Article
Microbiology
Krupa Parmar, Lauren Komarow, Damon W. Ellison, Andrey A. Filippov, Mikeljon P. Nikolich, Joseph R. Fackler, Martin Lee, Anjna Nair, Priyesh Agrawal, Pranita D. Tamma, Maria Souli, Scott R. Evans, Kerryl E. Greenwood-Quaintance, Scott A. Cunningham, Robin Patel
Summary: Standardized approaches to phage susceptibility testing are crucial for selecting phages for bacterial infection studies. This study compared the results of phage susceptibility testing performed at different centers using different assay methods. The findings suggest that further development is needed to improve the reproducibility of phage susceptibility testing methods.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Seyyed Mehdy Elahi, Nazila Nazemi-Moghaddam, Christine Gadoury, Julie Lippens, Stevo Radinovic, Marie-Helene Venne, Anne Marcil, Renald Gilbert
Summary: The FFA method for titrating AdV using new monoclonal antibodies and automatic counting of positive cells is more precise and reliable compared to traditional methods.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Alessandra Pratt, Nile Eckermann, Shankar Rengasamy Venugopalan, Lina Moreno Uribe, Lauren Barlow, Matthew Nonnenmann
Summary: The aim of this study was to quantify the aerosol concentrations of bacteriophage MS2 near the oral cavity and behind the dental practitioner's face shield during a simulated orthodontic debanding procedure. The results showed that debanding generates live virus aerosol and face shields do not provide significant protection from virus aerosol, but they do offer some protection against splatter during the procedure.
SCIENTIFIC REPORTS
(2023)
Article
Clinical Neurology
Yilong Zheng, Mervyn Jun Rui Lim, Benjamin Yong-Qiang Tan, Bernard Poon Lap Chan, Prakash Paliwal, Ong Jia Yuan Jonathan, Chandra Bharatendu, Amanda Chee Yun Chan, Leonard Leong Litt Yeo, Joy Vijayan, Chiew S. S. Hong, Young Heng Chee, Lily Y. H. Wong, Jintao Chen, Victor Yao Feng Chong, Yanhong Dong, Chi Hsien Tan, Sibi Sunny, Hock Luen Teoh, Arvind Kumar Sinha, Vijay Kumar Sharma
Summary: This study aimed to validate the associations of plaque inflammation on FDG-PET and the symptomatic carotid atheroma inflammation lumen-stenosis (SCAIL) score with recurrent ischemic events. A single-center prospective cohort study recruited patients with recent TIA or ischemic stroke, carotid artery stenosis, and not considered for early revascularization. Measurements of plaque inflammation and stenosis severity were taken, and the results showed that FDG-PET demonstrated better predictive abilities than the SCAIL score in risk stratification for patients. The study concluded that further research is needed to evaluate the benefits of carotid revascularization and/or anti-inflammatory therapy based on plaque inflammation detected on FDG-PET.
FRONTIERS IN NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Vidyasagar Koduri, Leslie Duplaquet, Benjamin L. Lampson, Adam C. Wang, Amin H. Sabet, Mette Ishoey, Joshiawa Paulk, Mingxing Teng, Isaac S. Harris, Jennifer E. Endress, Xiaoxi Liu, Ethan Dasilva, Joao A. Paulo, Kimberly J. Briggs, John G. Doench, Christopher J. Ott, Tinghu Zhang, Katherine A. Donovan, Eric S. Fischer, Steven P. Gygi, Nathanael S. Gray, James Bradner, Jeffrey A. Medin, Sara J. Buhrlage, Matthew G. Oser, William G. Kaelin
Summary: Some undruggable proteins can be targeted by compounds that degrade them. Current assays for identifying degraders have limitations, but a new gain of signal assay has been developed to facilitate the discovery of drugs that degrade undruggable proteins directly or indirectly.
Article
Biochemistry & Molecular Biology
Marta Pla-Diaz, Leonor Sanchez-Buso, Lorenzo Giacani, David Smajs, Philipp P. Bosshard, Homayoun C. Bagheri, Verena J. Schuenemann, Kay Nieselt, Natasha Arora, Fernando Gonzalez-Candelas
Summary: The incidence of syphilis is increasing globally, and recombination and selection among the Treponema pallidum subspecies play an important role in the evolution and spread of the bacteria causing syphilis.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Food Science & Technology
Mo Jia, Jonathan D. Joyce, Andrea S. Bertke
Summary: SARS-CoV-2 can survive in common beverages, with minimal reductions in some types such as milk and beer, while others like cocoa, coffee, tea, fruit juices, and wine contain antiviral compounds that can inactivate the virus. Hard liquors containing 40% alcohol can immediately inactivate SARS-CoV-2, but mixing them with non-alcoholic beverages reduces the antiviral effects. This study suggests that beverages contaminated with SARS-CoV-2 could potentially be a vehicle for virus transmission.
