Article
Microbiology
Marina Valente Navarro, Yasmin Nascimento de Barros, Wilson Dias Segura, Alison Felipe Alencar Chaves, Grasielle Pereira Jannuzzi, Karen Spadari Ferreira, Patricia Xander, Wagner Luiz Batista
Summary: The study revealed the crucial role of PbDrk1 in cell wall modulation of P. brasiliensis, affecting fungal resistance to different cell wall-disturbing agents and influencing cell wall morphogenesis by regulating various genes associated with cell wall modulation. It also showed an increased phagocytic index and proinflammatory cytokine levels in response to PbDrk1, suggesting its significance as a potential target for antifungal molecular activity.
Article
Biochemistry & Molecular Biology
Lais Alonso, Olivia Basso Rocha, Marcos Antonio Batista de Carvalho Junior, Celia Maria de Almeida Soares, Maristela Pereira, Antonio Alonso
Summary: Electron paramagnetic resonance spectroscopy was used to investigate the interactions of amphotericin B, miltefosine, and nerolidol with the plasma membrane of Paracoccidioides brasiliensis. The results showed that these compounds have different affinities for the membrane, with amphotericin B having the highest affinity. Additionally, the study found that the anti-proliferative effects of these compounds are associated with alterations in the cell membrane.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Relber Aguiar Goncales, Rafael Ricci-Azevedo, Vanessa C. S. Vieira, Fabricio F. Fernandes, Sandra M. de O. Thomaz, Agostinho Carvalho, Patricia E. Vendruscolo, Cristina Cunha, Maria Cristina Roque-Barreira, Fernando Rodrigues
Summary: The study demonstrated that overexpression of PCN in Paracoccidioides brasiliensis enhances cell separation and chitin processing, leading to increased virulence and exacerbation of the disease. This suggests that PCN modulates cell wall architecture through its chitinase activity.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Rafael de Souza Silva, Wilson Dias Segura, Reinaldo Souza Oliveira, Patricia Xander, Wagner Luiz Batista
Summary: Paracoccidioidomycosis (PCM) is a prevalent fungal infection in Latin America, caused by fungi from the Paracoccidioides genus. This study characterized the modulation of acid proteases in Paracoccidioides brasiliensis and found that these proteases are regulated by environmental conditions and fungal thermo-dimorphism. The findings provide new possibilities for studying the role of aspartyl proteases in the host-pathogen relationship and P. brasiliensis biology.
Article
Infectious Diseases
Priscila C. Correa, Fabricio F. Fernandes, Marcelo Costa, Taise N. Landgraf, Ademilson Panunto-Castelo
Summary: This study determined the biochemical properties of an alpha-mannosidase released by Paracoccidioides brasiliensis and found that its gene expression is associated with fungal morphology, stress, and virulence.
Article
Cell Biology
Hongwei Li, Xue Li, Qian Wu, Xing Wang, Zhonghua Qin, Yaguo Wang, Yanbin He, Qi Wu, Li Li, Huaiyong Chen
Summary: In COVID-19 survivors, about 41% reported at least one clinical symptom and 26.5% exhibited lung imaging abnormalities 6 months after discharge. Plasma proteomics and metabolomics analysis showed that COVID-19 survivors differed from healthy controls in terms of extracellular matrix, immune response, and hemostasis pathways. Moreover, COVID-19 survivors also exhibited abnormal lipid metabolism, disordered immune response, and changes in pulmonary fibrosis-related proteins.
CELL DEATH & DISEASE
(2022)
Article
Plant Sciences
Kristina Siposova, Eva Labancova, Danica Kucerova, Karin Kollarova, Zuzana Vivodova
Summary: Auxins can impact plant growth and development, with indole-3-butyric acid (IBA) showing diverse effects on maize root growth under different stress conditions. IBA at specific concentrations can alter the concentration of H2O2, activity of antioxidant enzymes, and mineral nutrient levels in plants. Notably, the application of 10(-9) M IBA had the most positive effects on plants cultivated with Cd, reducing oxidative stress and increasing nutrient uptake.
Article
Hematology
Abraham J. Kandathil, Sarah E. Benner, Evan M. Bloch, Ruchee Shrestha, Olivia Ajayi, Xianming Zhu, Patrizio P. Caturegli, Shmuel Shoham, David Sullivan, Kelly Gebo, Thomas C. Quinn, Arturo Casadevall, Daniel Hanley, Andrew Pekosz, Andrew D. Redd, Ashwin Balagopal, Aaron A. R. Tobian
Summary: By characterizing the DNA and RNA virome of potential CCP donors, it was found that there is no evidence of pathogenic viruses in CCP, despite the presence of non-pathogenic Anelloviridae. These results support the safety of CCP.
