Article
Biochemistry & Molecular Biology
Gabriela Regina Rosa Souza, Milene Dalmina, Jelver Alexander Sierra Restrepo, Leonidas Joao de Mello Junior, Adny Henrique Silva, Ana Gualberto, Jacy Gameiro, Dalton Dittz, Andre Avelino Pasa, Frederico Pittella, Tania Beatriz Creczynski-Pasa
Summary: A hybrid nanocarrier composed of calcium phosphate and a block copolymer was successfully developed to deliver VEGF siRNA into metastatic breast cancer in mice, resulting in a significant decrease in tumor size. The study demonstrates the potential of this nanocarrier in achieving RNAi effect and tumor therapy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Review
Cell Biology
Mingyuan Zou, Yue Du, Ruizhen Liu, Zeliang Zheng, Jian Xu
Summary: Ovarian cancer is the fifth leading cause of cancer-related death in women in the United States, with limited success in early screening and poor overall prognosis. Recurrence remains a major challenge in treatment. Understanding the pathogenesis of ovarian cancer is essential for developing more effective treatments.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2021)
Article
Pharmacology & Pharmacy
Kiruthiga Chandramohan, Devasahayam Jaya Balan, Kasi Pandima Devi, Seyed Fazel Nabavi, Sara Reshadat, Maryam Khayatkashani, Sepideh Mahmoodifar, Rosanna Filosa, Niloufar Amirkhalili, Soroush Pishvaei, Omolbanin Sargazi-Aval, Seyed Mohammad Nabavi
Summary: Colorectal cancer is a common and deadly disease, especially among older individuals. Chemotherapy has negative effects on both normal and malignant cells. Various signaling pathways, such as Hh, JAK/STAT, Wnt/beta-catenin, TNF-beta, EGFR/MAPK, PI3K, NF-kappa B, and Notch, are involved in the development of colorectal cancer. This study focuses on innovative siRNA therapies and delivery methods for the safe and effective treatment of colorectal cancer.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Marco Hoffmann, Sven Gerlach, Masanari Takamiya, Samar Tarazi, Nils Hersch, Agnes Csiszar, Ronald Springer, Georg Dreissen, Hanno Scharr, Sepand Rastegar, Tanja Beil, Uwe Straehle, Rudolf Merkel, Bernd Hoffmann
Summary: Efficient and biocompatible transfer of nucleic acids into mammalian cells is a challenging task. Viral transduction is highly efficient but has safety concerns. Lipo- or polyplexes have low transfer efficiencies and may cause inflammatory responses. Using fusogenic liposomes, we achieved highly efficient and biocompatible transfer of RNA molecules, bypassing recognition mechanisms and minimizing inflammatory cytokine responses.
Article
Plant Sciences
Jinqiu Fu, Shijing Xu, Huan Lu, Fanchi Li, Shengchun Li, Ling Chang, David G. Heckel, Ralph Bock, Jiang Zhang
Summary: Plant-mediated RNA interference (RNAi) using dsRNA or siRNA has shown promise in pest control, but little is known about the molecular mechanisms. This study found that siRNAs derived from transgenic plants may be more effective triggers of RNAi in Lepidoptera than dsRNAs.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Pharmacology & Pharmacy
Ali Bishani, Darya M. Makarova, Elena V. Shmendel, Mikhail A. Maslov, Aleksandra V. Sen'kova, Innokenty A. Savin, Daniil V. Gladkikh, Marina A. Zenkova, Elena L. Chernolovskaya
Summary: This study investigated the impact of different delivery systems on the activities of immunostimulatory double-stranded RNA (isRNA). It was found that the structure of the delivery system plays a vital role in determining the response to isRNA, with complexes containing long-chain liposomes showing significant cytokine induction and antitumor activity. Non-pegylated liposomes also exhibited pronounced antiproliferative effects on B16 melanoma cells in vitro and exerted antitumor and hepatoprotective effects in vivo.
Article
Pharmacology & Pharmacy
Wesley L. Fotoran, Jamile Ramos da Silva, Christiane Glitz, Luis Carlos de Souza Ferreira, Gerhard Wunderlich
Summary: This study demonstrated that cationic lipid-encapsulated self-amplifying mRNA (samRNA) constructs could achieve efficient gene transfection and antibody production in mouse skin, using a Venezuelan equine encephalitis (VEE) vector. These results suggest that intradermal delivery of samRNA constructs with cationic lipid encapsulation is a feasible approach for developing malaria vaccines.
