Article
Immunology
Johnathan Canton, Hanna Blees, Conor M. Henry, Michael D. Buck, Oliver Schulz, Neil C. Rogers, Eleanor Childs, Santiago Zelenay, Hefin Rhys, Marie-Charlotte Domart, Lucy Collinson, Andres Alloatti, Cara J. Ellison, Sebastian Amigorena, Venizelos Papayannopoulos, David C. Thomas, Felix Randow, Caetano Reis e Sousa
Summary: DNGR-1 is a dedicated cross-presentation receptor that promotes phagosomal rupture, allowing corpse-associated antigens to access the major histocompatibility complex class I antigen processing pathway. This mechanism reveals the existence of innate immune receptors that couple ligand binding and endocytic vesicle damage to regulate adaptive immunity.
Article
Biochemistry & Molecular Biology
Mikihiro Yoshie, Kazuya Kusama, Risaka Tanaka, Takanori Okubo, Junya Kojima, Yotaro Takaesu, Keiichi Isaka, Hirotaka Nishi, Kazuhiro Tamura
Summary: Previous in vitro studies have suggested that CALR may be associated with decidualization, and using pregnant rat models, it was found that uterine CALR expression was enhanced during implantation and decidualization. In humans, endometrial CALR expression tended to increase after polypectomy, suggesting a cooperative expression of endometrial factors that could enhance endometrial receptivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Marine Gros, Elodie Segura, Derek C. Rookhuizen, Blandine Baudon, Sandrine Heurtebise-Chretien, Nina Burgdorf, Mathieu Maurin, Eugene A. Kapp, Richard J. Simpson, Patrycja Kozik, Jose A. Villadangos, Mathieu J. M. Bertrand, Marianne Burbage, Sebastian Amigorena
Summary: Despite its importance in immune responses, the molecular pathways underlying antigen cross-presentation are not fully understood. This study reveals that membrane repair plays a crucial role in containing antigen export to the cytosol and cross-presentation in conventional dendritic cells (cDCs).
Article
Oncology
Tim B. Fessenden, Lauren E. Stopfer, Fiona Chatterjee, Julian Zulueta, Josh Mesfin, Therese Cordero Dumit, Irene Reijers, Esmee P. Hoefsmit, Christian Blank, Forest White, Stefani Spranger
Summary: Cytotoxic CD8(+) T cells must recognize tumor-derived antigens to achieve effective tumor elimination. Our study shows that dendritic cells induce cytotoxic CD8(+) T-cell responses by cross-presenting tumor-derived peptides, and the proportion of membrane-derived neoantigens is associated with reduced survival and treatment response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Immunology
Kristel Joy Yee Mon, J. Magarian Blander
Summary: Viral blockade of TAP affects MHC-I levels in DCs and impairs CD8 T-cell immunity. However, noncanonical cross-presentation in DCs can prime TAP-independent CD8 T cells that are better matched against the infection. Exploiting noncanonical cross-presentation can prevent chronic infections and control immune-evasive cancers.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Takuro Matsuoka, Akira Hattori, Shinya Oishi, Mitsugu Araki, Biao Ma, Toshiki Fujii, Norihito Arichi, Yasushi Okuno, Hideaki Kakeya, Sho Yamasaki, Hiroaki Ohno, Shinsuke Inuki
Summary: This study established an MR1 presentation reporter assay system using split-luciferase, which can efficiently explore MR1 ligands. Phenylpropanoid derivatives, including coniferyl aldehyde, were identified as MR1 ligands that can inhibit the MR1-MAIT cell axis. Further investigation revealed the key structural features required for MR1 recognition. These results have significant implications for identifying a wide range of MR1 ligands and developing novel MAIT cell modulators.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Takuro Matsuoka, Akira Hattori, Shinya Oishi, Mitsugu Araki, Biao Ma, Toshiki Fujii, Norihito Arichi, Yasushi Okuno, Hideaki Kakeya, Sho Yamasaki, Hiroaki Ohno, Shinsuke Inuki
Summary: In this study, a split-luciferase assay system was established to identify MR1 ligands, including phenylpropanoid derivatives. The structure-activity relationship analysis of coniferyl aldehyde analogs revealed the key features required for MR1 recognition. These findings will contribute to the identification of a wide range of endogenous and exogenous MR1 ligands and the development of novel MAIT cell modulators.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Annika Nelde, Jonas Rieth, Malte Roerden, Marissa L. Dubbelaar, Naomi Hoenisch Gravel, Jens Bauer, Reinhild Klein, Tobias Hoheisel, Hartmut Mahrhofer, Siri Goepel, Michael Bitzer, Sebastian Hoerber, Andreas Peter, Jonas S. Heitmann, Juliane S. Walz
Summary: Levels of soluble HLA molecules (sHLA) are significantly increased in COVID-19 patients and convalescent individuals, positively correlating with SARS-CoV-2-directed cellular immunity. Severe COVID-19 cases show reduced sHLA levels. The soluble immunopeptidome in COVID-19 patients exhibits increased diversity of HLA-presented peptides, and a SARS-CoV-2-derived peptide from the viral nucleoprotein is identified. Levels of sHLA directly correlate with the diversity of the soluble immunopeptidome.
