Article
Nanoscience & Nanotechnology
Xiuli Zhang, Wenbo Zhai, Li Fan, Franklin Kim, Yi Yu
Summary: This study investigates the dynamics of confined liquid using liquid-cell transmission electron microscopy. The results show that the spreading of the liquid leads to membrane bending and contraction of nanoparticles. Additionally, the directional movement of the liquid layer also causes cooperative motion of the nanoparticles.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Materials Science, Multidisciplinary
Y. Ikeda, R. Yuan, A. Chakraborty, H. Ghassemi-Armaki, J. M. Zuo, R. Maass
Summary: The study revealed the presence of nano-scale intermetallic phase G-phase in uncracked grain boundaries, suggesting it as one of the primary nanoscopic drivers for grain-boundary weakening and crack initiation. These findings shift the focus of LME research to the very early stages immediately following Zn diffusion, where secondary phase nucleation and growth emerge as the root-cause for failure.
MATERIALS TODAY ADVANCES
(2022)
Article
Mechanics
Pavel Castro-Villarreal, Claudio Contreras-Aburto, Sendic Estrada-Jimenez, Idrish Huet-Hernandez, Oscar Vazquez-Rodriguez
Summary: The study examines the stochastic dynamics of a tagged Brownian particle in an interacting system using the path-integral representation and contrasts it with the standard equations. It investigates the single-file diffusion phenomenon and the mean-square displacement of the tracer particle in terms of Bessel functions. Additionally, it explores the behavior of a Brownian particle system with paramagnetic interactions near crystallization using a perturbation treatment and validates the findings through Brownian dynamics simulation.
JOURNAL OF STATISTICAL MECHANICS-THEORY AND EXPERIMENT
(2021)
Article
Chemistry, Multidisciplinary
Abhijit H. Phakatkar, Vitaliy Yurkiv, Pankaj Ghildiyal, Yujie Wang, Azadeh Amiri, Lioudmila V. Sorokina, Michael R. Zachariah, Tolou Shokuhfar, Reza Shahbazian-Yassar
Summary: In this study, the interaction of multi-principal elemental alloy nanoparticles with Escherichia coli bacteria was evaluated using the in situ graphene liquid cell scanning transmission electron microscopy approach. The nanoparticles caused damage to the bacteria wall and resulted in cytoplasmic leakage. The release of metal ions from the nanoparticles showed selectivity, with copper ions having the highest release, followed by nickel ions, and iron ions having the lowest release. The binding affinity of bacterial cell membrane protein functional groups with the metal cations drove the selective release of metal ions from the nanoparticles.
Article
Chemistry, Physical
Hanglong Wu, Hao Su, Rick R. M. Joosten, Arthur D. A. Keizer, Laura S. van Hazendonk, Maarten J. M. Wirix, Joseph P. Patterson, Jozua Laven, Gijsbertus de With, Heiner Friedrich
Summary: The study presents methods to quantitatively map and dynamically modulate liquid layer thickness in Liquid-Phase (Scanning) Transmission Electron Microscopy (LP-(S)TEM) experiments, aiming to achieve high-resolution imaging and overcome diffusion limitations.
Article
Food Science & Technology
Amal S. Albedwawi, Reem Al Sakkaf, Tareq M. Osaili, Ahmed Yusuf, Amin Olaimat, Shao-Quan Liu, Giovanni Palmisano, Nagendra P. Shah, Mutamed M. Ayyash
Summary: This paper investigated the ability of lactic acid bacteria (LAB) and Bifidobacterium to remove acrylamide (AA) and examined the mechanism(s) of the AA removal using various microscopic techniques. The results showed that Bifidobacterium breve and Lactiplantibacillus plantarum exhibited higher acrylamide reduction rates. The study also found that both strains could tolerate low pH levels and remove acrylamide under in vitro gastrointestinal conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
X. L. Lu, J. Wang, Q. Y. Huang, J. S. Chen, B. Z. Sun
Summary: This study investigates a phase transition from ss S' to ss 1 in a Mg-Y-Ce alloy caused by electron beam irradiation through atomic scale scanning transmission electron microscopy. It is found that the structural evolution from ss S' to ss 1 precipitates can be completed within two minutes for a nanosized particle, mainly due to the increased heat and vacancy concentration generated by the electron beam irradiation accelerating atomic diffusion, particularly along the c-axis direction.