Article
Agricultural Engineering
Ran Xu, Litao Zhang, Wenjie Yu, Jianguo Liu
Summary: The study used transcriptomic data and a transformation system to downregulate the key enzyme alpha-1,6-mannosyltransferase (HpOCH1) in Haematococcus pluvialis cells, resulting in thinner cell walls and simplified astaxanthin extraction. The downregulation of HpOCH1 led to decreased cell wall organization and protection, but did not affect astaxanthin synthesis.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Nilam Malankar, Kirtikumar Kondhare, Kishan Saha, Mohit Mantri, Anjan Banerjee
Summary: PhasiRNA siRD29(-) regulates the expression of gibberellin biosynthesis gene StGA3ox3 during stolon-to-tuber transitions in potato.
Article
Immunology
Pierre Tonnerre, David Wolski, Sonu Subudhi, Jihad Al-Jabban, Ruben C. Hoogeveen, Marcos Damasio, Hannah K. Drescher, Lea M. Bartsch, Damien C. Tully, Debattama R. Sen, David J. Bean, Joelle Brown, Almudena Torres-Cornejo, Maxwell Robidoux, Daniel Kvistad, Nadia Alatrakchi, Ang Cui, David Lieb, James A. Cheney, Jenna Gustafson, Lia L. Lewis-Ximenez, Lucile Massenet-Regad, Thomas Eisenhaure, Jasneet Aneja, W. Nicholas Haining, Raymond T. Chung, Nir Hacohen, Todd M. Allen, Arthur Y. Kim, Georg M. Lauer
Summary: The study highlights the impact of T cell exhaustion on clearing chronic infections and malignant cells. Even after cure of HCV infection, exhausted T cells show durable exhaustion at functional, phenotypic, and transcriptional levels.
Article
Biochemistry & Molecular Biology
Andrew Varley, Jean-Paul Desaulniers
Summary: This article presents an improved scheme for synthesizing a mono-fluorinated phosphoramidite for nucleic acid modification. The purification, reaction, and biological activity of the modified strands are evaluated.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2023)
Article
Chemistry, Medicinal
Xiaoyu Huang, Ruonan Song, Xiao Wang, Kongfang He, Rumeng Shan, Fei Xie, Guihua Huang
Summary: This study aimed to develop poly-gamma-glutamic acid-modified cationic liposomes (gamma-PGA-CL) to deliver pemetrexed disodium (PMX) and siRNA for the treatment of non-small cell lung cancer (NSCLC). In vitro and in vivo experiments demonstrated that the combination had a significant inhibitory effect on tumor growth, proving its potential as a treatment strategy for NSCLC.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Review
Ophthalmology
Shibani Supe, Archana Upadhya, Kavita Singh
Summary: Ocular neovascularization plays a central role in various ocular diseases, and targeting VEGF with siRNA provides a promising new approach for treatment. The use of ocular anti-VEGF therapy has marked a new era in the treatment of retinal diseases. Delivery methods for siRNA protecting against degradation and suitable for long-term care are key to enhancing the effectiveness of RNAi-based anti-VEGF ocular therapies.
EXPERIMENTAL EYE RESEARCH
(2021)
Review
Engineering, Biomedical
Libo Zhou, Lee E. Rubin, Chuanju Liu, Yupeng Chen
Summary: Articular cartilage injury is a hallmark of arthritic diseases, difficult to repair and treat. Recent research suggests that RNA interference (RNAi) has the potential to repair damaged cartilage, but effective delivery of small RNAs remains a significant challenge. Nanomaterial delivery systems have been developed to enhance the efficacy of RNAi drugs for cartilage diseases.
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Andrea Arena, Eugenia Belcastro, Antonella Accardo, Annamaria Sandomenico, Olivia Pagliarosi, Elisabetta Rosa, Stefania Petrini, Libenzio Adrian Conti, Ezio Giorda, Tiziana Corsetti, Riccardo Schiaffini, Giancarlo Morelli, Alessandra Fierabracci
Summary: Autoimmune endocrine disorders such as type 1 diabetes and thyroiditis currently rely on hormone replacement therapy, highlighting the need for personalized immunotherapeutic strategies targeting T and B lymphocytes. This study improved upon a personalized immunotherapeutic approach by functionalizing liposomes with Rituximab antibody, specifically targeting B lymphocytes in conditions like type 1 diabetes. The results showed that these functionalized liposomes had a significant inhibitory effect on the function of B lymphocytes, suggesting potential applicability for personalized immunotherapy in other autoimmune disorders where B lymphocytes are the primary pathogenic immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Emmanuel de Billy, Marsha Pellegrino, Domenico Orlando, Giulia Pericoli, Roberta Ferretti, Pietro Businaro, Maria Antonietta Ajmone-Cat, Sabrina Rossi, Lucia Lisa Petrilli, Nicola Maestro, Francesca Diomedi-Camassei, Marco Pezzullo, Cristiano De Stefanis, Paola Bencivenga, Alessia Palma, Rossella Rota, Francesca Del Bufalo, Luca Massimi, Gerrit Weber, Chris Jones, Andrea Carai, Simona Caruso, Biagio De Angelis, Ignazio Caruana, Concetta Quintarelli, Angela Mastronuzzi, Franco Locatelli, Maria Vinci
Summary: This study investigated the anti-tumor activity of GD2-CAR T-cells in treating DMG/DIPG and identified the potential of using IGF1R/IR inhibitors in combination with GD2-CAR T-cells. The results showed that the dual IGF1R/IR antagonists BMS-754807 and linsitinib were able to inhibit tumor cell viability without affecting CAR T-cells. Linsitinib also improved the function of GD2-CAR T-cells. The combination of linsitinib and GD2-CAR T-cells demonstrated enhanced anti-tumor activity in vitro, ex vivo, and in vivo models of DIPG.