Article
Plant Sciences
Yi-Ye Liang, Yong Shi, Shuai Yuan, Biao-Feng Zhou, Xue-Yan Chen, Qing-Qing An, Par K. Ingvarsson, Christophe Plomion, Baosheng Wang
Summary: Natural selection plays a key role in shaping genetic diversity within and between species, with selective sweeps and background selection influencing genomic variation. Results from studying oak species genomes show the impact of different types of selection models and the importance of linked selection in shaping genetic diversity.
Article
Computer Science, Artificial Intelligence
Wu Lin, Qiuzhen Lin, Junkai Ji, Zexuan Zhu, Carlos A. Coello Coello, Ka-Chun Wong
Summary: This paper proposes a novel bicriteria assisted adaptive operator selection strategy for decomposition-based multiobjective evolutionary algorithms. By using two operator pools focusing on exploitation and exploration, and two criteria emphasizing convergence and diversity, a good balance between exploitation and exploration during evolutionary search can be achieved. The experimental results show that the proposed B-AOS outperforms existing state-of-the-art adaptive operator selection methods and can significantly improve performance on benchmark problems.
SWARM AND EVOLUTIONARY COMPUTATION
(2021)
Article
Engineering, Biomedical
Tairan Liu, Yuzhu Li, Hatice Ceylan Koydemir, Yijie Zhang, Ethan Yang, Merve Eryilmaz, Hongda Wang, Jingxi Li, Bijie Bai, Guangdong Ma, Aydogan Ozcan
Summary: An automated plaque assay leveraging lens-free holographic imaging and deep learning rapidly and accurately detects the cell-lysing events caused by viral replication.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Wei Gao, Chuan-Xin Yu, Wei-Wei Zhou, Bao-Lin Zhang, E. Anne Chambers, Hollis A. Dahn, Jie-Qiong Jin, Robert W. Murphy, Ya-Ping Zhang, Jing Che
Summary: Speciation plays a crucial role in evolution, and understanding the origins and persistence of reproductive isolation (RI) is important. This study examined two closely related toad-headed lizards using genomic data and found that divergent selection and low genetic recombination help maintain RI between the two species.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Chemistry, Analytical
Jing Xu, Yinbing Liu, Ke-Jing Huang, Yang-Yang Hou, Xiaoxuan Sun, Jiaqiang Li
Summary: Realization of a highly sensitive analysis and sensing platform is crucial for early-stage tumor diagnosis. In this study, a self-powered biosensor with a novel sandwich graphdiyne (SGDY) structure combined with an aptamer-specific recognition function was developed to sensitively and accurately detect tumor markers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Alvaro Luque-Uria, Riikka Peltomaa, Tarja K. Nevanen, Henri O. Arola, Kristiina Iljin, Elena Benito-Pena, Maria C. Moreno-Bondi
Summary: In this study, cyclic peptides that mimic the behavior of the immunosuppressant drug mycophenolic acid (MPA) were developed using a phage-displayed peptide library. A recombinant fusion protein containing the peptide sequence and a bioluminescent enzyme, NanoLuc, was created for use as a tracer in competitive immunoassays. The bioluminescent sensor showed good selectivity towards MPA, with a detection limit of 0.26 ng mL(-1) and an IC50 of 2.9 +/- 0.5 ng mL(-1), and was successfully applied to the analysis of clinical samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Osama A. Abdalla, Akhtar Ali
Summary: This study collected 57 isolates of Watermelon mosaic virus (WMV) from cucurbit fields in nine southern states, and found high sequence identities among them. Phylogenetic analysis revealed that all U.S. WMV isolates belong to Group 3, with two clusters similar to previously reported subgroups and a new distinct subgroup formed in this study. Analysis showed low non-synonymous to synonymous nucleotide substitution ratio, indicating negative purifying selection in the CP gene of WMV.
Article
Ecology
Christopher R. Cooney, Judith E. Mank, Alison E. Wright
Summary: Research shows that sex differences in recombination rates are evolutionarily labile and not driven by simple neutral processes, contradicting nonadaptive explanations. While there is potential for adaptive processes in the patterns of heterochiasmy, the direct link to sexual selection or sexual conflict across species is unclear, indicating subtle or complex effects. Additionally, evidence suggests correlated rates of recombination rate evolution between males and females, suggesting genetic constraints and sexual conflict over the recombination landscape.
