Article
Virology
Hu Dong, Yuanlu Lu, Yun Zhang, Suyu Mu, Nan Wang, Ping Du, Xiaoying Zhi, Xiaobo Wen, Xiangxi Wang, Shiqi Sun, Yanming Zhang, Huichen Guo
Summary: This study identified thermostable FMDV mutants by high-temperature screening, with an important alanine-to-threonine mutation at position 13 in the VP1 protein for capsid stability. These mutants showed improved stability and immunogenicity compared to the wild-type virus, potentially aiding in the development of a stable vaccine against FMDV serotype O.
JOURNAL OF VIROLOGY
(2021)
Article
Biology
Joseph Newman, David J. Rowlands, Tobias J. Tuthill
Summary: This study compared the characteristics of recombinant assembly and disassembly pentamers of picornavirus, specifically FMDV. Assembly pentamers sedimented faster and had higher reactivity with anti-VP4 antibodies compared to the disassembly pentamers. However, the reactivity with other antibodies was similar between the two pentamers, suggesting that major antigenic features may be preserved between assembly pentamers containing VP4 and disassembly pentamers lacking VP4.
Article
Multidisciplinary Sciences
Liya Hu, Wilhelm Salmen, Rong Chen, Yi Zhou, Frederick Neill, James E. Crowe, Robert L. Atmar, Mary K. Estes, B. V. Venkataram Prasad
Summary: This study reveals the structure and features of human norovirus GII.4 virus-like particles using X-ray crystallography and cryo-EM. It uncovers the adaptability and stability of the capsid protein VP1, as well as its potential antigen presentation mechanism, providing valuable insights for vaccine development.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Haozhou Li, Aldo Dekker, Shiqi Sun, Alison Burman, Jeroen Kortekaas, Michiel M. Harmsen
Summary: The efficacy of foot-and-mouth disease (FMD) vaccine depends on the content of intact and empty viral particles. Capsid-specific VHH antibodies can be used to quantify intact capsids in the vaccine, improving efficacy and stability. These antibodies serve as excellent tools for monitoring the quantity and stability of intact capsids during the vaccine manufacturing process.
Article
Biochemical Research Methods
Ming Yang, Boitumelo Mudabuka, Codey Dueck, Wanhong Xu, Kabo Masisi, Elliot M. Fana, Christopher Mpofu, Charles Nfon
Summary: Foot-and-mouth disease (FMD) is a highly contagious disease affecting livestock production, especially in endemic countries. Rapid and accurate detection tests for FMDV serotypes SAT 1 and SAT 3 have been developed, with high specificity and diagnostic sensitivity.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Biochemical Research Methods
Jingxuan Qiu, Tianyi Qiu, Qingli Dong, Dongpo Xu, Xiang Wang, Qi Zhang, Jing Pan, Qing Liu
Summary: A computational model was designed to rapidly predict the antigenicity of FMDV, showing high accuracy and AUC value. The model successfully evaluated the annual antigenicity coverage of historical vaccine strains and was extended to select potential broad-spectrum vaccine candidates among historical non-redundant strains.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2021)
Article
Chemistry, Multidisciplinary
Rajkumar Misra, Thangavel Vijayakanth, Linda J. W. Shimon, Lihi Adler-Abramovich
Summary: In this study, the atomistic details of helical secondary structure formation and super helical assembly of two heptapeptides composed of sequentially arranged Phe residues were revealed using helix promoting Aib residues.
CHEMICAL COMMUNICATIONS
(2022)
Article
Immunology
Junhong Hao, Chaochao Shen, Nannan Wei, Minghao Yan, Xuegang Zhang, Guowei Xu, Dajun Zhang, Jing Hou, Weijun Cao, Ye Jin, Keshan Zhang, Haixue Zheng, Xiangtao Liu
Summary: Foot-and-mouth disease virus antagonizes the activation of the IRF3/IFN-β signaling pathway via TPL2 for immune escape and enhanced virus replication. TPL2 deficiency leads to reduced expression of interferon-stimulated genes and interferon regulatory factors, resulting in increased virus replication.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Jonathan Arzt, Ian H. Fish, Miranda R. Bertram, George R. Smoliga, Ethan J. Hartwig, Steven J. Pauszek, Lauren Holinka-Patterson, Fayna C. Diaz-San Segundo, Tatjana Sitt, Elizabeth Rieder, Carolina Stenfeldt
Summary: Research showed that cattle can be simultaneously infected with two different serotypes of foot-and-mouth disease virus (FMDV), with different outcomes depending on the timing of exposure to the viruses. Dominant interserotypic recombinant FMDVs were discovered in upper respiratory tract samples of superinfected animals, indicating the potential role of persistently infected FMDV carriers in generating novel viral strains.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Zhidong Zhang, Ryan Waters, Yanmin Li
Summary: Foot-and-mouth disease virus (FMDV) infection causes epithelial cell lesions and muscle-associated lesions in cloven-hoofed animals, leading to high mortality. Current knowledge about the non-epithelial tropism of FMDV remains limited.
