Review
Cell Biology
Tae-Kyung Hong, Jae-Hoon Song, So-Been Lee, Jeong-Tae Do
Summary: Assisted reproductive technologies (ARTs) have limitations in rectifying germ cell aplasia such as non-obstructive azoospermia (NOA) and oocyte maturation failure syndrome. In vitro gametogenesis shows promise in overcoming infertility, with research focusing on deriving male and female germ cells from pluripotent stem cells (PSCs). This technique not only helps understand the biological mechanism of gamete development but also offers insights into its application in infertility treatments.
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Cell & Tissue Engineering
Finn J. Hawkins, Shingo Suzuki, Mary Lou Beermann, Cristina Barilla, Ruobing Wang, Carlos Villacorta-Martin, Andrew Berical, J. C. Jean, Jake Le Suer, Taylor Matte, Chantelle Simone-Roach, Yang Tang, Thorsten M. Schlaeger, Ana M. Crane, Nadine Matthias, Sarah X. L. Huang, Scott H. Randell, Joshua Wu, Jason R. Spence, Gianni Carraro, Barry R. Stripp, Andras Rab, Eric J. Sorsher, Amjad Horani, Steven L. Brody, Brian R. Davis, Darrell N. Kotton
Summary: The directed differentiation of human iPSCs into airway basal cells (iBCs) demonstrates the functional characteristics of airway basal cells and can be used to model characteristic changes in airway diseases.
Article
Multidisciplinary Sciences
Yanxing Wei, Tianyu Wang, Lishi Ma, Yanqi Zhang, Yuan Zhao, Kathryn Lye, Lu Xiao, Chunlin Chen, Zhijian Wang, Yanlin Ma, Xiaohua Zhou, Fei Sun, Weili Li, Caroline Dunk, Siliang Li, Andras Nagy, Yanhong Yu, Guangjin Pan, Stephen J. Lye, Yongli Shan
Summary: This passage discusses the successful generation of trophoblast stem cells from primed human pluripotent stem cells, with BMP4 playing a significant role in the process. The derived TSCs are similar to blastocyst-derived hTSCs in various characteristics, and the chromatin accessibility dynamics and histone modifications involved in specifying hPSC-derived TSCs are defined. Additionally, knockout of H3K27 methyltransferases (EZH1/2) is shown to increase the efficiency of hTSC derivation from primed hPSCs.
Review
Engineering, Biomedical
Camilla Luni, Onelia Gagliano, Nicola Elvassore
Summary: An integrative approach combining microfluidic design and stem cell biology allows for the capture of spatial-temporal environmental changes related to epigenetic remodeling and morphogenetic processes, contributing to significant advances in stem cell engineering.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Rowan M. Karvas, Laurent David, Thorold W. Theunissen
Summary: Trophoblasts play critical roles in blastocyst implantation and maternal-fetal communication during pregnancy. However, our understanding of human trophoblast biology is limited due to the scarcity of first-trimester placental tissue and the inadequacy of animal models. Recent advancements in deriving human trophoblast stem cells from pluripotent and somatic cell sources provide exciting opportunities to study the molecular mechanisms of placental development and pregnancy-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Shuo Cao, Xinyue Gao, Fangyuan Liu, Yanglin Chen, Qin Na, Qiaoqiao Meng, Peng Shao, Chen Chen, Yongli Song, Baojiang Wu, Xihe Li, Siqin Bao
Summary: This study derived iPSCs from fibroblasts of a patient with acute myelitis and compared them to iPSCs from normal individuals. The patient-derived iPSCs showed attenuated potential for ectoderm differentiation, which was found to be related to the Wnt signaling pathway. This study provides a novel approach to investigating the pathogenesis of acute myelitis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Alberto Posabella, Andrea B. Alber, Hendrik J. Undeutsch, Raoul A. Droeser, Anthony N. Hollenberg, Laertis Ikonomou, Darrell N. Kotton
Summary: Stem cell-based therapies hold potential for reconstituting organ function, particularly in addressing hypothyroidism. Research has shown promise in engineering thyroid follicular epithelial cells to potentially cure the disease, but technical and biological challenges need to be overcome to achieve long-term cure through cell- and/or gene-based therapies.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Pinmou Zhu, Bohang Zhang, Ruiqi Sun, Jiachen Wang, Zhaode Liu, Xiaorui Liu, Min Yan, Yiqiang Cui, Jiahao Sha, Yan Yuan
Summary: In this study, researchers have successfully transformed human extended pluripotent stem cells (hEPSCs) into a novel intermediate pluripotent stem cell resembling embryonic days 8-9 (E8-E9) epiblasts. They have demonstrated that these intermediate stem cells possess the ability to differentiate into trophectoderm, primordial germ cells-like cells (PGCLC), endoderm, mesoderm, and neural ectoderm. Furthermore, these cells exhibit direct responsiveness to primordial germ cells (PGCs) specification and three germ layer differentiation signals.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Marta Cadenas-Martin, Francisco Arnalich-Montiel, Maria P. De Miguel
