Article
Endocrinology & Metabolism
Evelyn Lara, Nathaly Rivera, Alejandro Gonzalez-Bernal, Daniela Rojas, Daniela Lopez-Espindola, Andres Rodriguez, Carlos Escudero
Summary: This study evaluated brain microvascular perfusion and microvascular reactivity in offspring born to mice with preeclampsia-like syndrome. It found that the offspring exhibited impaired brain perfusion and microvascular reactivity, particularly in female mice, associated with differential expression of angiogenic proteins in the brain tissue.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Endocrinology & Metabolism
Claire Richards, Kimberly Sesperez, Michael Chhor, Sahar Ghorbanpour, Claire Rennie, Clara Liu Chung Ming, Chris Evenhuis, Valentina Nikolic, Natasa Karadzov Orlic, Zeljko Mikovic, Milan Stefanovic, Zoran Cakic, Kristine McGrath, Carmine Gentile, Kristen Bubb, Lana McClements
Summary: The rat RUPP model induced cardiac, renal, and placental features reflective of preeclampsia. FKBPL was increased in the hearts of RUPP rats and cardiac spheroids treated with plasma from women with preeclampsia, potentially indicating restricted angiogenesis and inflammation in this disorder. Understanding these novel FKBPL mechanisms in cardiac health in preeclampsia could be crucial in preventing future CVD.
BIOLOGY OF SEX DIFFERENCES
(2021)
Article
Physiology
Jesse N. Cottrell, Alexis C. Witcher, Kyleigh Comley, Mark W. Cunningham, Tarek Ibrahim, Denise C. Cornelius, Babbette LaMarca, Lorena M. Amaral
Summary: The study demonstrates that PIBF can improve hypertension caused by preeclampsia by reducing inflammation and enhancing fetal growth, which has been confirmed in a rat model.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jaimy Saif, Shakil Ahmad, Homira Rezai, Karina Litvinova, Anna Sparatore, Faisal A. Alzahrani, Keqing Wang, Asif Ahmed
Summary: An imbalanced angiogenic growth factors and poor utero-placental perfusion are strongly correlated with preeclampsia. A refined RUPP model in C57B1/6 J mice was developed to test the efficacy of MZe786 as a potential inhibitor of soluble Flt-1 for preeclampsia therapy. The study demonstrated that MZe786 treatment successfully prevented the development of preeclampsia by reducing maternal MAP and kidney nitrotyrosine staining, and improving fetal outcomes.
Article
Radiology, Nuclear Medicine & Medical Imaging
Christina. L. L. Herrera, Yiming Wang, Durga Udayakumar, Yin Xi, Quyen. N. N. Do, Matthew. A. A. Lewis, David. M. M. Owen, Baowei Fei, Catherine. Y. Y. Spong, Diane. M. M. Twickler, Ananth. J. J. Madhuranthakam
Summary: This study evaluates placental perfusion in normal pregnancies and pregnancies affected by chronic hypertension using pseudo-continuous arterial spin-labeled (pCASL) MRI. The results show a decline in placental perfusion in the second trimester of normal pregnancies, while it remained unchanged in cHTN pregnancies. Additionally, significantly lower placental perfusion was observed in pregnancies with infants that were small for gestational age. Therefore, pCASL-MRI-measured perfusion may serve as an effective imaging biomarker for placental insufficiency.
EUROPEAN RADIOLOGY
(2023)
Article
Cell Biology
Evangeline Deer, Lorena M. Amaral, Nathan Campbell, Sarah Fitzgerald, Owen Herrock, Tarek Ibrahim, Babbette LaMarca
Summary: IL-2, released from CD4+T cells, has dual actions in potentiating or quelling inflammatory response based on its concentration. In a PE rat model, low-dose recombinant IL-2 was shown to decrease blood pressure and normalize multi-organ mitochondrial dysfunction caused by placental ischemia.
