Article
Multidisciplinary Sciences
Delphine Lissa, Nobuyuki Takahashi, Parth Desai, Irena Manukyan, Christopher W. Schultz, Vinodh Rajapakse, Moises J. Velez, Deborah Mulford, Nitin Roper, Samantha Nichols, Rasa Vilimas, Linda Sciuto, Yuanbin Chen, Udayan Guha, Arun Rajan, Devon Atkinson, Rajaa El Meskini, Zoe Weaver Ohler, Anish Thomas
Summary: This study explores the clinical significance and biological characteristics of neuroendocrine subtypes in small cell lung cancer, as well as the complex variability within and between tumors. NE tumors are more likely to respond to certain treatments but show resistance to chemotherapy, while non-NE tumors may benefit more from immunotherapy.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Joaquin Letelier, Lorena Buono, Maria Almuedo-Castillo, Jingjing Zang, Constanza Mounieres, Sergio Gonzalez-Diaz, Rocio Polvillo, Estefania Sanabria-Reinoso, Jorge Corbacho, Ana Sousa-Ortega, Ruth Diez del Corral, Stephan C. F. Neuhauss, Juan R. Martinez-Morales
Summary: Genetic studies have shown that Vsx genes play a dual role in retina development, being involved in progenitor specification and determination of bipolar cell fate. However, the extent to which Vsx functions are conserved across vertebrates is still unclear. In this study, Vsx1 and Vsx2 were knocked out in zebrafish embryos using CRISPR/Cas9, resulting in severe visual impairment and depletion of bipolar cells. Surprisingly, the neural retina was properly specified and maintained in mutant embryos. The findings suggest that genetic redundancy is an important mechanism for maintaining the integrity of the retinal specification network.
Article
Multidisciplinary Sciences
Guadalupe Gutierrez, Peng Sun, Yingying Han, Xing Dai
Summary: Breast cancer is a heterogeneous disease, and understanding the heterogeneity within the normal mammary basal epithelial cells is crucial. In this study, we used single-cell RNA-sequencing and RNA in-situ hybridization to characterize the heterogeneity of mammary basal cells. We identified four major transcriptional states within the basal cells, one of which represents a dynamic state during pubertal mammary morphogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Deepak A. Kaji, Angela M. Montero, Roosheel Patel, Alice H. Huang
Summary: This study identifies transcriptional regulators involved in the induction and differentiation of dense connective tissues. By generating tendon and fibrocartilage cells from mouse embryonic stem cells and utilizing scRNA-seq, the authors reveal molecular mechanisms underlying cell fate switch between these lineages. Additionally, the research demonstrates successful generation of 3D engineered tissues using these differentiation protocols for modeling development and disease.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Edroaldo Lummertz da Rocha, Caroline Kubaczka, Wade W. Sugden, Mohamad Ali Najia, Ran Jing, Arianna Markel, Zachary C. LeBlanc, Rafael dos Santos Peixoto, Marcelo Falchetti, James J. Collins, Trista E. North, George Q. Daley
Summary: The study presents an algorithm called CellComm that analyzes cell-cell communication, predicts signaling and regulatory networks, and identifies regulators of hematopoietic development. Using zebrafish, mouse, and human models, the study confirms the accuracy of CellComm in identifying key regulators of hematopoiesis.
NATURE CELL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Zizhong Hu, Xiying Mao, Mingkang Chen, Xinjing Wu, Tianye Zhu, Yu Liu, Zhengyu Zhang, Wen Fan, Ping Xie, Songtao Yuan, Qinghuai Liu
Summary: This study utilized single-cell RNA sequencing to characterize proliferative diabetic retinopathy (PDR) vitreous fibrovascular membranes (FVMs). They identified eight cellular compositions, with microglia as the major cell population. They discovered a profibrotic subpopulation of microglia that was uniquely differentiated from retina-resident microglia and expanded in the PDR setting. Ligand-receptor interactions between the profibrotic microglia and cytokines upregulated in PDR vitreous implicated the involvement of several pathways in the microglial activation within the PDR microenvironment.
Article
Biology
Megan M. Colonnetta, Lauren R. Lym, Lillian Wilkins, Gretchen Kappes, Elias A. Castro, Pearl Ryder, Paul Schedl, Dorothy A. Lerit, Girish Deshpande
Summary: Transcriptional quiescence is an evolutionarily conserved trait that distinguishes the embryonic primordial germ cells (PGCs) from somatic cells, with Sex-lethal (Sxl) being a biologically relevant transcriptional target of Gcl. The mutual antagonism between Gcl and Torso ensures controlled release of germ-plasm, highlighting the importance of transcriptional silencing mediated by Gcl. Embryos expressing a degradation-resistant form of Torso in the germline show aberrant spreading of pole plasm RNAs, similar to gcl mutants, indicating a role in the distinction between germline and soma.
