Article
Biochemistry & Molecular Biology
Ziyan Zhang, Ping Xu, Zhe Hu, Zhaojian Fu, Tongyuan Deng, Xiaojie Deng, Lanzhu Peng, Yingying Xie, Lingzhi Long, Dayong Zheng, Peng Shen, Mengmin Zhang, Bin Gong, Zhibo Zhu, Junhao Lin, Rui Chen, Zhen Liu, Huilin Yang, Rong Li, Weiyi Fang
Summary: CCDC65 is a potential tumor suppressor in lung adenocarcinoma, which can suppress tumor development by inhibiting the cell cycle signal induced by c-Myc/ENO1.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Robert F. Shearer, Dimitris Typas, Fabian Coscia, Sofie Schovsbo, Thomas Kruse, Andreas Mund, Niels Mailand
Summary: Ubiquitin conjugation to substrate proteins regulates cellular processes and the K27 linkage type is essential for human cell proliferation. K27-linked ubiquitylation predominantly occurs in the nucleus and its disruption impairs nuclear ubiquitylation dynamics and cell cycle progression. K27-linked ubiquitylation supports cell fitness by facilitating p97-dependent processing of ubiquitylated nuclear proteins.
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Article
Biochemistry & Molecular Biology
Zhiyuan Zhang, Qi Wu, Meimiao Fang, Yu Liu, Ji Jiang, Qingyang Feng, Ronggui Hu, Jianmin Xu
Summary: In this study, it was found that HERC3 controls colorectal cancer (CRC) proliferation and the cell cycle by ubiquitination degradation of RPL23A, and further modulates cell proliferation through the c-Myc/p21 axis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yuda Huo, Wen Lu, Yuan Tian, Qingming Hou, Heng-Ye Man
Summary: This study found that Prkn knockout mice exhibit autistic-like behaviors and abnormalities in neuronal activity, cell morphology, and synapse density. This suggests the importance of the Prkn gene in brain development and the potential use of Prkn KO mice as a model for autism research.
Article
Biochemistry & Molecular Biology
Dong-Sik Chae, Sang Joon An, Seongho Han, Sung-Whan Kim
Summary: This study investigates the synergistic benefits of using dual 3D MSC culture systems to promote vascular regeneration and enhance therapeutic potential. The results demonstrate that dual 3D cultures of MSCs show higher expression of vascular growth factors and have better migration and tube formation abilities. In a mouse model, transplantation of dual 3D MSCs prevents limb loss and increases blood perfusion. The transplanted cells also show higher capillary density, angiogenic cytokine levels, and transdifferentiation into endothelial-like cells in the muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Julie Faitg, Clay Lacefield, Tracey Davey, Kathryn White, Ross Laws, Stylianos Kosmidis, Amy K. Reeve, Eric R. Kandel, Amy E. Vincent, Martin Picard
Summary: This study used quantitative three-dimensional electron microscopy to map mitochondrial network morphology and complexity in the mouse brain. Differences in mitochondrial morphology across different cell types and ages were identified, providing a foundation for further research on the role of neuronal mitochondria in the aging brain.
Article
Cell Biology
Shenghuan Sun, Justin Torok, Christopher Mezias, Daren Ma, Ashish Raj
Summary: This study utilizes machine learning to model the relationship between cell type composition and connectivity, demonstrating a link between cell types and connectivity in both long-range and short-range connections.
Article
Multidisciplinary Sciences
Miho Kawata, Yu Kodani, Mahito Ohkuma, Ei-ichi Miyachi, Yoko S. Kaneko, Akira Nakashima, Hidetaka Suga, Toshiki Kameyama, Kanako Saito, Hiroshi Nagasaki
Summary: Stem cell-derived hypothalamic neurons can be transplanted orthotopically into the mouse brain and exhibit axonal projections extending along hypothalamic nerve bundles, including projections into the posterior pituitary.
Article
Multidisciplinary Sciences
Sharon Kaisari, Shirly Miniowitz-Shemtov, Danielle Sitry-Shevah, Pnina Shomer, Guennadi Kozlov, Kalle Gehring, Avram Hershko
Summary: The mitotic checkpoint system ensures accurate chromosome segregation in mitosis. UBR5 is a protein associated with mitotic checkpoint proteins and plays a role in regulating the inactivation of the mitotic checkpoint.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Danielle Shevah-Sitry, Shirly Miniowitz-Shemtov, Adar Teichner, Sharon Kaisari, Avram Hershko
Summary: The ubiquitin ligase APC/C plays a crucial role in the control of mitosis. The phosphorylation of Cdc20 and its interaction with APC/C are important for its function in mitosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Meihui Chen, Rebecca W. Y. Chan, Peter P. H. Cheung, Meng Ni, Danny K. L. Wong, Ze Zhou, Mary-Jane L. Ma, Liangbo Huang, Xinzhou Xu, Wing-Shan Lee, Guangya Wang, Kathy O. Lui, W. K. Jacky Lam, Jeremy Y. C. Teoh, Chi-Fai Ng, Peiyong Jiang, K. C. Allen Chan, Rossa W. K. Chiu, Y. M. Dennis Lo
Summary: This study investigated the roles of DNASE1 and DNASE1L3 in the fragmentation of ucfDNA using mouse models and found DNASE1 to be an important nuclease in ucfDNA fragmentation. The study also evaluated the potential of ucfDNA fragmentation in bladder cancer detection through analyzing the concentrations and activities of DNASE1 in urine and plasma, as well as the ucfDNA fragment end data in bladder cancer patients.
