Article
Multidisciplinary Sciences
Alice Meroni, Jan Grosser, Sumedha Agashe, Natasha Ramakrishnan, Jessica Jackson, Priyanka Verma, Laura Baranello, Alessandro Vindigni
Summary: Cells can adapt to multiple doses of TOP1 inhibitors by degrading TOP1-cc, which allows them to tolerate subsequent doses better. This adaptive response relies on the activation of the NEDD8-Cullin 3 pathway.
Review
Pharmacology & Pharmacy
Yifan Chen, Xuejing Shao, Ji Cao, Hong Zhu, Bo Yang, Qiaojun He, Meidan Ying
Summary: The crosstalk between phosphorylation and ubiquitination plays a crucial role in tumorigenesis, providing new directions and strategies for tumor therapy.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Biochemistry & Molecular Biology
Stephanie Diaz, Kankan Wang, Benita Sjogren, Xing Liu
Summary: Maintenance of protein homeostasis is crucial for eukaryotic biology, and the ubiquitin-proteasome system (UPS) plays an important role in the pathogenesis of cardiovascular diseases. Recent research has found that cullin-RING ubiquitin ligases (CRLs), an essential component of UPS, have significant physiological and pathological functions in the cardiovascular system.
Article
Developmental Biology
Aditi Dubey, Jianshi Yu, Tian Liu, Maureen A. Kane, Jean-Pierre Saint-Jeannet
Summary: In Xenopus embryos, Zic1 regulates PPR formation by activating RA production and promoting the expression of Cyp26c1 and Pitx2c to maintain low levels of RA in the vicinity of PPR. Disruption of these genes results in defective cranial placodes, suggesting their crucial roles in PPR specification.
Article
Multidisciplinary Sciences
Yeon-Woo Cho, Seohyeon Jee, Intan Rosalina Suhito, Jeong-Hyeon Lee, Chun Gwon Park, Kyung Min Choi, Tae-Hyung Kim
Summary: This study reports a new platform called SMENA, which enables efficient generation of neuronal cells using single metal-organic framework nanoparticles embedded in nanopit arrays. The platform allows for long-term storage and release of retinoic acid, leading to enhanced mRNA expressions of neurogenesis-related activities in neural stem cells.
Article
Biochemistry & Molecular Biology
Guangqiang Zhang, Junjiao Yang, Xiaoyu Zhao, Qinxue Li, Yunzhen Wu, Fangyuan Li, Yong Wang, Qunqun Hao, Wei Wang
Summary: Abscisic acid (ABA) is a key regulator of plant growth and stress responses. The E3 ligase TaPUB1 in wheat acts as a negative regulator of the ABA signaling pathway by interacting with TaPYL4 and TaABI5, affecting seed development. TaPUB1 expression levels in wheat influence sensitivity to ABA, seed size, and yield.
Article
Biotechnology & Applied Microbiology
Xiao-Li Zhang, Xiao-Qing Gong, Xin-Jian Su, Hai-Xia Yu, Si-Yuan Cheng, Jing-Wen Huang, Dan-Yang Li, Zhao-Long Lei, Ming-jun Li, Feng-wang Ma
Summary: In this study, it was found that MdRAD23D1 and MdPRP6 proteins in apple plants have opposite regulatory roles in drought stress. MdRAD23D1 levels increased under drought condition, accelerating the degradation of MdPRP6, while MdPRP6 negatively regulated drought response by regulating proline accumulation. Therefore, MdRAD23D1 and MdPRP6 together confer drought stress tolerance in apple plants.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Gastroenterology & Hepatology
Alessio Lepore, Pui Man Choy, Nathan C. W. Lee, Maria Annunziata Carella, Rosy Favicchio, Marco A. Briones-Orta, Shannon S. Glaser, Gianfranco Alpini, Clive D'Santos, Reuben M. Tooze, Mihaela Lorger, Wing-Kin Syn, Athanasios Papakyriakou, Georgios Giamas, Concetta Bubici, Salvatore Papa
Summary: The study found that activation of the JNK pathway promotes ICC cell proliferation by affecting the stability of PIN1, and highlights the JNK-PIN1 regulatory axis as an important factor in ICC growth.
