Article
Cell Biology
Yan-Ping Guo, Zhi-Feng Wang, Na Li, Qian-Qian Lei, Qiong Cheng, Li-Gang Shi, Sheng-Li Zhou, Xin-Hua Wang, Yang Sun, Ling-Fei Kong
Summary: This study found that lncRNA HOTAIR regulates RCC angiogenesis through the miR-126/EGFL7 axis, providing a new perspective on the molecular pathways of angiogenesis in RCC development and potentially serving as therapeutic targets for RCC treatment.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Medical Laboratory Technology
Goncalo Outeiro-pinho, Daniela Barros-silva, Filipa Moreira-silva, Joao Lobo, Isa Carneiro, Antonio Morais, Eduarda p. Martins, Celine s. Goncalves, Bruno m. Costa, Margareta p. Correia, Rui Henrique, Carmen Jeronimo
Summary: MiR-30a/c-5p is downregulated and associated with aggressiveness in ccRCC. Downregulation of miR-30a/c-5p is accompanied by increased promoter methylation, which can be restored by decitabine treatment. MiR-30a/c-5p not only decreases cell aggressiveness, but also upregulates epithelial markers and downregulates EMT-promoting players.
TRANSLATIONAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Haifeng Gao, Jiajia Xu, Fen Qiao, Liangjun Xue
Summary: The study found that in gastric cancer, hsa_circ_0000144 exerts inhibitory effects on oxaliplatin resistance, proliferation, and metastasis through regulating the miR-502-5p/ADAM9 axis.
ONCOTARGETS AND THERAPY
(2021)
Article
Cell Biology
Wenlong Xue, Qingqing Zhang, Ying Chen, Yichun Zhu
Summary: This study investigated the mechanism of hydrogen sulfide (H2S) regulation of miR-126-3p levels under high-glucose conditions. The results showed that H2S can increase miR-126-3p levels by decreasing methylation levels through down-regulating the DNMT1 protein level, thus improving angiogenesis impaired by high glucose.
Article
Biochemistry & Molecular Biology
Lu Qi, Lu Wang, Fuyao Song, Zhenhua Ding, Ying Zhang
Summary: This study identified miR-4469 as a tumor suppressor in colorectal cancer (CRC) and found that its regulatory mechanism mainly involves the infiltration of inflammatory cells in cancer tissues.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Gastroenterology & Hepatology
Bin Sun, Weidan Ji, Chunying Liu, Xuejing Lin, Lei Chen, Haihua Qian, Changqing Su
Summary: miR-2392 is significantly down-regulated in HCC and functions as a tumor suppressor by targeting JAG2 to inhibit proliferation, clonogenicity, mobility, spheroid formation, and maintenance of CSC-like characteristics in HCC cells. CTCs from HCC patients with lower serum miR-2392 levels exhibit stronger spheroid formation ability, and a negative correlation between miR-2392 levels in serum and the number of CTC spheroids has been observed. JAG2 is highly expressed in HCC and is associated with poor prognosis and patient survival.
LIVER INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Bo Liu, Rui Wang, Hongyan Liu
Summary: The study revealed that miR-126-5p was downregulated while ADAM9 was upregulated in NSCLC, and negatively regulating ADAM9 could increase the sensitivity of NSCLC to cisplatin.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Cell Biology
Ziqian Yan, Zhimei Sheng, Yuanhang Zheng, Ruijun Feng, Qinpei Xiao, Lihong Shi, Hongli Li, Chonggao Yin, Hao Luo, Chong Hao, Wenhao Wang, Baogang Zhang
Summary: Studies compared the effects of CAFs-derived exosomes and NFs-derived exosomes on breast cancer cells, finding that CAFs-derived exosomes had a stronger enhancing effect on migration and invasion.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Sujin Choi, Soonchul Lee, Young-Hoon Han, Junwon Choi, Isaac Kim, Jusung Lee, Hyun-Ju An
Summary: miR-31-3p is expressed at lower levels in prostate cancer tissues compared to adjacent normal tissues, and it plays a tumor-suppressive role by inducing apoptosis and decreasing cell proliferation, migration, invasiveness. GABBR2 is identified as a direct target of miR-31-3p.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Wei Zhao, Yanli Liu, Cairui Li, Zhijian Yin, Yi Liu, Meixia An
Summary: Diabetic retinopathy (DR) is the main cause of blindness in middle-aged and elderly people. Understanding the pathogenesis of proliferative diabetic retinopathy (PDR) can help develop treatments. This study investigated the involvement of the lncRNA MALAT1/miR-126-5p axis in modulating PDR progression. The results showed that MALAT1 promotes PDR by inhibiting miR126-5p and inducing REC proliferation, migration, and angiogenesis.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Xuyang Li, Zhan Zhang, Aipeng Li, Yubo Hu
Summary: The study found that PPF pre-treatment significantly reduced creatinine and urea nitrogen levels in the serum of BALB/c mice with RIRI, and decreased the apoptosis of HK-2 cells treated with H/R. Additionally, PPF pre-treatment markedly inhibited the expression of MALAT1 in both in vivo and in vitro models and upregulated miR-126-5p expression. MALAT1 could adsorb miR-126-5p to repress it and upregulated VEGFA.
