Article
Biochemistry & Molecular Biology
Hui-Jie Jia, Min Zhou, Manoj Kumar Vashisth, Jing Xia, Hui Hua, Qian-long Dai, Shi-Rui Bai, Qi Zhao, Xiao-bo Wang, Yi-ling Shi
Summary: This study evaluates the impact of trifluridine on cellular senescence and investigates its possible mechanism. The findings reveal that trifluridine induces senescence in endothelial cells, which is closely related to the inhibition of autophagy and activation of the mTOR signaling pathway. This study provides a new strategy for establishing autophagy or aging models and offers a new theoretical basis for the clinical application of trifluridine.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Saad Mustafa, Jogendra Singh Pawar, Ilora Ghosh
Summary: The study found that Fucoidan induces cytotoxicity in HeLa cells through the generation of ROS and mitochondrial superoxide, as well as ATP loss. Additionally, Fucoidan disrupts HDAC function, leading to autophagy and cellular senescence.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Medicine, Research & Experimental
Kensei Taguchi, Bertha C. Elias, Sho Sugahara, Snehal Sant, Benjamin S. Freedman, Sushrut S. Waikar, Ambra Pozzi, Roy Zent, Raymond C. Harris, Samir M. Parikh, Craig R. Brooks
Summary: Acute kidney injury (AKI) can lead to chronic kidney disease (CKD) through maladaptive repair of proximal tubule cells (PTCs), involving dedifferentiation and profibrotic cytokine secretion. Cyclin G1 (CG1) and cyclin-dependent kinase 5 (CDK5) are key factors in maladaptive dedifferentiation and could be therapeutic targets for CKD.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cell Biology
Xiao Lu, Jiao Zhang, Yan-Qi Li, Quan-Xing Liu, Dong Zhou, Xu-Feng Deng, Yuan Qiu, Qian Chen, Man-Yuan Li, Xiao-Qing Liu, Ji-Gang Dai, Hong Zheng
Summary: This study found that CSP in Plasmodium can enhance the anti-tumor effect of EGFR-TKIs by inhibiting autophagy. The results suggest that autophagy plays an important role in the development of resistance to EGFR-TKIs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Guangyan Kan, Ziyang Wang, Chunjie Sheng, Chen Yao, Yizhi Mao, Shuai Chen
Summary: The study revealed that high expression of DKC1 in LUAD indicates poor prognosis, and downregulation of DKC1 can induce telomere-related cell senescence and apoptosis. This suggests that DKC1 could potentially serve as a candidate diagnostic biomarker and therapeutic target for LUAD.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Chemistry, Medicinal
Zheng Wei, Tingting Li, Yanping Sun, Huilin Su, Yuanning Zeng, Qiuhong Wang, Haixue Kuang
Summary: DTA induces autophagy in HaCaT cells, leading to cell senescence and cell cycle arrest, while negatively regulating inflammation by activating autophagy. This may be one of the mechanisms underlying the action of Datura metel L. preparation used for the treatment of psoriasis.
PHYTOTHERAPY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jian-Xiang Liu, Dao-Lei Cui, Dan-Lei Yang, Jing-Ya Li, Zi-Yue Yang, Jin-Zhou Su, Cai-Xia Ren, You-Ya Niu, Ping Xiang
Summary: Tris (1-chloro-2-propyl) phosphate (TCPP) is a commonly detected organophosphorus flame retardant in the environment. This study investigated the detrimental effects of TCPP on human skin keratinocytes and found that it decreased cell viability, induced reactive oxygen species generation and DNA damage, arrested the cell cycle at the G1 phase, and caused cellular senescence through the p53-dependent DNA damage signal pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Kunihiko Futami, Kako Aoyama, Kazuki Fukuda, Masashi Maita, Takayuki Katagiri
Summary: The study showed that DNA demethylation with 5-Aza-dC can induce senescence in telomere-independent fish cell line EPC. Overexpression of HRas gene contributes to early senescence in EPC cells, but may not be sufficient for full senescence. The limited mechanism of Ras-induced senescence may play a role in the senescence-resistance of fish cell lines.
