Article
Biochemical Research Methods
Zahed Khatooni, Navid Teymourian, Heather L. Wilson
Summary: This study introduces a novel strategy for epitope prediction using molecular dynamics simulation, homology modeling, and docking simulations. By selecting diverse SLA-1 alleles, the study successfully identifies virus epitopes that bind with high affinity to these alleles.
Article
Cell Biology
Shuai Lu, Xi-xiu Xie, Lei Zhao, Bin Wang, Jie Zhu, Ting-rui Yang, Guang-wen Yang, Mei Ji, Cui-ping Lv, Jian Xue, Er-hei Dai, Xi-ming Fu, Dong-qun Liu, Lun Zhang, Sheng-jie Hou, Xiao-lin Yu, Yu-ling Wang, Hui-xia Gao, Xue-han Shi, Chang-wen Ke, Bi-xia Ke, Chun-guo Jiang, Rui-tian Liu
Summary: The study reveals that predicted epitopes on SARS-CoV-2 effectively induce antibody production, with some being immunodominant. There are differences in immunodominant epitopes between individuals with domestic and imported SARS-CoV-2, possibly due to mutations on the proteins. Several epitopes on the S protein elicit neutralizing antibodies against different variants of SARS-CoV-2, showing potential for vaccine design against coronaviruses.
Article
Computer Science, Information Systems
Ayesha Afzal, Basit Shafiq, Shafay Shamail, Abeer Elahraf, Jaideep Vaidya, Nabil R. Adam
Summary: The paper proposes an approach for collaborative BP development that utilizes attribute and structural similarity as well as semantic information of operations to overcome syntactic and semantic heterogeneity among Web service operations across organizations. Experimental evaluation with real world data from e-commerce sales BPs demonstrates the effectiveness of the approach.
IEEE TRANSACTIONS ON SERVICES COMPUTING
(2021)
Article
Multidisciplinary Sciences
Carlos Wert-Carvajal, Ruben Sanchez-Garcia, Jose R. Macias, Rebeca Sanz-Pamplona, Almudena Mendez Perez, Ramon Alemany, Esteban Veiga, Carlos Oscar S. Sorzano, Arrate Munoz-Barrutia
Summary: The development of novel sequential methods and web server tools has improved the accuracy of T-cell epitope binding affinity predictions in mice, simplifying the prediction process of neoantigens.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Marcilio Jorge Fumagalli, Luiz Tadeu Moraes Figueiredo, Victor Hugo Aquino
Summary: This review summarizes 153 immunoreactive peptides from arboviruses of the Flavivirus genus, providing valuable data for those investigating the antibody response against flavivirus infection. The majority of peptides were described for DENV, possibly due to its long-lasting impact on human health and the lack of efficient vaccines and therapeutic methods. Immune landscape data regarding linear immunoreactive and continuous flavivirus peptides are still scarce, and a complete and more detailed map remains to be elucidated.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edlainne Pinheiro Ferreira-Sena, Daiana de Jesus Hardoim, Flavia de Oliveira Cardoso, Luiz Ney d'Escoffier, Isabela Ferreira Soares, Joao Pedro Rangel da Silva Carvalho, Ricardo Almir Angnes, Stenio Perdigao Fragoso, Carlos Roberto Alves, Salvatore Giovanni De-Simone, Josue da Costa Lima-Junior, Alvaro Luiz Bertho, Tania Zaverucha-do-Valle, Franklin Souza da Silva, Katia da Silva Calabrese
Summary: Leishmaniasis is a complex and diverse disease that is considered a major public health problem. Despite the availability of treatment options, there are currently no vaccines for cutaneous leishmaniasis. This study focused on the prediction and characterization of antigenic epitopes that could interact with major histocompatibility complex class I in mice or humans. Through a combination of immunogenicity prediction and interaction assays, nine antigenic peptides were identified as strong candidates for the development of a peptide vaccine against leishmaniasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Salvatore Giovanni De-Simone, Larissa Rodrigues Gomes, Paloma Napoleao-Pego, Guilherme Curty Lechuga, Jorge Soares de Pina, Flavio Rocha da Silva
Summary: The study identified 20 B-cell epitopes on the diphtheria toxin protein, distributed across different domains. Chemically synthesized biepitope peptides showed high sensitivity and specificity in ELISA for diagnosis of diphtheria, demonstrating potential for developing new diagnostic assays.