Article
Oncology
Nicholas Owen, Maria Toms, Yuan Tian, Lyes Toualbi, Rose Richardson, Rodrigo Young, Dhani Tracey-White, Pawan Dhami, Stephan Beck, Mariya Moosajee
Summary: The oko meduzy (m289/m289) (crb2a(-/-)) zebrafish and CRB1 patient-derived retinal organoids showed delayed retinal cell specification, dysfunction in cell cycle modulation, and epigenetic transcriptional control. Differential DNA methylation analysis revealed novel hypermethylated pathways involving biological adhesion, Hippo, and transforming growth factor beta (TGF-beta) signalling. Integration of gene expression and DNA methylation using functional epigenetic modules revealed six key modules involving cell cycle control and disturbance of TGF-beta, bone morphogenetic protein (BMP), Hippo, and SMAD protein signal transduction pathways.
JOURNAL OF PATHOLOGY
(2023)
Article
Neurosciences
Jens Schuster, Joakim Klar, Ayda Khalfallah, Loora Laan, Jan Hoeber, Ambrin Fatima, Velin Marita Sequeira, Zhe Jin, Sergiy V. Korol, Mikael Huss, Ann Nordgren, Britt Marie Anderlid, Caroline Gallant, Bryndis Birnir, Niklas Dahl
Summary: This study investigates the dysregulated gene network and altered electrophysiological properties in ZEB2 haploinsufficient GABAergic development, providing insights into the neuropathogenesis and seizures in MWS.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Melissa K. Jones, Devansh Agarwal, Kevin W. Mazo, Manan Chopra, Shawna L. Jurlina, Nicholas Dash, Qianlan Xu, Anna R. Ogata, Melissa Chow, Alex D. Hill, Netra K. Kambli, Guorong Xu, Roman Sasik, Amanda Birmingham, Kathleen M. Fisch, Robert N. Weinreb, Ray A. Enke, Dorota Skowronska-Krawczyk, Karl J. Wahlin
Summary: Retinogenesis involves the specification of retinal cell types during early vertebrate development. Pluripotent stem cell (PSC) derived retinal organoids offer an experimentally accessible solution for investigating the developing human retina, providing valuable insights into the molecular nature of eye development.
Article
Biochemistry & Molecular Biology
Francesca Gabanella, Andrea Colizza, Maria Chiara Mottola, Silvia Francati, Giovanna Blacona, Carla Petrella, Christian Barbato, Antonio Greco, Massimo Ralli, Marco Fiore, Nicoletta Corbi, Giampiero Ferraguti, Alessandro Corsi, Antonio Minni, Marco de Vincentiis, Claudio Passananti, Maria Grazia Di Certo
Summary: Head and neck squamous cell carcinoma (HNSCC) originates from the mucosal epithelium and laryngeal squamous cell carcinoma (LSCC) is a subtype of HNSCC. This study focuses on the role of the Survival Motor Neuron (SMN) protein in LSCC and explores its impact on cancer-related behaviors. The study also reveals a physical interaction between SMN and the epidermal growth factor receptor (EGFR) in LSCC. Overall, this study highlights SMN as a potential therapeutic target for LSCC and other subtypes of HNSCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tahani W. Baakdhah, Brenda Coles, Derek van der Kooy
Summary: Research shows that retinal stem cells have the ability to generate various retinal cell types, with central pigmented spheres exhibiting high proliferative capacity and the capability to produce a large number of retinal pigment epithelial cells. In contrast, the other sphere types primarily generate neural cells.
Article
Cell & Tissue Engineering
Xiaoman Wang, Veeramohan Veerapandian, Xinyan Yang, Ke Song, Xiaoheng Xu, Manman Cui, Weiyan Yuan, Yaping Huang, Xinyu Xia, Zhaokai Yao, Cong Wan, Fang Luo, Xiuling Song, Xiaoru Wang, Yi Zheng, Andrew Paul Hutchins, Ralf Jauch, Meiyan Liang, Chenhong Wang, Zhaoting Liu, Gang Chang, Xiao-Yang Zhao
Summary: The study identified the crucial roles of SOX15 in maintaining human primordial germ cell-like cells through suppressing somatic gene expression and sustaining pluripotency. Additionally, ETV5 was also found to be essential for the maintenance of primordial germ cell-like cells.