Article
Obstetrics & Gynecology
Lina Bergman, Catherine Cluver, Niclas Carlberg, Michael Belfort, Mary C. Tolcher, Ronney B. Panerai, Teelkien van Veen
Summary: Dynamic cerebral autoregulation and cerebral perfusion pressure are altered in pregnancies complicated by preeclampsia, which may be important in the pathophysiological pathway and constitute a therapeutic target in the prevention of cerebral complications in preeclampsia.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2021)
Article
Nutrition & Dietetics
Faisal Altemani, Helen L. Barrett, Leonie K. Callaway, H. David McIntyre, Marloes Dekker Nitert
Summary: The abundance of nitrate-reducing bacteria in the oral microbiota is reduced in women who develop preeclampsia, indicating a potential pathway for prevention.
Article
Medicine, Research & Experimental
Xiaoguo Zheng, Yahan Lian, Jing Zhou, Qian Zhou, Yu Zhu, Chunhua Tang, Ping Zhang, Xinzhi Zhao
Summary: Preeclampsia (PE) has significant effects on the functional organization of the placental methylome, especially in terms of partially methylated domains (PMDs) and low-methylated regions (LMRs). The RUPP rat model of PE was found to accurately reflect the epigenetic mechanisms underlying human PE, with notable changes in PMDs, indicative of perturbations in the placental methylome. The changes in DNA methylation primarily occurred in LMRs, highlighting the disruption of enhancer DNA methylation due to placental ischemia. This emphasizes the importance of studying enhancer regions in future PE research.
Review
Biochemistry & Molecular Biology
Katarzyna Pankiewicz, Anna Fijalkowska, Tadeusz Issat, Tomasz M. Maciejewski
Summary: Preeclampsia is a complex and multifaceted syndrome with at least several potential pathways leading to its development. The two-stage model suggests that impaired placentation in early pregnancy leads to reduced placental perfusion, while the second stage involves the onset of maternal clinical symptoms, possibly through novel pathways. Recent advances focus on uterine artery remodeling alterations and the role of microRNAs in preeclampsia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Abdoulaye Issotina Zibrila, Yubei Li, Zheng Wang, Gongxiao Zhao, Haotian Liu, Jing Leng, Md Ahasan Ali, James Ampofo Osei, Yu-Ming Kang, Jinjun Liu
Summary: The study found that PYR can improve symptoms in rat PE models by attenuating placental ischemia and TNF-alpha-induced inflammation and prohypertensive activity in the PVN. This broadens the therapeutic potential of PYR in PE.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Perez-Roque, Elena Nunez-Gomez, Alicia Rodriguez-Barbero, Carmelo Bernabeu, Jose M. Lopez-Novoa, Miguel Pericacho
Summary: Preeclampsia is a pregnancy-specific disease characterized by hypertension, with its etiopathogenesis related to abnormal placentation and the release of soluble factors. High plasma levels of soluble endoglin (sEng) may serve as an early diagnostic and prognostic biomarker for the disease, impacting the pathophysiology of preeclampsia significantly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Ye Young Shin, Sung-Min An, Jea Sic Jeong, Seung Yun Yang, Geun-Shik Lee, Eui-Ju Hong, Eui-Bae Jeung, Seung Chul Kim, Beum-Soo An
Summary: The induction of pre-eclampsia conditions dysregulates steroid hormones through the regulation of steroidogenic enzymes, depending on specific pre-eclampsia symptoms.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Medicine, General & Internal
Deborah Jaeger, Jonathan Koger, Helene Duhem, Caroline Fritz, Victor Jeangeorges, Kevin Duarte, Bruno Levy, Guillaume Debaty, Tahar Chouihed
Summary: The study compared the effects of reduced doses of adrenaline with the recommended 1 mg dose on CPP in a pig model of cardiac arrest. The results showed that the 0.25 mg dose did not achieve the threshold of 35 mmHg, while the 0.5 mg dose and the standard 1 mg dose did not have significant differences in CPP.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Norman Shreeve, Delphine Depierreux, Delia Hawkes, James A. Traherne, Ulla Sovio, Oisin Huhn, Jyothi Jayaraman, Amir Horowitz, Hormas Ghadially, John R. B. Perry, Ashley Moffett, John G. Sled, Andrew M. Sharkey, Francesco Colucci
Summary: Genetic ablation of NKG2A in dams mated with wild-type males may result in suboptimal maternal vascular responses during pregnancy, leading to various abnormalities similar to human preeclampsia. The study also suggests that the maternal HLA-B allele plays a significant role in influencing NKG2A education, potentially affecting the risk of developing preeclampsia.
