Article
Biochemistry & Molecular Biology
Cheng-Long Huang, Liu-Ling Xiao, Min Xu, Jun Li, Shu-Fen Li, Cui-Song Zhu, Yu-Li Lin, Rui He, Xi Li
Summary: Adipocytes and immune cells are crucial for adipose tissue development, with specific roles in different adipose tissue depots. Chemerin deficiency in adipose tissue can lead to altered regulation of adipogenesis and macrophage infiltration, potentially influencing metabolic disorders such as obesity.
Article
Cell Biology
Anh Cuong Hoang, Haidong Yu, Tamas Roeszer
Summary: The study identified gene networks in developing iWAT and BAT in mice, showing similarities and differences in gene expression related to immune responses and antiviral factors. It also revealed a metabolic shift in postnatal iWAT from thermogenesis and lipolysis towards fat storage. Additionally, differences were found in the signals for thermogenic fat differentiation between early postnatal life and adulthood.
Article
Biochemistry & Molecular Biology
Jiangang Han, Xiaojie Li, Benmeng Liang, Sijia Ma, Yabin Pu, Fuqing Yu, Jian Lu, Yuehui Ma, David E. Machugh, Lin Jiang
Summary: This study describes the isolation of adipose-derived stem cells (ADSCs) from sheep embryonic fat tail tissue, which have high self-renewal capacity, multilineage differentiation, and excellent adipogenic ability. Transcriptome analysis reveals the involvement of multiple transcription factors in the early events of adipogenesis, as well as genes related to lipid generation, energy metabolism, and myogenesis. Comparative transcriptome analysis across species demonstrates enhanced basal metabolic ability in differentiating ovine ADSCs, and identifies a set of evolutionarily conserved genes regulating adipogenesis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Genetics & Heredity
Dan Jiao, Kaixi Ji, Hu Liu, Wenqiang Wang, Xiukun Wu, Jianwei Zhou, Yunsheng Zhang, Huitong Zhou, Jon G. H. Hickford, Allan A. Degen, Guo Yang
Summary: There are differences in cold tolerance between Hu and Altay sheep. Under cold exposure of the lambs, UCP1-dependent thermogenesis and calcium- and cAMP-signaling pathways were activated, and different fat tissues were activated in Hu and Altay lambs. Several candidate genes involved in thermogenesis including UCP1, ADRB3, ADORA2A, ATP2A1, RYR1 and IP6K1 were identified.
Article
Biotechnology & Applied Microbiology
Meilin Jin, Xiaojuan Fei, Taotao Li, Zengkui Lu, Mingxing Chu, Ran Di, Xiaoyun He, Xiangyu Wang, Caihong Wei
Summary: This study identified key genes involved in adipogenesis metabolism in Hu sheep and Tibetan sheep, providing novel insights into metabolic molecules in sheep fat tails.
Article
Zoology
Chun-Long Yan, Jun Lin, Yuan-Yuan Huang, Qing-Shan Gao, Zheng-Yu Piao, Shou-Li Yuan, Li Chen, Xue Ren, Rong-Cai Ye, Meng Dong, Han-Lin Zhang, Hui-Qiao Zhou, Xiao-Xiao Jiang, Wan-Zhu Jin, Xu-Ming Zhou, Chang-Guo Yan
Summary: Environmental temperature plays a significant role in shaping genetic variation in domestic cattle, with the PRDM16 gene identified as crucial in maintaining brown adipocyte formation in northern cattle for cold tolerance.
