4.7 Article

Dibutyl phthalate induced oxidative stress and genotoxicity on adult zebrafish (Danio rerio) brain

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 424, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.jhazmat.2021.127749

关键词

Dibutyl phthalate; Zebrafish; Oxidative stress; DNA damage; Gene expression

资金

  1. National Natural Science Foundation of China [U2106212, 42177024, 42177069, 41807125, U1906225]
  2. Key Research and Development Program of Shan-dong Province [2019GSF109026]
  3. National Key Research and Development Project of China [2018YFC1800605]
  4. Special Funds of Taishan Scholar of Shan-dong Province, China

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The study found that low concentrations of DBP can cause oxidative damage and genotoxicity in the zebrafish brain, demonstrating clear toxic effects. Assessment of DBP's toxic effects on zebrafish brain can be effectively evaluated by measuring biomarkers such as SOD and CAT activities.
Dibutyl phthalate (DBP) is one of the most widely used plasticizers with a high concentration in the water. Although the toxicity of DBP on aquatic organisms has become a significant concern in recent years, the effects of DBP on zebrafish (Danio rerio) brain is poorly understood. This study investigated the toxic effects of DBP exposure for 7, 14, 21 and 28 days on zebrafish brain. The results showed that DBP significantly stimulated SOD and CAT activities, increasing MDA and 8-OHdG contents. On the 28th day, the AChE inhibition rates in 0.08, 0.4, 2 mg center dot L-1 treatment were 13.4%, 11.9%, 14.7%. The trend of Cu/Zn-sod gene variation was consistent with SOD activity, showing inhibition-activation-inhibition. The expression of apoptotic genes (caspase-3, p53) showed inhibition-activation-inhibition. The integrated biomarker response (IBR) results showed that the IBR values were 4.37, 7.18 and 9.63 in 0.08, 0.4 and 2 mg center dot L-1 group on the 28th day, presenting a dose-response relationship. These findings confirmed that low concentration of DBP induced oxidative damage and genotoxicity in zebrafish brain, which provided an effective toxicological basis for phthalate pollution. Based on above studies, it is of great significance for assessing the harmful effects of DBP with low concentration on aquatic organisms.

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