Summary: This study developed a protocol for deriving human limbal stem cells (LSCs) from adipose-derived mesenchymal stem cells (ADSCs) that is compatible with the standard carrier human amniotic membrane. The best protocol achieved optimal differentiation efficiency and will benefit patients with LSC deficiency, aiding in the restoration of vision.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Francesca Soncin, Robert Morey, Tony Bui, Daniela F. Requena, Virginia Chu Cheung, Sampada Kallol, Ryan Kittle, Madeline G. Jackson, Omar Farah, Jennifer Chousal, Morgan Meads, Donald Pizzo, Mariko Horii, Kathleen M. Fisch, Mana M. Parast
Summary: Functional trophoblast stem cells (TSCs) can be derived from primed human pluripotent stem cells (hPSCs) through a simple protocol, which can be applied to a widely available set of existing hPSCs, including induced pluripotent stem cells.
Correction
Cell & Tissue Engineering
Ran Zheng, Ting Geng, Dan-Ya Wu, Tianzhe Zhang, Hai-Nan He, Hai-Ning Du, Donghui Zhang, Yi-Liang Miao, Wei Jiang
Summary: A new method has been developed to convert human embryonic stem cells and induced pluripotent stem cells into EPSCs using chemicals, demonstrating extended pluripotency in terms of molecular features, chimeric ability, and transcriptome. Certain chemicals targeting glycolysis and histone methyltransferase have been identified to facilitate the conversion and maintenance of feeder-free EPSCs, providing insights for further research and applications in the field of pluripotency.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Cell Biology
V. K. Abdyyev, N. O. Dashenkova, E. B. Dashinimaev, E. A. Vorotelyak, A. V. Vasiliev
Summary: Human infertility is a complex disorder involving genetic, molecular, cellular, organ, and hormonal levels. New technology generating hPGCLCs from hiPSCs may offer insights into early germ cell development and provide solutions for infertility. Study on DS hPGCLCs could help understand human early germ cell development and related infertility disorders.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Nadezhda A. Evtushenko, Arkadii K. Beilin, Anastasiya Kosykh, Ekaterina A. Vorotelyak, Nadya G. Gurskaya
Summary: Epidermolysis bullosa simplex (EBS) is a group of genetic diseases caused by mutations in keratins, leading to changes in cellular pathology such as fragility of the intermediate filament network and basal layer cytolysis. Mutations in keratins can affect cellular signaling, cause abnormal cell migration, and trigger inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Alexandra L. Rippa, Elena V. Alpeeva, Andrey V. Vasiliev, Ekaterina A. Vorotelyak
Summary: Understanding the formation of secondary septa during pulmonary development and regeneration is crucial for therapeutic approaches to lung pathologies. Factors such as actomyosin cytoskeleton, retinoic acid signaling play important roles in alveologenesis, but the mechanistic context remains incomplete.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
L. Sh Izmailova, A. O. Gaidamaka, O. O. Serbina, E. A. Vorotelyak
Summary: Embryo implantation preparation is a complex process involving changes at the cellular and tissue levels in the endometrium of the uterus. Success of pregnancy relies on proper regulation of preimplantation changes and receptivity onset. Understanding the influence of steroid sex hormones on endometrial epithelium polarity and the interaction between estrogen and progesterone is crucial for therapeutic developments in infertility treatment.
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
Article
Physics, Applied
E. A. Shershunova, I. E. Rebrov, V. V. Mishchenko, A. Kryukov, E. A. Vorotelyak, O. S. Rogovaya
Summary: An electrophysical apparatus for combined electroporation and iontophoresis treatment of biological membranes is described, with estimates of cell viability provided in living tissue equivalents exposed to pulsed current with a dc offset. The values of electrophysical pulse characteristics compatible with cell viability in the living tissue equivalents have been determined, suggesting potential use for transportation of pharmaceutical compounds through an equivalent of the round window membrane of the cochlea. A technique of drug administration across biological barriers based on pulsed electric field equipment could be developed for drug delivery into the inner ear.
