Article
Biochemistry & Molecular Biology
Martin Bablok, Morris Gellisch, Beate Brand-Saberi, Gabriela Morosan-Puopolo
Summary: Understanding the complex processes of fetal wound healing and skin regeneration can improve fetal surgery, but local glucocorticoid administration may decelerate the healing of skin incisional wounds, resulting in morphological changes.
Article
Biochemistry & Molecular Biology
Silvia Concetta Colucci, Cinzia Buccoliero, Lorenzo Sanesi, Mariella Errede, Graziana Colaianni, Tiziana Annese, Mohd Parvez Khan, Roberta Zerlotin, Manuela Dicarlo, Ernestina Schipani, Kenneth M. Kozloff, Maria Grano
Summary: The study found that irisin promotes bone formation and accelerates the fracture healing process, suggesting its potential use as a novel pharmacologic modulator.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Shreyas Bhave, Lija Swain, Xiaoying Qiao, Gregory Martin, Tejasvi Aryaputra, Kay Everett, Navin K. Kapur
Summary: This study reveals that reduced ALK1 activity leads to decreased survival and increased cardiac fibrosis after myocardial infarction (MI). The data also suggest that ALK1 is involved in the regulation of growth factors, fibrotic indicators, and other signaling pathways after MI. These findings suggest that ALK1 could be a potential therapeutic target to improve survival after MI.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Mediha Becirovic-Agic, Upendra Chalise, Mira Jung, Jocelyn R. Rodriguez-Paar, Shelby R. Konfrst, Elizabeth R. Flynn, Jeffrey D. Salomon, Michael E. Hall, Merry L. Lindsey
Summary: The speed of skin wound healing predicts the healing response of the heart after myocardial infarction. Two plasma proteins, EAF1 and A2M, at day 3 of myocardial infarction can predict the risk of death in 66% of cases. ApoD negatively regulates skin and cardiac wound healing by promoting inflammation. Skin acts as a reflection of the heart, with common pathways linking wound healing across different organs.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Wenjie Zuo, Renhua Sun, Zhenjun Ji, Genshan Ma
Summary: Macrophages play a crucial role in the inflammatory repair process following myocardial infarction. The traditional classification of macrophages as either pro-inflammatory or pro-reparative falls short of precision medicine standards. Advances in single-cell sequencing technology have opened new avenues for studying macrophage heterogeneity and plasticity.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Virology
Arianna Baronti, Francesco Gentile, Alice Chiara Manetti, Andrea Scatena, Silvia Pellegrini, Angela Pucci, Maria Franzini, Vincenzo Castiglione, Aniello Maiese, Alberto Giannoni, Mauro Pistello, Michele Emdin, Giovanni Donato Aquaro, Marco Di Paolo
Summary: This study presents five cases of sudden death secondary to myocardial infarction (MI) closely related to COVID-19 vaccination. Pathological analysis and genetic testing revealed that the patients carried pro-thrombotic mutations, suggesting a possible link between COVID-19 vaccination, pro-thrombotic genotypes, and acute cardiovascular events that requires further investigation.
Article
Cell Biology
Tina B. Marvasti, Faisal J. Alibhai, Lukasz Wlodarek, Anne Fu, Shu-Hong Li, Jun Wu, Richard D. Weisel, Robert J. Cusimano, Maral Ouzounian, Terrence Yau, Ren-Ke Li
Summary: This study investigated the impact of aging on human bone marrow cell responses post-myocardial infarction. The findings revealed that younger patient cells had greater repopulation capacity and lymphoid cell production, while the age of CD34(+) cells influenced donor and host cellular responses following MI.
Review
Medicine, Research & Experimental
Yu-Yan Xiong, Zhao-Ting Gong, Rui-Jie Tang, Yue-Jin Yang
Summary: AMI is a leading cause of mortality globally, with inflammatory response playing a pivotal role in myocardial necrosis and ventricular remodeling. Exosomes, containing biologically active substances, have emerged as key players in immune regulation post AMI, alongside a variety of immune cells working together for tissue cleanup and repair. Research into exosomes from both endogenous immune cells and exogenous stem cells has shown promising therapeutic effects in immunomodulation for myocardial repair post AMI.
