Article
Cell Biology
Megan B. Stemberger, Julia A. Ju, Keyata N. Thompson, Trevor J. Mathias, Alexandra E. Jerrett, Katarina T. Chang, Eleanor C. Ory, David A. Annis, Makenzy L. Mull, Darin E. Gilchrist, Michele I. Vitolo, Stuart S. Martin
Summary: Levels of hydrogen peroxide are higher in breast tumor microenvironment. Hydrogen peroxide mediates breast carcinogenesis and metastasis. The study shows the effects of exposure to elevated hydrogen peroxide on breast cancer cells.
Article
Cell Biology
Jan Martinek, Jianan Lin, Kyung In Kim, Victor G. Wang, Te-Chia Wu, Michael Chiorazzi, Hannah Boruchov, Ananya Gulati, Shamreethaa Seeniraj, Lili Sun, Florentina Marches, Paul Robson, Anthony Rongvaux, Richard A. Flavell, Joshy George, Jeffrey H. Chuang, Jacques Banchereau, Karolina Palucka
Summary: Modulating immune function at the tumor site could improve patient outcomes. This study shows that tumor infiltrating T cells are predominantly associated with CD14(+) monocytes/macrophages rather than melanoma cells in metastatic melanoma patients. Transcriptional analysis reveals that stromal CD14(+) cells have a distinct signature compared to CD14(+) cells within tumor nests and the included LY75 gene may serve as a potential biomarker for predicting prolonged survival in metastatic cutaneous melanoma and other cancers. Reprogramming stromal macrophages to acquire dendritic cell (DC) function could offer a therapeutic opportunity for metastatic cancers.
CELL REPORTS MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yun Zhao, Xiaoxia Peng, Hope Baldwin, Chao Zhang, Zhongmin Liu, Xin Lu
Summary: Despite advancements in AR antagonists, CRPC remains incurable and requires further study in animal models. Prostate-specific deletion of Pten and Smad4 in mice mimics human prostate adenocarcinoma, with Pten/Smad4 tumors promoting an immunosuppressive microenvironment. Combination therapy of surgical castration and enzalutamide in Pten/Smad4 mice slows tumor growth and prolongs survival. RNA profiling reveals transcriptomic alterations in CRPC, particularly related to immune-related and coagulation-related pathways in lung metastatic CRPC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Multidisciplinary Sciences
Natalia Kulach, Ewelina Pilny, Tomasz Cichon, Justyna Czapla, Magdalena Jarosz-Biej, Marek Rusin, Alina Drzyzga, Sybilla Matuszczak, Stanislaw Szala, Ryszard Smolarczyk
Summary: The study demonstrated that MSC are effective carriers of IL-12 cytokine, showing anti-tumor effects in primary and metastatic melanoma models. The use of MSC as carriers allowed for intravenous administration of IL-12 without toxic side effects, resulting in reduced tumor growth and metastases, decreased vascular density, and increased immune response in treated mice. Therapeutic properties of IL-12 cytokine were successfully exerted by modified MSC without inducing toxicity.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Xiaodong Wang, Bingying Yu, Birong Cao, Ji Zhou, Yongqiang Deng, Zhulin Wang, Guangyi Jin
Summary: The immunogenic BRD4 inhibitor SZU-119 designed in this study demonstrated potent antitumor activity, suppressing tumor growth, prolonging survival time of mice, promoting cytokine production and immune cell activation, and enhancing CTL cytotoxicity in a mouse melanoma model. This work provides new insights for the development of anti-melanoma drugs targeting both innate and adaptive immunity.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Yoon-Seob Kim, Sun Shin, Jing Hu Yin, Junseong Park, Seung-Hyun Jung, Yeun-Jun Chung
Summary: This study provides insight into the mechanism of melanoma metastasis at the single-cell level. Differential gene expression analysis identified upregulated genes related to metastasis and downregulated genes in the Mitf pathway. The proportion of cycling cells and high expression of specific genes were closely associated with the metastatic ability of melanoma cells. The evolution of cell populations during metastasis showed distinct characteristics and unique metastatic potential. Genomic alterations, including shared mutations and specific changes, contributed to the development of metastasis in melanoma.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Dermatology
Qi Jia, Li Tao, Yinyin Zhou, Li Song, Zhonghong Wei, Tao Lu, James R. Woodgett, Yin Lu
Summary: The study found that the GSK-3 inhibitor Pym-5 can delay the growth of melanoma cells, promote melanin production, and achieve this effect by activating the Wnt/β-Catenin signaling pathway.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Oncology
Adriana Taveira da Cruz, Aline Hunger, Fabiana Henriques Machado de Melo, Ana Carolina Monteiro, Genevieve Catherine Pare, Dulce Lai, Debora Kristina Alves-Fernandes, Ana Luisa Pedroso Ayub, Esteban Mauricio Cordero, Jose Franco da Silveira Filho, Regine Schneider-Stock, Bryan Eric Strauss, Victor Tron, Miriam Galvonas Jasiulionis
Summary: The deregulation of miRNAs plays a role in the development of various types of cancer, including melanoma. In a study on mice melanoma cells, overexpression of miR-138-5p was found to induce an aggressive phenotype, leading to increased proliferation, migration, and stress resistance. Furthermore, the overexpression of miR-138-5p was linked to the direct suppression of the tumor suppressor Trp53, contributing to melanoma metastasis.
Article
Biochemistry & Molecular Biology
Jana Jandova, Sophia L. Park, Mandi J. Corenblum, Lalitha Madhavan, Jeremy A. Snell, Liliana Rounds, Georg T. Wondrak
Summary: The study suggests that the clinical antimalarial drug mefloquine may be an effective candidate for drug repurposing to eliminate malignant melanoma cells, even when metastasized to the brain and resistant to BRAF kinase inhibitors.
MOLECULAR CARCINOGENESIS
(2022)
Article
Cell Biology
Katja Piaszinski, Martina Rincic, Thomas Liehr, Shaymaa Azawi
Summary: The study revealed genetic rearrangements in melanoma cell lines, involving oncogenes and tumor suppressor genes associated with aggressive forms of melanoma in humans. These cell lines can be considered as model systems for advanced eye and skin melanoma, aiding research into the molecular mechanisms and potential treatments for melanoma.
CYTOGENETIC AND GENOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Daiane Manica, Gilnei Bruno da Silva, Jussara de Lima, Joana Cassol, Paula Dallagnol, Rafael Antonio Narzetti, Marcelo Moreno, Margarete Dulce Bagatini
Summary: This study evaluated the effects of caffeine on melanoma cells. The results showed that caffeine can reduce cell viability and migration, induce the generation of reactive oxygen species (ROS), and decrease the expression of CD39 and CD73. Additionally, caffeine increased extracellular ATP levels.
PURINERGIC SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Ludmila Alekseeva, Aleksandra Sen'kova, Innokenty Savin, Marina Zenkova, Nadezhda Mironova
Summary: The study showed that recombinant human DNase I Pulmozyme effectively inhibited lung metastases in the B16 melanoma model in mice, demonstrating strong antimetastatic activity both in vitro and in vivo. Additionally, Pulmozyme administration enhanced blood serum DNase activity, decreased cfDNA concentrations, efficiently degraded SINE and LINE repeats, and induced apoptosis in metastatic foci.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)