Article
Materials Science, Multidisciplinary
Elisabetta Petrucci, Francesco Porcelli, Monica Orsini, Serena De Santis, Giovanni Sotgiu
Summary: The development of mixed oxide electrodes is being intensively investigated to reduce cost and improve performance. In this study, thin-film electrodes coated with ruthenium and copper oxide were fabricated. The addition of copper enhanced the capacitive response and electron transfer reversibility, but the interlayer had a negative effect on charge storage power.
Review
Pharmacology & Pharmacy
Isabella Muscari, Alessandra Fierabracci, Sabrina Adorisio, Marina Moretti, Lorenza Cannarile, Emira Ayroldi, Vu Thi Minh Hong, Domenico V. Delfino
Summary: Glucocorticoids exert their pharmacological actions by mimicking and amplifying the function of the endogenous glucocorticoid system's canonical physiological stress response. They affect the immune system at the levels of inflammation and adaptive and innate immunity. Interactions between glucocorticoids and natural killer cells are critical for successful glucocorticoid therapy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sara Pagnotta, Antonella Tramutola, Eugenio Barone, Fabio Di Domenico, Valeria Pittala, Loredana Salerno, Valentina Folgiero, Matteo Caforio, Franco Locatelli, Stefania Petrini, D. Allan Butterfield, Marzia Perluigi
Summary: This study focuses on the role of BACH1/NRF2 ratio in the regulation of antioxidant response and its impact on patients with Down syndrome. The results show that overexpression of BACH1 disrupts the induction of antioxidant response genes, leading to oxidative damage accumulation. Additionally, administration of CAPE and VP961 promotes NRF2 nuclear translocation in cells from Down syndrome patients, improving antioxidant response.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Immunology
Andrea Arena, Eugenia Belcastro, Francesca Ceccacci, Stefania Petrini, Libenzio Adrian Conti, Olivia Pagliarosi, Ezio Giorda, Simona Sennato, Riccardo Schiaffini, Peng Wang, James C. Paulson, Giovanna Mancini, Alessandra Fierabracci
Summary: The C1858T variant of the PTPN22 gene is associated with autoimmune diseases. Liposomes carrying siRNA targeting the C1858T variant have been functionalized with a high affinity ligand of Siglec-10, resulting in enhanced delivery to immunocytes expressing Siglec-10. This approach effectively downregulates the variant PTPN22 gene and restores IL-2 secretion in T1D patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Alessandra Fierabracci, Eugenia Belcastro, Elena Carbone, Olivia Pagliarosi, Alessia Palma, Lucia Pacillo, Carmela Giancotta, Paola Zangari, Andrea Finocchi, Caterina Cancrini, Domenico Vittorio Delfino, Marco Cappa, Corrado Betterle
Summary: Autoimmune diseases are a diverse group of immune system disorders that can be associated with immunodeficiency. This study examined the presence of AIRE gene variations in patients with high polyautoimmunity complexity and found a significant association between the S278R polymorphism of the AIRE gene and APECED-like conditions. Additional genetic determinants need to be identified to improve diagnosis and targeted therapies for these patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Stefano Levi Mortera, Valeria Marzano, Pamela Vernocchi, Maria Cristina Matteoli, Valerio Guarrasi, Simone Gardini, Federica Del Chierico, Novella Rapini, Annalisa Deodati, Alessandra Fierabracci, Stefano Cianfarani, Lorenza Putignani
Summary: Recent research has shown a correlation between the composition of the human gut microbiota and the onset of type 1 diabetes. In this study, a comprehensive metaproteomic investigation was conducted on children with type 1 diabetes, revealing potential gut microbiota features related to the severity of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Giulia Fasano, Valentina Muto, Francesca Clementina Radio, Martina Venditti, Niloufar Mosaddeghzadeh, Simona Coppola, Graziamaria Paradisi, Erika Zara, Farhad Bazgir, Alban Ziegler, Giovanni Chillemi, Lucia Bertuccini, Antonella Tinari, Annalisa Vetro, Francesca Pantaleoni, Simone Pizzi, Libenzio Adrian Conti, Stefania Petrini, Alessandro Bruselles, Ingrid Guarnetti Prandi, Cecilia Mancini, Balasubramanian Chandramouli, Magalie Barth, Celine Bris, Donatella Milani, Angelo Selicorni, Marina Macchiaiolo, Michaela V. Gonfiantini, Andrea Bartuli, Riccardo Mariani, Cynthia J. Curry, Renzo Guerrini, Anne Slavotinek, Maria Iascone, Bruno Dallapiccola, Mohammad Reza Ahmadian, Antonella Lauri, Marco Tartaglia
