Article
Genetics & Heredity
C. Isabel Wagner, Martina E. L. Kopp, James Thorburn, Catherine S. Jones, Galice Hoarau, Leslie R. Noble
Summary: In this study, the nuclear genome of the spiny dogfish, a shark of interest to biomedical and conservation efforts, was sequenced and annotated. The genome assembly has a size of 3.7 Gb and a completeness score of 91.6%. A total of 33,283 gene models were predicted, with 31,979 functionally annotated.
G3-GENES GENOMES GENETICS
(2023)
Article
Biology
J. Lisa Hoogenboom, W. Gary Anderson
Summary: Marine elasmobranchs retain large concentrations of urea to balance osmotic pressure and take in exogenous nitrogen to maintain nitrogen balance and satisfy osmoregulatory and somatic processes. Dietary nitrogen is directed towards the synthesis of specific nitrogenous molecules, with preferential accumulation and retention of nitrogen for osmoregulatory purposes.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christopher P. Cutler, Keith Kurt, Karen E. Campbell, Tolulope Ojo
Summary: Immunohistochemical localization of three aquaporin water channel proteins (AQP3, AQP4, and AQP15) in the kidney of the spiny dogfish revealed that AQP4/2 and AQP15 are primarily located in the late distal tubule loop, while AQP3 is mainly located at the end of the late distal tubule loop. The strong staining of AQP4/2, AQP15, and Na+,K+-ATPase antibodies was found at the beginning of the late distal tubule loop.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Fisheries
Romana Gracan, Bojan Lazar, Sara Zupan, Elena Buzan
Summary: The study assessed the genetic diversity of the spiny dogfish population in the Mediterranean Sea and highlighted its endangered status. The results indicate a unique genetic structure of spiny dogfish in the Adriatic Sea, suggesting the need for separate management and conservation efforts for this regionally exploited and endangered shark species.
MARINE AND FRESHWATER RESEARCH
(2021)
Article
Biology
Mathias Schakmann, Victoria Becker, Mathias Sogaard, Jacob L. Johansen, John F. Steffensen, Paolo Domenici
Summary: Fast escape responses are crucial for the survival of marine organisms, but elasmobranchs are often overlooked in studies. In this research, the escape latency of the Pacific spiny dogfish was measured and compared with two teleosts, revealing that the dogfish had a longer latency possibly due to the absence of Mauthner cells.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Physiology
J. Lisa Hoogenboom, W. Gary Anderson
Summary: We investigated the mechanisms of nitrogen trafficking in North Pacific spiny dogfish and found that increased ammonia concentration did not stimulate the uptake of total urea or total methylamine. Feeding increased Rhp2 mRNA levels in the kidney and mid spiral valve region, while excess urea and antibiotics reduced Rhbg mRNA levels along all three spiral valve regions.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2023)
Article
Ecology
Mikael Ovegard, Johanna Hogvall, Maria Ovegard, Andreas Wikstrom, Hakan Wennhage
Summary: This study revealed an association between a teleost and a shark, and emphasized the importance of using stereo-BRUV to gain ecological knowledge and make observations about species associations and behaviors.
ENVIRONMENTAL BIOLOGY OF FISHES
(2022)
Article
Zoology
Sarah Arnette, Jacob Saffarian, Lara Ferry, Stacy Farina
Summary: The mechanics of ventilation in elasmobranchs involve a two-pump system dependent on differential pressures between the orobranchial and parabranchial cavities. However, the variation in parabranchial form and function is often overlooked. This study collected parabranchial pressures from Pacific spiny dogfish and found a posterior attenuation of pressure amplitudes, as well as differential use of parabranchial chamber five, suggesting distinct ventilatory modes and proposing a new model for ventilation in this species.