Article
Plant Sciences
Yang Dong, Jing Liu, Liyuan Huang, Lina Yang, Muhammad Khalil-Ur-Rehman, Huan Zheng, Jianmin Tao
Summary: The VvSVP3 gene plays a crucial role in bud dormancy in grape by regulating ABA, GA, and callose synthesis. The study also identifies VvPYL9 as a target gene of VvSVP3, which activates the dormancy signaling pathway.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Han Lu, Youhong Fan, Yulin Yuan, Xiangli Niu, Bingyu Zhao, Yongsheng Liu, Fangming Xiao
Summary: In this study, the gene SlSTK in tomato was characterized and its role in glucose signaling was determined. SlSTK protein was localized in the nucleus and its expression was induced by glucose treatment. Overexpression of SlSTK enhanced glucose sensitivity in tomato, while knockout of SlSTK attenuated the sensitivity to glucose. Furthermore, SlSTK was found to be ubiquitinated and degraded by the ubiquitin ligase SlSINA4.
Article
Plant Sciences
Li Hua Cui, Hye Jo Min, Seong Guan Yu, Mi Young Byun, Tae Rin Oh, Andosung Lee, Hee Woong Yang, Woo Taek Kim
Summary: This study identified that OsATL38 negatively regulates cold stress response in rice by mono-ubiquitination of OsGF14d, while OsGF14d plays a positive role in response to cold stress.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agriculture, Multidisciplinary
Longmiao Zhang, Shuangbo Huang, Kaidi Ma, Yiling Chen, Tanghong Wei, Hongxuan Ye, Junyi Wu, Liudan Liu, Jinping Deng, Hefeng Luo, Chengquan Tan
Summary: This study found that Deoxynivalenol (DON) caused placental functional impairment and adverse pregnancy outcomes. Supplementation of 0.1% beta-carotene could alleviate the toxic effects of DON. The study also revealed the association of DON-induced placental function impairment with placental barrier disruption, angiogenesis impairment, and oxidative stress induction. Furthermore, beta-carotene regulated DON-induced placental toxicity through specific signaling pathways.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Michael C. Church, Andrew Price, Hua Li, Jerry L. Workman
Summary: This study uncovers a critical role of the remodeler Swi-Snf as a regulator of metabolism. The snf Delta mutants display a cysteine-deficient phenotype despite growing in nutrient-rich media, indicating perturbations in sulfur metabolic gene transcription. The findings demonstrate the importance of chromatin remodeling complexes in metabolic control.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Qi-Wen Wang, Ding-Jia-Cheng Jia, Jia-Min He, Yong Sun, Yun Qian, Qi-Wei Ge, Ya-Dong Qi, Qing-Yi Wang, Ying-Ying Hu, Lan Wang, Yan-Fei Fang, Hui-Qin He, Man Luo, Li-Jun Feng, Jian-Min Si, Zhang-Fa Song, Liang-Jing Wang, Shu-Jie Chen
Summary: L. intestinalis has a protective effect against ulcerative colitis by modulating the interaction between Th17 cells and gut epithelial cells. It inhibits Th17 cell differentiation by reducing the production of C/EBPA-driven SAA1 and SAA2, thus attenuating colitis progression.