Proceedings Paper
Engineering, Biomedical
Jose Alexandre da Silva Junior, R. S. Navarro, A. U. Fernandes, D. Kozusny-Andreani, L. S. Feitosa
Summary: This study evaluated the efficacy of Photodynamic Inactivation (PDI) in eliminating Paracoccidioides brasiliensis, a pathogenic fungus. The results demonstrated that PDI, combined with the photosensitizer curcumin 98%, significantly reduced the presence of the fungus.
XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020
(2022)
Article
Chemistry, Multidisciplinary
Moazameh Adhami Sayad Mahaleh, Mehrnoush Narimisa, Anton Nikiforov, Mikhail Gromov, Yury Gorbanev, Rim Bitar, Rino Morent, Nathalie De Geyter
Summary: The recent energy crisis has highlighted the need to find alternative and sustainable methods for industrial nitrogen fixation. Plasma-based nitrogen fixation is a promising option, especially when plasma interacts with water. In this study, the effect of water presence on nitrogen oxidation in a multi-pin plasma system was experimentally investigated. The results showed that the presence of water increased the energy cost of the process by up to 14 ± 8%.
APPLIED SCIENCES-BASEL
(2023)
Review
Nutrition & Dietetics
Alexandra W. C. Einerhand, Wiola Mi, Alfred Haandrikman, Xiao-Yang Sheng, Philip C. Calder
Summary: Linoleic acid and α-linolenic acid are essential fatty acids crucial for health. Levels of linoleic acid in breast milk and infant formula vary across countries, and this study aims to review the global levels of these fatty acids and their impact on infant formula health benefits based on published literature.
Article
Veterinary Sciences
Hayami Kudo, Tomochika Sugiura, Seiya Higashi, Kentaro Oka, Motomichi Takahashi, Shigeru Kamiya, Yutaka Tamura, Masaru Usui
Summary: The study revealed that the early postpartum microbiota changes in primiparous cows have a significant impact on the development of endometritis. Bacteria such as Trueperella, Enterococcus, and Bifidobacterium showed specific abundance changes at different time points in healthy and endometritis-diagnosed cows. Building an environment with an increase in beneficial bacteria, such as Lactobacillus, may help reduce the abundance of pathogenic bacteria like Trueperella and control endometritis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Marine & Freshwater Biology
Mahanama Geegana Gamage Awanthi, Natsuki Nakasone, Hirosuke Oku, Kanefumi Kitahara, Michihiro Ito, Atsuko Tanaka, Yoichi Sato, Yuichiro Numata, Teruko Konishi
Summary: The structure of cell wall polysaccharide in Cladosiphon okamuranus varies significantly depending on geographic location, but relatively similar fucoidan can be obtained from any location in Okinawa Prefecture during peak time.
PHYCOLOGICAL RESEARCH
(2023)
Article
Plant Sciences
Houssem Kahli, Hana Sbartai, Touria Cohen-Bouhacina, Jacques Bourguignon
Summary: This study compares the resistance of halophytes to cadmium stress and finds that they can restore cellular homeostasis by activating antioxidant defense mechanisms and accumulate high amounts of Cd in leaves. These plants may be good candidates for Cd phytoextraction in the presence of low pollutant concentrations.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Lauren Zink, Carolyn Simonis, Steve Wiseman, Gregory G. Pyle
Summary: This study used an in vitro gut sac technique to investigate the translocation of cadmium across the gut barrier of fathead minnows following the simulated ingestion of cadmium, microplastics, or their complexed mixture. The presence of microplastics was found to be protective of dietary cadmium uptake. This work highlights the importance of considering dietary uptake and the role of microplastics acting as a vector for cadmium in aquatic environments.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Soumyadeep Sarkar, John T. Melchior, Hayden R. Henry, Farooq Syed, Raghavendra G. Mirmira, Ernesto S. Nakayasu, Thomas O. Metz
Summary: This paper discusses the potential role of GDF15 in preventing insulitis and protecting pancreatic beta cells against cellular stress. Although GDF15 shows promise as a potential intervention, further development is needed, and challenges such as anorexia and short half-life need to be addressed.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2022)
Article
Biology
Jiaqi Fu, Mowei Zhou, Marina A. Gritsenko, Ernesto S. Nakayasu, Lei Song, Zhao-Qing Luo
Summary: The intracellular pathogen Legionella pneumophila delivers effectors into host cells to modulate host energy metabolism, contributing to its virulence mechanism.