JOURNAL OF GENE MEDICINE
(2021)
Article
Cell Biology
Chang-Liang Wu, Ti-Dong Shan, Yue Han, Yan Kong, Yuan-Bo Li, Xin-Gang Peng, Liang Shang, Pei-Ge Wang, Le-Ping Li
Summary: LINC00665 is significantly upregulated in CRC, promoting proliferation and inhibiting apoptosis through the regulation of key proteins. This suggests that LINC00665 may be an important biomarker and therapeutic target for colorectal cancer.
Article
Immunology
Kai-Xiang He, Lizhe Xu, Jin-Zhuo Ning, Fan Cheng
Summary: This study elucidated the mechanism underlying miR-195-5p's action in regulating pyroptosis in testicular ischemia/reperfusion injury. The results showed that miR-195-5p inhibited testicular IRI-induced pyroptosis by targeting PELP1 and attenuating the inflammatory response. This suggests that miR-195-5p may serve as a potential target for the development of therapies for testicular torsion.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qi Zou, Cheng Liu, Nan Hu, Wei Wang, Huaxue Wang
Summary: This study confirmed that miR-126 can regulate the differentiation of Th17/Treg, change the infiltration of inflammatory factors in peripheral blood, lung, liver and kidney of septic rats, alleviate MODS, and improve the organ function and prognosis of septic rats. However, using miR-126 inhibitor can lead to increased levels of inflammatory factors, aggravated multiple organ injury, and worse prognosis in septic rats.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Food Science & Technology
Fan Zhang, Xiu-Xiu Zhang, Jian-Guo Zhang, Kiran Thakur, Jesus Simal-Gandara, Miguel A. Prieto, Mohammad Rizwan Khan, Hui Cao, Zhao-Jun Wei
Summary: miRNA-6236-p5_4 plays a crucial role in the anticancer activity of AA in endometrial carcinoma. It is involved in regulating cell proliferation, cell cycle arrest, apoptosis, and migration. The PI3K-Akt, p53, Ras, and Rap1 signaling pathways, as well as CDK6, PIK3CB, and KRAS genes, are identified as key regulators in this process.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Oncology
Marianna Carrabotta, Maria Antonella Laginestra, Giorgio Durante, Caterina Mancarella, Lorena Landuzzi, Alessandro Parra, Francesca Ruzzi, Lisa Toracchio, Alessandra De Feo, Veronica Giusti, Michela Pasello, Alberto Righi, Pier-Luigi Lollini, Emanuela Palmerini, Davide Maria Donati, Maria Cristina Manara, Katia Scotlandi
Summary: CIC-DUX4-rearranged sarcomas, specifically Capicua-double homeobox 4 (CIC-DUX4)-rearranged sarcomas (CDS), are rare and aggressive tumors that require specific therapeutic approaches. This study identified a unique HMGA2/IGF2BP/IGF2/IGF1R/AKT/mTOR axis in CDS, indicating sensitivity to combined treatments with trabectedin and PI3K/mTOR inhibitors. The development of representative experimental models (PDXs and PDX-derived cell lines) has highlighted the potential of AKT/mTOR inhibitors and trabectedin in combating this lethal cancer.
