Article
Immunology
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Summary: This study provides evidence that HutC protein in Vibrio parahaemolyticus plays important roles in bacterial stress response, swimming motility, biofilm formation, cytotoxicity, virulence, and gene regulation.
MICROBIAL PATHOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Ruida Wang, Jiaqi Zhao, Lei Chen, Jiang Ye, Haizhen Wu, Huizhan Zhang
Summary: In this study, it was identified that LcbR1 functions as a repressor for lincomycin biosynthesis in Streptomyces lincolnensis. LcbR1 suppresses the transcription of lcbR1 itself and SLINC_1596, resulting in an increase in lincomycin production. The expression of lcbR1 may be regulated by GlnR, SLCG_Lrp, and SLCG_2919.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yeonbum Kim, Jung-Hye Roe, Joo-Hong Park, Yong-Joon Cho, Kang-Lok Lee
Summary: CatR, a peroxide-sensing transcriptional repressor of Fur family, can de-repress the transcription of the catA gene encoding catalase upon peroxide stress in Streptomyces coelicolor. Transcriptomic analysis identified new members of the CatR regulon and a consensus sequence in the promoter region of catR gene and catA operon for direct regulation. Hydrogen peroxide affects the expression of iron uptake genes through DmdR1 and CatR-regulated VtlA protein may play a role in reducing intracellular iron to prevent oxidative stress.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ankita Nag, Sarika Mehra
Summary: Overexpression of efflux pumps, particularly SCO4121, in Streptomyces species contributes to multidrug resistance and increased tolerance against oxidative stress. The adjacent MarR regulator, SCO4122, positively regulates SCO4121 in response to various drugs and HOCl, highlighting novel mechanisms of antibiotic resistance in soil bacteria that may have implications for clinically important pathogens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Jia-Yi Li, Jun-Yu Liang, Zhao-Yuan Liu, Yue-Zhao Yi, Jing Zhao, Zhi-Yong Huang, Jun Chen
Summary: This study investigates a stable lasso peptide called Aborycin and demonstrates that integrating multiple copies of the gul gene into the chromosome of S. coelicolor hosts can significantly increase its production. Additionally, knocking out certain negative regulatory genes also leads to improved production. These findings provide important insights for enhancing antibiotic production in Streptomyces systems.
Article
Biochemistry & Molecular Biology
Armelle Vigouroux, Thibault Meyer, Anais Naretto, Pierre Legrand, Magali Aumont-Nicaise, Aurelie Di Cicco, Sebastien Renoud, Jeanne Dore, Daniel Levy, Ludovic Vial, Celine Lavire, Solange Morera
Summary: The species-specific region SpG8-1b plays a critical role in controlling the transition between rhizospheric and pathogenic lifestyles of the plant pathogen. The transcriptional regulator Atu1419, belonging to the VanR group of GntR superfamily, represses genes in the HCAs catabolic pathway and is regulated by the effector molecule N5,N10-methylenetetrahydrofolate. The study sheds light on the allosteric mechanism of transcription employed by this GntR repressor.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sebastiaan Werten, Paul Waack, Gottfried J. Palm, Marie-Joelle Virolle, Winfried Hinrichs
Summary: TetR/AcrR-like transcription regulators enable bacteria to sense and adapt to chemical compounds. This study characterizes SCO3201, an atypical member of the TetR/AcrR family. SCO3201 strongly represses antibiotic production and morphological development in Streptomyces coelicolor under conditions of overexpression. The crystal structures reveal that in the absence of a ligand, SCO3201 is unable to span the distance between the two half-sites of its operator. Interaction with the ligand induces a major structural rearrangement, increasing the interdomain distance and potentially facilitating dimer-operator interaction. This 'reverse' induction mechanism expands the regulatory repertoire of the TetR/AcrR family and explains SCO3201's impact on antibiotic production and sporulation in S. coelicolor.