Article
Biochemistry & Molecular Biology
Chenxi Ouyang, Guang Lu, Weifeng He, Boon-Huat Bay, Han-Ming Shen
Summary: The study reveals two forms of post-translational modifications of SIRT1 and their reciprocal effects. The post-translational modifications of SIRT1 play a crucial role in determining cell fate in response to DNA damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Eloi R. Verrier, Amelie Weiss, Charlotte Bach, Laura Heydmann, Vincent Turon-Lagot, Arnaud Kopp, Houssein El Saghire, Emilie Crouchet, Patrick Pessaux, Thomas Garcia, Patrick Pale, Mirjam B. Zeisel, Camille Sureau, Catherine Schuster, Laurent Brino, Thomas F. Baumert
Article
Biochemistry & Molecular Biology
Katerina Jerabkova, Yongrong Liao, Charlotte Kleiss, Sadek Fournane, Matej Durik, Arantxa Agote-Aran, Laurent Brino, Radislav Sedlacek, Izabela Sumara
Article
Biology
Shima Ghoroghi, Benjamin Mary, Annabel Larnicol, Nandini Asokan, Annick Klein, Nael Osmani, Ignacio Busnelli, Francois Delalande, Nicodeme Paul, Sebastien Halary, Frederic Gros, Laetitia Fouillen, Anne-Marie Haeberle, Cathy Royer, Coralie Spiegelhalter, Gwennan Andre-Gregoire, Vincent Mittelheisser, Alexandre Detappe, Kendelle Murphy, Paul Timpson, Raphael Carapito, Marcel Blot-Chabaud, Julie Gavard, Christine Carapito, Nicolas Vitale, Olivier Lefebvre, Jacky G. Goetz, Vincent Hyenne
Summary: This study identifies Ral GTPases as central molecules linking the mechanisms of EV secretion and cargo loading to their capacity to disseminate and induce pre-metastatic niches. Depletion of RalA or RalB in cells leads to limited organotropic capacities in vivo and less efficient promotion of metastasis, as well as reduced levels of the adhesion molecule MCAM/CD146 in EVs, which favors EV-mediated metastasis by targeting to the lungs. RalA, RalB, and MCAM/CD146 are factors of poor prognosis in breast cancer patients.
Article
Medicine, Research & Experimental
Devadarssen Murdamoothoo, Zhen Sun, Alev Yilmaz, Gilles Riegel, Cherine Abou-Faycal, Claire Deligne, Ines Velazquez-Quesada, William Erne, Marine Nascimento, Matthias Morgelin, Gerard Cremel, Nicodeme Paul, Raphael Carapito, Romain Veber, Helene Dumortier, Jingping Yuan, Kim S. Midwood, Thomas Loustau, Gertraud Orend
Summary: The study found that tenascin-C inhibits CD8 T cell infiltration by inducing CXCL12, which may contribute to the low response rates of immune checkpoint therapy in cancer treatment. Blocking the CXCR4 receptor of CXCL12 enhances macrophage and CD8 T cell infiltration, reducing tumor growth and metastasis.
EMBO MOLECULAR MEDICINE
(2021)
Article
Oncology
Cedric Schleiss, Raphael Carapito, Luc-Matthieu Fornecker, Leslie Muller, Nicodeme Paul, Ouria Tahar, Angelique Pichot, Manuela Tavian, Alina Nicolae, Laurent Miguet, Laurent Mauvieux, Raoul Herbrecht, Sarah Cianferani, Jean-Noel Freund, Christine Carapito, Myriam Maumy-Bertrand, Seiamak Bahram, Frederic Bertrand, Laurent Vallat
Summary: The study reveals a proliferative genetic program activated by BCR in primary leukemic cells, consisting of 430 genes and 374 proteins. Mathematical modeling highlights a transcriptional network associated with cell proliferation.
Article
Multidisciplinary Sciences
Tia A. Tummino, Veronica V. Rezelj, Benoit Fischer, Audrey Fischer, Matthew J. O'Meara, Blandine Monel, Thomas Vallet, Kris M. White, Ziyang Zhang, Assaf Alon, Heiko Schadt, Henry R. O'Donnell, Jiankun Lyu, Romel Rosales, Briana L. McGovern, Raveen Rathnasinghe, Sonia Jangra, Michael Schotsaert, Jean-Rene Galarneau, Nevan J. Krogan, Laszlo Urban, Kevan M. Shokat, Andrew C. Kruse, Adolfo Garcia-Sastre, Olivier Schwartz, Francesca Moretti, Marco Vignuzzi, Francois Pognan, Brian K. Shoichet
Summary: Phospholipidosis may be a shared mechanism underlying the antiviral activity of many repurposed drugs, and early detection and elimination of these effects can enable a focus on molecules with therapeutic potential.
