Article
Biochemistry & Molecular Biology
Asad Ullah, Xian Yin, Fenghuan Wang, Bo Xu, Zulfiqar Ali Mirani, Baocai Xu, Malik Wajid Hussain Chan, Amjad Ali, Muhammad Usman, Nawazish Ali, Muhammad Naveed
Summary: Selenium nanoparticles have minor toxicity, superior reactivity, and excellent bioavailability compared to other forms, and can be produced using a biosynthetic approach. These nanoparticles showed good antioxidant and antibacterial activity, making them potential candidates for biomedical and nutritional applications.
Article
Energy & Fuels
Bian Ziwei, Wu Hanning, Wei Lusha, Zhi Zena, Wu Yifei
Summary: This study identified eight bacterial strains from the low-permeability reservoir in the Ordos Basin that can produce biosurfactants and reduce viscosity. These strains can emulsify oil, tolerate different temperatures, salinity, and pH, and effectively degrade certain chemicals in oil. Additionally, they can reduce oil viscosity. The findings have important implications for the application of microbial-enhanced oil recovery in low-permeability oil fields.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Md Samiul Islam, Gamarelanbia Mohamed, Shakil Ahmed Polash, Md Amit Hasan, Razia Sultana, Noshin Saiara, Wubei Dong
Summary: Plants in natural ecosystems face threats from microbial diseases, but have developed a strong immune system to combat infections. Antimicrobial peptides (AMPs) in plants are environmentally friendly, anti-pathogenic, and safe for humans. A new isolation technique using high-throughput cDNA libraries and the Bacillus subtilis expression system allows for large-scale screening of unique or homologous AMPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Mei-yan Wu, Wen-wei Li, Graham Christie, Peter Setlow, Yong-qing Li
Summary: Using Raman tweezers and live-cell phase-contrast microscopy, this study characterized the lysis process of individual Bacillus subtilis cells carrying PBSX prophage during spores' germination, outgrowth, and vegetative growth. The findings showed that nucleic acid intensities in induced cultures decreased rapidly after prophage induction, with a delay before cell burst, and the latent time and burst time varied in cell populations under different culture conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jiaxin Zhang, Md. Samiul Islam, Jieyu Wang, Yang Zhao, Wubei Dong
Summary: In this study, two antimicrobial peptides (CgR2150 and CgR3101) with broad-spectrum antibacterial activities were identified from endophytes isolated from potato tubers. These peptides showed excellent stability and could significantly inhibit the growth of various bacteria and fungi. Scanning electron microscopy revealed that these peptides disrupt bacterial cell membranes. The findings suggest that CgR2150 and CgR3101 have potential as new antibacterial agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Elizabeth T. Montano, Jason F. Nideffer, Lauren Brumage, Marcella Erb, Julia Busch, Lynley Fernandez, Alan I. Derman, John Paul Davis, Elena Estrada, Sharon Fu, Danielle Le, Aishwarya Vuppala, Cassidy Tran, Elaine Luterstein, Shivani Lakkaraju, Sriya Panchagnula, Caroline Ren, Jennifer Doan, Sharon Tran, Jamielyn Soriano, Yuya Fujita, Pranathi Gutala, Quinn Fujii, Minda Lee, Anthony Bui, Carleen Villarreal, Samuel R. Shing, Sean Kim, Danielle Freeman, Vipula Racha, Alicia Ho, Prianka Kumar, Kian Falah, Thomas Dawson, Eray Enustun, Amy Prichard, Ana Gomez, Kanika Khanna, Shelly Trigg, Kit Pogliano, Joe Pogliano
Summary: The study identified novel gene clusters in Streptomyces strains that have the potential to produce new antibiotics. Additionally, it isolated and analyzed bacteriophages with antibacterial activity, as well as predicted the host ranges of these phages based on the CRISPR-Cas systems present in the strains.