VETERINARY MICROBIOLOGY
(2021)
Article
Biology
Florian Mattenberger, Victor Latorre, Omer Tirosh, Adi Stern, Ron Geller
Summary: This study comprehensively defines the effects of mutations on the capsid of a human picornavirus, identifying key structural and sequence determinants that predict mutational fitness effects. It also introduces a bioinformatic approach for identifying host proteins targeted by viral proteases based on capsid-encoded protease cleavage sites, shedding light on important aspects of viral biology, evolution, and host interactions.
Article
Biochemical Research Methods
Yanli Yang, Ming Li, Yi Zhao, Xuan Lin, Zhiguo Su, Fei Xin, Xiaojie Du, Kan Zheng, Rui Han, Yiping Pan, Sun He, Songping Zhang
Summary: This study found strong non-specific adsorption of type A/AKT III FMDV on TSK G40 0 0 SWXL column in HPSEC analysis. By adding specific amino acid residues and salt additives, this adsorption can be suppressed. The optimized mobile phase conditions allow accurate quantification of two serotypes of FMDV with low relative standard deviation (RSD).
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Veterinary Sciences
Eva Perez-Martin, Brianna Beechler, Fuquan Zhang, Katherine Scott, Lin-Mari De Klerk-Lorist, Georgina Limon, Brian Dugovich, Simon Gubbins, Arista Botha, Robyn Hetem, Louis van Schalkwyk, Nicholas Juleff, Francois F. Maree, Anna Jolles, Bryan Charleston
Summary: This study revealed for the first time that African buffalo are systemically affected by foot-and-mouth disease (FMD) virus and the clinical characteristics of FMD in buffalo include transient pyrexia. Despite the lack of lesions, African buffalo infection is characterized by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses, and high transmissibility.
VETERINARY RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Yue Luo, Junjun Cheng, Zhanying Hu, Haiqun Ban, Shuo Wu, Nicky Hwang, John Kulp, Yuhuan Li, Yanming Du, Jinhong Chang, Usha Viswanathan, Ju-Tao Guo
Summary: The study identified critical Cp amino acid residues for capsid assembly, pgRNA encapsidation, and resistance to CpAMs through systematic mutagenesis analysis. Specific residues such as W102 and Y132 are crucial for capsid assembly, while other residues affect pgRNA encapsidation. Several mutant Cp support high levels of DNA replication but show strong resistance to CpAMs.
