Article
Biotechnology & Applied Microbiology
Maral Tsevelkhoroloo, Vijayalakshmi Dhakshnamoorthy, Young-Soo Hong, Chang-Ro Lee, Soon-Kwang Hong
Summary: In this study, two α-neoagarooligosaccharide hydrolases (SCO3481 and SCO3479) were identified in Streptomyces coelicolor A3(2), which catalyzed the hydrolysis of α-(1,3) glycosidic bonds. SCO3481 belonged to the GH117 family and formed dimers, while SCO3479 was a large monomeric α-neoagarooligosaccharide hydrolase belonging to the GH2 family with β-galactosidase activity. SCO3479, a unique bifunctional enzyme, has many advantages for industrial applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Kazuki Ishikawa, Makoto Hashimoto, Kunpei Komatsu, Takaaki Taguchi, Susumu Okamoto, Koji Ichinose
Summary: Actinorhodin (ACT) is an antibiotic produced by Streptomyces coelicolor A3(2), which has been widely studied as a model organism for antibiotic biosynthesis and regulation. The study found that ActVI-ORF2 is a stereospecific enoyl reductase that converts (S)-DNPA to DDHK.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Maurice Finger, Fabio Sentek, Lukas Hartmann, Ana M. Palacio-Barrera, Ivan Schlembach, Miriam A. Rosenbaum, Jochen Buechs
Summary: In this study, high-throughput online monitoring was used to gain a deep understanding of the cultivation process of the model organism Streptomyces coelicolor A3(2). By measuring the oxygen transfer rate and autofluorescence online, valuable information about nutrient availability and pigment product formation patterns can be obtained. The study identified the filling volume of the cultivation vessel as a factor influencing pigment formation.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Microbiology
Maral Tsevelkhoroloo, So Heon Shim, Chang-Ro Lee, Soon-Kwang Hong, Young-Soo Hong
Summary: This study elucidated the role of DagR in regulating the expression of three agarase genes in S. coelicolor A3(2) by developing a dagR-deletion mutant. The Delta sco3485 mutant showed increased mRNA levels of agarolytic pathway genes and higher agarase production, indicating that DagR encodes a repressor that inhibits gene expression at the transcriptional level. Unique inhibition of DNA-binding activity of DagR by AHG was observed in this study.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shumpei Horii, Ashok Zachariah Samuel, Takuji Nakashima, Akira Take, Atsuko Matsumoto, Yoko Takahashi, Masahiro Ando, Haruko Takeyama
Summary: This study used Raman microspectroscopic imaging to investigate the correlation between avermectin production and morphological differentiation in Streptomyces avermitilis. The results showed distinct variations in the localization of secondary metabolites at different stages of morphological differentiation. Avermectin was detected in the later stages of differentiation under solid culture, but concentrated in the early stages under liquid culture. The chemical profiles of the mycelia also differed significantly depending on the culture condition.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jeremy Dufourt, Maelle Bellec, Antonio Trullo, Matthieu Dejean, Sylvain De Rossi, Cyril Favard, Mounia Lagha
Summary: By using the SunTag method, researchers visualized and quantified the translation of single mRNAs in living Drosophila embryos, revealing spatial heterogeneity in mRNA translation efficiency and the presence of translation factories. These findings open up new avenues for understanding the role of gene regulation during development in living multicellular organisms.
Article
Biotechnology & Applied Microbiology
Maral Tsevelkhorloo, Sang Hoon Kim, Dae-Kyung Kang, Chang-Ro Lee, Soon-Kwang Hong
Summary: Agarose is a linear polysaccharide made up of D-galactose and 3,6-anhydro-L-galactose and is a major component of red algal cell walls. The obstacle in large-scale use of agarose is the inability to ferment AHG. Understanding AHG metabolism in agarolytic microorganisms could help overcome this obstacle.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Enerand Mackon, Yafei Ma, Guibeline Charlie Jeazet Dongho Epse Mackon, Qiufeng Li, Qiong Zhou, Piqing Liu
Summary: In this study, the subcellular localization of anthocyanin in black and brown rice stigma cells was investigated using confocal microscopy. The results revealed the presence of two pools of anthocyanin in the cells, with different structures and sizes, indicating various transport mechanisms of anthocyanin from the cytoplasm to the central vacuole. These findings provide important insights into anthocyanin metabolism, sequestration, and trafficking in black rice.
Article
Microbiology
Nathaniel D. M. Holman, Anthony J. Wilkinson, Margaret C. M. Smith
Summary: In this study, the structure and function of S. coelicolor Pmt were investigated by mutating highly conserved residues. The results showed that substitutions of certain conserved residues led to loss of Pmt function, highlighting the importance of residue conservation for enzyme activity.