Article
Immunology
Tracy H. Hazen, Susan R. Leonard, Keith A. Lampel, David W. Lacher, Anthony T. Maurelli, David A. Rasko
INFECTION AND IMMUNITY
(2016)
Article
Microbiology
George Liechti, Erkin Kuru, Mathanraj Packiam, Yen-Pang Hsu, Srinivas Tekkam, Edward Hall, Jonathan T. Rittichier, Michael VanNieuwenhze, Yves V. Brun, Anthony T. Maurelli
Article
Microbiology
George Liechti, Raghuveer Singh, Patricia L. Rossi, Miranda D. Gray, Nancy E. Adams, Anthony T. Maurelli
Article
Infectious Diseases
Marta Fogolari, Carla Mavian, Silvia Angeletti, Marco Salemi, Keith A. Lampel, Anthony T. Maurelli
INFECTION GENETICS AND EVOLUTION
(2018)
Article
Microbiology
Jessica A. Slade, Mary Brockett, Raghuveer Singh, George W. Liechti, Anthony T. Maurelli
Article
Immunology
Raghuveer Singh, George Liechti, Jessica A. Slade, Anthony T. Maurelli
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Dev K. Ranjit, George W. Liechti, Anthony T. Maurelli
Article
Public, Environmental & Occupational Health
Sarah L. McKune, Daniel Acosta, Nick Diaz, Kaitlin Brittain, Diana Joyce-Beaulieu, Anthony T. Maurelli, Eric J. Nelson
Summary: This study conducted a cross-sectional study among K-12 students in April 2020 and found that loss of household income, being female, parental COVID-19 protective practices, and lower school level were associated with increased risk of anxiety, depressive and OCD-related symptoms in children and adolescents during the COVID-19 pandemic.
Article
Environmental Sciences
Daniel Acosta, Yui Fujii, Diana Joyce-Beaulieu, K. D. Jacobs, Anthony T. Maurelli, Eric J. Nelson, Sarah L. McKune
Summary: The study found no significant difference in anxiety, depression, and OCD risk levels between students engaged in Emergency Remote Education (ERE) and in-person learning. Participation in sports was identified as a protective factor against the risk of anxiety, depression, and OCD.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Gastroenterology & Hepatology
Dennis Yang, Yaseen B. Perbtani, Julia Loeb, Nanlong Liu, Peter Draganov, David E. Estores, Michael Lauzardo, Anthony Maurelli, John A. Lednicky, J. Glenn Morris
Summary: This study aimed to determine the presence of SARS-CoV-2 in the gastrointestinal tracts of patients scheduled for endoscopy, with results showing rare detection of the virus in these patients with negative nasopharyngeal testing. Additionally, no live virus was detected in any of the collected specimens after a 14-day incubation period.
ENDOSCOPY INTERNATIONAL OPEN
(2021)
Review
Microbiology
Melissa Mae Lamanna, Anthony T. Maurelli
Summary: Understanding how proteins move in cells is crucial in biology. This review focuses on the motion of peptidoglycan (PG) synthesis machines in bacteria. By examining several bacterial systems, we gain a comprehensive understanding of their movement dynamics. Recent imaging techniques reveal that these machines are not static but exhibit directed motion in rod-shaped bacteria like E. coli and B.subtilis. The enzymatic activity and treadmilling of certain proteins drive this motion.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Environmental Sciences
Andrew L. Rainey, Julia C. Loeb, Sarah E. Robinson, John A. Lednicky, John McPherson, Sue Colson, Michael Allen, Eric S. Coker, Tara Sabo-Attwood, Anthony T. Maurelli, Joseph H. Bisesi
Summary: This study uses wastewater measurements of SARS-CoV-2 to monitor viral trends and variants in a small island community, and evaluates the influence of tourism on viral loads. The results show a positive association between the number of visitors and the concentration of SARS-CoV-2 RNA in wastewater, and the detection of a new variant. The study demonstrates the utility of wastewater surveillance in small communities for monitoring SARS-CoV-2.
ENVIRONMENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Andrew Rainey, Song T. Liang, Joseph H. Bisesi Jr, Tara Sabo-Attwood, Anthony Maurelli
Summary: Flow normalized SARS-CoV-2 wastewater concentrations show the strongest correlation with COVID-19 cases, while human fecal indicators have lower correlation. Utilizing reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) significantly improves correlation values.
Article
Immunology
Raghuveer Singh, Jessica A. Slade, Mary Brockett, Daniel Mendez, George W. Liechti, Anthony T. Maurelli
Summary: The study demonstrates the functionality of DapF in Chlamydia trachomatis through a substrate competition strategy, showing that excess substrate can inhibit specific enzyme activities. The findings suggest that suppressing either the glutamate racemase or epimerase activity of DapF compromises the growth of the bacterium.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Kimberly A. Bliven, Anthony T. Maurelli
MICROBIOLOGY SPECTRUM
(2016)