Article
Cardiac & Cardiovascular Systems
Akihiko Kubota, Akira Suto, Kensuke Suga, Arifumi Iwata, Shigeru Tanaka, Kotaro Suzuki, Yoshio Kobayashi, Hiroshi Nakajima
Summary: The study reveals that IL-21 induces apoptosis of Ly6Clow macrophages, reducing survival rates after MI and delaying wound healing. Meanwhile, IL-21 secreted by CD4+ T cells plays a crucial role in this process.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Di Wang, Yaping Zhang, Tianbao Ye, Runlei Zhang, Lili Zhang, Dongmei Shi, Taixi Li, Guofang Xia, Kaifan Niu, Zhe Zhao, Yu Chen, Weijun Pan, Liang Liu, Xian Jin, Chengxing Shen
Summary: Cardiac fibroblasts play a crucial role in scar formation and cardiac repair after myocardial infarction (MI). The extracellular matrix protein CTHRC1 is involved in vascular remodeling, bone formation, and tumor progression. However, the role and mechanism of CTHRC1 in post-MI wound repair are not fully understood.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Endocrinology & Metabolism
Arthi Thirumalai, Bradley D. Anawalt
Summary: This narrative review discusses the controversy surrounding the cardiovascular effects of endogenous and exogenous testosterone in men. The evidence suggests that physiological dosages of testosterone have minimal to no effect on cardiovascular outcomes, but there is insufficient evidence to support testosterone therapy for high-risk patients. Clinicians should avoid prescribing supraphysiological testosterone therapy to hypogonadal men.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2022)
Article
Multidisciplinary Sciences
Donghoon Han, Si-Hyuck Kang, Chang-Hwan Yoon, Tae-Jin Youn, In-Ho Chae
Summary: This study investigated the efficacy of intracoronary administration of EDTA as an adjunctive treatment to reduce ischemia-reperfusion (IR) injury in a swine model. The results showed that EDTA significantly reduced infarct size, mitochondrial damage, and post-infarct remodeling. Further clinical studies are warranted to evaluate the efficacy of EDTA in patients with ST-segment elevation myocardial infarction.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Micaela A. Reeves, Joshua M. Royal, David A. Morris, Jessica M. Jurkiewicz, Nobuyuki Matoba, Krystal T. Hamorsky
Summary: EPT, a recombinant variant of cholera toxin B subunit, shows promise for ulcerative colitis treatment. A solid oral formulation of EPT has been developed to prevent degradation in gastric acid, demonstrating therapeutic efficacy in mouse colitis models without the need for gastric acid neutralization pretreatment.
Article
Engineering, Biomedical
Xuechong Hong, Allen Chilun Luo, Ilias Doulamis, Nicholas Oh, Gwang-Bum Im, Chun-Yen Lin, Pedro J. del Nido, Ruei-Zeng Lin, Juan M. Melero-Martin
Summary: This study developed a cell delivery method using a photocrosslinkable GelMA hydrogel, which significantly improved cell retention and promoted cardiac healing in myocardial infarction. The engrafted vascular cells regulated the activation state of neutrophils, creating a pro-regenerative microenvironment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Jinwoo Myung, Jin-Ho Beom, Ju-Hee Kim, Ji-Sun Woo, Incheol Park, Sung-Phil Chung, Yong-Eun Chung, Je-Sung You
Summary: This study found that the administration of recombinant klotho (rKL) protein can attenuate the inflammation and cell death caused by myocardial ischemia and reperfusion injury (I/R) by inhibiting the extracellular release of HMGB1. This provides a theoretical basis for the clinical adjunctive treatment of acute myocardial infarction.