Article
Biotechnology & Applied Microbiology
Huajun Zhu, Shenglian Wu, Shaojun Tang, Jun Xu, Yuelin He, Zuohua Ren, Erming Liu
Summary: In this study, cyclic lipopeptides extracted from Bacillus subtilis strain JN005 exhibited inhibition activity against the rice phytopathogenic fungus Magnaporthe oryzae. These isolated cyclic lipopeptides were tolerant to high temperature, extreme acidity, extreme alkalinity, and ultraviolet radiation. The growth of the mycelium and conidia of M. oryzae was abnormal after treatment with the cyclic lipopeptides.
BIOLOGICAL CONTROL
(2023)
Article
Biochemistry & Molecular Biology
Sumaia Akhter, Mubarak Ahmad Khan, Shobuz Mahmud, Suchi Parvin Biki, Md. Shamsuzzoha, S. M. Kamrul Hasan, Maruf Ahmed
Summary: In this study, bacterial nanocellulose and polyhydroxyalkanoate biopolymers were extracted and synthesized into biopolymer films with improved properties. The films exhibited higher crystalline properties, increased thickness, tensile strength, and elongation, as well as decreased solubility and swelling degree compared to the biopolymer-free film. The findings suggest that the BNC and PHA biopolymer-based films have potential applications in sustainable packaging.
PROCESS BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Angela Maria Catania, Tiziana Civera, Pierluigi Aldo Di Ciccio, Maria Ausilia Grassi, Patrizia Morra, Alessandra Dalmasso
Summary: Processed cheeses from an Italian dairy plant were found to contain Bacillus strains in the initial production hours. Genetic analysis revealed different sequence types for Bacillus cereus strains and identification of a new sequence type for Bacillus subtilis strains. All isolates showed biofilm-forming ability, suggesting the presence of a recurring Bacillus population.
Article
Microbiology
Sonal Chaudhary, Ajit Varma, Manabendra Mandal, Ram Prasad, Shalini Porwal
Summary: This study isolated a highly productive laccase-producing bacteria, Bhargavaea bejingensis, from soil samples collected from the paper and pulp industry. The extracellular and intracellular laccase activities of the bacteria after 24 h incubation were 1.41 U/mL and 4.95 U/mL, respectively. The laccase-encoding gene of this bacteria was sequenced and bioinformatically characterized to be structurally and sequentially homologous to the CotA protein of Bacillus subtilis. The laccase produced by B. bejingensis was classified as a three-domain laccase with several copper-binding residues.
CURRENT MICROBIOLOGY
(2023)
Article
Food Science & Technology
Manami Kimijima, Kengo Mandokoro, Yuki Ichikawa, Mizuki Tokumaru, Naoki Narisawa, Fumio Takenaga
Summary: This study evaluated the genetic and enzymatic characteristics of Bacillus subtilis obtained from commercial honey and found that these strains are suitable for natto fermentation. The results indicate that honey is a potential source of B. subtilis natto for the food industry.
FOOD SCIENCE AND TECHNOLOGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ramamoorthy Sathishkumar, Ramachandran Kannan, Sekar Jinendiran, Natesan Sivakumar, Gopal Selvakumar, Rajaram Shyamkumar
Summary: In this study, the production of exopolysaccharide (EPS) by a potential endosymbiotic bacterium associated with sponge was optimized using response surface methodology. The purified EPS exhibited strong antioxidant activity and moderate anticoagulant efficacy, with the potential applications in food and pharmaceutical industries highlighted for the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Horticulture
Yao Ma, Siwen Zhang, Jiawei Hu, Shiyong Chen, Juanjuan Wang
Summary: With excessive use of nitrogen fertilizer, the accumulation of nitrate in soil and vegetables has become a serious problem. This study found that the combination of Bacillus subtilis Strain11 and Molybdenum can effectively promote the growth of Chinese cabbage and reduce soil nitrate content.