Article
Materials Science, Multidisciplinary
Reza Moonesi Rad, Brandon Daul, Phillip Glass, Richard Joh, Charles Pemberton, Andy Shar, Jong Hyuk Kim, Daeha Joung
Summary: 3D printed platforms have versatile applications in cell-based assays and biomimetic tissue creation, investigating cell interactions and drug discovery. A fabrication strategy is proposed for magnetically controllable and shape-morphing 3D printed scaffolds. This transformative cell culture assessment using this platform could revolutionize 3D cell culture and advance personalized treatments.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Neurosciences
Rachael F. Ryner, Isabel D. Derera, Moritz Armbruster, Anar Kansara, Mary E. Sommer, Antonella Pirone, Farzad Noubary, Michele Jacob, Chris G. Dulla
Summary: Infantile and epileptic spasms syndrome (IESS) is characterized by spasms, abnormal EEG, and epilepsy. The molecular and circuit-level causes of IESS are not well understood. This study found that inhibitory circuit dysfunction occurs in mice with deletions in adenomatous polyposis coli (APC), which may contribute to IESS-related phenotypes.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Oksana Y. Dudaryeva, Aurelia Bucciarelli, Giovanni Bovone, Florian Huwyler, Shibashish Jaydev, Nicolas Broguiere, Marwa al-Bayati, Marco Lutolf, Mark W. Tibbitt
Summary: The biophysical properties of the cellular microenvironment, such as stiffness and geometry, have been shown to influence cell function, with recent findings implicating 3D confinement as a significant regulator of cell behavior. This study demonstrates that the extent of 3D confinement affects cell viability and proliferation, leading to changes in nuclear morphology and YES-associated protein (YAP) localization. The effects of confinement on cell behavior scale with the degree of 3D confinement, highlighting the importance of 3D confinement as a regulator of cell function.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell Biology
Jie Ran, Min Liu, Jie Feng, Haixia Li, Huixian Ma, Ting Song, Yu Cao, Peng Zhou, Yuhan Wu, Yunfan Yang, Yang Yang, Fan Yu, Heng Guo, Liang Zhang, Songbo Xie, Dengwen Li, Jinmin Gao, Xiaomin Zhang, Xueliang Zhu, Jun Zhou
DEVELOPMENTAL CELL
(2020)
Article
Cell Biology
Zhenguo Zhang, Songbo Xie, Ruoxi Wang, Shuqun Guo, Qiuchen Zhao, Hui Nie, Yuanyuan Liu, Fengguo Zhang, Miao Chen, Libo Liu, Xiaoqian Meng, Min Liu, Li Zhao, Monica P. Colaiacovo, Jun Zhou, Jinmin Gao
JOURNAL OF CELL BIOLOGY
(2020)
Article
Medicine, Research & Experimental
Rachel J. Perry, Kun Lyu, Aviva Rabin-Court, Jianying Dong, Xiruo Li, Yunfan Yang, Hua Qing, Andrew Wang, Xiaoyong Yang, Gerald Shulman
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Cell Biology
Yuanyuan Liu, Qiuchen Zhao, Hui Nie, Fengguo Zhang, Tingting Fu, Zhenguo Zhang, Feifei Qi, Ruoxi Wang, Jun Zhou, Jinmin Gao
Summary: The SYP-5 protein is identified as a critical regulator of meiotic thermotolerance, affecting crossover designation and bivalent formation. Homolog segregation errors become more severe at elevated temperature, with defects during the pachytene stage responsible for reduced viability in syp-5 mutants. These findings reveal a novel molecular mechanism for regulating meiotic thermotolerance.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Libo Liu, Jiaxiang Li, Yueshuang Ke, Xianlu Zeng, Jinmin Gao, Xueqing Ba, Ruoxi Wang
Summary: Parthanatos is a form of regulated cell death that plays a role in the pathogenesis of many diseases, particularly neurodegenerative disorders. Understanding the key factors and biochemical mechanisms of the Parthanatos pathway is essential for developing drugs and therapeutic strategies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Fengguo Zhang, Mengfei Liu, Jinmin Gao
Summary: Around 10% of reproductive-aged couples suffer from infertility, but the genetic causes of human infertility are largely unknown. Errors in meiosis, the process that produces gametes for fertilization, are associated with infertility in both males and females. Successful meiosis relies on the assembly of the synaptonemal complex (SC) between paired homologous chromosomes. Mutations in SC coding genes have been linked to human infertility, and complex interaction networks associated with SC components have been identified. Further investigations are needed to determine the role of genetic variations and environmental factors in human infertility.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jiaxiang Li, Jinmin Gao, Ruoxi Wang