Article
Engineering, Chemical
Satomi Hirose, Jean-Paul Rieu, Olivier Cochet-Escartin, Christophe Anjard, Kenichi Funamoto
Summary: Spatiotemporal variations of oxygen concentration impact cell behaviors involved in physiological and pathological events. For Dd cells, oxygen-dependent characteristics affect their migration properties, such as migration frequency, velocity, and direction, depending on oxygen levels and gradients.
Article
Engineering, Biomedical
Namareq Widatalla, Kiyoe Funamoto, Motoyoshi Kawataki, Chihiro Yoshida, Kenichi Funamoto, Masatoshi Saito, Yoshiyuki Kasahara, Ahsan Khandoker, Yoshitaka Kimura
Summary: This study used a mathematical model to estimate the QT intervals in fetal mice and validated the results with Doppler ultrasound measurements, showing good agreement between the two.
BIOMEDICAL ENGINEERING ONLINE
(2022)
Article
Medicine, Research & Experimental
Sadayoshi Ito, Yasuyuki Okuda, Kotaro Sugimoto
Summary: This study examined factors associated with the antihypertensive effects of esaxerenone and the incidence of serum potassium elevation in patients with hypertension. Results showed that factors such as sex, weight, hypertension duration, prior antihypertensive treatment, plasma aldosterone concentration, urinary albumin-to-creatinine ratio, and smoking were associated with changes in blood pressure. Factors significantly associated with serum potassium elevation included baseline serum potassium, renal function, urinary albumin-to-creatinine ratio, blood pressure, and hypertension grade. The study concluded that esaxerenone is effective for patients with various backgrounds, but careful management is needed for those at risk of elevated serum potassium.
ADVANCES IN THERAPY
(2023)
Article
Multidisciplinary Sciences
Daisuke Saito, Ryosuke Tadokoro, Arata Nagasaka, Daisuke Yoshino, Takayuki Teramoto, Kanta Mizumoto, Kenichi Funamoto, Hinako Kidokoro, Takaki Miyata, Koji Tamura, Yoshiko Takahashi
Summary: Unlike mammals, avian early embryos have PGCs that utilize blood circulation to migrate to the somatic gonadal primordium. Our research shows that PGCs are arrested at a specific site in the capillary plexus, enabled by increased stiffness mediated by actin polymerization. They then soften to squeeze through the endothelial lining.
Article
Acoustics
Anam Bhatti, Takuro Ishii, Naoya Kanno, Hayato Ikeda, Kenichi Funamoto, Yoshifumi Saijo
Summary: Observing alterations in cutaneous vasculature can be a potential diagnostic marker for diseases. High-frequency ultrasound (HFUS) has been used in dermatology to observe skin morphologies and tissue conditions, but its ability to visualize micro-vessels is limited. In this study, a novel region-based SVD filtering approach was proposed to visualize cutaneous vascular networks. Experimental observations showed that this approach improved the image quality and has potential applications in the diagnosis of cutaneous disorders.