Article
Biochemistry & Molecular Biology
Aniruddha Tewary, Ranjit Kumar Prajapati, Jayanta Mukhopadhyay
Summary: The C-terminal domain of the α subunit of RNA polymerase is targeted by transcriptional regulators in the activation of transcription. In bacteria, the regulator binds to DNA at different sites and interacts with the α CTD to stabilize the RNA polymerase at the promoter or form a pre-recruitment complex. In B. subtilis, the binding of RNA polymerase at the promoter stabilizes the transcriptional regulator and facilitates open complex formation. Two residues of the α CTD are essential for this process.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mengyao Hao, Aimin Huang, Bingjie Li, Yu Xin, Liang Zhang, ZhengHua Gu, Haiyan Sun, Youran Li, Guiyang Shi
Summary: In this study, a laccase-like gene from Thermomicrobium roseum DSM 5159 (TrLac-like) was expressed in Bacillus subtilis WB600. TrLac-like showed optimal temperature and pH of 50 degrees C and 6.0, respectively. Amino acid substitutions within 5 angstrom of the inosine ligand were simulated to improve catalytic efficiency.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Environmental Sciences
Min Zhou, Xianni Yang, Renhua Sun, Xiaozhi Wang, Weiqin Yin, Shengsen Wang, Jun Wang
Summary: The study demonstrated that biochars derived from cellulose and hemicellulose have higher Cr(VI) removal capacity and are more conducive to electron transfer for ZVI, while biochars derived from lignin perform less effectively.
Article
Environmental Sciences
Peipei Song, Chengye Sun, Jun Wang, Shiyun Ai, Shujun Dong, Jie Sun, Shuai Sun
Summary: In this study, a combined electrooxidation and electrocoagulation (EO-EC) reactor was used to treat Cu-EDTA wastewater, with parameters like current density, electrolyte, and NaCl concentration found to affect the treatment efficiency. Under specific conditions, high Cu-EDTA removal efficiency was achieved, possibly through the generation of various oxidation and mineralization products. Overall, the EO-EC process proved to be an effective method for treating Cu-EDTA contaminated wastewater.
Article
Environmental Sciences
Shi Zhou, Xue Ni, Houlang Zhou, Xiangmin Meng, Huimin Sun, Jun Wang, Xianqiang Yin
Summary: The study found that biochar nanoparticles can enhance the mobility of Cd2+, but the transport of biochar is limited in the presence of Cd2+. Additionally, the ability of nZVI-NBC to rerelease Cd2+ is weaker than that of NBC, as oxidized nZVI is more easily retained in the sand column, strengthening the immobilization of Cd2+.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Engineering, Environmental
Lin Liu, Yu-Ping Yang, Gui-Lan Duan, Jun Wang, Xian-Jin Tang, Yong-Guan Zhu
Summary: The addition of citric acid was found to significantly enhance arsenic mobilization in contaminated paddy soils, while also inhibiting further transformation. Moreover, it enriched bacteria associated with arsenic and iron reduction processes.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Baihui Shi, Chao Cheng, Yuanqing Zhang, Zhongkun Du, Lusheng Zhu, Jun Wang, Jinhua Wang, Bing Li
Summary: The emerging contaminants PHCZs have been detected globally in soils and sediments, showing dioxin-like toxicity. This study investigated the degradation of 3,6-DCCZ in soil, finding transformation to 3-CCZ and changes in soil bacterial community structure due to exposure. The results suggest that exposure to 3,6-DCCZ affects soil microbial processes related to nitrification, nitrogen fixation, denitrification, carbon dioxide fixation, and hydrocarbon degradation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Nan Jiang, Peipei Song, Xianxu Li, Lusheng Zhu, Jinhua Wang, Xianqiang Yin, Jun Wang
Summary: The study found that low concentrations of DBP can cause oxidative damage and genotoxicity in the zebrafish brain, demonstrating clear toxic effects. Assessment of DBP's toxic effects on zebrafish brain can be effectively evaluated by measuring biomarkers such as SOD and CAT activities.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Environmental
Lingxi Han, Tong Liu, Kuan Fang, Xianxu Li, Xiangwei You, Yiqiang Li, Xiuguo Wang, Jun Wang