Article
Multidisciplinary Sciences
Ana Paula Thiesen, Bruna Mielczarski, Ricardo Francalacci Savaris
Summary: This study aimed to compare the protein expression of BGN in breast tissue with and without cancer using immunohistochemistry and deep learning algorithms. The results showed that BGN protein expression was significantly reduced in breast cancer tissue compared to normal tissue.
Article
Microbiology
Georgia R. Squyres, Matthew J. Holmes, Sarah R. Barger, Betheney R. Pennycook, Joel Ryan, Victoria T. Yan, Ethan C. Garner
Summary: This study used live-cell single-molecule imaging to reveal that the divisome consists of two subcomplexes, showing that different components move differently. FtsZ-binding proteins were found to play a crucial role in bundling FtsZ filaments together to form Z rings, which are essential for cytokinesis.
NATURE MICROBIOLOGY
(2021)
Article
Cell Biology
Igor Kukhtevich, Mariana Rivero-Romano, Namisha Rakesh, Poonam Bheda, Yagya Chadha, Paulina Rosales-Becerra, Stephan Hamperl, Daniela Bureik, Scarlett Dornauer, Catherine Dargemont, Antonis Kirmizis, Kurt M. Schmoller, Robert Schneider
Summary: RNA distribution during cell division is asymmetric and regulated by factors such as the lifespan regulator Sir2 and replicative age. This study establishes an approach to quantify RNA dynamics in single cells using the diSpinach aptamer in a microfluidics device and validates it using budding yeast as a model.
Article
Biochemistry & Molecular Biology
Seokran Go, Dokyung Jeong, Jinkyoung Chung, Geun-Ho Kim, Jaewoo Song, Eunyoung Moon, Yang Hoon Huh, Doory Kim
Summary: This research examines the ultrastructural organization of various organelles in different platelet intermediates using super-resolution microscopy. The study reveals that microtubules control the overall shape of platelet intermediates, while actin and microtubules regulate the distribution of organelles. These findings call for a reassessment of organelle redistribution in platelet intermediates.
Article
Cell Biology
Takahiro K. Fujiwara, Taka A. Tsunoyama, Shinji Takeuchi, Ziya Kalay, Yosuke Nagai, Thomas Kalkbrenner, Yuri L. Nemoto, Limin H. H. Chen, Akihiro C. E. Shibata, Kokoro Iwasawa, Ken P. Ritchie, Kenichi G. N. Suzuki, Akihiro Kusumi
Summary: The ultrafast camera developed by Fujiwara et al. has significantly improved the time resolution of single-molecule localization microscopy, revealing the dynamic nano-architecture of focal adhesions and leading to the model of compartmentalized focal adhesion protein islands.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Surgery
Fernando Dip, Piero Francesco Alesina, Angkoon Anuwong, Eham Arora, Eren Berber, Jamie Bonnin-Pascual, Nicole D. Bouvy, Marco Stefano Demarchi, Jorge Falco, Klaus Hallfeldt, Kang Dae Lee, Melanie L. Lyden, Christina Maser, Edwina Moore, Theodossis Papavramidis, John Phay, Jose M. Rodriguez, Barbara Seeliger, Carmen C. Solorzano, Frederic Triponez, Alexander Vahrmeijer, Raul J. Rosenthal, Kevin P. White, Michael Bouvet
Summary: Fluorescence imaging has potential advantages in thyroid and parathyroid surgery, but further research is needed to optimize the dose and timing of the fluorescent dye.
