Article
Biochemistry & Molecular Biology
Yan Huang, Han Zhao, Yuting Zhang, Yulong Tang, Xiangguang Shi, Shuai Jiang, Weilin Pu, Jing Liu, Yanyun Ma, Juiming Lin, Jinran Lin, Wenyu Wu, Yiyi Gong, Jiucun Wang, Qingmei Liu
Summary: By reanalyzing skin RNA-sequencing data, we found that the focal adhesion pathway was up-regulated and Zyxin appeared to be the primary focal adhesion protein involved in skin fibrosis. Furthermore, we verified its expression in Chinese skin tissues of several fibrotic diseases and found that Zyxin inhibition could significantly alleviate skin fibrosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Environmental Sciences
Mei Ha, Xu Huang, Lianbing Li, Daru Lu, Changjiang Liu
Summary: The study found that cypermethrin (CYP) can stimulate thyroid hormone (TH) biosynthesis and disrupt thyroid function. Protein kinase C alpha (PKC alpha) plays a crucial role in CYP-induced hyperthyroidism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Gwen R. Buel, Huy Q. Dang, John M. Asara, John Blenis, Anders P. Mutvei
Summary: This study found that prolonged deprivation of arginine and/or leucine leads to reactivation of mTORC1 activity, reaching activation levels similar to those observed in nutrient-rich conditions. Surprisingly, this reactivation is independent of amino acid regeneration, but dependent on PI3K/Akt signaling. These findings expand our understanding of the role of mTORC1 in growth-related diseases and suggest that dietary intervention by removing leucine and/or arginine may be an ineffective therapeutic approach.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Zuping Wu, Chenchen Zhou, Quan Yuan, Demao Zhang, Jing Xie, Shujuan Zou
Summary: CTGF promotes intercellular communication in chondrocytes through upregulating Cx43 expression and activating the PI3K/Akt signaling pathway.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Dongxiao Sun, Yali Hou, Yan Xie, Shaohua Yang, Bo Han, Lijun Shi, Xue Bai, Ruobing Liang, Tian Dong, Shengli Zhang, Qin Zhang
Summary: The study reveals the significant impact of EEF1D gene regulation on milk lipid synthesis in mammals, highlighting its importance in mammary gland development and dairy quality improvement.
Article
Biochemistry & Molecular Biology
Monika Mlinaric, Ivan Lucic, Lidija Milkovic, Ines V. da Silva, Ivana Tartaro Bujak, Vesna Musani, Graca Soveral, Ana Cipak Gasparovic
Summary: Aquaporin 3 (AQP3) is a membrane protein that transports hydrogen peroxide, water, and glycerol. It is implicated in tumor progression and malignancy in breast cancer and may be a target for therapy. This study examined the relationship between AQP3 expression, disruption of lipid raft harboring EGFR, and the EGFR/PI3K/Akt signaling pathway in breast cancer cell lines. The results indicate that AQP3 may not directly affect the PI3K/Akt pathway but rather its role is cell-line specific.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Fatemeh Vafashoar, Kazem Mousavizadeh, Hadi Poormoghim, Amir Haghighi, Salar Pashangzadeh, Nazanin Mojtabavi
Summary: The study found that progesterone treatment increased collagen content in fibrotic and normal lung tissues, as well as increased alpha-SMA and TGF-beta in fibrotic lung tissues while decreasing MMP9. Furthermore, progesterone treatment also decreased the gene expression of Col1a2, Ctgf, and End1 in bleomycin-injured lung tissues.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Irene Rosa, Eloisa Romano, Bianca Saveria Fioretto, Daniele Guasti, Lidia Ibba-Manneschi, Marco Matucci-Cerinic, Mirko Manetti
Summary: Research indicates that in a mouse model of bleomycin-induced scleroderma, the dermal network of Telocytes (TCs)/CD34(+) stromal cells is impaired, with most cells undergoing degeneration and loss. The findings suggest that this mouse model closely mimics the pathological mechanisms underlying TC damage and the contribution of TC loss to dermal fibrosis development/progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ming-Li Zou, Ying-Ying Teng, Zhong-hua Chen, Si-Yu Liu, Yuan Jia, Kai-Wen Zhang, Jun-Jie Wu, Zheng-Dong Yuan, Xiao-Yu Tang, Shun Yu, Jun-Xing Ye, Xia Li, Xiao-Jin Zhou, Feng-Lai Yuan
