Article
Food Science & Technology
Yuelin Chen, Meitong Liu, Hongdi Wei, Jiakang Guo, Shengzhuo Zhang, Xiujuan Bu, Shanshan Chen, Duoduo Zhang, Shuang Guan
Summary: Excessive alcohol consumption can lead to serious liver injury, particularly through a form of programmed cell death called necroptosis. This study aimed to uncover the mechanisms behind this process and found that ethanol activates RIPK1 and RIPK3, leading to necroptosis in hepatocytes. Additionally, autophagy was activated in ethanol-treated hepatocytes. The researchers also discovered that LC3, an autophagy marker protein, plays a crucial role in ethanol-induced necroptosis. Knocking down LC3 or using an autophagy inhibitor alleviated necrosome formation and necroptosis in hepatocytes. These findings provide new insights into the complex mechanisms involved in the development of acute alcoholic liver injury.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Christopher von Beek, Linnea Alriksson, Josefine Palle, Ann-Marie Gustafson, Mirjana Grujic, Fabio Rabelo Melo, Mikael E. Sellin, Gunnar Pejler
Summary: The study investigated the effects of dynamin inhibition on pediatric acute leukemia cells, showing that the inhibitors can reduce proliferation and induce apoptosis, but had no effect on human bone marrow biopsies or tumor formation in mice. These findings suggest potential benefits and limitations of using dynamin inhibition in leukemia treatment strategies.
Article
Cell Biology
Sandy Ibanes, Fatima El-Alaoui, Josephine Lai-Kee-Him, Chantal Cazevieille, Francois Hoh, Sebastien Lyonnais, Patrick Bron, Luca Cipelletti, Laura Picas, Simonetta Piatti
Summary: This study reveals that Syp1 is able to align and pack septin filaments laterally, aiding in the assembly of septin collar. It also emphasizes the conserved protein functions and the functional diversity of unstructured protein domains.
Article
Multidisciplinary Sciences
Naiqian Zhao, Huiwen Tan, Li Wang, Le Han, Yanli Cheng, Ying Feng, Ting Li, Xiaoling Liu
Summary: The study demonstrates that FoxO1 plays a crucial role in protecting hepatocytes from palmitate-induced fat accumulation by regulating ATGL-dependent lipolysis. Palmitate promotes lipid accumulation but inhibits lipid decomposition, which can be reversed by FoxO1 overexpression.
Article
Endocrinology & Metabolism
Han Zeng, Hong Qin, Meng Liao, Enze Zheng, Xiaoqing Luo, Anhua Xiao, Yiyu Li, Lin Chen, Li Wei, Lei Zhao, Xiong Z. Ruan, Ping Yang, Yaxi Chen
Summary: This study found that CD36 plays a role in the development of NAFLD by facilitating the uptake of free fatty acids and enhancing de novo lipogenesis in hepatocytes. CD36 forms a complex with INSIG2, disrupting the interaction between SCAP and INSIG2, leading to the translocation of SREBP1 for processing. These findings provide new evidence for targeting CD36 as a potential therapeutic approach for NAFLD.
MOLECULAR METABOLISM
(2022)
Article
Toxicology
Yue Xu, Shangwen Wang, Chi-Kwan Leung, Hao Chen, Chan Wang, Huijie Zhang, Shuwei Zhang, Yi Tan, Haowei Wang, Lin Miao, Yi Li, Yizhen Huang, Xiaoxing Zhang, Genmeng Yang, Ruilin Zhang, Xiaofeng Zeng
Summary: Amanitin poisoning is a dangerous mushroom poisoning characterized by the toxic effects of alpha-Amanitin on the liver. The mechanism by which alpha-Amanitin induces liver injury and the role of autophagy in this process are not fully understood.