Review
Biotechnology & Applied Microbiology
Hee-Ju Nah, Jihee Park, Sisun Choi, Eung-Soo Kim
Summary: Streptomyces species are soil-dwelling bacteria that produce a large number of pharmaceutically valuable secondary metabolites, but the biosynthesis of these metabolites is often low due to tightly controlled regulatory networks. WblA, a global regulator of secondary metabolite biosynthesis, has been identified in several Streptomyces species and has significantly increased the productivity of these metabolites.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Maral Tsevelkhoroloo, So Heon Shim, Chang-Ro Lee, Soon-Kwang Hong, Young-Soo Hong
Summary: This study elucidated the role of DagR in regulating the expression of three agarase genes in S. coelicolor A3(2) by developing a dagR-deletion mutant. The Delta sco3485 mutant showed increased mRNA levels of agarolytic pathway genes and higher agarase production, indicating that DagR encodes a repressor that inhibits gene expression at the transcriptional level. Unique inhibition of DNA-binding activity of DagR by AHG was observed in this study.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Marieta M. Avramova, Clare E. M. Stevenson, Govind Chandra, Neil A. Holmes, Matthew J. Bush, Kim C. Findlay, Mark J. Buttner
Summary: To facilitate reproduction and dispersion, the filamentous bacterium Streptomyces forms specialized structures called aerial hyphae. These structures are controlled by the bld genes, many of which encode transcription factors. Among them, the function of BldB has remained elusive. This study investigates the global effects of deleting bldB and identifies target genes that are significantly upregulated. The study also characterizes a transcription factor, WhiJ9, which mediates the upregulation of one of these target genes.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yi Hao, Wenshuai Liu, Xingwang Li, Ying Wen
Summary: This study investigates the regulatory mechanism of AfsR and AfsS in Streptomyces species and their roles in antibiotic production and development. The results demonstrate that AfsR and AfsS negatively regulate avermectin production and morphological development and their interaction enhances the binding affinity of AfsR to target promoters.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ankita Nag, Sarika Mehra
Summary: This study identifies the role of a MFS transporter, SCO3366, from Streptomyces coelicolor in multidrug resistance and oxidative stress tolerance. SCO3366 provides resistance to structurally unrelated drugs and increases tolerance towards oxidative stress. The pump is directly regulated by the TetR regulator SCO3367 in a negative manner and the repression is relieved in the presence of different compounds recognized as substrates of SCO3366.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Bailey Bonet, Yein Ra, Luis M. Cantu Morin, Javier Soto Bustos, Jonathan Livny, Matthew F. Traxler
Summary: Interactions between different species of bacteria can trigger the production of specialized metabolites, such as antibiotics, and this process is regulated at the genetic level by conservon systems, which utilize small GTPases as a key component of their signaling cascades.
Article
Microbiology
Ting Lu, Xiaohua Wu, Qun Cao, Yongzhen Xia, Luying Xun, Huaiwei Liu
Summary: This study reveals a new posttranslational regulation mechanism involving sulfane sulfur that stimulates antibiotic production in Streptomyces, providing insights into the regulation of biosynthetic gene clusters in this genus.
Article
Chemistry, Medicinal
Xiao Xu, Falei Zhang, Luning Zhou, Yimin Chang, Qian Che, Tianjiao Zhu, Dehai Li, Guojian Zhang
Summary: In this study, six angucyclines, including three previously unreported compounds, were isolated from Streptomyces sp. XS-16 by overexpressing the native global regulator of SCrp. The structures were characterized using NMR and spectrometry analysis, as well as assisted by ECD calculations. All compounds were evaluated for their antitumor and antimicrobial activities, and compound 1 showed varying inhibitory activities against different tumor cell lines with IC50 values ranging from 0.32 to 5.33 μM.
Article
Biochemical Research Methods
Stefan Klingl, Sina Kordes, Benedikt Schmid, Roman G. Gerlach, Michael Hensel, Yves A. Muller
PROTEIN EXPRESSION AND PURIFICATION
(2020)
Review
Virology
Manfred Marschall, Sigrun Haege, Marcus Conrad, Sewar Alkhashrom, Jintawee Kicuntod, Johannes Schweininger, Mark Kriegel, Josephine Loesing, Julia Tillmanns, Frank Neipel, Jutta Eichler, Yves A. Muller, Heinrich Sticht
Article
Cell Biology
Anna Bochicchio, Astrid F. Brandner, Oskar Engberg, Daniel Huster, Rainer A. Boeckmann
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Simon Hanio, Jonas Schlauersbach, Bettina Lenz, Franziska Spiegel, Rainer A. Boeckmann, Ralf Schweins, Ivo Nischang, Ulrich S. Schubert, Sebastian Endres, Ann-Christin Poeppler, Ferdinand P. Brandl, Theo M. Smit, Karl Kolter, Lorenz Meinel
Summary: The study investigated the impact of Perphenazine concentrations on bile colloids using various techniques, revealing that Perphenazine affects the structure and thermodynamics of the colloids in a concentration-dependent manner. Despite inducing dynamic changes in the colloids, they effectively maintained stable levels of free Perphenazine throughout the tested concentration range.