Article
Medicine, Research & Experimental
Jing Xia, Yu Zhou, Siyue He, Manoj Kumar Vashisth, Huijie Jia, Qianlong Dai, Yufen He, Xiaobo Wang
Summary: In this study, it was found that amonafide induces cellular senescence in human umbilical vein endothelial cell (HUVEC) lines by inhibiting autophagy and activating the mTOR pathway.
DISCOVERY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Tzu-Jung Yu, Jen-Yang Tang, Jun-Ping Shiau, Ming-Feng Hou, Chia-Hung Yen, Fu Ou-Yang, Chung-Yi Chen, Hsueh-Wei Chang
Summary: Gin A, a compound isolated from ginger, has been found to inhibit proliferation and induce senescence of breast cancer cells through oxidative stress. It decreases cellular ATP content and cell viability, and delays the G2/M response in breast cancer cells. Additionally, Gin A induces oxidative stress and senescence responses by increasing reactive oxygen species, mitochondrial superoxide, and beta-galactosidase activity, and upregulates senescence-associated gene expressions. Gin A also induces DNA damage responses. These findings suggest the potential anticancer effects of Gin A on breast cancer cells.
Article
Endocrinology & Metabolism
Davis A. Englund, Alyssa Jolliffe, Zaira Aversa, Xu Zhang, Ines Sturmlechner, Ayumi E. Sakamoto, Julianna D. Zeidler, Gina M. Warner, Colton McNinch, Thomas A. White, Eduardo N. Chini, Darren J. Baker, Jan M. van Deursen, Nathan K. LeBrasseur
Summary: Recent research has found associations between elevated levels of p21, the accumulation of senescent cells, and skeletal muscle dysfunction in mice and humans. By using a mouse model with p21 overexpression, the study investigated the role of p21 in cellular senescence and pathological features in skeletal muscle. The findings showed that p21 induced several characteristic features of cellular senescence in skeletal muscle, including altered gene expression, DNA damage, mitochondrial dysfunction, and the senescence-associated secretory phenotype. Additionally, the p21OE mice exhibited skeletal muscle pathology, such as atrophy, fibrosis, and impaired physical function, highlighting p21 as a driver of cellular senescence and a new source of skeletal muscle loss and dysfunction.
MOLECULAR METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Hui Hu, Xiao-Wei Zhang, Lin Li, Ming-Ning Hu, Wen-Qian Hu, Jing-Ying Zhang, Xiao-Kang Miao, Wen-Le Yang, Ling-Yun Mou
Summary: Non-small cell lung cancer (NSCLC) is the most common cancer in the world, and Gefitinib has been shown to be effective in treating NSCLC. However, innate and acquired resistance present challenges in therapy. Inhibition of autophagy is considered a promising strategy to overcome resistance.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Dekun Wang, Lichun Kang, Chuan'ai Chen, Jiasen Guo, Lingfang Du, Donghui Zhou, Gang Li, Yuying Zhang, Xue Mi, Mianzhi Zhang, Shuxia Liu, Xiaoyue Tan
Summary: The downregulation of legumain in the renal tubules during aging is associated with the development of renal interstitial fibrosis. Legumain deficiency induces tubular senescence and increases the secretion of profibrotic cytokines, leading to fibroblast activation and fibrosis. Legumain downregulation also impairs lysosomal homeostasis and mitophagy, resulting in mitochondrial ROS accumulation and tubular senescence. Supplementing exosomal legumain can ameliorate fibronectin and collagen I production.
Article
Medicine, Research & Experimental
Jiahui Si, Yuanyuan Ma, Chao Lv, Yang Hong, Hongyu Tan, Yue Yang
Summary: The overexpression of long non-coding RNA HIF1A-AS2 in lung adenocarcinoma (LUAD) patients is associated with tumor progression and resistance to osimertinib. Through targeting the miR-146b5p/IL-6/STAT3 axis, HIF1A-AS2 functions as an oncogenic factor in LUAD cells and may serve as a potential prognostic indicator for patient outcomes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Yu Wang, Ran Zuo, Gengwei Huo, Zhiqiang Han, Yuchao He, Yi Luo, Liwei Chen, Guangtao Li, Jinfang Cui, Fuyi Zhu, Ping Yue, Dongqi Yuan, Yi Sun, Zhaoyue Li, Peng Chen, Hua Guo
Summary: Numerous studies have demonstrated the crucial involvement of actin cytoskeleton in cancer initiation and progression. As an actin-binding protein, Twinfilin1 (TWF1) is known to regulate cytoskeleton-related functions. However, the expression and function of TWF1 in human tumors, particularly in lung adenocarcinoma (LUAD), remain poorly understood.