Article
Biochemistry & Molecular Biology
Christopher P. Cutler, Debra Murray, Tolulope Ojo, Sheena Harmon, Bryce MacIver, Gordon Cramb, Mark L. Zeidel
Summary: cDNAs for aquaporin genes AQP3 and AQP15 were cloned and sequenced to initiate the study. Northern blot analysis confirmed mRNA sizes, showing significant decrease in AQP15 expression with higher salinity. Microinjection of full length cDNAs into Xenopus oocytes increased water permeability, particularly in the presence of mercury and at neutral pH. Antibody staining and localization studies in fish tissues revealed specific membrane distributions of AQP3 and AQP15, suggesting a potential water absorption pathway in the intestine.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Ecology
Feriha Tserkova, Vesselina Mihneva, Elitsa Pavlova, Philip Penchev
Summary: Data on seasonal and interannual variations in the biological parameters of picked dogfish in the Black Sea have been limited, hindering the understanding of the current status and management options of this vulnerable species. This study examined the size and sex structure of picked dogfish in the Western Black Sea, as well as the link between length and weight, to identify the variability of biological parameters.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jess MacPherson, Alyssa M. Weinrauch, W. Gary Anderson, Carol Bucking
Summary: The gut microbiome plays a crucial role in nitrogen handling in fed spiny dogfish, as antibiotic treatment reduced microbial enzyme activity and resulted in lower plasma urea levels. This study suggests that compromising the gut microbiome significantly influences post-prandial nitrogen handling in these fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jess MacPherson, Alyssa M. Weinrauch, W. Gary Anderson, Carol Bucking
Summary: Nitrogen recycling through the gut microbiome is an important mechanism to reclaim nitrogen trapped in urea. This study found that compromising the gut microbiome significantly influences the nitrogen handling in spiny dogfish, and the recycling of urea-nitrogen is vital for maintaining nitrogen balance in these fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shiyong Yang, Datian Li, Langkun Feng, Chaoyang Zhang, Dandan Xi, Hongli Liu, Chaozhan Yan, Zihan Xu, Yujie Zhang, Yunkun Li, Taiming Yan, Zhi He, Jiayun Wu, Quan Gong, Jun Du, Xiaoli Huang, Xiaogang Du
Summary: In this study, the histopathological changes, plasma ion levels, and transcriptome of gill tissue in Siberian sturgeon subjected to different temperatures were analyzed. The results showed that heat stress increased the levels of reactive oxygen species (ROS) and damage in the gill tissue. Transcriptome analysis revealed various genes related to osmoregulation, DNA repair, and apoptosis. This study provides insight into the effects of heat stress on gill tissue damage and osmoregulation in cold-water fishes.
Article
Biotechnology & Applied Microbiology
Wenjie Jin, Zixuan Li, Fengxia Ran, Shen Huang, Kefan Huo, Jianjuan Li, Qingshuo Han, Guojie Wang, Zhenji Wang, Shenlong Jian, Kemao Li, Changzhong Li
Summary: The study investigated the mechanism of copper toxicity in Gymnocypris eckloni using RNA-seq data from different tissues. Results showed that copper exposure led to different gene expression patterns in gills and liver, indicating oxidative stress and potential ribosome damage. The downregulation of global protein synthesis in response to metal-induced oxidative stress was observed, suggesting a selective translation mechanism in fish tissues.
Article
Biochemistry & Molecular Biology
Qiankun Liu, Pengtao Li, Shuang Cheng, Zilin Zhao, Yuling Liu, Yangyang Wei, Quanwei Lu, Jiangping Han, Xiaoyan Cai, Zhongli Zhou, Muhammad Jawad Umer, Renhai Peng, Baohong Zhang, Fang Liu
Summary: This study developed a method for single-cell RNA-seq based on isolating protoplast from cotton root tips and investigated the impact of salinity stress on gene expression. The results revealed many differentially expressed genes related to stress response and cellular metabolism after salt treatment. Additionally, salinity-responsive genes involved in plant hormone signaling and MAPK signaling were identified, as well as transcription factors associated with salt stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Ashutosh Das, Frank Panitz, Vivi Raundahl Gregersen, Christian Bendixen, Lars-Erik Holm
Article
Plant Sciences