ANTIVIRAL RESEARCH
(2021)
Article
Biophysics
Heba A. Hussein, Rasha Mohamed El Nashar, Ibrahim M. El-Sherbiny, Rabeay Y. A. Hassan
Summary: The SAT-2VIP sensor shows excellent selectivity and sensitivity, with the ability to rapidly detect foot-and-mouth disease virus in clinical samples without the need for treatment or equipped labs. This supports on-site point of care diagnostics for SAT-2 virus.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Stefan Bienert, Andrew Waterhouse, Tjaart A. P. de Beer, Gerardo Tauriello, Gabriel Studer, Lorenza Bordoli, Torsten Schwede
NUCLEIC ACIDS RESEARCH
(2017)
Article
Virology
Katherine A. Scott, Abhay Kotecha, Julian Seago, Jingshan Ren, Elizabeth E. Fry, David I. Stuart, Bryan Charleston, Francois F. Maree
JOURNAL OF VIROLOGY
(2017)
Article
Veterinary Sciences
D. D. Lazarus, G. T. Fosgate, O. L. van Schalkwyk, R. E. J. Burroughs, L. Heath, F. F. Maree, B. Blignaut, B. Reininghaus, A. Mpehle, O. Rikhotso, G. R. Thomson
PREVENTIVE VETERINARY MEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Frederic V. Stanger, Tjaart A. P. de Beer, David M. Dranow, Tilman Schirmer, Isabelle Phan, Christoph Dehio
Article
Immunology
Katherine A. Scott, N. M. Rathogwa, A. V. Capozzo, Francois F. Maree
Article
Virology
Elaine Vermaak, Francois F. Maree, Jacques Theron
Article
Multidisciplinary Sciences
Yannick Mahlich, Jonas Reeb, Maximilian Hecht, Maria Schelling, Tjaart Andries, Petrus De Beer, Yana Bromberg, Burkhard Rost
SCIENTIFIC REPORTS
(2017)
Article
Biochemical Research Methods
M. Chitray, S. Grazioli, T. Willems, T. Tshabalala, A. De Vleeschauwer, J. J. Esterhuysen, E. Brocchi, K. De Clercq, F. F. Maree
JOURNAL OF VIROLOGICAL METHODS
(2018)
Article
Biochemistry & Molecular Biology
Andrew Waterhouse, Martino Bertoni, Stefan Bienert, Gabriel Studer, Gerardo Tauriello, Rafal Gumienny, Florian T. Heer, Tjaart A. P. de Beer, Christine Rempfer, Lorenza Bordoli, Rosalba Lepore, Torsten Schwede
NUCLEIC ACIDS RESEARCH
(2018)
Article
Immunology
Caroline K. Glidden, Brianna Beechler, Peter Erik Buss, Bryan Charleston, Lin-Mari de Klerk-Lorist, Francois Frederick Maree, Timothy Muller, Eva Perez-Martin, Katherine Anne Scott, Ockert Louis van Schalkwyk, Anna Jolles
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Veterinary Sciences
D. D. Lazarus, O. L. van Schalkwyk, R. E. J. Burroughs, A. Mpehle, B. Reininghaus, O. Rikhotso, L. Heath, F. F. Maree, B. Blignaut, G. T. Fosgate
PREVENTIVE VETERINARY MEDICINE
(2018)
Article
Multidisciplinary Sciences
Abhay Kotecha, Eva Perez-Martin, Yongjie Harvey, Fuquan Zhang, Serban L. Ilca, Elizabeth E. Fry, Ben Jackson, Francois Maree, Katherine Scott, Corey W. Hecksel, Michiel M. Harmsen, Valerie Mioulet, Britta Wood, Nick Juleff, David I. Stuart, Bryan Charleston, Julian Seago
SCIENTIFIC REPORTS
(2018)
Article
Ecology
Miriam Casey-Bryars, Richard Reeve, Umesh Bastola, Nick J. Knowles, Harriet Auty, Katarzyna Bachanek-Bankowska, Veronica L. Fowler, Robert Fyumagwa, Rudovick Kazwala, Tito Kibona, Alasdair King, Donald P. King, Felix Lankester, Anna B. Ludi, Ahmed Lugelo, Francois F. Maree, Deogratius Mshanga, Gloria Ndhlovu, Krupali Parekh, David J. Paton, Brian Perry, Jemma Wadsworth, Satya Parida, Daniel T. Haydon, Thomas L. Marsh, Sarah Cleaveland, Tiziana Lembo
NATURE ECOLOGY & EVOLUTION
(2018)
Article
Microbiology
Tilman Schirmer, Tjaart A. P. de Beer, Stefanie Tamegger, Alexander Harms, Nikolaus Dietz, David M. Dranow, Thomas E. Edwards, Peter J. Myler, Isabelle Phan, Christoph Dehio
Summary: This study elucidates the expansion and diversification of FIC domains in the Bartonella Bep repertoire using X-ray crystallography, structural modeling, and phylogenetic analysis. The findings reveal remarkable functional plasticity of Beps primarily due to structural changes in the substrate pocket and the target dock. These insights may guide future structure-function analyses of these highly versatile FIC domains.
Article
Statistics & Probability
Vinny Davies, Richard Reeve, William T. Harvey, Francois F. Maree, Dirk Husmeier
COMPUTATIONAL STATISTICS
(2017)