Article
Biochemistry & Molecular Biology
Michelle A. Schorn, Stefan Verhoeven, Lars Ridder, Florian Huber, Deepa D. Acharya, Alexander A. Aksenov, Gajender Aleti, Jamshid Amiri Moghaddam, Allegra T. Aron, Saefuddin Aziz, Anelize Bauermeister, Katherine D. Bauman, Martin Baunach, Christine Beemelmanns, J. Michael Beman, Maria Victoria Berlanga-Clavero, Alex A. Blacutt, Helge B. Bode, Anne Boullie, Asker Brejnrod, Tim S. Bugni, Alexandra Calteau, Liu Cao, Victor J. Carrion, Raquel Castelo-Branco, Shaurya Chanana, Alexander B. Chase, Marc G. Chevrette, Leticia V. Costa-Lotufo, Jason M. Crawford, Cameron R. Currie, Bart Cuypers, Tam Dang, Tristan de Rond, Alyssa M. Demko, Elke Dittmann, Chao Du, Christopher Drozd, Jean-Claude Dujardin, Rachel J. Dutton, Anna Edlund, David P. Fewer, Neha Garg, Julia M. Gauglitz, Emily C. Gentry, Lena Gerwick, Evgenia Glukhov, Harald Gross, Muriel Gugger, Dulce G. Guillen Matus, Eric J. N. Helfrich, Benjamin-Florian Hempel, Jae-Seoun Hur, Marianna Iorio, Paul R. Jensen, Kyo Bin Kang, Leonard Kaysser, Neil L. Kelleher, Chung Sub Kim, Ki Hyun Kim, Irina Koester, Gabriele M. Koenig, Tiago Leao, Seoung Rak Lee, Yi-Yuan Lee, Xuanji Li, Jessica C. Little, Katherine N. Maloney, Daniel Maennle, Christian Martin H., Andrew C. McAvoy, Willam W. Metcalf, Hosein Mohimani, Carlos Molina-Santiago, Bradley S. Moore, Michael W. Mullowney, Mitchell Muskat, Louis-Felix Nothias, Ellis C. O'Neill, Elizabeth I. Parkinson, Daniel Petras, Jorn Piel, Emily C. Pierce, Karine Pires, Raphael Reher, Diego Romero, M. Caroline Roper, Michael Rust, Hamada Saad, Carmen Saenz, Laura M. Sanchez, Soren Johannes Sorensen, Margherita Sosio, Roderich D. Sussmuth, Douglas Sweeney, Kapil Tahlan, Regan J. Thomson, Nicholas J. Tobias, Amaro E. Trindade-Silva, Gilles P. van Wezel, Mingxun Wang, Kelly C. Weldon, Fan Zhang, Nadine Ziemert, Katherine R. Duncan, Max Cruesemann, Simon Rogers, Pieter C. Dorrestein, Marnix H. Medema, Justin J. J. van der Hooft
Summary: Genomics and metabolomics are commonly used to explore the diversity of specialized metabolites. The Paired Omics Data Platform aims to systematically document the connections between metabolome and (meta)genome data to aid in the identification of natural product biosynthetic origins and metabolite structures.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Microbiology
Sanne Westhoff, Alexander M. Kloosterman, Stephan F. A. van Hoesel, Gilles P. van Wezel, Daniel E. Rozen
Summary: Bacteria compete for resources and space by producing antibiotics that inhibit competitors, with antibiotic production influenced by social cues and resource limitation. Antibiotic production is more likely to be induced by cues from closely related strains or those sharing biosynthetic gene clusters, but not by competitors that inhibit growth. Cells often reduce antibiotic production in the presence of competitors, especially under nutrient limitation, highlighting the regulation of antibiotic production by interactions between social and resource environments.
Article
Biochemistry & Molecular Biology
Xiansha Xiao, Joost Willemse, Patrick Voskamp, Xinmeng Li, Andrea E. Prota, Meindert Lamers, Navraj Pannu, Jan Pieter Abrahams, Gilles P. van Wezel
Summary: The study found that amino acid substitutions in the SsgB protein of Streptomyces bacteria led to ectopically placed septa and diagonally or longitudinally divided cells. This suggests that besides altering FtsZ, aa substitutions in SsgB also play a role in cell division regulation in Streptomyces.
Review
Biochemistry & Molecular Biology
Mariana Avalos, Paolina Garbeva, Lisa Vader, Gilles P. van Wezel, Jeroen S. Dickschat, Dana Ulanova
Summary: Terpenoids are the largest class of natural products known to date, found in both plants and microorganisms. They play important roles in microbial chemical ecology, including stress alleviation, maintenance of cell membrane integrity, and photoprotection.
NATURAL PRODUCT REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Mandy B. Hulst, Thadee Grocholski, Jacques J. C. Neefjes, Gilles P. van Wezel, Mikko Metsa-Ketela
Summary: Doxorubicin, widely used in cancer treatment but limited by its severe side-effects, may be surpassed by new anthracycline-based drugs that focus on histone eviction activity to avoid cardiac toxicity. Recent advancements in biosynthesis and discovery of new congeners from rare Actinobacteria have laid the foundation for the development of novel anthracyclines.
NATURAL PRODUCT REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Doris A. van Bergeijk, Somayah S. Elsayed, Chao Du, Isabel Nunez Santiago, Anna M. Roseboom, Le Zhang, Victor J. Carrion, Herman P. Spaink, Gilles P. van Wezel
Summary: The biosynthetic gene clusters of Actinobacteria are poorly expressed in the laboratory. However, the stress hormone epinephrine has been found to promote their expression and influence the metabolism of Streptomyces. This response is mediated by the catechol moiety.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Microbiology
Xiaobo Zhong, Le Zhang, Gilles P. van Wezel, Erik Vijgenboom, Dennis Claessen
Summary: Lytic polysaccharide monooxygenases are commonly used in industry for efficient degradation of difficult-to-degrade polysaccharides. However, recently it has been discovered that these enzymes also play important biological roles, such as remodeling the bacterial cell wall. This newly identified biological role may be widespread due to the common presence of lytic polysaccharide monooxygenases in microbes.