Summary: Skin fibrosis is a common pathological feature of various diseases. The urokinase-type plasminogen activator (uPA) system, including uPA, urokinase plasminogen activator receptor (uPAR), and plasminogen activator inhibitor-1 (PAI-1), is upregulated in fibrotic diseases. The successful binding of uPA and uPAR activates the downstream peroxisome proliferator-activated receptor (PPAR) signaling pathway, reducing the proliferation, migration, and contraction of disease-derived fibroblasts and alleviating skin fibrosis. However, increased or robust upregulation of PAI-1 inhibits these effects, suggesting the involvement of PAI-1 in skin fibrosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Naoko Takamura, Ludivine Renaud, Willian Abraham da Silveira, Carol Feghali-Bostwick
Summary: Systemic sclerosis is an autoimmune disease characterized by vasculopathy and excessive fibrosis, with limited treatment options available. Research suggests that abnormal regulation of MCHR1 may play a role in the pathogenesis of SSc, and inhibiting MCHR1 could be a novel therapeutic strategy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Han Dai, Wenjing Hu, Lianying Zhang, Feiyu Jiang, Xiongmin Mao, Gangyi Yang, Ling Li
Summary: Research showed that FGF21 was significantly downregulated in prostate cancer tissues and cell lines, and inhibited PCa cell proliferation and promoted apoptosis through facilitated autophagy.
CELL DEATH & DISEASE
(2021)
Review
Oncology
Yubo Xiang, Yingxin Yang, Jia Liu, Xu Yang
Summary: Osteosarcoma is a primary malignant bone tumor that mainly affects children and adolescents, and it is often associated with overactivation of the PI3K/AKT pathway. MicroRNAs (miRNAs) are small RNA molecules that play a role in gene regulation, and they are involved in the PI3K/AKT pathway and the development of osteosarcoma. MiRNAs can influence various cellular functions by targeting the PI3K/AKT pathway and regulating the expression of osteosarcoma-related genes. Additionally, miRNAs associated with the PI3K/AKT pathway have potential as biomarkers for osteosarcoma diagnosis, treatment, and prognosis assessment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chuan-Hua Zhan, Dong-Shen Ding, Wei Zhang, Hong-Liang Wang, Zhe-Yu Mao, Guo-Jun Liu
Summary: This study found that AKAP3 is overexpressed in breast cancer and promotes cell proliferation, invasion, and migration by activating the PTEN/PI3K/AKT/mTOR signaling pathway. Breast cancer patients with high AKAP3 expression have a worse prognosis, suggesting that it may serve as a prognostic indicator for breast cancer survival.
Review
Biochemistry & Molecular Biology
Devis Benfaremo, Silvia Agarbati, Matteo Mozzicafreddo, Chiara Paolini, Silvia Svegliati, Gianluca Moroncini
Summary: Systemic sclerosis, also known as scleroderma or SSc, is a highly varied disease in terms of clinical presentation, disease progression, and treatment response. Recent advancements in skin molecular profiling technologies have enabled the identification of patient subgroups based on gene expression analysis. This narrative review provides an overview of the current status of skin gene expression analysis and emphasizes the benefits of stratifying patients based on their gene signatures. This stratification has the potential to lead to a precision medicine approach in the management of SSc.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Chaojian Xu, Ziyue Wang, Long Zhang, Yi Feng, Jia Lv, Zhuangzhuang Wu, Rong Yang, Taiyong Wu, Jian Li, Ruhao Zhou, Zhi Tian, Junjun Bai, Huadong Zhang, Yanping Lan, Zhi Lv
Summary: This study found that POSTN is highly expressed in osteosarcoma and is closely related to poor prognosis. Transfection of a POSTN-shRNA plasmid effectively inhibits the proliferation, invasion, and migration of osteosarcoma cells, indicating that POSTN may promote the progression and metastasis of osteosarcoma by activating the PI3K/Akt signaling pathway.
