Article
Biochemistry & Molecular Biology
Elena Rensen, Florian Mueller, Viviana Scoca, Jyotsana J. Parmar, Philippe Souque, Christophe Zimmer, Francesca Di Nunzio
Summary: Infected macrophages exhibit large nuclear foci of viral DNA and RNA, with clusters forming that do not require chromosomal integration and consist predominantly of incoming genomic RNA. This indicates a different understanding of the early HIV-1 replication cycle and potential implications for addressing viral persistence.
Review
Virology
Qi Shen, Chunxiang Wu, Christian Freniere, Therese N. Tripler, Yong Xiong
Summary: The protection of the HIV-1 capsid allows it to pass through the nuclear pore complexes into the nucleus, where reverse transcription occurs before uncoating and release of the viral genome near the integration site. These findings reveal a new paradigm in understanding the HIV-1 life cycle.
Article
Multidisciplinary Sciences
Chenglei Li, Ryan C. Burdick, Kunio Nagashima, Wei-Shau Hu, Vinay K. Pathak
Summary: The study showed that HIV-1 cores maintain their integrity until just before integration, losing a content marker approximately 1 to 3 minutes before uncoating. This suggests that viral cores retain their integrity and remain separated from the nuclear environment before uncoating occurs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Johanna Pispa, Elisa Mikkonen, Leena Arpalahti, Congyu Jin, Carmen Martinez-Fernandez, Julian Ceron, Carina I. Holmberg
Summary: The study suggests that AKIR-1 is an important regulator of the subcellular localization and activity of proteasomes in multicellular organisms, playing a crucial role in maintaining protein homeostasis.
Article
Microbiology
Hangil Kim, Hanako Shimura, Kae Sueda, Chikara Masuta
Summary: RNA silencing is an important defense mechanism of host plants against viral infection. Cucumber mosaic virus encodes a protein called 2b, which suppresses RNA silencing. This study found that the phosphorylation of 2b at position 28 negatively regulates its RNA silencing suppression activity by affecting its nuclear localization. The study also discovered that 2b's transport between the nucleus and cytoplasm is similar to that of Argonaute proteins, suggesting that 2b manipulates its nucleocytoplasmic trafficking to track down its targets in the RNA silencing pathway. The efficient RNA silencing suppression activity of 2b is maintained by a balance of phosphorylation and dephosphorylation and is coupled to importin/exportin-mediated shuttling between the nucleus and cytoplasm.
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuewen Luo, Xiantao Zhang, Ran Chen, Rong Li, Yang Liu, Junsong Zhang, Qin Liu, Meijun Si, Jun Liu, Bolin Wu, Xuemei Wang, Shijian Wu, Yiwen Zhang, Xu Zhang, Deyin Guo, Xin He, Ting Pan, Hui Zhang
Summary: This study found that the deubiquitinase USP10 can translocate into the nucleus and stabilize nuclear AID protein levels through AKT-mediated phosphorylation. Furthermore, the signals from BCR and TLR synergistically promote the phosphorylation of USP10. Deficiency of USP10 in B cells leads to decreased AID protein levels and reduced production of neutralizing antibodies.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Pharmacology & Pharmacy
Yanhui Song, Hongguang Zhang, Yinmiao Wang, Jin Guo, Shengjie Tang, Lu Wang, Ke Peng, Chun-sheng Dong
Summary: The study confirmed that KPNA2 is an important adaptor protein of the KPNB1-Pom121 pathway, mediating the nuclear transportation of HIV-1 pre-integration complex (PIC). This pathway may be a potential target for antiviral treatment against HIV-1.
ANTIVIRAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yan Wang, Chen Su, Yingjun Yu, Yuqing He, Hua Wei, Na Li, Hong Li, Jie Duan, Bin Li, Jigang Li, Seth J. Davis, Lei Wang
Summary: This study reveals that the clock regulator TIC controls the activity of the far-red light photoreceptor phyA in Arabidopsis, enhancing the plant's fitness under natural conditions.