Article
Acoustics
Muhammad Shiddiq Sayyid Hashuro, Simon Tupin, Narendra Kurnia Putra, Kotaro Daibo, Kosuke Inoue, Takuro Ishii, Hiroyuki Kosukegawa, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta
Summary: A transparent ultrasound flow phantom material composed of poly(vinyl alcohol) hydrogel (PVA-H) with dimethyl sulfoxide (DMSO) and water solution was proposed for visualizing and measuring flow inside ultrasound systems. The material's refractive index was modified to match that of glass for transparency. Feasibility tests were conducted, and an ultrasound flow phantom was fabricated for B-mode visualization and Doppler-PIV comparison. The results showed promising accuracy in velocity measurement and similarity to real tissue visualization, with some limitations regarding sound velocity and Doppler measurements.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Naoyuki Takahashi, Daisuke Yoshino, Ryuji Sugahara, Satomi Hirose, Kazuki Sone, Jean-Paul Rieu, Kenichi Funamoto
Summary: This study developed a microfluidic platform for reconstructing hypoxic vascular microenvironments, by simultaneously applying hypoxic stress and fluid shear stress. The results showed that under the simultaneous exposure to hypoxic stress and fluid shear stress, the migration velocity of endothelial cells increased and gradually decreased, reaching the lowest value. Meanwhile, the cells aligned and elongated in the flow direction, with enhanced expression of VE-cadherin and actin filament assembly. Therefore, this microfluidic platform is useful for investigating the dynamics of endothelial cells in vascular microenvironments.
SCIENTIFIC REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Suguru Miyauchi, Koichi Hosoi, Shingo Tsuda, Toshiyuki Hayase, Kenichi Funamoto
Summary: This study investigated the effects of complex internal structures and torsional motion on left ventricular hemodynamics using computational fluid dynamics analyses. The presence of trabeculae carneae and papillary muscles decreased average wall shear stress and increased the relative residence time of blood stagnation at the apex of the left ventricle. Torsional motion caused irregular changes to the relative residence time level and distribution at the apex. Therefore, the complex internal structures and torsional motion of the left ventricle could cause blood stagnation.
Article
Cell Biology
Satomi Hirose, Julie Hesnard, Nasser Ghazi, Damien Roussel, Yann Voituron, Oliver Cochet-Escartin, Jean-Paul Rieu, Christophe Anjard, Kenichi Funamoto
Summary: Spatial and temporal variations of oxygen environments affect cell behaviors, and the aerotaxis of Dictyostelium discoideum seems to be an effective strategy for survival. Flavohemoglobins, potential oxygen sensors and oxidative stress modulators, play a role in this phenomenon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Ayumu Konno, Hirokazu Hirai, Taku Saito, Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa, Takamasa Sakai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
Summary: Vertically oscillating head motions can reduce blood pressure in hypertensive individuals, possibly by reducing the expression of angiotensin II type-1 receptor in astrocytes. This suggests that mechanical interventions could be used to elicit antihypertensive effects.
NATURE BIOMEDICAL ENGINEERING
(2023)
Meeting Abstract
Peripheral Vascular Disease
Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
JOURNAL OF HYPERTENSION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Yasuhiro Sawada, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Ayumu Konno, Hirokazu Hirai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao
Review
Engineering, Biomedical
Makoto Ohta, Naoya Sakamoto, Kenichi Funamoto, Zi Wang, Yukiko Kojima, Hitomi Anzai
Summary: Vascular endothelial cells are exposed to mechanical stress from blood flow and their functions can be studied using specific chamber designs. The functions of these cells are closely connected to the outer layers of arteries and organs, and the interaction between smooth muscle cells in the middle layer and endothelial cells can be investigated using coculture chambers. Additionally, microfabrication technology in microfluidic devices allows for mechanical and chemical stimulation of cells to study their dynamics in vivo microenvironments.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Proceedings Paper
Engineering, Biomedical
S. Hirose, J-P Rieu, C. Anjard, O. Cochet-Escartin, H. Kikuchi, K. Funamoto
Summary: The study reveals that Dd exhibits aerotaxis, migrating towards higher oxygen concentrations, and shows aerokinesis by adjusting migration velocity based on oxygen concentration, with significant effects observed in low oxygen environments.
2021 43RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY (EMBC)
(2021)
Article
Cell Biology
Satomi Hirose, Yugo Tabata, Kazuki Sone, Naoyuki Takahashi, Daisuke Yoshino, Kenichi Funamoto
Summary: Oxygen tension affects the collective migration of vascular endothelial cells by regulating VE-cadherin through the PAK pathway, as well as other mechanisms via HIF-1 alpha, especially under extreme hypoxic conditions.
CELL ADHESION & MIGRATION
(2021)