu, Nam T. Nguyen, Dongdong Lu, Brian R. Saunders
Summary: This study demonstrates the remote and non-invasive monitoring of strain within injectable gels using fluorescent particle probes. The normalized photoluminescence intensity from blue emitting probe is shown to correlate with compressive deformation ratio, while the ratio of PL intensities for blue and NIR probes linearly varies with deformation ratio. This approach has the potential to discriminate between compression and tension and enable remote detection of gel status.
Article
Acoustics
Jessica A. Peterson, Isaiah Smolar, Guillaume Stoffels, Angela Bianco
Summary: This study evaluates the variability of transvaginal (TV) and transabdominal (TA) cervical length (CL) measurements and patient factors associated with the accuracy of TA CL measurements. The results show that there is a significant difference between TA and TV CL measurements, especially when the CL is greater than 3.4 cm. Multivariable analysis indicates that Hispanic ethnicity is not associated with the accuracy of TA CL measurements. The study suggests using the TV method for CL measurements below 3.4 cm to improve accuracy.
JOURNAL OF ULTRASOUND IN MEDICINE
(2023)
Article
Chemistry, Analytical
Izaak Van Crombrugge, Seppe Sels, Bart Ribbens, Gunther Steenackers, Rudi Penne, Steve Vanlanduit
Summary: This study tested three methods for calculating 3D skeletons from 2D detections and compared them using recordings and a marker-based tracking system, demonstrating that these methods are accurate enough to calculate a useful ergonomic score.
Article
Multidisciplinary Sciences
Manon Gourdelier, Jitendriya Swain, Coline Arone, Anita Mouttou, David Bracquemond, Peggy Merida, Saveez Saffarian, Sebastien Lyonnais, Cyril Favard, Delphine Muriaux
Summary: This study provides new insights on the use of non-fluorescent and fluorescent VLPs to study and visualize the SARS-CoV-2 viral life cycle in a safe environment. Moreover, optimized SARS-CoV-2 VLP production can be further adapted to vaccine design strategies.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Saquib Ahmed M. A. Peerzade, Nadezhda Makarova, Igor Sokolov
Summary: The mesoporous nature of silica nanoparticles allows for the development of ultrabright fluorescent particles by physically encapsulating organic molecular fluorescent dyes inside the pores. These particles can be used to assemble ultrabright fluorescent ratiometric sensors for simultaneous pH and temperature measurements with high sensitivity and stability.
Article
Chemistry, Multidisciplinary
Eva C. Arnspang, Prabuddha Sengupta, Kim I. Mortensen, Helene H. Jensen, Ute Hahn, Eva B. V. Jensen, Jennifer Lippincott-Schwartz, Lene N. Nejsum
Article
Chemistry, Multidisciplinary
Murat Nulati Yesibolati, Kim Mortensen, Hongyu Sun, Anders Brostrom, Sofie Tidemand-Lichtenberg, Kristian Molhave
Article
Physics, Multidisciplinary
Kim I. Mortensen, Henrik Flyvbjerg, Jonas N. Pedersen
Summary: This study presents explicit and exact expressions for measuring and mean-squared displacement of Brownian particles confined in different geometries, valid for all exposure times. The results apply not only to simple geometries, but also have generality for more complex shapes. The exact quantitative descriptions provided here can offer a qualitative and semi-quantitative understanding of a wide range of physics phenomena.
FRONTIERS IN PHYSICS
(2021)
Article
Physics, Multidisciplinary
Martin Schmidt, Adam C. Hundahl, Henrik Flyvbjerg, Rodolphe Marie, Kim I. Mortensen
Summary: Recent studies show that structured illumination plays an important role in super-resolution localization and tracking of fluorescent particles, combining conventional localization information significantly improves precision.