Article
Toxicology
Juliane Wunder, Daniela Pemp, Alexander Cecil, Maryam Mahdiani, Rene Hauptstein, Katja Schmalbach, Leo N. Geppert, Katja Ickstadt, Harald L. Esch, Thomas Dandekar, Leane Lehmann
Summary: Breast cancer etiology involves proliferation and DNA damage induced by estrogens. Recent studies show that breast cancer risk factors such as body mass index, smoking, and intake of estrogen-active drugs can influence intratissue estrogen levels.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Cell Biology
Esther Klaile, Juan P. Prada Salcedo, Tilman E. Klassert, Matthias Besemer, Anne-Katrin Bothe, Adrian Durotin, Mario M. Mueller, Verena Schmitt, Christian H. Luther, Marcus Dittrich, Bernhard B. Singer, Thomas Dandekar, Hortense Slevogt
Summary: The study demonstrates that ligation of CEACAM receptors has differential effects on the immune functions of human neutrophils in response to C. albicans, altering apoptosis, cytokine secretion, and other responses.
CELLULAR IMMUNOLOGY
(2022)
Article
Immunology
Juan Gamboa Vargas, Jennifer Wagner, Haroon Shaikh, Isabell Lang, Juliane Medler, Mohamed Anany, Tim Steinfatt, Josefina Pena Mosca, Stephanie Haack, Julia Dahlhoff, Maike Buettner-Herold, Carolin Graf, Estibaliz Arellano Viera, Hermann Einsele, Harald Wajant, Andreas Beilhack
Summary: In this study, a new fusion protein called NewSTAR2 was developed with improved serum retention and superior in vivo activity, showing powerful protective effects against acute GvHD.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Moustafa Fathy, Rania Abdel-latif, Yasmeen M. Abdelgwad, Othman Ali Othman, Abdel-Razik H. Abdel-Razik, Thomas Dandekar, Eman M. Othman
Summary: The study showed that eugenol has a potential protective effect against CCl4-induced chronic kidney damage in an experimental model, mainly through its antioxidant, anti-inflammatory, and anti-fibrotic activities.
Article
Multidisciplinary Sciences
Muharrem Aydinli, Chunguang Liang, Thomas Dandekar
Summary: AIModules is an integrated solution for motif analysis that can identify nucleic acid motifs and combinations, providing various biological applications. It is able to quickly discover TFBS and shared modules, outperforming other available software in terms of speed and module discovery.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Daniela Siegmund, Jennifer Wagner, Harald Wajant
Summary: TRAF2 is a protein that interacts with TNFR2 and other TNFRSF receptors, and is involved in the activation of multiple signaling pathways. It plays a role in NFkB pathway, MAP kinase cascades, ER stress signaling, autophagy regulation, and cell death control. TRAF2 can function as a scaffold and E3 ligase, and is implicated in the oncogenesis and tumor suppression of various cancers. Targeting TRAF2 shows potential for therapeutic purposes.
Article
Biochemistry & Molecular Biology
Edita Sarukhanyan, Thomas Dandekar
Summary: Microtubules are essential for maintaining cell shape and Tau protein plays a role in regulating their plasticity. Abnormal phosphorylation of Tau proteins leads to the formation of neurofibrillary tangles, which are linked to Alzheimer's disease. In this study, a potential drug candidate, compound 23, was designed using computer simulations to target the phosphorylated Tau-microtubule complexes. The results showed that compound 23 can bind to and stabilize phosphorylated Tau proteins on the surface of microtubules, preventing their aggregation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Elena Bencurova, Aman Akash, Renwick C. J. Dobson, Thomas Dandekar
Summary: In this article, the fundamental aspects of DNA storage and recent advances in this field are discussed, with a focus on natural processes and solutions that can be utilized. New methods for efficient DNA and nucleotide storage inspired by nature are highlighted. Within a few years, DNA-based information storage may become a complement to current electronic data storage systems. Various aspects, such as rapid and directed access, regulatory signals and modulation, and integrated high-density storage and processing modules, are examined. DNA storage has practical applications in biotechnology and human genetics. The potential of DNA storage in synthetic biology and its advantages in direct storage operations are also explored. The use of biology concepts from chromosomal storage, nucleic acid processing, and polymer material sciences will lead to new applications gradually replacing classical information storage methods over time (decades).