Article
Microbiology
Helene Bauby, Christopher C. Ward, Rupert Hugh-White, Chad M. Swanson, Reiner Schulz, Caroline Goujon, Michael H. Malim
Summary: The interaction between HIV-1 and CD4(+) T cells have been studied through transcriptomics analysis, unveiling global changes in gene expression. The viral accessory protein Vpr plays a crucial role in these gene expression changes, highlighting its significance in infection and pathogenesis. This finding sheds new light on the interplay between HIV-1 and its host at the cellular level.
Article
Multidisciplinary Sciences
Mirjana Persaud, Anastasia Selyutina, Cindy Buffone, Silvana Opp, Daniel A. Donahue, Oliver Schwartz, Felipe Diaz-Griffero
Summary: The overexpression of human SUN2 and SUN1 proteins blocks HIV-1 infection in a capsid-dependent manner. These transmembrane proteins interact with the HIV-1 capsid in the nucleus, restricting viral replication. The ability of SUN1/2 to restrict HIV-1 correlates with their perinuclear localization and the interaction mediated by their N-terminal domains.
SCIENTIFIC REPORTS
(2021)
Review
Virology
Maryam Tabasi, Ivan Nombela, Julie Janssens, Adrien P. Lahousse, Frauke Christ, Zeger Debyser
Summary: Understanding the interactions between viral proteins and host proteins is crucial for studying the pathogenesis of HIV and developing antiviral therapies. The steps of HIV-1 uncoating and nuclear import remain highly debated, with controversies surrounding the viral and host factors involved in these processes. The role of transportin-SR2 as a host cell factor in active nuclear transport is comprehensively discussed in this review.
Article
Microbiology
Helena Fabryova, Sandra Kao, Sayaka Sukegawa, Eri Miyagi, Louis Taylor, Damien Ferhadian, Hideki Saito, Heiner Schaal, Frank Hillebrand, Klaus Strebel
Summary: Despite progress in drug therapies, HIV-1 remains incurable and can rebound due to drug resistance. Gelsolin (GSN) was identified as a novel Vpr-interacting protein and its expression was associated with HIV-1 infection in macrophages. Overexpression of GSN reduces the expression and packaging of HIV-1 envelope protein, leading to reduced viral infectivity.
Review
Oncology
Basia Galinski, Thomas B. Alexander, Daniel A. Mitchell, Hannah V. Chatwin, Chidiebere Awah, Adam L. Green, Daniel A. Weiser
Summary: Exportin-1 is a nuclear transport protein associated with inferior outcomes in cancer cells. Selinexor, an FDA-approved inhibitor of XPO1, has shown promising results in adult cancers but its efficacy in childhood cancers is still under investigation.
Article
Microbiology
Julie Janssens, Jolien Blokken, Yulia Lampi, Flore De Wit, Irena Zurnic Bonisch, Ivan Nombela, Paulien Van de Velde, Barbara Van Remoortel, Rik Gijsbers, Frauke Christ, Zeger Debyser
Summary: The study shows that TRN-SR2 directly mediates HIV-1 nuclear import via interaction with HIV-1 integrase, providing a potential target for rational drug design to develop new therapeutic strategies targeting the bottleneck of HIV-1 nuclear import.
MICROBIOLOGY SPECTRUM
(2021)
Article
Virology
Ippei Takeuchi, Tadahiro Nasukawa, Ryosuke Sugimoto, Iyo Takemura-Uchiyama, Hironobu Murakami, Jumpei Uchiyama
Summary: The study found that the propagation processes of phages need to be tailored according to the type of phage, and careful consideration should be given to the choice of methodologies for measuring phage concentration.