Article
Oncology
Tommaso Balestra, Maria Cristina Manara, Maria Antonella Laginestra, Michela Pasello, Alessandra De Feo, Cristian Bassi, Clara Guerzoni, Lorena Landuzzi, Pier-Luigi Lollini, Davide Maria Donati, Massimo Negrini, Mauro Magnani, Katia Scotlandi
Summary: The modulation of CD99 in Ewing sarcoma may alter transcriptional dysregulation and affect the development of the tumor by controlling the zyxin-GLI1 axis.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Ivan Vannini, Manuela Ferracin, Francesco Fabbri, Muller Fabbri
Summary: The expression of non-coding RNAs is dysregulated in human cancers, particularly the transcribed ultraconserved regions (T-UCRs). In lung cancer, uc.83-, an antisense ultraconserved element, is upregulated and plays a role in cell growth. AKT and ERK 1/2 signaling pathways are involved in the oncogenic function of uc.83-. Inhibition of uc.83- could be a potential therapeutic treatment for non-small cell lung cancer.
Editorial Material
Oncology
Alessandra Care, Donatella Del Bufalo, Antonio Facchiano
Article
Biology
Beatrice Maranini, Giovanni Ciancio, Manuela Ferracin, Rosario Cultrera, Massimo Negrini, Silvia Sabbioni, Marcello Govoni
Summary: In this review, the role of ncRNAs in the immune system crosstalk during SARS-CoV-2 infection, specifically focusing on inflammatory pathways and cytokine storm syndromes, is discussed. The study aims to investigate the interaction between SARS-CoV-2 and the host, exploring the potential of miRNA-based therapeutics in tackling SARS-CoV-2 complications.
Article
Endocrinology & Metabolism
Camilla Cristalli, Maria Cristina Manara, Sergio Valente, Evelin Pellegrini, Alberto Bavelloni, Alessandra De Feo, William Blalock, Elisabetta Di Bello, David Pineyro, Angelika Merkel, Manel Esteller, Oscar M. Tirado, Antonello Mai, Katia Scotlandi
Summary: This study found that the novel nonnucleoside DNMT inhibitor MC3343 can specifically deplete DNMT1 in Ewing sarcoma through a mechanism independent of DNA methylation, leading to perturbations in the cell cycle and DNA damage. The depletion of DNMT1 activates p53-dependent signaling and apoptosis in p53wt cells, while in p53 mutated cells, it leads to persistent micronuclei and increased DNA instability. Treatment with MC3343 also enhances the efficacy of other DNA damaging agents, suggesting its potential as an adjuvant therapy for Ewing sarcoma.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Alessandra De Feo, Laura Pazzaglia, Lisa Ciuffarin, Fabio Mangiagli, Michela Pasello, Elisa Simonetti, Evelin Pellegrini, Cristina Ferrari, Giuseppe Bianchi, Benedetta Spazzoli, Katia Scotlandi
Summary: Ewing's sarcoma, a common pediatric bone tumor, relies on genetic and epigenetic alterations that induce gene expression reprogramming. The study identifies miR-214-3p as a common mediator of EWS-FLI1 and CD99, and its restoration inhibits tumor cell growth and migration by repressing HMGA1 expression.
Article
Pathology
Giorgio Durante, Giulia Veronesi, Cosimo Misciali, Mattia Riefolo, Martina Lambertini, Federico Tartari, Costantino Ricci, Manuela Ferracin, Emi Dika
Summary: The study found that dysplastic nevi (DN) and melanomas developed in the same patient have different small RNA expression profiles, supporting the hypothesis that DN are distinct biological entities rather than melanoma precursors.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Oncology
Giorgia Castellani, Mariachiara Buccarelli, Valentina Lulli, Ramona Ilari, Gabriele De Luca, Francesca Pedini, Alessandra Boe, Nadia Felli, Mauro Biffoni, Emanuela Pilozzi, Giovanna Marziali, Lucia Ricci-Vitiani
Summary: MiR-378a-3p plays a critical role in CRC carcinogenesis as a tumor suppressor by establishing a finely tuned crosstalk with lncRNAs MALAT1 and NEAT1, restraining tumorigenic properties of CRC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Giulia Lombardi, Roberta Valeria Latorre, Alessandro Mosca, Diego Calvanese, Luisa Tomasello, Christian Boni, Manuela Ferracin, Massimo Negrini, Nader Al Dewik, Mohamed Yassin, Mohamed A. Ismail, Bruno Carpentieri, Claudio Sorio, Paola Lecca