TOXICOLOGY LETTERS
(2023)
Article
Cell Biology
Caihua Dong, Xinying Li, Jiao Yang, Detian Yuan, Yuanshuai Zhou, Yina Zhang, Guohua Shi, Ruobing Zhang, Jianping Liu, Peng Fu, Minxuan Sun
Summary: High expression of PPFIBP1 is associated with enhanced invasion and poor prognosis in GBM patients, promoting cell invasion and migration by regulating differential gene clusters involved in Wnt and adhesion-related signaling pathways. PPFIBP1 activates FAK, Src, JNK, and c-Jun to enhance MMP-2 expression, and inhibiting phosphorylation of Src and FAK reverses PPFIBP1-induced invasion and migration in GBM cells.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Chun-Te Ho, Shih-Pei Lin, Ling-Ming Tseng, Mien-Chie Hung, Shih-Chieh Hung
Summary: This study found that a mutant form of Snail reduced the expression of Src in luminal A breast cancer cells and enhanced their migration and invasion abilities, while reducing their capacity to form tumor spheres. Additionally, human luminal A breast cancer samples showed a negative correlation between Vimentin and Src expression.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Environmental Sciences
Weiqi Sun, Zhijing Ni, Rui Li, Xiuli Chang, Weihua Li, Mingjun Yang, Zhijun Zhou
Summary: The study showed that flurochloridone induces apoptosis in TM4 cells through the mitochondrial apoptotic pathway, accompanied by accumulation of ROS, depolarization of the mitochondrial membrane potential, etc. Pretreatment with the ROS scavenger NAC significantly alleviated flurochloridone-induced cell apoptosis and MMP depolarization.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Gastroenterology & Hepatology
Li Chen, Jun Dong, Siyang Liao, Siyou Wang, Zhida Wu, Meiling Zuo, Bing Liu, Chaojun Yan, Yong Chen, He He, Qingtao Meng, Zhiyin Song
Summary: Our study reveals the critical role of Sam50 in maintaining mitochondrial membrane integrity and mtDNA stability in hepatocytes. Sam50 depletion induces cardiolipin externalization, which triggers mtDNA release and activates mtDNA-dependent innate immunity.
Article
Immunology
Christian Binder, Felix Sellberg, Filip Cvetkovski, Stefan Berg, Erik Berglund, David Berglund
Summary: The study showed that siplizumab increased NK cell activation by binding to Fc gamma RIIIA, suggesting potential application in inhibiting NK cell activation. However, the inhibitory effects of CD2 blockade on natural cytotoxicity and antibody-dependent cytotoxicity remain unclear.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Feixia Wang, Li Tang, Baoyu Liang, Chun Jin, Liyuan Gao, Yujia Li, Zhanghao Li, Jiangjuan Shao, Zili Zhang, Shanzhong Tan, Feng Zhang, Shizhong Zheng
Summary: Our study demonstrated that Yi-Qi-Jian-Pi Formula effectively ameliorates liver injury in vivo and in vitro by regulating hepatocyte necroptosis, through targeting RIPK1/RIPK3-complex-dependent necroptosis via ROS signaling. This suggests a positive role for YQJPF in the treatment of ACLF.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Zhihui Li, Linhao Li, Scott Heyward, Shuaiqian Men, Meishu Xu, Tatsuya Sueyoshi, Hongbing Wang
Summary: The study reveals that phenobarbital significantly elevates the expression of SLC13A5 in human primary hepatocytes through the activation of PXR but not CAR.
Article
Gastroenterology & Hepatology
Akitoshi Sano, Eiji Kakazu, Shin Hamada, Jun Inoue, Masashi Ninomiya, Tomoaki Iwata, Mio Tsuruoka, Kosuke Sato, Atsushi Masamune
Summary: This study found that an imbalance of free amino acids can exacerbate hepatic steatosis by interfering with the oxidative stress sensor system, involving Nrf2 and VLDL-related lipid metabolism. Results suggest that modulation of the Keap1-Nrf2 system can improve liver damage caused by this imbalance.