Article
Multidisciplinary Sciences
Amit Kulkarni, Tiago Ferreira, Clemens Bretscher, Annabel Grewenig, Nazim El-Andaloussi, Serena Bonifati, Tiina Marttila, Valerie Palissot, Jubayer A. Hossain, Francisco Azuaje, Hrvoje Miletic, Lars A. R. Ystaas, Anna Golebiewska, Simone P. Niclou, Ralf Roeth, Beate Niesler, Amelie Weiss, Laurent Brino, Antonio Marchini
Summary: In-depth understanding of the life cycle of H-1 parvovirus (H-1PV) is crucial for the rational design of combination strategies and development of more effective viruses. The identification of laminin gamma 1 (LAMC1) as a crucial modulator of H-1PV infection highlights the importance of laminin family members in cell attachment and entry. Elevated levels of LAMC1 are found to correlate with increased H-1PV oncolytic activity in various cancer cell lines.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Gautier Follain, Nael Osmani, Valentin Gensbittel, Nandini Asokan, Annabel Larnicol, Luc Mercier, Maria Jesus Garcia-Leon, Ignacio Busnelli, Angelique Pichot, Nicodeme Paul, Raphael Carapito, Seiamak Bahram, Olivier Lefebvre, Jacky G. Goetz
Summary: The study found that blood flow characteristics are conducive to blocking tumor cell penetration and validated the importance of VEGFR in endothelial remodeling and subsequent tumor cell metastasis.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Caroline Spenle, Thomas Loustau, Helene Burckel, Gilles Riegel, Cherine Abou Faycal, Chengbei Li, Alev Yilmaz, Luciana Petti, Fanny Steinbach, Constance Ahowesso, Camille Jost, Nicodeme Paul, Raphael Carapito, Georges Noel, Fabienne Anjuere, Nathalie Salome, Gertraud Orend
Summary: The study using a novel syngeneic tongue OSCC grafting model found high TNC expression and TIL infiltration in irradiated tumors, while TNC knockout host showed less immune suppressive TME and tendency towards more tumor regression, which could be significant for future anti-cancer targeting of OSCC.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Myriam Lemmens, Benoit Fischer, Michael Zogg, Lindsey Rodrigues, Grainne Kerr, Alberto del Rio-Espinola, Fanny Schaeffer, Danilo Maddalo, Valerie Dubost, Alessandro Piaia, Arne Mueller, Ulla Plappert-Helbig, Ulrike Naumann, Jasmin Haegele, Alex Odermatt, Hans-Joerg Martus, Silvana Libertini
Summary: Off-target editing is a safety concern in CRISPR-Cas9 gene therapy, and two in vitro assays, SACF and GILA, provide sensitive and valid methods for evaluating tumorigenicity of genome-edited cells.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Immunology
Jeromine Klingler, Nicodeme Paul, Geraldine Laumond, Sylvie Schmidt, Luzia M. Mayr, Thomas Decoville, Olivier Lambotte, Brigitte Autran, Seiamak Bahram, Christiane Moog
Summary: The study found that HIV-1 controllers have a unique HIV-specific antibody profile that may contribute to the control of HIV replication. This emphasizes the importance of analyzing multifunctional antibody responses in vaccine research.
