Article
Multidisciplinary Sciences
Juliana C. Correa-Velloso, Paula J. Bartlett, Robert Brumer, Lawrence D. Gaspers, Henning Ulrich, Andrew P. Thomas
Summary: Rat hepatocytes express multiple purinergic P2Y and P2X receptors, leading to distinct Ca2+ oscillations. These oscillation patterns are shaped by protein kinase C, resulting in unique cellular responses to different agonists.
Article
Environmental Sciences
Rui-Ting Wu, Jing-Yuan Chen, Shuo Liu, Shi-Hua Niu, Xin-Di Liao, Si-Cheng Xing
Summary: The study showed that microplastics combined with antibiotics can stimulate the metabolic cooperation of bacteria to promote proliferation, especially in the presence of high concentrations of microplastics. Microplastics slowed down the growth speed of Escherichia coli, enhanced that of Pseudomonas aeruginosa, and notably improved the latter's biofilm formation ability.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Crystian Massengill, Landon Bayless-Edwards, Cesar C. Ceballos, Elizabeth R. Cebul, James Cahill, Arpita Bharadwaj, Evan Wilson, Maozhen Qin, Matthew R. Whorton, Isabelle Baconguis, Bing Ye, Tianyi Mao, Haining Zhong
Summary: cAMPFIREs are genetically encoded cAMP sensors suitable for in vivo imaging of cAMP signaling. They exhibit high sensitivity and cell specificity, showing good performance in in vivo imaging.
Article
Multidisciplinary Sciences
Sonia Colombo, Maddalena Collini, Laura D'Alfonso, Giuseppe Chirico, Enzo Martegani
Summary: This study reveals that the Ras/cAMP/PKA pathway plays a crucial role in regulating the response to nutrients' availability and stress in budding yeast. The activity of this pathway leads to noisy oscillations of cAMP levels, which in turn affects the nuclear accumulation of the transcription factor Msn2. Using a sensor, the researchers were able to detect the changes in cAMP concentrations in single yeast cells and observed oscillations under conditions of nutritional stress caused by limiting glucose availability.
Article
Cell Biology
Hyunjoo Kim, Muhah Jeong, Do-Hyeong Na, Shin-Hyeon Ryu, Eun Il Jeong, Kwangmin Jung, Jaemin Kang, Ho-June Lee, Taebo Sim, Dae-Yeul Yu, Hee Chul Yu, Baik-Hwan Cho, Yong-Keun Jung
Summary: The study reveals that AK2 functions as a suppressor of BRAF, regulating its activity in response to cellular metabolic state. AK2 interacts with BRAF and inhibits its activity and downstream ERK phosphorylation. The research also finds that low expression of AK2 and increased ERK activation are associated with hepatocellular carcinoma (HCC), and loss of AK2 promotes tumor growth and BRAF activity.
CELL DEATH & DISEASE
(2022)
Article
Biology
Timothy W. Church, Parul Tewatia, Saad Hannan, Joao Antunes, Olivia Eriksson, Trevor G. Smart, Jeanette Hellgren Kotaleski, Matthew G. Gold
Summary: The study found that AKAP79 enhances calcineurin activity, which leads to the suppression of PKA without altering cAMP levels. This non-canonical mode of PKA regulation may play a role in many other cellular processes.
Article
Urology & Nephrology
Xiaofang Wang, Li Jiang, Ka Thao, Caroline R. Sussman, Timothy LaBranche, Michael Palmer, Peter C. Harris, G. Stanley McKnight, Klaus P. Hoeflich, Stefanie Schalm, Vicente E. Torres
Summary: Upregulation of cAMP-dependent and cAMP-independent PKA signaling promotes cystogenesis in polycystic kidney disease (PKD). Activation of PKA-I promotes cystogenesis, while its inhibition prevents cyst formation. Inhibition of PKA activity can directly and/or indirectly inhibit multiple pro-proliferative pathways and is protective in vivo.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Pharmacology & Pharmacy
Anastasios Lymperopoulos
Summary: This article discusses the research status of cAMP levels in heart diseases, and finds that there is currently no consensus on the levels of cAMP in human failing hearts. However, many studies indicate that cAMP levels are deficient in human failing hearts, suggesting that strategies to increase rather than decrease these levels should be pursued.