Article
Chemistry, Analytical
Yuchen Bai, Jie Fei, Weilin Wu, Leina Dou, Minggang Liu, Shibei Shao, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: This study determined the minimum distance between two epitopes in sandwich immunoassays for small molecules. The results provide a theoretical basis for hapten design, antibody discovery and development, and the establishment of sandwich immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Rhiane Moody, Kirsty Wilson, Nirmala Chandralega Kampan, Orla M. McNally, Thomas W. Jobling, Anthony Jaworowski, Andrew N. Stephens, Magdalena Plebanski
Summary: Autoantibodies recognizing phosphorylated heat shock factor 1 (HSF1-PO4) are proposed as potential new diagnostic biomarkers for early-stage high-grade serous ovarian cancer (HGSOC). Specific regions within HSF1 were identified, confirmed as useful biomarkers with comparable diagnostic potential to the full protein. Antibody responses to HSF1-PO4 and corresponding peptides increased post chemotherapy, suggesting these short peptide sequences could support early diagnosis or monitor responses to treatment.
Article
Immunology
Alexandra Sanchez-Martinez, Liliana Acevedo-Saenz, Juan Carlos Alzate-Angel, Cristian M. Alvarez, Fanny Guzman, Tanya Roman, Silvio Urcuqui-Inchima, Walter D. Cardona-Maya, Paula Andrea Velilla
Summary: CD8(+) T-cells are important in controlling HIV replication. Certain mutations in HLA-I interaction with viral peptides play a significant role in the adaptation of circulating strains of HIV-1. This study examined the functional response of CD8(+) T-cells to mutated epitopes in individuals with HIV-1. The results suggest that the binding affinity between mutated peptides and HLA molecules may be associated with the quality of the immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Marta Pacheco, Beatriz Jurado-Sanchez, Alberto Escarpa
Summary: In this study, Janus micromotors encapsulating transition metal dichalcogenides (TMDs) and modified with a rhodamine-labeled affinity peptide were successfully used for Salmonella enterica endotoxin detection. The OFF-ON strategy based on the specific binding of the peptide with the TMDs allowed for fluorescence quenching and subsequent recovery upon binding to the endotoxin. The developed method demonstrated high sensitivity, selectivity, and bacterial specificity, showing potential for multiplexed assays and portable detection devices.
Article
Green & Sustainable Science & Technology
Phil Brown, Brian Baldassarre, Jan Konietzko, Nancy Bocken, Ruud Balkenende
Summary: Circular oriented innovation aims to solve sustainability problems by redesigning industrial products, processes, business models, and value networks. Collaboration is crucial but few tools exist to support it. A new tool integrating effectuation and design thinking principles was developed and tested, showing high user receptivity and the importance of expert facilitation for maintaining focus on circularity.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Sara Feola, Markus Haapala, Karita Peltonen, Cristian Capasso, Beatriz Martins, Gabriella Antignani, Antonio Federico, Vilja Pietiainen, Jacopo Chiaro, Michaela Feodoroff, Salvatore Russo, Antti Rannikko, Manlio Fusciello, Satu Koskela, Jukka Partanen, Firas Hamdan, Sari M. Tahka, Erkko Ylosmaki, Dario Greco, Mikaela Gronholm, Tuija Kekarainen, Masoumeh Eshaghi, Olga L. Gurvich, Seppo Yla-Herttuala, Rui M. M. Branca, Janne Lehtio, Tiina M. Sikanen, Vincenzo Cerullo
Summary: This study successfully identified and characterized tumor-specific ligands using a microfluidic-based chip, highlighting the potential for personalized cancer therapeutic vaccine design. The developed technology showed improved efficiency in isolating peptides and has the capability for high-throughput analysis with potential clinical applications.
Editorial Material
Cell Biology
Paulina Strzyz
Summary: The researchers have developed a straightforward, cell-free assay to study the functions of microtubule-associated proteins.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Eric Savier, Lorena Simon-Gracia, Frederic Charlotte, Pierre Tuffery, Tambet Teesalu, Olivier Scatton, Angelita Rebollo
Summary: The study demonstrated that bi-functional tumor-penetrating and interfering peptides are internalized in malignant hepatocytes, inducing apoptosis, while having no effect on non-malignant cells. The degree of peptide internalization correlated with receptor expression levels and tumor aggressiveness.