Article
Microbiology
Hidehito Matsui, Jumpei Uchiyama, Masaya Ogata, Tadahiro Nasukawa, Iyo Takemura-Uchiyama, Shin-ichiro Kato, Hironobu Murakami, Masato Higashide, Hideaki Hanaki
Summary: This study demonstrates that treatment with a recombinant endolysin specific to Enterococcus faecalis potentially improves the accuracy of prenatal GBS screening test by enriching Group B Streptococcus (GBS) and increasing the GBS-positive ratio. The presence of commensal bacteria such as Enterococcus faecalis in clinical specimens can inhibit GBS growth in selective enrichment culture, leading to false-negative results. The application of originally isolated endolysin in the enrichment culture inhibits unwanted E. faecalis growth, thereby avoiding false-negative results in both experimental settings and tests using vaginal swabs.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Iyo Takemura-Uchiyama, Hiroki Tsurui, Hidekatsu Shimakura, Tadahiro Nasukawa, Ichiro Imanishi, Jumpei Uchiyama, Tomoki Fukuyama, Shuji Sakamoto, Keiko Morisawa, Masato Fujimura, Hironobu Murakami, Shuji Kanamaru, Kenji Kurokawa, Keiko Kawamoto, Keita Iyori, Masahiro Sakaguchi
Summary: Staphylococcus pseudintermedius is a major pathogen causing canine skin infection, and it likely exacerbates the severity of canine atopic dermatitis (AD). This study investigated the IgE reactivities to different strains of S. pseudintermedius and identified several IgE-reactive proteins. The findings suggest that S. pseudintermedius may worsen AD in dogs differently from Staphylococcus aureus in human AD.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Veterinary Sciences
Naoyuki Aihara, Anna Momoki, Nanase Hattori, Hironobu Murakami, Motoharu Oishi, Yoshiaki Goto, Reiichiro Sato, Makoto Nagai, Kazutaka Yamada, Junichi Kamiie
Summary: This is a case of congenital malformations of the external and middle ear accompanied by temporal bone anomaly, characterized by bilateral microtia and absent external acoustic meatus.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Biochemical Research Methods
Liushiqi Borjigin, Shuji Yoneyama, Susumu Saito, Meripet Polat, Michihito Inokuma, Yasuo Shinozaki, Naoko Tanaka, Risa Yamanaka, Anna Yasui, Munehito Mimura, Hironobu Murakami, Shin-Nosuke Takeshima, Yoko Aida
Summary: The study identified four single mutations within the probe region of the original BLV-CoCoMo-qPCR assay, three of which decreased sensitivity. Modified probes were designed to match the mutant strains, resulting in an improved assay with maintained sensitivity and reproducibility.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Virology
Hironobu Murakami, Makoto Murakami-Kawai, Shinji Kamisuki, Shibasaki Hisanobu, Yukine Tsurukawa, Jumpei Uchiyama, Masahiro Sakaguchi, Kenji Tsukamoto
Summary: The study found that a compound vioE isolated from fungus can inhibit the transcriptional activity of bovine leukemia virus (BLV), suggesting a novel target for the development of anti-BLV drugs by interfering with the virus's transcriptional activation.
Article
Microbiology
Ayumi Nakatsuchi, Sonoko Watanuki, Liushiqi Borjigin, Hirotaka Sato, Lanlan Bai, Ryosuke Matsuura, Maho Kuroda, Hironobu Murakami, Reiichiro Sato, Sakurako Asaji, Asako Ando, Yasunobu Matsumoto, Shin-Nosuke Takeshima, Yoko Aida
Summary: This study revealed that BoLA-DRB3 polymorphism affects the PVL and infectivity of milk from BLV-infected dams, with susceptible dams showing significantly higher BLV provirus detection rate and PVL compared to resistant dams. The visualization of infectivity using a luminescence syncytium induction assay confirmed a markedly high infectious risk of milk from susceptible dams.
Article
Virology
Yuka Fukase, Fujiko Minami, Tsuneyuki Masuda, Toru Oi, Hitoshi Takemae, Hiroho Ishida, Hironobu Murakami, Naoyuki Aihara, Takanori Shiga, Junichi Kamiie, Tetsuya Furuya, Tetsuya Mizutani, Mami Oba, Makoto Nagai
Summary: This study investigates Japanese porcine orthoreoviruses and finds that they exhibit genetic diversity and potential zoonotic transmission. In addition, intragenetic reassortment and homologous recombination events contribute to the evolution of these viruses.