Article
Microbiology
Chao Du, Joost Willemse, Amanda M. Erkelens, Victor J. Carrion, Remus T. Dame, Gilles P. van Wezel
Summary: This study identified a novel nucleoid-associated protein (NAP), Gbn, in Actinobacteria that binds to GATC sequences and plays a significant role in the growth and development of Streptomyces. Gbn has broad effects on global gene expression during the late developmental stage.
Article
Multidisciplinary Sciences
Renee Kapteijn, Shraddha Shitut, Dennis Aschmann, Le Zhang, Marit de Beer, Deniz Daviran, Rona Roverts, Anat Akiva, Gilles P. van Wezel, Alexander Kros, Dennis Claessen
Summary: A new endocytosis-like process has been discovered in cell wall-deficient bacteria, allowing them to engulf DNA and other extracellular materials. This process differs from the traditional mechanisms of horizontal gene transfer and requires energy. The findings suggest a possible mechanism for early life forms to acquire essential resources before the development of the bacterial cell wall.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Paula Yague, Joost Willemse, Xiansha Xiao, Le Zhang, Angel Manteca, Gilles P. van Wezel
Summary: The GTPase FtsZ forms the cell division scaffold in bacteria. Streptomycetes undergo two different forms of cell division. In a previous study, FtsZ was found to be phosphorylated at Ser 317 and Ser389, resulting in changes in antibiotic production.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Le Zhang, Joost Willemse, Paula Yague, Ellen de Waal, Dennis Claessen, Gilles P. van Wezel
Summary: Bacterial cell division begins with the polymerization of FtsZ and the formation of a cell division scaffold. SepF aligns FtsZ polymers and anchors the Z-ring to the membrane. Mutations in SflA and SflB in Streptomyces lead to branching of aerial hyphae and abnormal localization of DivIVA. Overexpression of SflA and SflB inhibits hyphal growth and correlates with the activity of DivIVA. The findings suggest that SflA and SflB play an important role in growth and cell division control during Streptomyces development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Alexander T. Bakker, Ioli Kotsogianni, Liza Mirenda, Verena M. Straub, Mariana Avalos, Richard J. B. H. N. van den Berg, Bogdan I. Florea, Gilles P. van Wezel, Antonius P. A. Janssen, Nathaniel I. Martin, Mario van der Stelt
Summary: Phenotypic screening identifies a series of 1,3,4-oxadiazole-3-ones as novel antibacterial compounds with a polypharmacological mode of action against multidrug-resistant Staphylococcus aureus. Activity-based protein profiling reveals several cysteine and serine hydrolases as relevant targets. This study highlights oxadiazolones as a promising antibacterial chemotype with central roles of FabH, FphC, and AdhE.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
Rongbin Wang, Jennifer Nguyen, Jacob Hecht, Nora Schwartz, Katelyn Brown, Larissa Ponomareva, Magdalena Niemczura, Dino van Dissel, Gilles P. van Wezel, Jon S. Thorson, Mikko Metsa-Ketela, Khaled A. Shaaban, S. Eric Nybo
Summary: By developing the BIOPOLYMER toolbox, we have overcome the limitations of slow growth and lack of genetic tools in actinomycetes for the synthesis of anthracyclinones. We successfully improved the production host and optimized the production of anthracyclinones, identifying optimal combinations for different molecules. Additionally, we demonstrated the moderate cytotoxicity of the anthracyclinones against multiple cancer cell lines.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Medicinal
Karol Al Ayed, Denise Zamarbide Losada, Nataliia Machushynets, Barbara Terlouw, Somayah S. Elsayed, Julian Schill, Vincent Trebosc, Michel Pieren, Marnix H. Medema, Gilles P. van Wezel, Nathaniel I. Martin
Summary: The rise of antibiotic resistance calls for the development of new classes of antibiotics, such as the recently discovered relacidines, which show promising activity against Gram-negative pathogens. In this study, a bioinformatics guided total synthesis of relacidine A and B was reported, confirming the predicted and synthesized structures. Furthermore, a synthetic analogue of relacidine A with enhanced hydrolytic stability was found to maintain antibacterial activity in both in vitro and in vivo studies.
ACS INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Chaoxian Bai, Gilles P. van Wezel
Summary: Streptomyces, a genus of Gram-positive bacteria, is a valuable producer of natural products with significant benefits for human health, agriculture, and biotechnology. Researchers have developed the CUBIC system, which allows efficient and inducible gene knockdown in Streptomyces, demonstrating its versatility and reliability.
ACS SYNTHETIC BIOLOGY
(2023)