ZOOLOGICAL RESEARCH
(2022)
Article
Plant Sciences
Shuaishuai Zhang, Tingyu Shan, Jingyao Xu, Liqiang Zhao, Jiawen Wu
Summary: This study identified potential key genes involved in the biosynthesis of steroidal glycoalkaloids (SGAs) in Solanum khasianum C. B. Clarke through comprehensive RNA sequencing analysis. Additionally, the presence of Solasodine, a constituent involved in SGA biosynthesis, was detected using high-performance liquid chromatography. This is the first report of comparative transcriptome analysis identifying valuable genes potentially involved in SGA biosynthesis in this species.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Genetics & Heredity
Hui Wang, Jincheng Zhong, Jikun Wang, Zhixin Chai, Chengfu Zhang, Jinwei Xin, Jiabo Wang, Xin Cai, Zhijuan Wu, Qiumei Ji
Summary: This study sequenced the heart tissues of high-altitude adapted yaks and related cattle breeds, revealing differentially expressed protein-coding and non-coding transcripts associated with immune system, endocrine system, and lipid metabolism. The construction of competitive endogenous RNA networks and hypoxic adaptation-related networks identified novel and promising candidate genes for regulating high-altitude adaptation in the heart.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Tainara C. Michelotti, Brent R. Kisby, Lauryn S. Flores, Alexandra P. Tegeler, Mohamed Fokar, Chiquito Crasto, Bruno C. Menarim, Shavahn C. Loux, Clarissa Strieder-Barboza
Summary: This study reveals the cellular diversity differences between visceral and subcutaneous adipose tissue in dairy cows at the single-nuclei level through snRNA-seq analysis. The visceral adipose tissue showed higher activation of immune and inflammatory response pathways and had a pro-inflammatory profile.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Plant Sciences
Yansong Zhu, Yicheng Wang, Huiyan Jiang, Wenjun Liu, Shuhui Zhang, Xukai Hou, Susu Zhang, Nan Wang, Rui Zhang, Zongying Zhang, Xuesen Chen
Summary: Pear fruit stone cells, formed by the deposition of lignin, greatly affect the fruit's edibility. Through analyzing pear flesh samples, we identified genes related to stone cell formation and two modules related to lignin. Experimental validation revealed the positive regulation of PbMYB61 gene on stone cell lignin formation.
Article
Multidisciplinary Sciences
Isabelle Cassar-Malek, Lise Pomies, Anne de la Foye, Jeremy Tournayre, Celine Boby, Jean-Francois Hocquette
Summary: This study aims to investigate the effect of emotional and physical stress on the transcriptome of meat-producing animals. The results showed that exposure to stress led to differential gene expression in muscles, involving regulators of energy metabolism, hypoxia response, and inflammatory cytokines. This study provides insight into the molecular processes involved in the response to stress and their impact on meat quality.
Article
Plant Sciences
Sudthana Khlaimongkhon, Sriprapai Chakhonkaen, Keasinee Tongmark, Numphet Sangarwut, Natjaree Panyawut, Thiwawan Wasinanon, Kannika Sikaewtung, Samart Wanchana, Chareerat Mongkolsiriwatana, Julapark Chunwonges, Amorntip Muangprom
Summary: Rice is a crucial food crop for nearly half of the world's population, and its grain yields are affected by temperature changes. Thermosensitive genic male sterility (TGMS) rice is sterile at high temperatures and fertile at low temperatures, making it a good model for studying the effects of temperature on male development. Transcriptomic analysis revealed differential gene expression in TGMS rice panicles under sterile and fertile conditions, with some genes showing unique responses to temperature in TGMS plants compared to wild-type rice. These findings provide insights into the molecular mechanisms controlling TGMS and rice responses to temperature alterations.
Article
Endocrinology & Metabolism
Yanbing Zhou, Ziye Xu, Liyi Wang, Defeng Ling, Qiuyun Nong, Jintang Xie, Xiaodong Zhu, Tizhong Shan
Summary: This study investigated the effects of cold exposure on fatty acid composition and transcriptional profiles of subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in pigs lacking functional UCP1 gene. It was found that cold exposure led to a decrease in saturated fatty acid content in SAT, while specific fatty acids in VAT were decreased without affecting total SFA content. The transcriptional changes induced by cold exposure were more limited in VAT, mainly involving fat cell differentiation and cell proliferation. Additionally, cold treatment increased the levels of organic acids in plasma, suggesting a regulatory role of organic acids in lipid and fatty acid metabolism in white adipose tissue (WAT) during cold exposure.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Food Science & Technology
Hai -Bo Xiong, Xiao-Hong Zhou, Wen-Liang Xiang, Min Huang, Zi-Xi Lin, Jie Tang, Ting Cai, Qing Zhang
Summary: D-limonene is a natural flavor additive commonly used in the food industry. It has the ability to disrupt the balance of cell phenotypic parameters, thus inhibiting the growth of Candida tropicalis. This study aimed to investigate the molecular mechanism behind this inhibition by analyzing transcriptome and phenotypic parameters. The results showed that D-limonene affected various cellular processes, leading to metabolic disorders in Candida tropicalis, including compromised cell wall and membrane integrity, disrupted energy production, and increased stress response. These findings contribute to a better understanding of the anti-C. tropicalis mechanism of D-limonene and its potential applications in food preservation and preventive medicine.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Bonggyu Min, Kkotnim Kim, Vladimir Li, Seoae Cho, Heebal Kim
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Microbiology
Woori Kwak, Young-Hyun Han, Donghyeok Seol, Hyaekang Kim, Hyeonju Ahn, Misun Jeong, Jaeku Kang, Heebal Kim, Tae Hyun Kim
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biology
Lukas Ded, Jae Yeon Hwang, Kiyoshi Miki, Huanan F. Shi, Jean-Ju Chung
Article
Agriculture, Dairy & Animal Science
Juwan Kim, Chul Lee, DongAhn Yoo, Heebal Kim
Summary: Mudskippers are amphibious fish that share similar genetic adaptations with ancient tetrapods for land adaptation, especially in immune response and mitochondrial oxidative phosphorylation. Comparative genomic analysis reveals convergent genetic adaptations in mudskippers and tetrapods, shedding light on the evolutionary history of water-to-land transition in vertebrates.