EXPERT REVIEW OF CLINICAL PHARMACOLOGY
(2023)
Article
Urology & Nephrology
Stephen C. Parnell, Archana Raman, Yan Zhang, Emily A. Daniel, Yuqiao Dai, Aditi Khanna, Gail A. Reif, Jay L. Vivian, Timothy A. Fields, Darren P. Wallace
Summary: This study demonstrates that active BRAF is sufficient to induce kidney cyst formation in normal mice and accelerate cystic disease in PKD mice.
KIDNEY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Wenli Yang, William G. Robichaux, Fang C. Mei, Wei Lin, Li Li, Sheng Pan, Mark A. White, Yuan Chen, Xiaodong Cheng
Summary: It has been found that cAMP regulates cellular SUMOylation through Epac1, which also controls the dynamics of cellular condensates. This research provides insights into the molecular mechanism linking cellular stress responses and protein SUMOylation.
Review
Biochemistry & Molecular Biology
Helen R. Saibil
Summary: This article discusses the crucial role played by PDB in the development of molecular chaperones and protein homeostasis, focusing on key structures that demonstrate how chaperones assist in specific folding pathways and provide mechanistic insights into ATPase machinery. Additionally, it highlights the framework provided by PDB for managing and distributing structural biology data.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Stephanie S. Kim, Ina Kycia, Michael Karski, Rosanna K. Ma, Evan A. Bordt, Julian Kwan, Anju Karki, Elle Winter, Ranan G. Aktas, Yuxuan Wu, Andrew Emili, Daniel E. Bauer, Praveen Sethupathy, Khashayar Vakili
Summary: Fibrolamellar carcinoma (FLC) is a primary liver cancer with a poor prognosis. The study engineered the DP fusion gene in HEK293T cells to investigate its cellular effects. The results showed alterations in mitochondrial dynamics and increased LINC00473 expression in HEK-DP cells, suggesting potential roles of DP in mitochondrial dynamics and LINC00473 activity.
Article
Ophthalmology
Angela Garriz, Junji Morokuma, Danny Toribio, Driss Zoukhri
Summary: The involvement of the second messenger 3',5'-cyclic adenosine monophosphate (cAMP) and its downstream effectors in oxytocin (OXT)-mediated lacrimal gland myoepithelial cell (MEC) contraction was investigated. The expression of adenylate cyclase coupling G proteins, Gαs, Gαo, and Gαi, was observed in lacrimal gland MEC. OXT increased intracellular cAMP levels and cAMP agonists stimulated MEC contraction via PKA and EPAC activation, playing a major role in OXT-induced MEC contraction.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Heinrich K. Wasner
Summary: Metformin is the main drug for treating type 2 diabetes, and its mechanism of action, although largely unknown, includes the activation of AMP-kinase, reduction of blood glucose levels, inhibition of cyclic AMP synthesis, gluconeogenesis, and increased sensitivity to insulin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jared D. Chrispell, Yubin Xiong, Ellen R. Weiss
Summary: This study investigates the role of cAMP-dependent GRK phosphorylation in cone photoreceptor recovery. Experiment results in zebrafish show that elevated cAMP levels significantly decrease the recovery of cone photoresponse, mediated by Grk7a instead of Grk1b. Using a cone-specific dominant negative PKA transgene, it is demonstrated for the first time that PKA is required for the phosphorylation of Grk7a in vivo. Furthermore, immunoblot analyses reveal that cone-expressed Grk1 does not undergo cAMP-dependent phosphorylation in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)