Article
Medicine, General & Internal
Chia -Ter Chao, Chiao-Lin Tsai, Mong-Wei Lin, Chih-Wei Yang, Chao -Chi Ho, Huey -Ling Chen, Chiun Hsu, Bor-Ching Sheu
Summary: Digital problem-based learning (PBL) was originally introduced to improve student engagement and flexibility, but its mandatory use during the COVID-19 period accelerated changes in medical education. This article provides insights and recommendations for implementing digital PBL curricula based on experiences from National Taiwan University College of Medicine, aiming to promote the widespread adoption of digital PBL.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2022)
Article
Medicine, General & Internal
Ching-Kai Lin, Hung-Jen Fan, Zong-Han Yao, Yen-Ting Lin, Yueh-Feng Wen, Shang-Gin Wu, Chao-Chi Ho
Summary: CBCT-AF enhances the navigation success rate of EBUS-TBB for PPLs, especially with small lesions, and improves diagnostic yield, while maintaining a similar complication rate compared to traditional methods.
Article
Psychology, Clinical
Fei-Hsiu Hsiao, Zhong-Zhe Lin, Feng-Ying Huang, Yu-Ming Lai, Yu-Ting Chen, Jin-Yuan Shih, Chao-Chi Ho, Chong-Jen Yu, Chia-Chen Hsieh
Summary: The study showed that integrating mindfulness skills with BMS empowerment strategies could effectively improve symptom distress in lung cancer patients by enhancing mindfulness and regulating depressive symptoms.
Article
Microbiology
Ching-Kai Lin, Hung-Jen Fan, Kai-Lun Yu, Lih-Yu Chang, Yueh-Feng Wen, Li-Ta Keng, Chao-Chi Ho
Summary: This study verified the effectiveness and safety of endobronchial ultrasound-guided transbronchial biopsy (EBUS-TBB) in diagnosing sputum smear-negative pulmonary TB. The EBUS echoic feature was found to be a predictor of the positive TB culture rate in pulmonary TB. However, tissue culture via EBUS-TBB had little effect in improving the positive TB culture rate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Education & Educational Research
Chiao-Ling Tsai, Yen-Lin Chiu, Chia-Ter Chao, Mong-Wei Lin, Chao-Chi Ho, Huey-Ling Chen, Bor-Ching Sheu, Chiun Hsu, Chih-Wei Yang
Summary: This study evaluated the effect of tutor shadowing on the teaching skills of novice problem-based learning (PBL) tutors and developed a tutoring-skill scale (TS-scale). The results showed that tutor shadowing significantly improved the teaching confidence of novice PBL tutors across three key domains of tutoring competencies. The TS-scale developed in this study can be used in future research on faculty development design.
BMC MEDICAL EDUCATION
(2022)
Article
Respiratory System
Shih-Yu Chen, Yao-Wen Kuo, Chao-Chi Ho, Huey-Dong Wu, Hao-Chien Wang
Summary: Vacuum bottle plus non-tunneled catheter drainage is a safe and efficient procedure for managing iatrogenic pneumothorax larger than 15%. It can be considered as an alternative treatment for stable post-procedural pneumothorax.
BMC PULMONARY MEDICINE
(2022)
Article
Education & Educational Research
Chia-Ter Chao, Yen-Lin Chiu, Chiao-Ling Tsai, Mong-Wei Lin, Chih-Wei Yang, Chao-Chi Ho, Yen-Yuan Chen, Chiun Hsu, Huey-Ling Chen
Summary: Team dynamics and facilitator support play important roles in teamwork satisfaction in both conventional and digital small group tutorials, with the role of facilitator support becoming less obvious in digital settings.