Article
Medicine, Legal
Soyeon Bae, Sohyoung Won, Heebal Kim
Summary: This study identified 160 SNPs with high allele frequencies for paternity testing in Koreans by filtering candidate SNPs from the Ansan-Ansung cohort data and calculating likelihood ratios. Validation using Twin-Family cohort data showed accurate distinction between paternity and non-paternity when using the selected 160 SNPs for calculation.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Arang Rhie, Shane A. McCarthy, Olivier Fedrigo, Joana Damas, Giulio Formenti, Sergey Koren, Marcela Uliano-Silva, William Chow, Arkarachai Fungtammasan, Juwan Kim, Chul Lee, Byung June Ko, Mark Chaisson, Gregory L. Gedman, Lindsey J. Cantin, Francoise Thibaud-Nissen, Leanne Haggerty, Iliana Bista, Michelle Smith, Bettina Haase, Jacquelyn Mountcastle, Sylke Winkler, Sadye Paez, Jason Howard, Sonja C. Vernes, Tanya M. Lama, Frank Grutzner, Wesley C. Warren, Christopher N. Balakrishnan, Dave Burt, Julia M. George, Matthew T. Biegler, David Iorns, Andrew Digby, Daryl Eason, Bruce Robertson, Taylor Edwards, Mark Wilkinson, George Turner, Axel Meyer, Andreas F. Kautt, Paolo Franchini, H. William Detrich, Hannes Svardal, Maximilian Wagner, Gavin J. P. Naylor, Martin Pippel, Milan Malinsky, Mark Mooney, Maria Simbirsky, Brett T. Hannigan, Trevor Pesout, Marlys Houck, Ann Misuraca, Sarah B. Kingan, Richard Hall, Zev Kronenberg, Ivan Sovi, Christopher Dunn, Zemin Ning, Alex Hastie, Joyce Lee, Siddarth Selvaraj, Richard E. Green, Nicholas H. Putnam, Ivo Gut, Jay Ghurye, Erik Garrison, Ying Sims, Joanna Collins, Sarah Pelan, James Torrance, Alan Tracey, Jonathan Wood, Robel E. Dagnew, Dengfeng Guan, Sarah E. London, David F. Clayton, Claudio Mello, Samantha R. Friedrich, Peter Lovell, Ekaterina Osipova, Farooq O. Al-Ajli, Simona Secomandi, Heebal Kim, Constantina Theofanopoulou, Michael Hiller, Yang Zhou, Robert S. Harris, Kateryna D. Makova, Paul Medvedev, Jinna Hoffman, Patrick Masterson, Karen Clark, Fergal Martin, Kevin Howe, Paul Flicek, Brian P. Walenz, Woori Kwak, Hiram Clawson, Mark Diekhans, Luis Nassar, Benedict Paten, Robert H. S. Kraus, Andrew J. Crawford, M. Thomas P. Gilbert, Guojie Zhang, Byrappa Venkatesh, Robert W. Murphy, Klaus-Peter Koepfli, Beth Shapiro, Warren E. Johnson, Federica Di Palma, Tomas Marques-Bonet, Emma C. Teeling, Tandy Warnow, Jennifer Marshall Graves, Oliver A. Ryder, David Haussler, Stephen J. O'Brien, Jonas Korlach, Harris A. Lewin, Kerstin Howe, Eugene W. Myers, Richard Durbin, Adam M. Phillippy, Erich D. Jarvis
Summary: The Vertebrate Genome Project and the international Genome 10K consortium have collaborated to generate high-quality genome assemblies for 16 species representing six major vertebrate lineages, leading to new biological discoveries. Long-read sequencing technologies are essential for maximizing genome quality, and addressing complex repeats and haplotype heterozygosity are crucial for reducing assembly errors and improving completeness of reference genomes. The lessons learned from this project have paved the way for the Vertebrate Genomes Project (VGP), an international effort to generate high-quality, complete reference genomes for all known vertebrate species.