Summary: This study analyzed the modulation of the K562 transcriptome in the context of Chronic Myeloid Leukemia (CML) using the tumor suppressor gene PTPRG and the tyrosine kinase inhibitor Imatinib. The analysis identified genes whose expression levels were altered by PTPRG and Imatinib, providing insights into the transcriptomic landscape of CML. Two different methods were used in the analysis, with one focusing on gene set enrichment analysis and the identification of transcription factors, and the other focusing on selecting transcription factors from differentially expressed genes. The study revealed the potential upregulation of RARG and CD36 genes by PTPRG, suggesting a role in cell differentiation. The findings provide new insights into the molecular mechanisms of tumor suppression in the context of CML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Sara Vaccaro, Alessandra Rossetti, Antonella Porrazzo, Simona Camero, Matteo Cassandri, Silvia Pomella, Miriam Tomaciello, Giampiero Macioce, Francesca Pedini, Giovanni Barillari, Cinzia Marchese, Rossella Rota, Giovanni Cenci, Mario Tombolini, Robert A. Newman, Peiying Yang, Silvia Codenotti, Alessandro Fanzani, Francesca Megiorni, Claudio Festuccia, Giuseppe Minniti, Giovanni Luca Gravina, Francesca Vulcano, Luisa Milazzo, Francesco Marampon
Summary: The therapeutic properties of PBI-05204, an extract from Nerium oleander, were studied in the treatment of rhabdomyosarcoma (RMS). PBI-05204 inhibited the growth and invasion abilities of RMS and improved its sensitivity to radiotherapy. Additionally, PBI-05204 suppressed the formation of cancer stem cells in RMS.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Elisabetta Broseghini, Daria Maria Filippini, Laura Fabbri, Roberta Leonardi, Andi Abeshi, Davide Dal Molin, Matteo Fermi, Manuela Ferracin, Ignacio Javier Fernandez
Summary: Laryngeal squamous cell cancer (LSCC) is a common malignant tumor in the head and neck region with a poor prognosis. Tobacco smoking and alcohol abuse are the main risk factors. Identifying effective diagnostic and prognostic biomarkers, such as microRNAs (miRNAs), for LSCC is crucial for disease management and improving clinical outcomes. This systematic review provides an overview of tissue and circulating miRNAs and their potential role in diagnosing and predicting LSCC.
Review
Medicine, General & Internal
Daria Maria Filippini, Elisabetta Broseghini, Francesca Carosi, Davide Dal Molin, Mattia Riefolo, Laura Fabbri, Andi Abeshi, Ignacio Javier Fernandez, Manuela Ferracin
Summary: Head and neck cancer of unknown primary (HNCUP) refers to cervical lymph node metastases without an identifiable primary tumor. The management of HNCUP patients is challenging due to controversial guidelines in diagnosis and treatment. This systematic review presents available data on diagnostic and prognostic molecular biomarkers for HNCUP, with HPV and EBV being the only biomarkers currently used in clinical practice. Improved molecular profiling and development of tissue-of-origin classifiers are necessary for better diagnosis, staging, and therapeutic management of HNCUP patients.
Article
Cell Biology
Valeria Coppola, Ilaria Marino, Uwe Warnken, Mario Falchi, Luca Pasquini, Mauro Biffoni, Ruggero De Maria, Tobias Longin Haas
Summary: We have identified FYCO1 as a protein that promotes the transport of autophagic and endosomal vesicles. It interacts with activated CASP8 and its loss leads to increased sensitivity of cells to apoptosis and impaired transport of TNFRSF10B/TRAIL-R2/DR5.
Article
Biochemistry & Molecular Biology
Stefano Miglietta, Giulia Girolimetti, Lorena Marchio, Manuela Sollazzo, Noemi Laprovitera, Sara Coluccelli, Dario De Biase, Antonio De Leo, Donatella Santini, Ivana Kurelac, Luisa Iommarini, Anna Ghelli, Davide Campana, Manuela Ferracin, Anna Myriam Perrone, Giuseppe Gasparre, Anna Maria Porcelli
Summary: This article analyzes a case of SCCOPT and provides in-depth characterization of its features and characteristics. SCCOPT is a rare and aggressive tumor with poor prognosis, and its biology and molecular features have not been thoroughly investigated yet. The analysis of miRNA expression profile and metabolic markers can provide useful information for future diagnoses.