Review
Biochemistry & Molecular Biology
Wooseok Seo, Chandsultana Jerin, Hiroyoshi Nishikawa
Summary: Exhausted CD8(+) T cells may be deliberately generated and maintained to provide mild immune responses against chronic infection or cancer, which can be safer over the long term compared to strong immune responses. A specific population of exhausted CD8(+) T cells that behaves like self-renewing stem cells has been identified, suggesting they can be considered progenitors of exhausted CD8(+) T cells. Studies have revealed the regulatory network governing the generation and propagation of exhausted CD8(+) T cells, shedding light on the developmental stages that lead to this subtle but effective immune response.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Geriatrics & Gerontology
Xianzhong Zhang, Guanglou Zhu, Fengmin Zhang, Dingye Yu, Xuyang Jia, Bingwei Ma, Weizhe Chen, Xinyu Cai, Lingzhou Mao, Chengle Zhuang, Zhen Yu
Summary: This study identified an immune-related transcriptional regulatory network in sarcopenia using RNA-seq data analysis. The PAX5-SERPIAN5-PI3K/Akt axis was suggested as a potential mechanism for sarcopenia, with B cells playing a vital role in this signal transduction. Trichostatin A was identified as a potential medicine for sarcopenia.
Article
Biology
Pauline Cabochette, Guillermo Vega-Lopez, Juliette Bitard, Karine Parain, Romain Chemouny, Christel Masson, Caroline Borday, Marie Hedderich, Kristine A. Henningfeld, Morgane Locker, Odile Bronchain, Muriel Perron
Review
Anatomy & Morphology
Annaig Hamon, Jerome E. Roger, Xian-Jie Yang, Muriel Perron
DEVELOPMENTAL DYNAMICS
(2016)
Article
Multidisciplinary Sciences
Thorsten Pfirrmann, Enrico Jandt, Swantje Ranft, Ashwin Lokapally, Herbert Neuhaus, Muriel Perron, Thomas Hollemann
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Neurosciences
Rahul Langhe, Albert Chesneau, Gabriele Colozza, Magdalena Hidalgo, Divya Ail, Morgane Locker, Muriel Perron
Article
Cell Biology
Cataldo Schietroma, Karine Parain, Amrit Estivalet, Asadollah Aghaie, Jacques Boutet de Monvel, Serge Picaud, Jose-Alain Sahel, Muriel Perron, Aziz El-Amraoui, Christine Petit
JOURNAL OF CELL BIOLOGY
(2017)
Article
Developmental Biology
Nathalie Bessodes, Karine Parain, Odile Bronchain, Eric J. Bellefroid, Muriel Perron
NEURAL DEVELOPMENT
(2017)
Article
Multidisciplinary Sciences
Caroline Borday, Karine Parain, Hong Thi Tran, Kris Vleminckx, Muriel Perron, Anne H. Monsoro-Burq
Article
Cell Biology
Annaig Hamon, Diana Garcia-Garcia, Divya Ail, Juliette Bitard, Albert Chesneau, Deniz Dalkara, Morgane Locker, Jerome E. Roger, Muriel Perron
Article
Cell Biology
Christel Masson, Diana Garcia-Garcia, Juliette Bitard, Elodie-Kim Grellier, Jerome E. Roger, Muriel Perron
CELL DEATH & DISEASE
(2020)
Article
Neurosciences
Elena Kisseleff, Robin J. Vigouroux, Catherine Hottin, Sophie Lourdel, Leah Thomas, Parth Shah, Alain Chedotal, Muriel Perron, Anand Swaroop, Jerome E. Roger
Summary: The study reveals a unique role of GSK3 in controlling the production of ganglion cells in the inner nuclear layer, corresponding to dRGCs, a rare and poorly characterized retinal cell type. In Gsk3 mutant mice, an excess of dRGCs leads to more axons projecting into the non-image-forming visual circuit, and at least a subset of dRGCs are direction-selective RGCs.
Article
Biology
Rodrigo Melendez Garcia, Olivier Haccard, Albert Chesneau, Hemalatha Narassimprakash, Jerome Roger, Muriel Perron, Kathrin Marheineke, Odile Bronchain
Summary: In this study, researchers discovered that Yap, a downstream effector of the Hippo signalling pathway, plays a crucial role in regulating DNA replication dynamics. Yap is recruited to chromatin at the start of DNA replication and binds to Rif1, a major regulator of the DNA replication timing program. Depletion of Yap or Rif1 accelerates DNA replication by increasing the number of activated replication origins. The findings also suggest that Yap or Rif1 depletion leads to shorter S-phase and faster cell divisions.
Review
Cell Biology
Catherine Hottin, Muriel Perron, Jerome E. Roger
Summary: Glycogen synthase kinase 3 (GSK3) plays a crucial role in the nervous system and inhibiting it may be a promising therapeutic approach for neurodegenerative diseases. This review summarizes the literature on the role of GSK3 in retinal degeneration and highlights its potential effects on glucose homeostasis, oxidative stress, inflammation, neovascularization, and cell-based therapy.
Article
Ophthalmology
Annaig Hamon, Christel Masson, Juliette Bitard, Linn Gieser, Jerome E. Roger, Muriel Perron
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2017)
Article
Cell Biology
Divya Ail, Muriel Perron
CURRENT PATHOBIOLOGY REPORTS
(2017)