Article
Environmental Sciences
Jagadish Chandra Kumar Mangu, Marios Stylianou, Per-Erik Olsson, Jana Jass
Summary: The exposure to PFAS increases susceptibility to pathogens in Caenorhabditis elegans by reducing host immunity and increasing intestinal membrane permeability, while also increasing bacterial virulence, with potential implications for humans and other animals.
ENVIRONMENTAL POLLUTION
(2022)
Article
Fisheries
Qiu-Ning Liu, Ying-Yu Tang, Si-Pei Zhang, Yue-Tian Li, Gang Wang, Dai-Zhen Zhang, Sen-Hao Jiang, Hui Yang, Bo-Ping Tang, Li-Shang Dai
Summary: Using transcriptome analysis, this study investigated the immunological response of Procambarus clarkii to Vibrio cholerae, identifying several significantly altered gene expressions related to antioxidants, phagocytosis, and pathogen clearance. The study also confirmed the up-regulation of immune defense genes through quantitative real time PCR, providing insights for potential defense strategies against Vibrio cholerae infection in P. clarkii.
Article
Immunology
Dustin A. Farr, Dhrubajyoti Nag, Jeffrey H. Withey
Summary: The study reveals that zebrafish can serve as a natural host model for Vibrio cholerae, exhibiting similar diarrheal symptoms as humans. By assessing various immunological markers, it was found that infection with V. cholerae in zebrafish leads to increased gene expression of T-bet and GATA3, as well as elevated levels of mucosal antibodies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Ying-Xiu Li, Nan-Nan Wang, Yan-Xia Zhou, Chun-Guo Lin, Jing-Shan Wu, Xin-Qi Chen, Guan-Jun Chen, Zong-Jun Du
Summary: Marine probiotics show potential as a substitute for antibiotics in aquaculture to combat drug-resistant pathogens. The potential marine probiotic ML1206 was found to significantly inhibit Vibrio anguillarum colonization in the intestinal tract of Caenorhabditis elegans, with strong antibacterial capabilities and great acid and bile salt tolerance. This study provides a new direction for screening marine probiotics and supports the potential application of ML1206 in aquaculture.
Article
Immunology
Yaovi M. G. Hounmanou, Elisabeth Njamkepo, Jean Rauzier, Karin Gallandat, Aurelie Jeandron, Guyguy Kamwiziku, Klaudia Porten, Francisco Luquero, Aaron Aruna Abedi, Baron Bashige Rumedeka, Berthe Miwanda, Martin Michael, Placide Welo Okitayemba, Jaime Mufitini Saidi, Renaud Piarroux, Francois-Xavier Weill, Anders Dalsgaard, Marie-Laure Quilici
Summary: Lake Tanganyika basin in Africa is a hotspot for cholera. Vibrio cholerae O1 isolates collected from the Democratic Republic of the Congo side of the lake during 2001-2020 belonged to 2 out of the 5 clades of the AFR10 sublineage. One clade became dominant after acquiring a parC mutation that reduced susceptibility to ciprofloxacin.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Gastroenterology & Hepatology
Guangli Wang, Chan Fan, Hui Wang, Chengyi Jia, Xiaoting Li, Jianru Yang, Tao Zhang, Song Gao, Xun Min, Jian Huang
Summary: This study reveals a novel regulatory role of the FHA domain protein TagH in controlling the hemolytic activity of Vibrio cholerae, in addition to regulating the T6SS. TagH negatively regulates HlyA expression at the transcriptional and post-translational levels, and the phosphopeptide binding sites of the FHA domain play a crucial role in the regulation of hemolytic activity. Deletion of tagH enhances the intestinal pathogenicity and extraintestinal invasion ability of V. cholerae, which mostly rely on the expression of HlyA.
Editorial Material
Biochemistry & Molecular Biology
Jeremy J. Grubbs, David M. Raizen
Summary: Fatigue and sleepiness are common responses to tissue injury, and a new study shows how the innate immune system mediates injury-induced sleep.