CANCER CELL INTERNATIONAL
(2022)
Article
Immunology
Hideki Nakajima, Kimiko Nakajima, Mikiro Takaishi, Kentaro Ohko, Satoshi Serada, Minoru Fujimoto, Tetsuji Naka, Shigetoshi Sano
Summary: LRG, an acute phase protein mainly produced by the liver, has been recognized as an inflammatory biomarker for rheumatoid arthritis, inflammatory bowel diseases, and psoriasis. Studies have shown that LRG not only correlates well with disease activity in psoriasis patients, but also modulates the cytokine loops involved in psoriasiform inflammation.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Rheumatology
Hideki Nakajima, Kimiko Nakajima, Satoshi Serada, Minoru Fujimoto, Tetsuji Naka, Shigetoshi Sano
Summary: The study demonstrates that LRG promotes fibrotic processes in the SSc model through the TGF-β-Smad3 signaling pathway, suggesting LRG as a potential biomarker and therapeutic target for SSc in humans.
MODERN RHEUMATOLOGY
(2021)
Article
Oncology
Masahiro Koh, Tsuyoshi Takahashi, Yukinori Kurokawa, Teruyuki Kobayashi, Takuro Saito, Tomo Ishida, Satoshi Serada, Minoru Fujimoto, Tetsuji Naka, Noriko Wada, Kotaro Yamashita, Koji Tanaka, Yasuhiro Miyazaki, Tomoki Makino, Kiyokazu Nakajima, Makoto Yamasaki, Hidetoshi Eguchi, Yuichiro Doki
Summary: The study showed that propranolol inhibits tumor growth and induces cell cycle arrest and apoptosis in gastric cancer cell lines, suggesting its potential as a new drug for gastric cancer treatment.
Article
Oncology
Keiichiro Yokota, Satoshi Serada, Shigehiro Tsujii, Keisuke Toya, Tsuyoshi Takahashi, Takashi Matsunaga, Minoru Fujimoto, Sunao Uemura, Tsutomu Namikawa, Ichiro Murakami, Shogo Kobayashi, Hidetoshi Eguchi, Yuichiro Doki, Kazuhiro Hanazaki, Tetsuji Naka
Summary: Cholangiocarcinoma is a highly malignant cancer that often lacks effective therapy options, but the novel treatment option of GPC1-ADC targeting GPC1-positive cholangiocarcinoma cells shows promising anti-tumor effects through direct tumor growth inhibition and indirect inhibition of tumor angiogenesis. Additionally, GPC1 expression levels could serve as a prognostic indicator for patients with cholangiocarcinoma.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kie Nakatani, Satoshi Serada, Minoru Fujimoto, Kengo Obata, Tomoharu Ohkawara, Eri Sasabe, Tetsuya Yamamoto, Tetsuji Naka
Summary: The study revealed that SOCS1 gene therapy has anti-tumor effects on oral squamous cell carcinoma, inducing apoptosis through regulating the JAK/STAT-independent pathway. Overexpression of SOCS1 inhibited the JAK/STAT and p44/42 MAPK pathways, decreased Mcl-1 expression, and suppressed proliferation of OSCC cells.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2022)
Article
Oncology
Taihei Kajiyama, Satoshi Serada, Minoru Fujimoto, Tomoharu Ohkawara, Masahiro Komori, Masamitsu Hyodo, Tetsuji Naka
Summary: Overexpression of SOCS1 has a potential therapeutic effect on head and neck squamous cell carcinoma (HNSCC), inhibiting cell proliferation through cell cycle arrest and apoptosis. The overexpression of SOCS1 also inhibits the activity of phosphorylated STAT3 and p44/42 MAPK, as well as the expression of the anti-apoptotic protein Bcl-xL, leading to suppression of tumor growth. The study suggests the potential clinical application of SOCS1 gene therapy for HNSCC.
ANTICANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Tsukie Kin Tsukuda, Hiroshi Ohnishi, Minoru Fujimoto, Yu Nakatani, Kazufumi Takamatsu, Tetsuji Naka, Akihito Yokoyama
Summary: This study found that the proportion of Th2 cells in bronchoalveolar lavage fluid (BALF) was significantly higher in PF-ILD patients compared to non-PF-ILD patients. The increased proportion of Th2 cells in BALF was associated with greater deterioration of lung function in fibrotic interstitial lung diseases.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Mizuki Kanda, Satoshi Serada, Kosuke Hiramatsu, Masashi Funauchi, Kengo Obata, Satoshi Nakagawa, Tomoharu Ohkawara, Okinori Murata, Minoru Fujimoto, Fumiko Chiwaki, Hiroki Sasaki, Yutaka Ueda, Tadashi Kimura, Tetsuji Naka
Summary: In this study, an antibody-drug conjugate (ADC) targeting LSR was developed as a new therapeutic approach for epithelial ovarian cancer (EOC). The LSR-ADC demonstrated significant antitumor activity against LSR-positive EOC cell lines and tumors, suggesting its potential as a novel therapy for patients with LSR-positive ovarian cancer.
Article
Oncology
Yurina Saito, Tsuyoshi Takahashi, Kosuke Hiramatsu, Satoshi Serada, Minoru Fujimoto, Tomoharu Ohkawara, Takahito Sugase, Takahiko Nishigaki, Koji Tanaka, Yasuhiro Miyazaki, Tomoki Makino, Yukinori Kurokawa, Kiyokazu Nakajima, Makoto Yamasaki, Ken J. Ishii, Hidetoshi Eguchi, Yuichiro Doki, Tetsuji Naka
Summary: This study evaluated the potential synergistic antitumor activity of anti-GPC1 monoclonal antibody (mAb) and K3-SPG in GPC1-positive esophageal squamous cell carcinoma (ESCC). The combination therapy showed antitumor activity, possibly through the ADCC mechanism.
ANTICANCER RESEARCH
(2023)
Article
Oncology
Ryugo Teranishi, Tsuyoshi Takahashi, Yuuki Obata, Toshirou Nishida, Shuichi Ohkubo, Hiromi Kazuno, Yurina Saito, Satoshi Serada, Minoru Fujimoto, Yukinori Kurokawa, Takuro Saito, Kazuyoshi Yamamoto, Kotaro Yamashita, Koji Tanaka, Tomoki Makino, Kiyokazu Nakajima, Seiichi Hirota, Tetsuji Naka, Hidetoshi Eguchi, Yuichiro Doki
Summary: The combination of PIM and sunitinib was found to be effective against imatinib-resistant GIST by inhibiting KIT and angiogenic signaling pathways. It inhibited the growth and induced apoptosis of imatinib-resistant GIST cell lines in vitro, and enhanced antitumor responses in murine xenograft models compared to individual therapies. PIM also suppressed VEGF expression in GIST cells by targeting protein kinase D2 and hypoxia-inducible factor-1 alpha, which are both HSP90 client proteins.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Medicine, General & Internal
Natsuka Umezawa, Fumitaka Mizoguchi, Yasuhiro Maejima, Naoki Kimura, Hisanori Hasegawa, Tadashi Hosoya, Minoru Fujimoto, Hitoshi Kohsaka, Tetsuji Naka, Shinsuke Yasuda
Summary: This study investigated whether leucine-rich α-2 glycoprotein (LRG) could serve as a novel biomarker for large vessel vasculitides (LVV). The results showed that serum levels of LRG were higher in patients with active disease and decreased after treatment. However, LRG exhibited inferior performance as an indicator of disease activity compared to CRP and ESR.
FRONTIERS IN MEDICINE
(2023)
Article
Oncology
Masashi Funauchi, Satoshi Serada, Kosuke Hiramatsu, Eiji Funajima, Mizuki Kanda, Yoshikazu Nagase, Satoshi Nakagawa, Tomoharu Ohkawara, Minoru Fujimoto, Yuji Suzuki, Yutaka Ueda, Tadashi Kimura, Tetsuji Naka
Summary: Lipolysis-stimulated lipoprotein receptor (LSR) is a lipoprotein receptor associated with poor prognosis in cancer. LSR negatively regulates tumor immunity by repressing CD8+ T cells. Antibodies targeting LSR can inhibit tumor growth and enhance CD8+ T cell activity in tumor tissue.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Meeting Abstract
Oncology
Masashi Funauchi, Kosuke Hiramatsu, Satoshi Serada, Minoru Fujimoto, Tetsuji Naka