Article
Immunology
Junjun Guo, Meijuan Zhou, Man Zhao, Shuxian Li, Zhenya Fang, Anna Li, Meihua Zhang
Summary: Through the study, it was found that TIGAR is up-regulated under high glucose conditions in vivo and in vitro, and loss of TIGAR increases ROS levels in trophoblast cells, leading to phenotypic changes and inhibition of migration, invasion, and tube formation. This switch is caused by the activation of the NLRP3-ASC-caspase-1 signaling pathway, resulting in pyroptosis. These findings can be reversed by antioxidant treatment and receptor blockage. In conclusion, trophoblast pyroptosis is an upstream event of TIGAR deficiency-induced inflammation, which is promoted by ROS accumulation through NLRP3-ASC inflammasome.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Veronica Riccioni, Flavia Trionfetti, Claudia Montaldo, Sabrina Garbo, Francesco Marocco, Cecilia Battistelli, Alessandra Marchetti, Raffaele Strippoli, Laura Amicone, Carla Cicchini, Marco Tripodi
Summary: This study reveals the role of hnRNP-Q in epithelial-mesenchymal transition (EMT) and its regulation of a set of miRNAs, which in turn influences the cellular phenotype and migratory capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Eleonora Cimini, Germana Grassi, Alessia Beccacece, Rita Casetti, Concetta Castilletti, Maria Rosaria Capobianchi, Emanuele Nicastri, Chiara Agrati
Summary: Gamma delta T cells play a role in eliminating pathogens and infected/tumoral cells. Their role in Dengue Viral Infection (DENV) is not fully understood. This study characterized V delta 2 T cells in DENV patients and found that DENV infection reduced their frequency and increased activation and exhaustion markers. The functionality of V delta 2 T cells, especially IFN-gamma production, was also affected. Further studies are needed to understand the clinical impact of gamma delta T cell impairment in disease severity and their antiviral and immunoregulatory activities.
Article
Computer Science, Interdisciplinary Applications
Rita Murri, Carlotta Masciocchi, Jacopo Lenkowicz, Massimo Fantoni, Andrea Damiani, Antonio Marchetti, Paolo Domenico Angelo Sergi, Giovanni Arcuri, Alfredo Cesario, Stefano Patarnello, Massimo Antonelli, Rocco Bellantone, Roberto Bernabei, Stefania Boccia, Paolo Calabresi, Andrea Cambieri, Roberto Cauda, Cesare Colosimo, Filippo Crea, Ruggero De Maria, Valerio De Stefano, Francesco Franceschi, Antonio Gasbarrini, Raffaele Landolfi, Ornella Parolini, Luca Richeldi, Maurizio Sanguinetti, Andrea Urbani, Maurizio Zega, Giovanni Scambia, Vincenzo Valentini
Summary: This study describes an AI-based COVID-19 data management system that utilizes real-time, centralized, and standardized patient data to improve COVID-19 patient management and clinical outcomes. The system includes a large collection of clinical data and a real-time updated dashboard to support COVID-19 patient management at different levels and promote disease prediction research. This approach has great potential for application in similar situations.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Michela Terri, Nicoletta Mancianti, Flavia Trionfetti, Bruno Casciaro, Valeria de Turris, Giammarco Raponi, Giulio Bontempi, Claudia Montaldo, Alessandro Domenici, Paolo Mene, Maria Luisa Mangoni, Raffaele Strippoli
Summary: The study aimed to analyze the effects of blue LED on both bacteria and human keratinocytes. The results demonstrated that blue LED had cytotoxic activity against bacteria and induced complex responses in keratinocytes, including cell cycle arrest, chromatin damage, and epithelial to mesenchymal transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Laura Amicone, Alessandra Marchetti, Carla Cicchini
Summary: This review focuses on the role of exosome-associated circRNAs as key regulators of EMT in cancer. The relevance of these molecules in regulating the intercellular communication in TME and tumor progression is highlighted. Moreover, it suggests that exosome-associated circRNA modulation should be taken into account for cancer diagnostic and therapeutic approaches.