MATERIALS CHARACTERIZATION
(2022)
Article
Nanoscience & Nanotechnology
Yawei Li, Li Wang, Yufeng He, Wei Zheng, Langhong Lou, Jian Zhang
Summary: The study found that as the temperature increases, the spacing of dislocation networks in the gamma/gamma' interface decreases, while the minimum creep rate increases non-linearly. This is due to the enhanced dislocation-climb in the interface and gamma'-cutting by a <010> superdislocations.
SCRIPTA MATERIALIA
(2022)
Article
Mechanics
P. M. Ndiaye, F. W. Tavares, E. K. Lenzi, L. R. Evangelista, H. Ribeiro, D. Marin, L. M. S. Guilherme, R. S. Zola
Summary: The study focuses on the modeling of diffusion and trapping of particles inspired by biology and natural behavior, particularly in systems connected by surfaces to bulk, such as living cells and biomaterials. The research finds a variety of behaviors through analytical calculations and computer simulations, showing a good agreement between the two methods.
JOURNAL OF STATISTICAL MECHANICS-THEORY AND EXPERIMENT
(2021)
Article
Nanoscience & Nanotechnology
Dongyue Chen, Kenta Murakami, Huilong Yang, Liang Chen, Hiroaki Abe, Zhengcao Li, Naoto Sekimura
Summary: Irradiation flux effects are directly related to the behavior of point defects, which can be indicated by the growth of interstitial loops and the number density of nucleated defects during different flux irradiation. Long-range diffusion of interstitials towards pre-existing sinks plays a significant role in low-flux irradiation, while it is interrupted by rapid defect production in high-flux irradiation. The presence of Si hinders long-range diffusion due to the interstitial trapping effect, reducing the differences between low-flux and high-flux irradiation.
SCRIPTA MATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Yuqing Ye, Keni Cota-Ruiz, Jesus M. Cantu, Carolina Valdes, Jorge L. Gardea-Torresdey
Summary: Engineered nanomaterials interact dynamically with matrices such as soil, water, and biological tissues throughout their lifecycle. Electron microscopy plays a crucial role in assessing these interactions, providing high-resolution imaging capabilities for real-time visualization of nanomaterials in biological matrices. Recent progress in evaluating nanobio interactions using EM has led to advancements in nanoresearch, shedding light on nanocorona formation, lipid nanoparticle analysis, and real-time observation of cellular uptake of nanoparticles.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Nanoscience & Nanotechnology
Jiuyang Dong, Xingyu Feng, Xianchao Hao, Wenjun Kuang
Summary: The Co-free high entropy alloy 28%Fe-27%Ni-27%Mn-18%Cr exhibits good irradiation resistance but is susceptible to stress corrosion cracking, mainly due to the outward diffusion of Mn and Cr during oxidation causing grain boundary migration. The presence of a porous oxide layer, especially when stressed, leads to stress corrosion cracking initiation.
SCRIPTA MATERIALIA
(2021)
Article
Nanoscience & Nanotechnology
Dayne F. Swearer, Stefan Fischer, Daniel K. Angell, Chris Siefe, A. Paul Alivisatos, Steven Chu, Jennifer A. Dionne
Summary: Our study involved the development of a library of 11 unique lanthanide-doped nanophosphors for high-resolution, multicolor electron microscopy. Transmission electron microscopy revealed that these nanophosphors exhibit strong cathodoluminescence spectra under electron beam, with high spatial localization characteristics.
Article
Microbiology
Monika Kakol, Ezher Tagliasacchi, Andrzej Borkowski, Miroslaw Slowakiewicz
Summary: The nutritional condition of seed mussels used in New Zealand's Greenshell mussel aquaculture industry shows high variability, which may contribute to the marked variation observed in spat performance. Environmental factors that limit feeding during harvest and transport of spat are likely influencing the quality of spat used by the industry. Measures to improve the quality of wild spat supply, such as testing nutritional condition and developing nursery culture methods and artificial spat feeds, are needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Electrical & Electronic
Tuan Zaharinie, Zainul Huda, Suriani Ibrahim, Farazila Yusof, Mohd Hamdi, Mohammad Rehan, Tadashi Ariga
Summary: This research paper introduces a reliable and economical method for direct brazing using an active Ag-Cu-Ti filler metal that ensures effective brazing. The study focuses on the sapphire-sapphire brazing process and the formation of uniform reaction layers at the interface. The uniformity of the reaction layers decreases with increasing brazing temperature and time.