Article
Chemistry, Analytical
Sean M. Colby, Christine H. Chang, Jessica L. Bade, Jamie R. Nunez, Madison R. Blumer, Daniel J. Orton, Kent J. Bloodsworth, Ernesto S. Nakayasu, Richard D. Smith, Yehia M. Ibrahim, Ryan S. Renslow, Thomas O. Metz
Summary: DEIMoS is a Python tool for high-dimensional mass spectrometry data analysis that provides features such as feature detection, feature alignment, and calibration. It operates on N-dimensional data and can improve detection sensitivity and data alignment confidence while reducing artifacts in tandem mass spectra. The demonstration with metabolomics data shows the advantages of using DEIMoS in each data processing step.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Sarah C. Feid, Hanna E. Walukiewicz, Xiaoyi Wang, Ernesto S. Nakayasu, Christopher Rao, Alan J. Wolfe
Summary: Lysine acetylation inhibits translation by interfering with ribosome association, providing new insights into the mechanism and physiological effects of lysine acetylation.
Article
Immunology
Carla Elizabete Octaviano, Nadiellen E. Abrantes, Rosana Puccia
Summary: Extracellular vesicles (EVs) play an important role in cell-cell communication, cell wall remodeling, and fungal virulence, especially in Paracoccidioides spp. In this study, the role of fungal EVs in oxidative and nitrosative stress sensitivity, as well as macrophage inflammatory response, was investigated. The results showed that EVs could affect the sensitivity to stress and induce inflammatory mediators in macrophages, thereby exacerbating murine pulmonary paracoccidioidomycosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Joon-Yong Lee, Hugh D. Mitchell, Meagan C. Burnet, Ruonan Wu, Sarah C. Jenson, Eric D. Merkley, Ernesto S. Nakayasu, Carrie D. Nicora, Janet K. Jansson, Kristin E. Burnum-Johnson, Samuel H. Payne
Summary: Metaproteomics is increasingly used for high-throughput characterization of proteins in complex environments, and the creation of a sample-specific protein sequence database is crucial for accurate analysis. This study presents a de novo peptide sequencing approach to identify sample composition directly from metaproteomic data, using a deep learning model trained on diverse bacteria. The results demonstrate the potential of this method as an alternative and complementary approach to construct sample-specific protein databases, particularly in the absence of matched metagenomes.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Microbiology
Bikash Shakya, Geoffrey Kimiti Kilili, Ling Wang, Ernesto S. Nakayasu, Douglas J. LaCount
Summary: Exported proteins play important roles in creating a favorable environment for Plasmodium replication. This study identified several putative exported P. falciparum proteins that interact with erythrocyte cytoskeletal proteins through biochemical screening and affinity purification methods.
Review
Endocrinology & Metabolism
Rebecca S. Aguirre, Abhishek Kulkarni, Matthew W. Becker, Xiaoyong Lei, Soumyadeep Sarkar, Sasanka Ramanadham, Edward A. Phelps, Ernesto S. Nakayasu, Emily K. Sims, Raghavendra G. Mirmira
Summary: This review examines the impact of extracellular vesicles (EVs) released by pancreatic islet beta cells on the development of type 1 diabetes (T1D). The formation and cargo of EVs play important roles in the pathogenesis of T1D and have the potential to serve as biomarkers or therapeutic candidates.
MOLECULAR METABOLISM
(2022)
Article
Mycology
Anderson Messias Rodrigues, Ferry Hagen, Rosana Puccia, Rosane Christine Hahn, Zoilo Pires de Camargo
Summary: Paracoccidioidomycosis (PCM) is a group of diseases caused by Paracoccidioides species. The genus has expanded to include seven species. Classic PCM affects the human lungs and may lead to systemic granulomatous disease, while PCM loboi and PCM ceti are observed as subcutaneous mycoses in humans and dolphins. Recognizing species boundaries in Paracoccidioides is crucial for understanding its ecology, evolution, clinical features, and strategies to mitigate PCM.
