期刊
AQUACULTURE
卷 547, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2021.737435
关键词
Immune response; Procambarus clarkii; Transcriptome; Vibrio cholerae
资金
- Open Funding Project of the Key Lab-oratory of Freshwater Aquatic Genetic Resources, Ministry of Agricul-ture and Rural Affairs [K2021-04]
- Natural Science Foundation of Jiangsu Province [BE2020673]
- Natural Science Foundation of Zhejiang Province [LQ20C190009]
- National Key R&D Program of China [2019YFD0900404-05]
- Industry-University-Research Coop-eration Project of Jiangsu Province [BY2020644]
- Open Funding Project of Anhui Province Key Laboratory of Aquaculture & Stock Enhancement [AHSC202001]
- 16th Six Talents Peak Project of Jiangsu Province [NY-126]
- National Natural Science Foundation of China [32070526]
- Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [201721]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX (18) 3027]
- Qing Lan Project of Jiangsu Province
- Outstanding Young Talents of YCTU
Using transcriptome analysis, this study investigated the immunological response of Procambarus clarkii to Vibrio cholerae, identifying several significantly altered gene expressions related to antioxidants, phagocytosis, and pathogen clearance. The study also confirmed the up-regulation of immune defense genes through quantitative real time PCR, providing insights for potential defense strategies against Vibrio cholerae infection in P. clarkii.
Procambarus clarkii is an important freshwater aquaculture species. One of the most serious diseases in P. clarkii is rotten shell disease. However, there is few report about the immune response of P. clarkii hepatopancreas to Vibrio cholerae. Using transcriptome analysis, this research investigated the immunological response of P. clarkii to V. cholerae at the gene expression level. It was found that 44,043,166 cleaning readings were present in the treatment group. A total of 34,237 unigenes were discovered, with a mean length of 1507. Moreover, 360 unigenes were found to have significantly altered expression, with 102 of them being considerably up-regulated. According to further annotation and analysis, it was found that DEGs are rich in Glutathione metabolism, Lysosome, Phagosome, Apoptosis, Adherens junction, and Leukocyte transient migration. These pathways are mostly associated with antioxidants, phagocytosis, and pathogen clearance. Furthermore, quantitative real timePCR (qRT-PCR) confirmed the up-regulation of immune defense genes of DEGs, which supported the RNA-seq data. This study may serve as a foundation for future research on the immunological response of P. clarkii, as well as provide recommendations for P. clarkii against V. cholerae infection.
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