ACS APPLIED ELECTRONIC MATERIALS
(2022)
Article
Chemistry, Applied
Wei Huang, Chao Peng, Jing Tang, Fangyuan Diao, Murat Nulati Yesibolati, Hongyu Sun, Christian Engelbrekt, Jingdong Zhang, Xinxin Xiao, Kristian S. Molhave
Summary: In this study, ultrafine Fe3O4 nanoparticles were immobilized on 2D Ni-based MOFs to enhance OER stability and catalytic activity. The optimal Fe3O4/Ni-BDC composite showed the best OER performance with higher overpotential, lower Tafel slope, and considerable durability, facilitated by Fe species with a higher oxidation state for the formation of adsorbed O* species.
JOURNAL OF ENERGY CHEMISTRY
(2022)
Article
Chemistry, Physical
Denis Selnihhin, Kim Mortensen, Jannik B. Larsen, Jens B. Simonsen, Finn Skou Pedersen
Summary: In this study, DNA-origami nanobeads were designed and constructed using DNA nanotechnology for fluorescence/antigen quantification in flow cytometry. The effectiveness of the bead design was verified through various microscopy techniques. The results demonstrate that DNA beads can accelerate quantitative studies of the biology of individual particles in flow cytometry.
Article
Materials Science, Multidisciplinary
Anders Brostrom, Kristian Molhave
Summary: This paper presents an algorithm called Spatial Image Resolution Assessment by Fourier analysis (SIRAF) that estimates resolution directly from a single image using fast Fourier transform analysis. The algorithm is tested on simulated and real images to demonstrate its ability to accurately assess resolution in various microscopy techniques, which can improve focus optimization and magnification settings.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Nanoscience & Nanotechnology
Ganapathi Prabhu Sai Balasubramanian, Elizaveta Lebedkina, Nebile Isik Goktas, Jakob Birkedal Wagner, Ole Hansen, Ray LaPierre, Elizaveta Semenova, Kristian Molhave, Marco Beleggia, Elisabetta Maria Fiordaliso
Summary: Off-axis electron holography was used to observe remote doping in GaAs nanowires during in situ annealing in a transmission electron microscope. Dynamic changes in electrostatic potential caused by carbon dopant diffusion upon annealing were measured, and the electrostatic potential profiles were extracted from GaAs nanowires with radial p-p+ core-shell junctions. The experiment showed that the built-in potentials remained nearly constant and the depletion layer widths slightly increased, contrary to the predictions made in the absence of remote doping. A depression in the potential profile of the core upon annealing was also observed, which was attributed to carbon diffusion from the shell to the core through the nanowire sidewalls.
Article
Chemistry, Multidisciplinary
Aleksandra K. Adamczyk, Teun A. P. M. Huijben, Miguel Sison, Andrea Di Luca, Stefano Vanni, Sophie Brasselet, Kim I. Mortensen, Fernando D. Stefani, Mauricio Pilo-Pais, Guillermo P. Acuna
Summary: This study demonstrates a controlled orientation of single molecules in DNA origami by linking them to oligonucleotide strands and leaving unpaired bases. The number of unpaired bases affects the stretching and orientation of the fluorophore linkers. These findings expand the application of DNA origami in the fabrication of nanodevices involving orientation-dependent molecular interactions.
Article
Toxicology
Claudia Torero Gutierrez, Charis Loizides, Iosif Hafez, Anders Brostrom, Henrik Wolff, Jozef Szarek, Trine Berthing, Alicja Mortensen, Keld Alstrup Jensen, Martin Roursgaard, Anne Thoustrup Saber, Peter Moller, George Biskos, Ulla Vogel
Summary: This study investigated the acute phase response and cardiovascular disease risk associated with exposure to metal oxide nanomaterials. The results showed that these nanomaterials caused pulmonary inflammation and increased levels of C-reactive protein and serum amyloid A. The acute phase response induced by soluble and insoluble metal oxides was dose-dependent and had different time dependencies. The study also found that neutrophil influx, Saa3 mRNA levels in lung tissue, and plasma SAA3 levels were correlated across all studied nanomaterials, suggesting that these endpoints can be used as biomarkers of acute phase response and cardiovascular disease risk following exposure to soluble and insoluble particles.