COMMUNICATIONS PHYSICS
(2021)
News Item
Physics, Multidisciplinary
Henrik Flyvbjerg
Summary: A study shows that cells prefer regions they have already visited when moving on microprinted tracks, suggesting a potential update to century-old dynamical models for cell trajectories.
Article
Chemistry, Physical
Denis Selnihhin, Kim Mortensen, Jannik B. Larsen, Jens B. Simonsen, Finn Skou Pedersen
Summary: In this study, DNA-origami nanobeads were designed and constructed using DNA nanotechnology for fluorescence/antigen quantification in flow cytometry. The effectiveness of the bead design was verified through various microscopy techniques. The results demonstrate that DNA beads can accelerate quantitative studies of the biology of individual particles in flow cytometry.
Article
Chemistry, Multidisciplinary
Aleksandra K. Adamczyk, Teun A. P. M. Huijben, Miguel Sison, Andrea Di Luca, Stefano Vanni, Sophie Brasselet, Kim I. Mortensen, Fernando D. Stefani, Mauricio Pilo-Pais, Guillermo P. Acuna
Summary: This study demonstrates a controlled orientation of single molecules in DNA origami by linking them to oligonucleotide strands and leaving unpaired bases. The number of unpaired bases affects the stretching and orientation of the fluorophore linkers. These findings expand the application of DNA origami in the fabrication of nanodevices involving orientation-dependent molecular interactions.
Article
Chemistry, Multidisciplinary
Adam Coln Hundahl, Arjen Weller, Jannik Bruun Larsen, Claudia U. Hj, Morten B. Hansen, Ann-Kathrin Muendler, Astrid Knuhtsen, Kasper Kristensen, Eva C. Arnspang, Thomas Lars Andresen, Kim I. Mortensen, Rodolphe Marie
Summary: Oral drug delivery is the preferred administration route for most drugs, but it is limited for biologics due to the intestinal barrier. One strategy to improve the absorption is chemical modification through lipidation. However, the mechanistic understanding of the effect is largely unexplored. This study developed a method to quantify peptide transport through a monolayer of Caco-2 cells and investigated the effects of lipidation on transport mechanism.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemical Research Methods
Milan Finn Wesseler, Mathias Norbaek Johansen, Aysel Kiziltay, Kim Mortensen, Niels B. Larsen
Summary: This paper proposes a non-contact 3D mapping method for oxygen tension using commercially available oxygen microsensor beads embedded in confocal phosphorescence lifetime microscopy. The method accurately evaluates oxygen distribution in high cell-density cultures. Its versatility is demonstrated in the design and control of microperfused 3D printed hydrogel culture chips.
Review
Biochemistry & Molecular Biology
Jannik Bruun Larsen, Nayere Taebnia, Alireza Dolatshahi-Pirouz, Anne Zebitz Eriksen, Claudia Hjorringgaard, Kasper Kristensen, Nanna Wichmann Larsen, Niels Bent Larsen, Rodolphe Marie, Ann-Kathrin Mundler, Ladan Parhamifar, Andrew James Urquhart, Arjen Weller, Kim I. Mortensen, Henrik Flyvbjerg, Thomas Lars Andresen
Summary: Oral delivery is preferred by patients, but poses challenges for drug classes like pharmaceutical peptides due to biological barriers. Improving peptide transport across the intestinal barrier is crucial for future treatments. Fluorescence imaging allows direct visualization of peptide transport, providing detailed mechanistic understanding.
RSC CHEMICAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jakob L. Kure, Camilla B. Andersen, Kim I. Mortensen, Paul W. Wiseman, Eva C. Arnspang
Meeting Abstract
Biochemistry & Molecular Biology
J. Lopacinska-Jorgensen, J. N. Pedersen, M. Bak, M. Mehrjouy, P. Ostergaard, A. Kristensen, R. Taboryski, H. Flyvbjerg, R. Marie, N. Tommerup, A. Silahtaroglu
EUROPEAN JOURNAL OF HUMAN GENETICS
(2018)