BMC MEDICAL EDUCATION
(2022)
Article
Oncology
Chun-Ta Huang, Chih-An Lin, Te-Jen Su, Ching-Yao Yang, Tzu-Hsiu Tsai, Chia-Lin Hsu, Wei-Yu Liao, Kuan-Yu Chen, Chao-Chi Ho, Chong-Jen Yu
Summary: This study investigates the utility of droplet digital PCR (ddPCR) for detection of the T790M mutation in plasma circulating tumor DNA (ctDNA) and explores its impact on prognosis. The results show that ddPCR can effectively detect the T790M mutation and predict disease progression and patient survival in advance. Therefore, the plasma-based detection technology using ddPCR is of significant importance for the treatment and prognosis of EGFR-mutant non-small cell lung cancer.
Letter
Infectious Diseases
Meng-Rui Lee, Hung-Ling Huang, Wei-Chang Huang, Shang-Yu Wu, Pang-Chun Liu, Jui-Ching Wu, Meng-Hsuan Cheng, Chau-Chyun Sheu, Kea-Tiong Tang, Jann-Yuan Wang, Chao-Chi Ho, Jin-Yuan Shih, Inn-Wen Chong
JOURNAL OF INFECTION
(2023)
Article
Medicine, General & Internal
Ming-Fang Wu, Ya-Hsuan Chang, Hsuan-Yu Chen, Chao-Chi Ho, Huei-Wen Chen
Summary: Osimertinib is effective for lung adenocarcinoma patients with EGFR mutations, but combining it with immune checkpoint inhibitors increases the risk of interstitial lung disease. This study investigates the interaction between dendritic cells and osimertinib and suggests that osimertinib therapy may activate dendritic cell functions, potentially leading to increased immune toxicity when combined with immunotherapy.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2023)
Article
Oncology
Sheng-Kai Liang, Chang-Wei Wu, Ching- Chang, Li-Ta Keng, Meng-Rui Lee, Jann-Yuan Wang, Jen-Chung Ko, Wei-Yu Liao, Kuan-Yu Chen, Chao-Chi Ho, Jin-Yuan Shih, Chong-Jen Yu
Summary: This study compared the effectiveness of adjuvant oral UFT and intravenous chemotherapy in early-stage NSCLC patients after surgery and found that both treatments had comparable clinical outcomes.
Article
Respiratory System
Kai-Lun Yu, Yi-Shiuan Tseng, Han-Ching Yang, Chia-Jung Liu, Po-Chih Kuo, Meng-Rui Lee, Chun-Da Huang, Lu-Cheng Kuo, Jann-Yuan Wang, Chao-Chi Ho, Jin-Yuan Shih, Chong-Jen Yu
Summary: This study developed a CNN to differentiate between malignant and benign tumors in rEBUS images. The results demonstrate the feasibility of the proposed CNN-based algorithm in differentiating between malignant and benign lesions in rEBUS images.
BMJ OPEN RESPIRATORY RESEARCH
(2023)
Article
Oncology
Chia-Ling Chang, Min-Shu Hsieh, Jin-Yuan Shih, Yi-Hsuan Lee, Wei-Yu Liao, Chia-Lin Hsu, Ching-Yao Yang, Kuan-Yu Chen, Jih-Hsiang Lee, Chao-Chi Ho, Tzu-Hsiu Tsai, James Chih-Hsin Yang, Chong-Jen Yu
Summary: This study investigated the treatment patterns and outcomes of advanced non-small-cell lung cancer with spindle cell and/or giant cell carcinoma (NSCLCsg). The results showed that immune checkpoint inhibitors (ICI) may prolong the overall survival (OS) of patients with advanced NSCLCsg. This finding has important clinical implications for the treatment of NSCLCsg patients.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2022)
Article
Oncology
Ming-Fang Wu, Chih-An Lin, Tzu-Hang Yuan, Hsiang-Yuan Yeh, Sheng-Fang Su, Chin-Lin Guo, Gee-Chen Chang, Ker-Chau Li, Chao-Chi Ho, Huei-Wen Chen
Summary: The study identified the M1/M2 spectrum and plasticity of MPE-Mϕ, and described a two-gene M1/M2 signature that can predict outcomes of late-stage lung cancer patients. Furthermore, re-educating MPE-Mϕ towards anti-cancer M1 macrophages using clinically applicable strategies may overcome tumor immune escape and benefit anti-cancer therapies.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)