Review
Chemistry, Multidisciplinary
Jeffrey Y. W. Mak, Ligong Liu, David P. Fairlie
Summary: Researchers have made significant contributions in the field of microbial natural products and synthetic ligands over the past decade, particularly focusing on compounds related to riboflavin and uracils that modulate immune cells. Through collaboration, they discovered a naturally occurring compound synthesized by bacteria that activates MAIT cells, leading to its use in detecting and characterizing MAIT cells in tissues.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Zhaofei Guo, Yining Zhu, Guangsheng Du, Ming Qin, Chunting He, Penghui He, Yuanshuai Song, Wenfei Chen, Shuting Bai, Fuhua Wu, Nan Qiao, Min Jiang, Xianjin Luo, Yuandong Zhang, Tao Gong, Zhirong Zhang, Xun Sun
Summary: This study reports a facile strategy for the rapid development of a highly effective cross-protective Pseudomonas aeruginosa vaccine. By loading cytosolic antigens derived from bacterial lysates into mesoporous silica nanospheres and fusing membrane antigens onto the surface, the engineered nano-vaccines induced potent humoral and cellular immune responses and protected against different strains of Pseudomonas aeruginosa infection.
Review
Immunology
Conor M. Henry, Carlos A. Castellanos, Caetano Reis E. Sousa
Summary: In this review, the role of dendritic cell natural killer group receptor-1 (DNGR-1) in cDC1 and its functions in anti-viral and anti-tumor immunity are discussed. The study found that DNGR-1 can promote inducible rupture of phagocytic or endocytic compartments containing dead cell debris, making dead cell-associated antigens accessible to cDC1. Additionally, DNGR-1 can recognize dead cells and participate in immune response. Therefore, the study of DNGR-1 has opened new perspectives into cross-presentation, which may have applications in immunotherapy of cancer and vaccination against viral diseases.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Immunology
Nicholas J. Shields, Estelle M. Peyroux, Katrin Campbell, Sunali Mehta, Adele G. Woolley, Claudio Counoupas, Silke Neumann, Sarah L. Young
Summary: Proteases released from dying cells can degrade antigens and generate proteolytic intermediates that enhance immune cell responses.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Chemistry, Analytical
Mihaela Puiu, Cristina Nativi, Camelia Bala
Summary: The development of sensitive and selective platforms for cancer biomarker detection is crucial for early diagnosis, disease monitoring, and treatment. This review provides an introduction to tumour-associated antigens (TAAs) and their application in recognizing different types and stages of cancer. The review also discusses the use of electrochemical bio/immunosensors designed for TAA detection in point-of-care (POC) environments, with a focus on the detection of small fragments of TAAs and the feasibility of organic-inorganic hetero-nano-interfaces-based bio/immunosensors for POC applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Sofia Khazan-Kost, Gal Cafri, Dganit Melamed Kadosh, Navit Mooshayef, Sumit Chatterji, Dan Dominissini, Sigal Manor, Bracha Zisser, Limor Broday, Efrosiniia Talalai, Anat Shemer, Oranit Zadok, Efrat Ofek, Amir Onn, Arie Admon, Michael Peled
Summary: sHLA-peptide complexes in pleural effusions can serve as a source of biomarkers for malignant tumors and potential candidates for personalized immunotherapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biology
Gerald Willimsky, Christin Beier, Lena Immisch, George Papafotiou, Vivian Scheuplein, Andrean Goede, Hermann-Georg Holzhuetter, Thomas Blankenstein, Peter M. Kloetzel
Summary: Proteasome-catalyzed peptide splicing (PCPS) can generate attractive neoepitopes for T cell receptor (TCR)-based adoptive T cell therapy, but in vivo experiments failed to detect any specific T cell response to these neo-splicetopes and provided no evidence that they were naturally processed and presented. Additionally, only one of the predicted neo-splicetopes was generated in vitro by PCPS, raising questions about the reliability of algorithms and in vitro reactions for simulating in vivo splicing.