TECHNICAL PHYSICS LETTERS
(2021)
Article
Clinical Neurology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigoni, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
Summary: This study demonstrates that the pathogenic variant G2019S of leucine-rich repeat kinase 2 (LRRK2) impairs the function of Excitatory Amino Acid Transporter 2 (EAAT2), resulting in glutamate overload in the brain, which may contribute to neurodegeneration in Parkinson's disease (PD).
ACTA NEUROPATHOLOGICA
(2022)
Article
Developmental Biology
O. L. Cherkashina, A. V. Kosykh, E. I. Morgun, A. L. Rippa, A. A. Tsitrina, A. P. Oettinger, E. S. Chermnykh, E. P. Kalabusheva, E. A. Vorotelyak
Summary: Xenotransplantation of human skin to immunodeficient mice is a suitable model for studying regeneration mechanisms and has broad prospects in cell biology and regenerative medicine research. The expression and activation of YAP1 protein during the regeneration of human skin play a significant role.
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mariana V. Karimova, Inessa G. Gvazava, Ekaterina A. Vorotelyak
Summary: Researchers are developing methods to generate insulin-producing cells from stem cells, providing an unlimited source for diabetes treatment. While current methods can produce beta-like cells, they do not fully mature functionally compared to native beta cells. This paper reviews the development of various protocols, considers advantages, and proposes ways to improve them.
Article
Cell Biology
Alena Kashirina, Alena Gavrina, Artem Mozherov, Dmitriy Kozlov, Daria Kuznetsova, Ekaterina Vorotelyak, Elena Zagaynova, Ekaterina Kalabusheva, Aleksandra Kashina
Summary: This study investigated the metabolic status and pH of dermal spheroids created from dermal papilla cells differentiated from pluripotent stem cells and native dermal papilla cells using fluorescence microscopy and FLIM. The results showed that spheroids from pluripotent stem cells exhibited a more glycolytic phenotype and alkaline intra-cellular pH compared to spheroids from native dermal papilla cells. The metabolic activity of the spheroids was also found to be dependent on the source of the stem cells.
Article
Medicine, General & Internal
O. S. Rogovaya, E. Alpeeva, E. S. Ruchko, A. Eremeev, E. A. Vorotelyak
Summary: This study aimed to determine the shelf life of dermal equivalent (DE) and skin equivalent (SE) tissue-engineered constructs (TECs) stored in normal saline at room temperature. Results showed that the viability and metabolic activity of the cells on the surface of TECs decreased by more than 50% by days 3-4 of storage. Furthermore, the decrease was faster in DEs than in SEs.
BULLETIN OF RUSSIAN STATE MEDICAL UNIVERSITY
(2023)
Review
Biochemistry & Molecular Biology
Ekaterina P. P. Kalabusheva, Anastasia S. S. Shtompel, Alexandra L. L. Rippa, Sergey V. V. Ulianov, Sergey V. V. Razin, Ekaterina A. A. Vorotelyak
Summary: Keratins are specific intermediate filament-forming proteins found in epithelial cells. Their expression profiles change in different biological conditions, such as cell differentiation, injury, and malignant transformation. The tight regulation of keratin expression suggests the presence of complex regulatory mechanisms at the genomic level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Oksana I. I. Sutyagina, Arkadii K. K. Beilin, Ekaterina A. A. Vorotelyak, Andrey V. V. Vasiliev
Summary: Immortalization is a promising method for obtaining cellular material for cell therapy or bio-artificial organs. However, the reversibility of immortalization is uncertain and may cause unwanted changes in cells. This review discusses various approaches to reversible immortalization, emphasizing their advantages and disadvantages in terms of biosafety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigono, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
I. G. Gvazava, M. V. Karimova, A. V. Vasiliev, E. A. Vorotelyak
Summary: This review article provides an overview of the commonly used animal models for studying the pathophysiology and therapy of type 2 diabetes mellitus (T2DM), discussing their advantages and disadvantages as well as the factors to consider when selecting a suitable model.
Meeting Abstract
Cell & Tissue Engineering
Alena Kashirina, Aleksandra Kashina, Erdem Dashinimaev, Ekaterina Vorotelyak, Alena Gavrina, Emil Kryukov, Vadim Elagin, Elena Zagaynova
TISSUE ENGINEERING PART A
(2022)