Article
Biochemistry & Molecular Biology
Yukihiro Sera, Tsuneo Imanaka, Masafumi Yamaguchi
Summary: SDS is a genetic disorder caused by mutations in the SBDS gene, which is involved in ribosome biogenesis. The interaction between SBDS and RNF2 at different stages of the cell cycle may play a role in mitotic progression.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Sandro M. Meier, Ana-Maria Farcas, Anil Kumar, Mahdiye Ijavi, Robert T. Bill, Joerg Stelling, Eric R. Dufresne, Michel O. Steinmetz, Yves Barral
Summary: This study investigates the interactions and functions of the Kar9 network in yeast cells, finding that it forms a liquid condensate at selected microtubule ends, serving as a mechanical coupling between microtubules and actin cables.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Penelope Darnat, Angelique Burg, Jeremy Salle, Jerome Lacoste, Sophie Louvet-Vallee, Michel Gho, Agnes Audibert
Summary: The Frizzled/Dishevelled planar cell polarity pathway is involved in coordinating mitotic spindle orientation with the cell cycle. The study reveals that Cyclin A is recruited by Frizzled/Dishevelled in prophase to regulate division orientation in Drosophila sensory organ precursor cells. This finding provides insights into the crucial coordination between cell proliferation, cell polarity, and spindle orientation.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Alex F. Thompson, Patrick R. Blackburn, Noah S. Arons, Sarah N. Stevens, Dusica Babovic-Vuksanovic, Jane B. Lian, Eric W. Klee, Jason Stumpff
Summary: This study reveals that mutations in KIF22 disrupt chromosome segregation in anaphase, leading to reduced proliferation and abnormal cell morphology. It demonstrates the significance of regulating KIF22 activity and maintaining force balance in anaphase.
Article
Cell Biology
Diana Campos-Iglesias, Julia M. Fraile, Gabriel Bretones, Alejandro A. Montero, Elena Bonzon-Kulichenko, Jesus Vazquez, Carlos Lopez-Otin, Jose M. P. Freije
Summary: USP49 deubiquitinase is identified as a novel regulator of the spindle checkpoint. Loss of USP49 impairs proliferation and increases aneuploidy in cancer cell lines. USP49-depleted cells can overcome SAC-induced arrest in presence of nocodazole.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Rachel L. Belote, Daniel Le, Ashley Maynard, Ursula E. Lang, Adriane Sinclair, Brian K. Lohman, Vicente Planells-Palop, Laurence Baskin, Aaron D. Tward, Spyros Darmanis, Robert L. Judson-Torres
Summary: This study constructed a single-cell transcriptional map of primary human epidermal melanocytes, revealing distinct subpopulations, dedifferentiation patterns associated with melanoma prognosis, and unique cellular origins of acral melanoma. The research identified anatomical site-specific enrichment of melanocyte subpopulations during gestation that persisted into adulthood, as well as transcriptional programs defining melanocyte differentiation in humans differ from model systems. These differentiation patterns can predict melanoma prognosis and response to immune checkpoint inhibitor therapy.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shu-Ching Huang, Long V. Vu, Faye H. Yu, Dan T. Nguyen, Edward J. Benz
Summary: The unequal distribution of Numb plays a critical role in cell diversification during asymmetric cell division, which is regulated by spindle orientation. 4.1R functions as a member of the NuMA-LGN-dynein/dynactin complex and interacts with NuMA to regulate spindle orientation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Mariko Morii, Sho Kubota, Chizu Hasegawa, Yumi Takeda, Shiori Kometani, Kyoko Enomoto, Takayuki Suzuki, Sayuri Yanase, Rika Sato, Aki Akatsu, Kensuke Hirata, Takuya Honda, Takahisa Kuga, Takeshi Tomonaga, Yuji Nakayama, Noritaka Yamaguchi, Naoto Yamaguchi
Summary: The Src-family tyrosine kinases (SFKs) accumulate in the centrosome region at the beginning of mitosis and phosphorylate PRC1 and kinastrin, leading to their delocalization from microtubules during mitosis.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Imge Ozugergin, Alisa Piekny
Summary: Cytokinesis, the process of physically dividing a cell into two daughters, has been extensively studied in vitro and early embryos, but its regulation in different animal cell types and developmental contexts remains poorly understood. Recent studies have revealed striking differences in the regulation of cytokinesis between different cell types and organisms, including diverse threshold requirements for structural components and different mechanisms of regulation. This review focuses on these differences, particularly in pathways independent of the mitotic spindle, and associated with the cortex, kinetochores, or chromatin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anne Nehlig, Cynthia Seiler, Yulia Steblyanko, Florent Dingli, Guillaume Arras, Damarys Loew, Julie Welburn, Claude Prigent, Marin Barisic, Clara Nahmias
Summary: Depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. The absence of ATIP3 leads to the accumulation of Kif2A and Dda3 at spindle poles, affecting poleward microtubule flux and spindle shortening. ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size, highlighting their mutual regulation in controlling spindle length.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wangxi Luo, Vladimir Demidov, Qi Shen, Hugo Girao, Manas Chakraborty, Aleksandr Maiorov, Fazly I. Ataullakhanov, Chenxiang Lin, Helder Maiato, Ekaterina L. Grishchuk
Summary: CLASPs are universal stabilizers of microtubule dynamics, and this study reveals that clusters of human CLASP2 form a load-bearing bond with terminal non-GTP tubulins at the stabilized microtubule tip. This activity relies on the TOG2 domain of CLASP2, which releases its high-affinity bond with non-GTP tubulins when they convert into polymerization-competent GTP-tubulins. The recognition of nucleotide-specific tubulin conformation by CLASP2 and its ability to stabilize non-GTP tubulins contribute to the suppression of catastrophe at freely assembling microtubule ends and the promotion of persistent tubulin assembly at load-bearing tethered ends.
Article
Cell Biology
Yaqian Zhang, Xing Hong, Shasha Hua, Kai Jiang
Summary: In this study, the authors investigated the mechanism of branching microtubule nucleation. They found that human augmin complex and gamma-tubulin ring complex (gamma-TuRC) are sufficient to reconstitute the minimal machinery for branching microtubules and that NEDD1 plays a key role in bridging between these two complexes. They also discovered that phosphorylation by CDK1 and PLK1 is crucial for the binding of NEDD1 to augmin and for branching microtubule nucleation.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
May Wathone Oo, Hotaka Kawai, Kiyofumi Takabatake, Shuta Tomida, Takanori Eguchi, Kisho Ono, Qiusheng Shan, Toshiaki Ohara, Saori Yoshida, Haruka Omori, Shintaro Sukegawa, Keisuke Nakano, Kuniaki Okamoto, Akira Sasaki, Hitoshi Nagatsuka
Summary: Research has found that oral cancer stroma recruits CCR2(+) MDSCs from the bone marrow to the tumor microenvironment through the secretion of CCL2.
Article
Cell & Tissue Engineering
Joshua T. Cohen, Michael Danise, Jason T. Machan, Runping Zhao, Craig T. Lefort
Summary: Transplantation of NPs was found to decrease the expression of inhibitory factors in host neutrophils and reduce proinflammatory cytokines in the lungs after shock, increasing neutrophil migration and enhancing bacterial clearance, indicating the potential of NPs as a cellular therapy for secondary infection following hemorrhagic shock.