Stephen L. Byrne, Istvan Nagy, Matthias Pfeifer, Ian Armstead, Suresh Swain, Bruno Studer, Klaus Mayer, Jacqueline D. Campbell, Adrian Czaban, Stephan Hentrup, Frank Panitz, Christian Bendixen, Jakob Hedegaard, Mario Caccamo, Torben Asp
Article
Agriculture, Dairy & Animal Science
H. P. Bertelsen, V. R. Gregersen, N. Poulsen, R. O. Nielsen, A. Das, L. B. Madsen, A. J. Buitenhuis, L. -E. Holm, F. Panitz, L. B. Larsen, C. Bendixen
JOURNAL OF ANIMAL SCIENCE
(2016)
Article
Hematology
Agnieszka Jendroszek, Malene S. Sonnichsen, Andres C. Munoz, Kato Leyman, Anni Christensen, Steen Vang Petersen, Tobias Wang, Christian Bendixen, Frank Panitz, Peter A. Andreasen, Jan K. Jensen
THROMBOSIS AND HAEMOSTASIS
(2017)
Article
Multidisciplinary Sciences
Zexi Cai, Bent Petersen, Goutam Sahana, Lone B. Madsen, Knud Larsen, Bo Thomsen, Christian Bendixen, Mogens Sando Lund, Bernt Guldbrandtsen, Frank Panitz
SCIENTIFIC REPORTS
(2017)
Article
Genetics & Heredity
Aniek C. Bouwman, Hans D. Daetwyler, Amanda J. Chamberlain, Carla Hurtado Ponce, Mehdi Sargolzaei, Flavio S. Schenkel, Goutam Sahana, Armelle Govignon-Gion, Simon Boitard, Marlies Dolezal, Hubert Pausch, Rasmus F. Brondum, Phil J. Bowman, Bo Thomsen, Bernt Guldbrandtsen, Mogens S. Lund, Bertrand Servin, Dorian J. Garrick, James Reecy, Johanna Vilkki, Alessandro Bagnato, Min Wang, Jesse L. Hoff, Robert D. Schnabel, Jeremy F. Taylor, Anna A. E. Vinkhuyzen, Frank Panitz, Christian Bendixen, Lars-Erik Holm, Birgit Gredler, Chris Hoze, Mekki Boussaha, Marie-Pierre Sanchez, Dominique Rocha, Aurelien Capitan, Thierry Tribout, Anne Barbat, Pascal Croiseau, Cord Drogemuller, Vidhya Jagannathan, Christy Vander Jagt, John J. Crowley, Anna Bieber, Deirdre C. Purfield, Donagh P. Berry, Reiner Emmerling, Kay-Uwe Goetz, Mirjam Frischknecht, Ingolf Russ, Johann Soelkner, Curtis P. Van Tassell, Ruedi Fries, Paul Stothard, Roel F. Veerkamp, Didier Boichard, Mike E. Goddard, Ben J. Hayes
Article
Multidisciplinary Sciences
Lingzhao Fang, Peter Sorensen, Goutam Sahana, Frank Panitz, Guosheng Su, Shengli Zhang, Ying Yu, Bingjie Li, Li Ma, George Liu, Mogens Sando Lund, Bo Thomsen
SCIENTIFIC REPORTS
(2018)
Article
Agriculture, Dairy & Animal Science
G. Sahana, B. Guldbrandtsen, B. Thomsen, L-E. Holm, F. Panitz, R. F. Brondum, C. Bendixen, M. S. Lund
JOURNAL OF DAIRY SCIENCE
(2014)
Article
Genetics & Heredity
Naveen Kumar Kadri, Goutam Sahana, Carole Charlier, Terhi Iso-Touru, Bernt Guldbrandtsen, Latifa Karim, Ulrik Sander Nielsen, Frank Panitz, Gert Pedersen Aamand, Nina Schulman, Michel Georges, Johanna Vilkki, Mogens Sando Lund, Tom Druet
Article
Evolutionary Biology
Andres Chana-Munoz, Agnieszka Jendroszek, Malene Sonnichsen, Tobias Wang, Michael Ploug, Jan K. Jensen, Peter A. Andreasen, Christian Bendixen, Frank Panitz
BMC EVOLUTIONARY BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Agnieszka Jendroszek, Jeppe B. Madsen, Andres Chana-Munoz, Daniel M. Dupont, Anni Christensen, Frank Panitz, Ernst-Martin Fuchtbauer, Simon C. Lovell, Jan K. Jensen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Correction
Multidisciplinary Sciences
Einar E. Nielsen, Alessia Cariani, Eoin Mac Aoidh, Gregory E. Maes, Ilaria Milano, Rob Ogden, Martin Taylor, Jakob Hemmer-Hansen, Massimiliano Babbucci, Luca Bargelloni, Dorte Bekkevold, Eveline Diopere, Leonie Grenfell, Sarah Helyar, Morten T. Limborg, Jann T. Martinsohn, Ross McEwing, Frank Panitz, Tomaso Patarnello, Fausto Tinti, Jeroen K. J. Van Houdt, Filip A. M. Volckaert, Robin S. Waples, Jan E. J. Albin, Juan M. Vieites Baptista, Vladimir Barmintsev, Jose M. Bautista, Christian Bendixen, Jean-Pascal Berge, Dietmar Blohm, Barbara Cardazzo, Amalia Diez, Montserrat Espineira, Audrey J. Geffen, Elena Gonzalez, Nerea Gonzalez-Lavin, Ilaria Guarniero, Marc Jerome, Marc Kochzius, Grigorius Krey, Olivier Mouchel, Enrico Negrisolo, Corrado Piccinetti, Antonio Puyet, Sergey Rastorguev, Jane P. Smith, Massimo Trentini, Veronique Verrez-Bagnis, Alexander Volkov, Antonella Zanzi, Mark de Bruyn, Gary R. Carvalho
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Alessio Soggiu, Cristian Piras, Stefano Gaiarsa, Emoke Bendixen, Frank Panitz, Christian Bendixen, Davide Sassera, Milena Brasca, Luigi Bonizzi, Paola Roncada
GENOME ANNOUNCEMENTS
(2015)
Article
Genetics & Heredity
Jeanette H. Jensen, Lone B. Madsen, Frank Panitz, Henrik Hornshoj, Rasmus O. Nielsen, Christian Bendixen, Niels Oksbjerg, Bo Thomsen