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Chao Han, Pengxuan Ren, Medina Mamtimin, Linus Kruk, Edita Sarukhanyan, Chenyu Li, Hans-Joachim Anders, Thomas Dandekar, Irena Krueger, Margitta Elvers, Silvia Goebel, Kristin Adler, Goetz Muench, Thomas Gudermann, Attila Braun, Elmina Mammadova-Bach
Summary: This study analyzed the collagen- and CRP-binding surfaces of GPVI monomers and dimers using computational modeling and bioinformatics, and compared them with other mammalian GPVI isoforms. Experimental results show that the EA-20 antibody inhibits platelet adhesion and aggregation in response to collagen and CRP, but not to fibrin, and prevents thrombus formation in whole blood. Therefore, the blockade of this minimal collagen-binding epitope of GPVI with the EA-20 antibody could represent a new anti-thrombotic approach.
Review
Biochemistry & Molecular Biology
Constanze Schanbacher, Heike M. M. Hermanns, Kristina Lorenz, Harald Wajant, Isabell Lang
Summary: This review provides a brief summary of the biology of CTRPs and focuses on the structure, receptors, and major signaling pathways of CTRPs. Studies on CTRP knockout mice and CTRP transgenic mice support the anti-inflammatory and insulin-sensitizing effects of CTRPs in autoimmune diseases, obesity, atherosclerosis, and cardiac dysfunction. CTRPs can form different homo- and heterotypic complexes with various activities, and the activation of their receptors and downstream signaling pathways are still largely unknown. The limited knowledge of CTRP redundancy and signal transduction hampers the translational usage of CTRPs as therapeutic targets.
Article
Biochemistry & Molecular Biology
Kunkun Li, Juan Prada, Thomas Dandekar, Daniel S. C. Damineli, Kai R. R. Konrad
Summary: The polar growth and guidance of pollen tubes are regulated by chemical and electrical signaling cues. The functional relationship between membrane voltage (V-m), Ca2+, and H+ dynamics in pollen tubes is largely unclear. In this study, live-cell imaging and cross-correlation analysis revealed distinct relationships between these signals along pollen tubes, with Ca2+ and H+ correlating with V-m dynamics at the tip, while only H+ correlated with V-m at the shank. These findings suggest spatially confined chemo-electric coupling supports the propagation of voltage and pH waves along non-growing pollen tubes, preceded by short-lasting but high amplitude tip Ca2+ spikes.
Article
Biochemistry & Molecular Biology
Aylin Caliskan, Deniz Caliskan, Lauritz Rasbach, Weimeng Yu, Thomas Dandekar, Tim Breitenbach
Summary: Machine learning techniques are widely used to analyze single-cell expression data, impacting various fields such as cell annotation, clustering, and signature identification. This framework evaluates gene selection sets based on their ability to effectively separate defined phenotypes or cell groups. It overcomes the limitation of identifying a small gene set with high information content for phenotype separation, and provides corresponding code scripts. The selected subset of genes facilitates human interpretability of phenotype differences, and can potentially explain the causal relationships between genes and phenotypes.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Rana Salihoglu, Mugdha Srivastava, Chunguang Liang, Klaus Schilling, Aladar Szalay, Elena Bencurova, Thomas Dandekar
Summary: PRO-Simat is a simulation tool that analyzes protein interaction networks, their dynamic changes, and pathway engineering. It provides GO enrichment, KEGG pathway analyses, and network visualization using a database of over 8 million protein-protein interactions across 32 organisms. The tool also allows for dynamical network simulation using the Jimena framework, offering in-depth analysis of protein interactions. It has demonstrated applications in understanding differentiation pathways, oncolytic virus development, and optogenetic control of nucleotide processing protein networks. PRO-Simat can be accessed at https://prosimat.heinzelab.de/ as a web-based query server.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Aylin Caliskan, Seema Dangwal, Thomas Dandekar
Summary: In the rapidly evolving field of biomedical research, the emergence of big data has provided researchers with opportunities to explore biological complexities. However, to ensure the reliability and reproducibility of data-driven findings, it is crucial to have detailed and accurate metadata.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