Review
Microbiology
Sneha P. Couvillion, Katie E. Mostoller, Janet E. Williams, Ryan M. Pace, Izabel L. Stohel, Haley K. Peterson, Carrie D. Nicora, Ernesto S. Nakayasu, Bobbie-Jo M. Webb-Robertson, Mark A. McGuire, Michelle K. McGuire, Thomas O. Metz
Summary: There is increasing interest in understanding the functional role of milk-associated microbiota due to their impact on host health and phenotype. Mastitis, a painful inflammation of the mammary gland, affects lactating humans and animals and has significant clinical and economic consequences. Alterations in milk microbial community composition have been observed in mastitis, suggesting a potential causal relationship with the host phenotype. Multi-omic approaches can provide mechanistic insights into how the milk microbiome modulates host physiology, informing strategies for mastitis prevention and treatment. This paper reviews existing studies utilizing omics approaches to investigate the role of the milk microbiome in mastitis, and discusses the strengths and challenges associated with different omics techniques.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Rosana Puccia, Carlos Peleschi Taborda
Summary: This review discusses Dr. Luiz Rodolpho Raja Gabaglia Travassos' scientific contributions to paracoccidioidomycosis. The review is co-written by Rosana Puccia and Dr. Carlos P. Taborda, who share personal anecdotes alongside recognizing Dr. Travassos' pioneering papers and scientific insights. The focus is on his contributions in biochemistry, immunology, cell biology, and molecular biology, particularly in relation to the Paracoccidioides antigen gp43. Dr. Travassos' impact on the scientific community is evident through numerous quality publications, as well as the lasting influence he had on those who worked with him.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Sneha P. Couvillion, Robert E. Danczak, Dan Naylor, Montana L. Smith, Kelly G. Stratton, Vanessa L. Paurus, Kent J. Bloodsworth, Yuliya Farris, Darren J. Schmidt, Rachel E. Richardson, Lisa M. Bramer, Sarah J. Fansler, Ernesto S. Nakayasu, Jason E. McDermott, Thomas O. Metz, Mary S. Lipton, Janet K. Jansson, Kirsten S. Hofmockel
Summary: Microbial lipids and metabolites in the soil play a critical role in regulating the microbial response to stress, especially during drying-rewetting cycles. This study revealed specific changes in lipids and metabolites that are indicative of stress adaptation, substrate use, and cellular recovery during soil drying and subsequent rewetting. The results underscore the importance of the soil lipidome as a robust indicator of microbial community responses.
Review
Pharmacology & Pharmacy
Ivo Diaz Ludovico, Soumyadeep Sarkar, Emily Elliott, Suvi M. Virtanen, Iris Erlund, Sasanka Ramanadham, Raghavendra G. Mirmira, Thomas O. Metz, Ernesto S. Nakayasu
Summary: This article provides an overview of the role of fatty acids in type 1 diabetes and their potential as therapeutic targets. It discusses the regulation of lipotoxicity and inflammatory response, the impact of diet on fatty acid availability and disease development, and avenues for further exploration.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2023)
Article
Cell Biology
Ernesto S. Nakayasu, Lisa M. Bramer, Charles Ansong, Athena A. Schepmoes, Thomas L. Fillmore, Marina A. Gritsenko, Therese R. Clauss, Yuqian Gao, Paul D. Piehowski, Bryan A. Stanfill, Dave W. Engel, Daniel J. Orton, Ronald J. Moore, Wei-Jun Qian, Salvatore Sechi, Brigitte I. Frohnert, Jorma Toppari, Anette-G. Ziegler, Ake Lernmark, William Hagopian, Beena Akolkar, Richard D. Smith, Marian J. Rewers, Bobbie-Jo M. Webb-Robertson, Thomas O. Metz, Teddy Study Grp
Summary: Type 1 diabetes (T1D) is caused by autoimmune destruction of b cells. The insufficient availability of biomarkers hinders understanding of the disease. This study identifies and validates biomarkers predictive of T1D development through plasma proteomics, highlighting the pathways affected during T1D progression.
CELL REPORTS MEDICINE
(2023)
Article
Medicine, General & Internal
Ernesto S. Nakayasu, Marina A. Gritsenko, Young-Mo Kim, Jennifer E. Kyle, Kelly G. Stratton, Carrie D. Nicora, Nathalie Munoz, Kathleen M. Navarro, Daniel Claborne, Yuqian Gao, Karl K. Weitz, Vanessa L. Paurus, Kent J. Bloodsworth, Kelsey A. Allen, Lisa M. Bramer, Fernando Montes, Kathleen A. Clark, Grant Tietje, Justin Teeguarden, Kristin E. Burnum-Johnson
Summary: This study utilizes multi-omics analysis of biofluids to uncover simultaneous regulatory signatures during intense physical activity, providing insights into altered susceptibility to respiratory infections.
MILITARY MEDICAL RESEARCH
(2023)