Summary: Liquid-liquid phase separation plays a crucial role in various biological activities, being driven by interactions between multivalent and intrinsically disordered proteins. The organization of chromatin in a complex and small nucleus remains to be fully understood, with recent findings supporting the involvement of phase separation in regulating chromatin organization and chromosome behavior. Furthermore, phase separation also influences key events during mitosis and meiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Qian Wang, Dehai Li, Guangchao Cao, Qiping Shi, Jing Zhu, Mingyue Zhang, Hao Cheng, Qiong Wen, Hao Xu, Leqing Zhu, Hua Zhang, Rachel J. Perry, Olga Spadaro, Yunfan Yang, Shengqi He, Yong Chen, Baocheng Wang, Guangqiang Li, Zonghua Liu, Caixian Yang, Xiaoli Wu, Libing Zhou, Qinghua Zhou, Zhenyu Ju, Hongyun Lu, Yongjie Xin, Xiaoyong Yang, Cunchuan Wang, Yong Liu, Gerald I. Shulman, Vishwa Deep Dixit, Ligong Lu, Hengwen Yang, Richard A. Flavell, Zhinan Yin
Summary: Studies have shown that IL-27 plays a critical role in improving thermogenesis, countering obesity, and ameliorating insulin resistance, making it a promising target for anti-obesity immunotherapy.
Letter
Cell Biology
Huixian Ma, Feifei Qi, Li Ji, Songbo Xie, Jie Ran, Min Liu, Jinmin Gao, Jun Zhou, Xuebiao Yao
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ting Song, Yunfan Yang, Peng Zhou, Jie Ran, Liang Zhang, Xiaofan Wu, Wei Xie, Tao Zhong, Hongbin Liu, Min Liu, Dengwen Li, Huijie Zhao, Jun Zhou
Summary: In this study, ENKD1 was identified as a centrosomal protein involved in the regulation of ciliogenesis. The research demonstrated the critical role of ENKD1 in ciliogenesis. The study revealed the protein-protein interactions between ENKD1, CP110, and CEP97, as well as the mechanism of action of ENKD1 during ciliogenesis.
Review
Biochemistry & Molecular Biology
Yuanyuan Liu, Jinmin Gao
Summary: Reproductive aging is a natural process that occurs in both females and males, leading to fertility decline and detrimental effects on offspring. Factors such as oxidative stress, mitochondrial defects, telomere shortening, chromosome segregation errors, and genetic alterations contribute to reproductive aging. Understanding the biological pathways and implementing interventive strategies can help delay or rescue the aging phenotypes of reproduction.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Taicong Tan, Yingjin Tan, Ying Wang, Xiao Yang, Binyuan Zhai, Shuxian Zhang, Xuan Yang, Hui Nie, Jinmin Gao, Jun Zhou, Liangran Zhang, Shunxin Wang
Summary: This study reveals that negative supercoils play a crucial role in connecting interference and crossover interference in meiosis, and top2 gene mutations affect the accumulation and relief of negative supercoils, thereby regulating the strength of crossover interference.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Ting Song, Na He, Ziqian Hao, Yunfan Yang
Summary: This study found that the gene ENKD1 is highly expressed in DLBCL and is associated with the growth and progression of the disease. Inhibiting ENKD1 induces apoptosis, suppresses cell proliferation, and blocks cell cycle progression in the G2/M phase of DLBCL cells. Furthermore, ENKD1 expression is positively correlated with the expression levels of several cellular homeostatic regulators, including the gene Sperm-associated antigen 5, which encodes an important mitotic regulator. These findings suggest that ENKD1 plays a critical role in regulating cellular homeostasis and may be a potential target for DLBCL treatment.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Review
Andrology
Feng-Guo Zhang, Rui-Rui Zhang, Jin-Min Gao
Summary: The synaptonemal complex (SC) is a proteinaceous macromolecular structure that assembles between paired homologous chromosomes during meiosis in various eukaryotes, playing critical roles in accurate meiotic chromosome segregation. Recent studies using super-resolution microscopy have provided insights into the structure and regulation of the SC, shedding light on its biological functions.
ASIAN JOURNAL OF ANDROLOGY
(2021)