Article
Biochemistry & Molecular Biology
Junxiang Yan, Qian Liu, Xinke Xue, Jinghao Li, Yuehua Li, Yingying Su, Boyang Cao
Summary: This study reveals that the VC1795 gene plays a key role in the intestinal colonization and pathogenicity of Vibrio cholerae, and it regulates the expression of its downstream gene, VC1794, and the toxin-coregulated pilus (TCP) cluster. These findings provide insights into the regulatory mechanisms of VC1795 in bacterial pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
John C. Macbeth, Rui Liu, Salma Alavi, Ansel Hsiao
Summary: The composition of the gut microbiome at the time of infection or vaccination appears to be crucial for providing robust mucosal immunity against Vibrio cholerae. In cholera-endemic areas, dysbiotic human gut microbiomes may suppress the immune response against V. cholerae via CD4+ lymphocytes.
Article
Microbiology
Vishal Mevada, Rajesh Patel, Pravin Dudhagara, Rajesh Chaudhari, Mustafa Vohra, Vikram Khan, Douglas J. H. Shyu, Yih-Yuan Chen, Dolatsinh Zala
Summary: This study aims to elucidate the heterogeneity of the sporadic clinical strain of V. cholerae VC01 among patients residing in Silvasa. The investigation demonstrated the existence of various genetic alterations and resistance profiles against multiple drugs. The present study helps to establish a correlation between the mutation and the coexistence of antimicrobial resistance toward current treatment.
Article
Microbiology
Murari Bhandari, Irani U. Rathnayake, Flavia Huygens, Son Nguyen, Brett Heron, Amy V. Jennison
Summary: This study characterized the genetic features of Vibrio cholerae O1 isolates from Australia and compared them with international isolates. The findings showed limited genetic relatedness between Australian and international strains, indicating different lineages. Understanding the origin of Australian strains and devising control measures are important for reducing Vibrio cholerae exposure.
MICROBIOLOGY SPECTRUM
(2023)
Article
Toxicology
Merle Marie Nicolai, Barbara Witt, Andrea Hartwig, Tanja Schwerdtle, Julia Bornhorst
Summary: The optimized AU assay for Caenorhabditis elegans serves as a new tool for genotoxicity testing, bridging the gap between in vitro and rodent experiments. Validation with known genotoxic agents confirms the method's meaningfulness, reproducibility, and feasibility for high-throughput genotoxicity testing, especially in preliminary screening.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Fisheries
XinHai Zhu, Shuangming Zhang, Liying Zhou, Shiqi Ao, Huanyu Tang, Yifan Zhou, Qiyun Chen, Xiaojian Gao, Qun Jiang, Xiaojun Zhang
Summary: This study aimed to explore the antimicrobial activity and immunomodulatory effect of Bacillus velezensis on non-O1 Vibrio cholerae infection in Macrobrachium nipponense. Results showed that treatment with B. velezensis led to significantly increased immune parameters and up-regulated expression of immune-related genes in M. nipponense. Additionally, the survival rate of M. nipponense challenged with non-O1 V. cholerae was higher in the B. velezensis treated group.
Article
Chemistry, Analytical
Anle Ge, Liang Hu, JiaXing Fan, Minghai Ge, Xixian Wang, Shanshan Wang, Xiaojun Feng, Wei Du, Bi-Feng Liu
Summary: The optogenetic method is commonly used to study neuronal function and connectivity in neural circuits. This study presents a portable optofluidic platform using optical fiber illumination for optogenetic manipulation in nematode C. elegans. It allows precise activation of neurons and simultaneous measurement of cellular signals, providing a simple, rapid, and cost-effective strategy for studying neural circuits.
Article
Microbiology
Yun Luo, Michael Payne, Sandeep Kaur, Sophie Octavia, Jianmin Jiang, Ruiting Lan
Summary: This study sequenced and compared non-pandemic Vibrio cholerae O1 isolates from Zhejiang, China with publicly available non-pandemic O1 genomes. The isolates were classified into three lineages, with one lineage sharing the most recent common ancestor with the pandemic clones. Each lineage had unique properties contributing to their disease-causing capacity. The findings provide new insights into the evolution of V. cholerae O1 for cholera prevention and control.
MICROBIOLOGY SPECTRUM
(2023)