Review
Biochemistry & Molecular Biology
Sabrina Garbo, Rossella Maione, Marco Tripodi, Cecilia Battistelli
Summary: The increasing understanding of non-coding RNAs (ncRNAs) and their functional roles has sparked great interest in the scientific community. This review summarizes the therapeutic potential of ncRNA in disease treatment and discusses the advantages and limitations of RNA therapeutics, as well as the development of targeted delivery strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Elisabetta Di Bello, Veronica Sian, Giulio Bontempi, Clemens Zwergel, Rossella Fioravanti, Beatrice Noce, Carola Castiello, Stefano Tomassi, Davide Corinti, Daniela Passeri, Roberto Pellicciari, Ciro Mercurio, Mario Varasi, Lucia Altucci, Marco Tripodi, Raffaele Strippoli, Angela Nebbioso, Sergio Valente, Antonello Mai
Summary: After years of research, HDAC inhibitors have been developed and approved by FDA/Chinese FDA for the treatment of cancer and non-cancer diseases. A series of novel compounds have been discovered to be effective anticancer agents, demonstrating selective inhibition of HDACs and inducing cell death and differentiation. These compounds also modulate gene and protein expression related to apoptosis and show potent antiproliferative activity in various cancer cell lines.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Francesca Ippolito, Veronica Consalvi, Valeria Noce, Cecilia Battistelli, Carla Cicchini, Marco Tripodi, Laura Amicone, Alessandra Marchetti
Summary: In this study, the researchers discovered that the extracellular signal-regulated kinase 5 (ERK5) plays a role in regulating the activity of YES-associated protein (YAP). They demonstrated this through inhibition/silencing and overexpression experiments using liver stem cells, hepatocytes, and hepatocellular carcinoma (HCC) cell lines. The researchers also identified the mechanism by which ERK5 controls YAP activity and its involvement in various cellular processes. Overall, this study provides new insights into the regulation of YAP and suggests ERK5 as a potential therapeutic target.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Giulio Bontempi, Michela Terri, Sabrina Garbo, Claudia Montaldo, Davide Mariotti, Veronica Bordoni, Sergio Valente, Clemens Zwergel, Antonello Mai, Alessandra Marchetti, Alessandro Domenici, Paolo Mene, Cecilia Battistelli, Marco Tripodi, Raffaele Strippoli
Summary: Histone deacetylase 1 (HDAC1) inhibition plays an important role in reversing fibrosis. The study found that HDAC1 inhibition can reverse cell-to-cell transformation and limit cell migration and invasion by promoting the expression of miR-769-5p. Additionally, miR-769-5p targets and regulates the expression of several crucial genes.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Flavia Trionfetti, Vanessa Marchant, Guadalupe. T. T. Gonzalez-Mateo, Edyta Kawka, Laura Marquez-Exposito, Alberto Ortiz, Manuel Lopez-Cabrera, Marta Ruiz-Ortega, Raffaele Strippoli
Summary: The global incidence of chronic kidney disease (CKD) is increasing, leading to a significant number of CKD patients developing end-stage renal disease (ESRD) and requiring kidney replacement therapies (KRT). Peritoneal dialysis (PD) is a convenient home therapy for KRT, but it can lead to damage of the peritoneal membrane (PM) due to the exposure to PD fluids containing high concentrations of glucose or other osmotic agents. This damage involves inflammation and fibrosis, which can be worsened by peritonitis episodes. In this article, we review the role of immune cells in PM damage during KRT and infections, as well as the anti-inflammatory properties of current clinical treatments for CKD patients in KRT and their potential effect on preserving PM integrity. Additionally, we discuss the implications of COVID-19 in CKD and KRT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Tania B. B. Lopez-Mendez, Miguel Sanchez-Alvarez, Flavia Trionfetti, Jose L. Pedraz, Marco Tripodi, Marco Cordani, Raffaele Strippoli, Juan Gonzalez-Valdivieso
Summary: In recent years, nanotechnology has provided new tools for the more effective treatment of cancer. Specifically, advances in biomaterials designed for drug delivery have the potential to overcome the limitations and side effects of traditional therapies. However, the use of autophagy in cancer treatment is currently limited due to contextual effects, low bioavailability, and non-targeted delivery of autophagy modulatory compounds. By combining the characteristics of nanoparticles with autophagy modulators, these drugs may become safer and more effective for cancer treatment.
CELL AND BIOSCIENCE
(2023)
Article
Chemistry, Medicinal
Clemens Zwergel, Michele Aventaggiato, Sabrina Garbo, Elisabetta Di Bello, Bruno Fassari, Beatrice Noce, Carola Castiello, Chiara Lambona, Federica Barreca, Dante Rotili, Rossella Fioravanti, Thomas Schmalz, Michael Weyand, Amelie Niedermeier, Marco Tripodi, Gianni Colotti, Clemens Steegborn, Cecilia Battistelli, Marco Tafani, Sergio Valente, Antonello Mai
Summary: The mitochondrial protein SIRT3 plays a role in cancer, metabolism, and hypoxia-related diseases. New 1,4-dihydropyridine compounds, namely 2 and 3, have been discovered and compound 3 is a specific activator of SIRT3. Among a series of related compounds, compound 3c binds and activates SIRT3 the strongest, while compound 3d shows the best effects on enhancing glutamate dehydrogenase activity and deacetylating proteins in breast cancer cells. Compound 3d also exhibits significant anti-cancer effects in both normoxia and hypoxia conditions, reducing cell viability, clonogenicity, and migration ability.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)