Article
Biochemical Research Methods
Florian C. Grau, Andreas Burkovski, Yves A. Muller
Summary: P-II proteins are involved in regulating nitrogen/carbon balance in bacteria, archaea, plants, and algae through signal transduction modulated by effector molecules and post-translational modifications. The crystal structures of GlnK from Corynebacterium glutamicum, both adenylylated and unmodified, provide insights into the structural implications of covalent attachment of an AMP moiety to the T-loop. Adenylylation of GlnK does not alter the overall fold and does not interfere with the formation of functionally important T-loop conformations.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Chemistry, Applied
Marie-Louise Heymich, Ulrike Friedlein, Marius Trollmann, Karin Schwaiger, Rainer A. Boeckmann, Monika Pischetsrieder
Summary: By hydrolyzing the storage protein legumin extracted from chickpea, novel antimicrobial peptides Leg1 and Leg2 were developed, exhibiting high antimicrobial activity against a wide range of bacteria, including pathogens and antibiotic-resistant strains. These peptides showed bactericidal activity and were 10-1,000-fold more active compared to conventional food preservatives.
Article
Microbiology
Johannes Schweininger, Myriam Scherer, Franziska Rothemund, Eva-Maria Schilling, Sonja Worz, Thomas Stamminger, Yves A. Muller
Summary: Cytomegaloviruses have evolved a species-specific IE1 protein tertiary structure to effectively bind and inactivate the important cellular restriction factor PML, forming a barrier to cross-species transmission. The highly conserved fold of IE1 maximizes the efficacy of PML targeting in a species-specific manner, illustrating the co-evolution of viruses and their hosts.
Article
Biophysics
Christoph Kluge, Matthias Poehnl, Rainer A. Boeckmann
Summary: The curvature of cellular membranes plays a crucial role in targeting and sorting membrane-associated proteins. In this study, molecular dynamics simulations were used to determine the induced spontaneous curvature values of potassium channel and water channel proteins. The simulations revealed that the potassium channel induces positive membrane curvature, while the water channel induces negative curvature. Protein-lipid interaction analysis showed that the potassium channel shapes the surrounding membrane through structural determinants, while the water channel has a minimal effect on the membrane environment due to its wedge-like shape.
BIOPHYSICAL JOURNAL
(2022)
Article
Biophysics
Marius F. W. Trollmann, Rainer A. Boeckmann
Summary: This study provides an atomistic view of the structure, organization, and properties of the Comirnaty vaccine lipid nanoparticles (LNPs). The simulations reveal that the LNP core is oil-like at physiological pH, composed of aminolipids and cholesterol, while at low pH, it spontaneously forms lipid bilayers with high elasticity.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Physical
Matthias Poehnl, Christoph Kluge, Rainer A. Boeckmann
Summary: The lipid bicelle model can be used to study asymmetric lipid bilayer systems and allows for the formation of local spontaneous curvature induced by lipids or proteins. This system also has unbiased thermal fluctuations compared to standard bilayer systems.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2023)
Meeting Abstract
Immunology
Jennifer J. Luhr, Nils Alex, Lukas Amon, Martin Kraeter, Marketa Kubankova, Erdinc Sezgin, Christian H. K. Lehmann, Lukas Heger, Gordon F. Heidkamp, Ana Suncana Smith, Vasily Zaburdaev, Rainer A. Boeckmann, Ilya Levental, Michael L. Dustin, Christian Eggeling, Jochen Guck, Diana Dudziak
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Biophysics
Astrid F. Brandner, Anna Bochicchio, Daniel Huster, Rainer A. Boeckmann
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Meeting Abstract
Biophysics
Matthias Poehnl, Falk Nimmerjahn, Rainer A. Boeckmann
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Meeting Abstract
Biophysics
Matthias Poehnl, Rainer A. Boeckmann
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Meeting Abstract
Biophysics
Marius Trollmann, Rainer A. Boeckmann
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)