Article
Cell Biology
Raphael Carapito, Richard Li, Julie Helms, Christine Carapito, Sharvari Gujja, Veronique Rolli, Raony Guimaraes, Jose Malagon-Lopez, Perrine Spinnhirny, Alexandre Lederle, Razieh Mohseninia, Aurelie Hirschler, Leslie Muller, Paul Bastard, Adrian Gervais, Qian Zhang, Francois Danion, Yvon Ruch, Maleka Schenck, Olivier Collange, Thien-Nga Chamaraux-Tran, Anne Molitor, Angelique Pichot, Alice Bernard, Ouria Tahar, Sabrina Bibi-Triki, Haiguo Wu, Nicodeme Paul, Sylvain Mayeur, Annabel Larnicol, Geraldine Laumond, Julia Frappier, Sylvie Schmidt, Antoine Hanauer, Cecile Macquin, Tristan Stemmelen, Michael Simons, Xavier Mariette, Olivier Hermine, Samira Fafi-Kremer, Bernard Goichot, Bernard Drenou, Khaldoun Kuteifan, Julien Pottecher, Paul-Michel Mertes, Shweta Kailasan, M. Javad Aman, Elisa Pin, Peter Nilsson, Anne Thomas, Alain Viari, Damien Sanlaville, Francis Schneider, Jean Sibilia, Pierre-Louis Tharaux, Jean-Laurent Casanova, Yves Hansmann, Daniel Lidar, Mirjana Radosavljevic, Jeffrey R. Gulcher, Ferhat Meziani, Christiane Moog, Thomas W. Chittenden, Seiamak Bahram
Summary: In this study, multi-omics analysis and artificial intelligence were used to examine the differences in biological factors between critical and non-critical COVID-19 patients in a young and otherwise healthy cohort. The study identified ADAM9 as a driver of disease severity and a potential therapeutic target.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Aurore Vuidel, Loic Cousin, Beatrice Weykopf, Simone Haupt, Zahra Hanifehlou, Nicolas Wiest-Daessle, Michaela Segschneider, Joohyun Lee, Yong-Jun Kwon, Michael Peitz, Arnaud Ogier, Laurent Brino, Oliver Bruestle, Peter Sommer, Johannes H. Wilbertz
Summary: Combining multiple cellular phenotypes can improve the accuracy of midbrain dopaminergic neuron models for Parkinson's disease. Machine learning classification can detect chemical compound treatments related to LRRK2.
Article
Pathology
Enrica Saponara, Carlos Penno, Vanessa Orsini, Zhong-Yi Wang, Audrey Fischer, Alexandra Aebi, Meztli L. Matadamas-Guzman, Virginie Brun, Benoit Fischer, Margaret Brousseau, Peter O'Donnell, Jonathan Turner, Alexandra Graff Meyer, Laura Bollepalli, Giovanni dArio, Guglielmo Roma, Walter Carbone, Stefano Annunziato, Michael Obrecht, Nicolau Beckmann, Chandra Saravanan, Arnaud Osmont, Philipp Tropberger, Shola M. Richards, Christel Genoud, Svenja Ley, Iwona Ksiazek, Florian Nigsch, Luigi M. Terracciano, Heiko S. Schadt, Tewis Bouwmeester, Jan S. Tchorz, Heinz Ruffner
Summary: This study investigated the role of Lgr4/5 in nonalcoholic fatty liver disease (NAFLD) and found that the loss of Lgr4/5 in hepatic epithelial cells led to hepatic steatosis and fibrosis in mice fed with a high-fat diet. The study also demonstrated that the loss of Lgr4/5 resulted in impaired bile acid secretion, altered lipid homeostasis, and deregulation of lipoprotein pathways, all of which contributed to the onset of NAFLD.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Gastroenterology & Hepatology
Eloi R. Verrier, Gaetan Ligat, Laura Heydmann, Katharina Doernbrack, Julija Miller, Anne Maglott-Roth, Frank Juehling, Houssein El Saghire, Margaux J. Heuschkel, Naoto Fujiwara, Sen-Yung Hsieh, Yujin Hoshida, David E. Root, Emanuele Felli, Patrick Pessaux, Atish Mukherji, Laurent Mailly, Catherine Schuster, Laurent Brino, Michael Nassal, Thomas F. Baumert
Summary: Chronic hepatitis B virus (HBV) infection is a major cause of liver disease and hepatocellular carcinoma. The covalently closed circular (ccc) DNA, which is not targeted by current treatments, is crucial for viral cure. This study established a cell-based HBV cccDNA reporter assay and identified Y box binding protein 1 (YBX1) as an important host factor in the early steps of HBV life cycle. The expression of YBX1 in patients also correlates with HBV load and liver disease progression.