PARTICLE AND FIBRE TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Adam Coln Hundahl, Arjen Weller, Jannik Bruun Larsen, Claudia U. Hj, Morten B. Hansen, Ann-Kathrin Muendler, Astrid Knuhtsen, Kasper Kristensen, Eva C. Arnspang, Thomas Lars Andresen, Kim I. Mortensen, Rodolphe Marie
Summary: Oral drug delivery is the preferred administration route for most drugs, but it is limited for biologics due to the intestinal barrier. One strategy to improve the absorption is chemical modification through lipidation. However, the mechanistic understanding of the effect is largely unexplored. This study developed a method to quantify peptide transport through a monolayer of Caco-2 cells and investigated the effects of lipidation on transport mechanism.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Anatomy & Morphology
Soren Bredmose Simonsen, Zhongtao Ma, Elisabeth Mariegaard, Salvatore De Angelis, Waynah Lou Dacayan, Kristian Speranza Molhave, Christodoulos Chatzichristodoulou
Summary: This study investigates the nanostructure and electrical conductivity of ion beam deposited Pt for operando electron microscopy at elevated temperatures. It reveals that the microstructure of Pt remains stable up to around 800 degrees C and with an applied current density of approximately 100 kA/cm(2). The conductivity increases with temperature due to densification, while changes in the hydrocarbon matrix have less influence. Recommendations are provided for optimizing Pt deposition parameters to maximize stability and minimize electrical resistance.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Chemistry, Physical
Zhongtao Ma, Waynah Lou Dacayan, Christodoulos Chatzichristodoulou, Kristian Speranza Molhave, Francesco Maria Chiabrera, Wenjing Zhang, Soren Bredmose Simonsen
Summary: The combination of electrical impedance spectroscopy (EIS) and environmental transmission electron microscopy (ETEM) is demonstrated by testing a specially designed micro gadolinia-doped ceria (CGO) sample. The EIS-TEM method provides valuable information on the structural and compositional aspects relevant to electrochemical performance. The developed platform is important for understanding nanoscale processes in green energy technologies.
Article
Chemistry, Physical
Rolf Erling Robberstad Moller-Nilsen, Silvia Canepa, Eric Jensen, Hongyu Sun, Ivan A. Moreno-Hernandez, Murat Nulati Yesibolati, A. Paul Alivisatos, Kristian S. Molhave
Summary: Liquid phase electron microscopy (LPEM) is important for studying chemical processes in situ, but the interaction between the liquid medium and the electron beam leads to radiolysis and the formation of reactive species that affect the studied processes. Current understanding of LPEM radiolysis is based on simulations using data collected at low electron flux intensities, requiring extrapolation to match the intensities used in LPEM. This study demonstrates a new approach to validate simulations by directly measuring radiolytic products during in situ LPEM.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Hjalte Rorbech Ambjorner, Anton Simon Bjornlund, Tobias Georg Bonczyk, Edwin Dollekamp, Lau Morten Kaas, Sofie Colding-Fagerholt, Kristian Speranza Molhave, Christian Danvad Damsgaard, Stig Helveg, Peter Christian Kjaergaard Vesborg
Summary: Graphene is the thinnest imaginable membrane with high impermeability, but leakage pathways at the graphene-substrate interface compromise its impermeability. This study provides a kinetic analysis of interface-mediated leakage and shows that thermal processing can significantly improve the leak rate.
Article
Biochemical Research Methods
Milan Finn Wesseler, Mathias Norbaek Johansen, Aysel Kiziltay, Kim Mortensen, Niels B. Larsen
Summary: This paper proposes a non-contact 3D mapping method for oxygen tension using commercially available oxygen microsensor beads embedded in confocal phosphorescence lifetime microscopy. The method accurately evaluates oxygen distribution in high cell-density cultures. Its versatility is demonstrated in the design and control of microperfused 3D printed hydrogel culture chips.