Summary: Disruption to the ER-Golgi network can cause neurodevelopmental disorders, and ARF3 mutations have been identified as the cause of a rare pediatric neurological disorder. This study provides detailed molecular characterization of ARF3 mutations and demonstrates their impact on Golgi morphology, vesicles assembly, and trafficking.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Elisabetta Petrucci, Francesco Porcelli, Monica Orsini, Serena De Santis, Giovanni Sotgiu
Summary: This work focuses on the fabrication and characterization of electrodes coated with thin films of ruthenium and manganese oxide. The influence of different precursors on the morphology and electrochemical properties of the materials was investigated. The combination of RuCl3 and Mn(acac) shows outstanding capacitive properties.
Article
Chemistry, Multidisciplinary
Martina Marsotto, Serena De Santis, Giovanni Sotgiu, Chiara Battocchio, Giovanna Iucci, Anita Ceccucci, Giancarlo Masci, Monica Orsini
Summary: This study aims to prepare and characterize a versatile multifunctional platform for immobilizing macromolecules on a titanium surface using robust covalent grafting. Functionalized titanium is widely used in the biomedical field to improve its properties, but long-term stability is compromised due to inflammation and bacterial infections. The proposed method allows for the insertion of three different organic linkers that serve as anchors for biopolymers or bioactive molecules. The use of green solvents in this strategy offers an alternative to traditional solvent methods. Assessment through spectroscopic techniques confirms the successful modification of the titanium surface, and covalent attachment of two polymers shows potential for enhancing biocompatibility and extending the lifespan of biomedical implants.
Article
Materials Science, Biomaterials
Serena De Santis, Romualdo Varricchio, Anita Ceccucci, Giovanni Sotgiu, Alessandra Di Masi, Gabriele Magna, Simona Sennato, Monica Orsini
Summary: A cerium mixed oxide (CeOx) coating was successfully achieved on both pristine and nanotubular-structured Ti and Ti6Al4V surfaces using a simple drop-casting technique. The presence of CeOx on nanotube surfaces enhanced osseointegration, while on non-nanostructured surfaces, this coating improved resistance to oxidative stress and exhibited excellent antibacterial properties.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Marcello Niceta, Yuh-Charn Lin, Valentina Muto, Barbara Vona, Alistair T. Pagnamenta, Reza Maroofian, Christian Beetz, Hermine Van Duyvenvoorde, Maria Lisa Dentici, Peter Lauffer, Sadeq Vallian, Andrea Ciolfi, Simone Pizzi, Peter Bauer, Nana-Maria Gruning, Emanuele Bellacchio, Andrea Del Fattore, Stefania Petrini, Ranad Shaheen, Dov Tiosano, Rana Halloun, Ben Pode-Shakked, Hatice Mutlu Albayrak, Emregul Isik, Jan M. Wit, Marcus Dittrich, Bruna L. Freire, Debora R. Bertola, Alexander A. L. Jorge, Ortal Barel, Ataf H. Sabir, Amal M. Al Teneiji, Sulaima M. Taji, Nouriya Al-Sannaa, Hind Al-Abdulwahed, Maria Cristina Digilio, Melita Irving, Yair Anikster, Gandham S. L. Bhavani, Katta M. Girisha, Thomas Haaf, Jenny C. Taylor, Bruno Dallapiccola, Fowzan S. Alkuraya, Ruey-Bing Yang, Marco Tartaglia
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Pediatrics
Riccardo Schiaffini, Andrea Campana, Annalisa Deodati, Emanuela Peschiaroli, Maria Francesca Lanzillotta, Alessandra Fierabracci
Summary: This study reported a case of a child diagnosed with Type 1 diabetes during a paucisymptomatic COVID-19 infection, with well-controlled metabolic status throughout. The study speculated that SARS-CoV-2 infection may have acted as a precipitating factor in the disease, but the fundamental triggering event of the autoimmune disease may have occurred even before the infection.
ITALIAN JOURNAL OF PEDIATRICS
(2022)