Summary: This study investigated indigenous functional microbial communities associated with the degradation of chloroacetamide herbicides in soil. Biodegradation was found to be the main process for the degradation of ACE, S-MET and their enantiomers, with specific bacterial genera like Amycolatopsis and Saccharomonospora playing key roles in the preferential degradation of the S-enantiomers.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Zikun Dong, Xiaojing Yan, Jinhua Wang, Lusheng Zhu, Jun Wang, Chenyu Li, Wenjie Zhang, Shengfang Wen, Young Mo Kim
Summary: This study isolated a bacterium Bacillus cereus H38 that can use sulfamethazine (SMZ) as its only carbon source, and investigated the effects of environmental factors on the biodegradation of SMZ. The results showed that the maximum degradation rate of SMZ was achieved under the conditions of an initial SMZ concentration of 5 mg/L, pH of 7.0, temperature of 25 degrees C and inoculation amount of 5%. The study also identified possible intermediates and proposed two degradation pathways.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Ruolin Wu, Tongtong Zhou, Jun Wang, Jinhua Wang, Zhongkun Du, Bing Li, Albert Juhasz, Lusheng Zhu
Summary: This study found that trifloxystrobin exposure induced oxidative stress and DNA damage in earthworms, with greater subchronic toxicity in brown soil compared to artificial soil. Toxicological experiments of trifloxystrobin in artificial soil may underestimate its toxicity to Eisenia fetida.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Zhongkang Yang, Youai Zhang, Zhouqing Xie, Jun Wang, Zhaolei Li, Yanqiang Li, Jinlong Du, Liguang Sun
Summary: The study revealed that only Pb, P, CaO, TOC, and CIA showed an increasing trend in the upper 7 cm section of the sediment cores, while most of the elements' concentrations decreased towards the surface. The rapid increase of TOC contents is likely related to climate warming, promoting vegetation prosperity and leading to more input of organic matter into lakes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Physical
Lingsong Wang, Huanshun Yin, Suo Wang, Jun Wang, Shiyun Ai
Summary: Bi/BiOCl/Bi2O2CO3 heterojunction with enhanced photocatalytic activity was prepared via one-step hydrothermal process under the synergistic catalysis of Ni2+, showing promising application potential in environmental remediation.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2022)
Article
Engineering, Environmental
Peipei Song, Nan Jiang, Kaiqu Zhang, Xianxu Li, Na Li, Youai Zhang, Qian Wang, Jun Wang
Summary: This study used zebrafish as a model to investigate the ecotoxicological effects of DBP, revealing oxidative stress, lipid peroxidation, and DNA damage in zebrafish liver. The results suggest that DBP poses significant potential risks to aquatic organisms.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Youai Zhang, Zhongkang Yang, Xianxu Li, Peipei Song, Jun Wang
Summary: This study revealed the toxic mechanism of DINP on earthworms, including its effects on ROS levels, MDA content, and antioxidant enzyme activity, as well as its impact on the gut bacteria of earthworms and surrounding soil bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Xianxu Li, Na Li, Can Wang, Anyu Wang, Wenjia Kong, Peipei Song, Jun Wang
Summary: This study investigated the occurrence of phthalic acid esters (PAEs) in protected agriculture soils and assessed their potential health risks to humans. The results showed that DEHP and DBP were the most abundant PAEs found in the soils, while DMP and DEP were not detected. Health risk assessment revealed that children have higher health risks compared to adults.
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2022)
Article
Environmental Sciences
Xiaohui Ji, Xiangfeng Yao, Xianxu Li, Lusheng Zhu, Jinhua Wang, Jun Wang
Summary: The aim of this study was to investigate the effects of environmental concentrations of atrazine on the soil microbial community. The results showed that the number of culturable bacteria, fungi, and actinomycetes decreased significantly with the increase of atrazine concentration, showing a certain dose-effect relationship. Overall, this study demonstrates that atrazine has a certain influence on soil microorganisms and provides an objective assessment of the ecological risk of atrazine to soil microorganisms.
WATER AIR AND SOIL POLLUTION
(2023)