Article
Multidisciplinary Sciences
Bo-Hye Nam, Hyaekang Kim, Donghyeok Seol, Heebal Kim, Eun Soo Noh, Eun Mi Kim, Jae Koo Noh, Young-Ok Kim, Jung Youn Park, Woori Kwak
Summary: Continuous monitoring of genetic status is crucial for preserving the genetic resource of wild populations. This study used GBS to sequence Pacific abalone samples from around the Korean peninsula, revealing genetic diversity and population structure differences, as well as population-specific SNVs potentially associated with phenotype differences in Korean abalone populations.
Correction
Genetics & Heredity
Sohyoung Won, Jong-Eun Park, Ju-Hwan Son, Seung-Hwan Lee, Byeong Ho Park, Mina Park, Won-Chul Park, Han-Ha Chai, Heebal Kim, Jungjae Lee, Dajeong Lim
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Jae Yeon Hwang, Jamie Maziarz, Gunter P. Wagner, Jean-Ju Chung
Summary: The study investigated the molecular evolution and conservation of the CatSper channel in eutherians and marsupials. It found that CatSper proteins evolve slowly in marsupials and demonstrated the expression and function of CatSper in the American marsupial gray short-tailed opossum. A conserved IQ-like motif in CatSper zeta interacts with the pH-tuned Ca2+ sensor EFCAB9 to regulate CatSper activity.
Article
Cell Biology
Jae Yeon Hwang, Shoaib Nawaz, Jungmin Choi, Huafeng Wang, Shabir Hussain, Mehboob Nawaz, Francesc Lopez-Giraldez, Kyungjo Jeong, Weilai Dong, Jong-Nam Oh, Kaya Bilguvar, Shrikant Mane, Chang-Kyu Lee, Christopher Bystroff, Richard P. Lifton, Wasim Ahmad, Jean-Ju Chung
Summary: A novel DNAH2 gene variant was found to be closely associated with asthenozoospermia, and in mouse experiments, it was confirmed that DNAH2 deficiency leads to male infertility and affects sperm flagella development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jae Yeon Hwang, Huafeng Wang, Yonggang Lu, Masahito Ikawa, Jean-Ju Chung
Summary: In mammalian sperm cells, the regulation of spatiotemporal Ca2+ signaling relies on quadrilinear Ca2+ signaling nanodomains in the flagellar membrane. CatSpert, a C2 domain protein encoded by C2cd6, independently migrates to the flagella and serves as a major targeting component of the CatSper channel complex. CatSpert is essential for sperm hyperactivated motility and male fertility, and it targets the CatSper channel to the quadrilinear nanodomains in developing spermatids' flagella.
Article
Cell Biology
Jaekwang Jeong, Jae Hun Shin, Wenxue Li, Jun Young Hong, Jaechul Lim, Jae Yeon Hwang, Jean-Ju Chung, Qin Yan, Yansheng Liu, Jungmin Choi, John Wysolmerski
Summary: The lipid raft resident protein MAL2 contributes to the pathogenesis of malignancies, including breast cancer, through its effects on HER2 signaling and membrane stability. Physical interactions between HER2 and MAL2 in lipid rafts play a key role in maintaining low intracellular calcium concentrations and are enhanced in trastuzumab-resistant breast cancer cells, suggesting MAL2 as a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Qianhui Dou, Anton A. Turanov, Marco Mariotti, Jae Yeon Hwang, Huafeng Wang, Sang -Goo Lee, Joao A. Paulo, Sun Hee Yim, Stephen P. Gygi, Jean-Ju Chung, Vadim N. Gladyshev
Summary: TXNRD3 plays a critical role in male reproduction by controlling spermatogenesis through thiol redox regulation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yanhe Zhao, Huafeng Wang, Caroline Wiesehoefer, Naman B. Shah, Evan Reetz, Jae Yeon Hwang, Xiaofang Huang, Tse-en Wang, Polina V. Lishko, Karen M. Davies, Gunther Wennemuth, Daniela Nicastro, Jean-Ju Chung
Summary: The study visualizes the native in-cell 3D structure and higher-order organization of the CatSper calcium channels using cryo-electron tomography. The authors discover that the CatSper channels form long zigzag rows along the sperm tail and interact with other molecules. Moreover, the loss of EFCAB9-CATSPER zeta alters the architecture and interactions of the channels, disrupting flagellar movement in sperm.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jong-Nam Oh, Dongchan Son, Kwang-Hwan Choi, Jae Yeon Hwang, Dong-Kyung Lee, Seung-Hun Kim, Mingyun Lee, Jinsol Jeong, Gyung Cheol Choe, Chang-Kyu Lee