ARCHIVES OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
J. Uchiyama, T. Osumi, K. Mizukami, T. Fukuyama, A. Shima, A. Unno, I Takemura-Uchiyama, Y. Une, H. Murakami, M. Sakaguchi
Summary: This study found that atopic dermatitis can alter the oral and gut microbiota in dogs. The diversity of the oral microbiota varied significantly among the dogs, while there was no significant difference in the gut microbiota. The gut microbiota of AD dogs had a differential abundance of the Anaerovoracaceae family, while no specific bacterial taxa were detected in the oral microbiota. Furthermore, the bacteria in the oral microbiota associated with AD were related to human periodontitis, while those in the gut microbiota were associated with dysbiosis and gut inflammation.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Virology
Jumpei Uchiyama, Iyo Takemura-Uchiyama, Kazuyoshi Gotoh, Shin-ichiro Kato, Yoshihiko Sakaguchi, Hironobu Murakami, Tomoki Fukuyama, Mao Kaneki, Osamu Matsushita, Shigenobu Matsuzaki
Summary: This study examined the phylogeny of dU jumbophages, a type of phage with uracil in their DNA. Through gene analysis and gene-sharing network analysis, the researchers found that dU jumbophages are similar to each other and form a clustered group. They also discovered two uncultured phages that may be related to dU jumbophages. These findings suggest that dU jumbophages may have originated from a common source and diverged before the divergence of Gram-negative and Gram-positive bacteria.
Article
Veterinary Sciences
Sakurako Neo, Iyo Takemura-Uchiyama, Jumpei Uchiyama, Hironobu Murakami, Ayaka Shima, Hideki Kayanuma, Taiki Yokoyama, Satoshi Takagi, Eiichi Kanai, Masaharu Hisasue
Summary: This study analyzed the microbial community in the canine gallbladder and found a specific bacterial community present in the bile of diseased dogs, while no bacteria were detected in healthy dogs.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mariko Komuro, Masaki Nagane, Tomoki Fukuyama, Xiaolin Luo, Shinobu Hiraki, Masakatsu Miyanabe, Miyuki Ishikawa, Chiaki Niwa, Hironobu Murakami, Mariko Okamoto, Tadashi Yamashita
Summary: The study found that sphingomyelin plays a vital role in maintaining the cutaneous barrier through regulation of the STAT3 pathway. These results suggest that sphingomyelin could be a potential therapeutic target for treating dermatitis.
Article
Veterinary Sciences
Kanumporn Mungthong, Soe Thiri Khaing, Takehiko Otsubo, Chihiro Hatanaka, Shuji Yoneyama, Shin Hisamatsu, Hironobu Murakami, Kenji Tsukamoto
Summary: The P25 primer set was designed for broad detection of BVDV1 and BVDV2 with high sensitivity. Compared to the Aebischer primer set, the P25 primer set can detect a wider range of subgenotypes.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hirofumi Kawakubo, Shinji Kamisuki, Kei Suzuki, Jesus Izaguirre-Carbonell, Shiki Saito, Hiroshi Murata, Atsushi Tanabe, Ayumi Hongo, Hironobu Murakami, Sachihiro Matsunaga, Kengo Sakaguchi, Hiroeki Sahara, Fumio Sugawara, Kouji Kuramochi
Summary: Sulfoglycolipid, SQAP, is a radiosensitizing agent that increases the sensitivity of tumor cells to radiation therapy. In low oxygen conditions, SQAP shows higher efficacy in inhibiting the growth of hepatocarcinoma cells and affecting the expression of key proteins.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Tadahiro Nasukawa, Ryosuke Sugimoto, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Hironobu Murakami, Ken Fukuda, Shigenobu Matsuzaki, Masahiro Sakaguchi
Summary: In this study, high-performance flow cytometry was used to analyze membrane vesicles (MVs) from Gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis, showing that flow cytometric sorting provided higher purity and uniformity compared to gradient isolation alone. The MV purification method using flow cytometry should be useful for applications requiring very high purity of MV samples such as proteomic, metagenomic, or lipidomic studies.
RESEARCH IN MICROBIOLOGY
(2021)
