Article
Microbiology
Jessie Fernandez, Victor Lopez, Lisa Kinch, Mariel A. Pfeifer, Hillery Gray, Nalleli Garcia, Nick Grishin, Chang-Hyun Khang, Kim Orth
Summary: The study identified two metalloproteinase enzymes, MoMca1 and MoMca2, in M. oryzae, which play significant roles in fungal growth and development, potentially regulating stress responses. The absence of these enzymes resulted in delays in fungal morphogenesis and insights into the mechanisms of M. oryzae pathogenicity.
Article
Ecology
Kuleshwar Prasad Sahu, Aundy Kumar, Asharani Patel, Mukesh Kumar, S. Gopalakrishnan, G. Prakash, R. Rathour, Robin Gogoi
Summary: Dark brown necrotic lesions caused by Magnaporthe oryzae on rice foliage provide a contrasting microhabitat for leaf-colonizing microbiome, with 17 bacterial species identified in these lesions showing biocontrol potential against the pathogen. Over 50% of the bacterial isolates were able to suppress the growth of M. oryzae, with certain strains triggering the expression of defense genes in rice, indicating their potential as biocontrol agents for managing rice blast disease.
Article
Genetics & Heredity
Vishesh Kumar, Priyanka Jain, Sureshkumar Venkadesan, Suhas Gorakh Karkute, Jyotika Bhati, Malik Zainul Abdin, Amitha Mithra Sevanthi, Dwijesh Chandra Mishra, Krishna Kumar Chaturvedi, Anil Rai, Tilak Raj Sharma, Amolkumar U. Solanke
Summary: This study analyzed the transcriptome of rice plants in response to panicle blast infection, identifying key genes involved in defense response, cell wall strengthening pathways, and potential mechanisms of panicle blast resistance. Understanding these mechanisms could aid in breeding resistant rice varieties.
Article
Microbiology
Pengyun Huang, Huijuan Cao, Yan Li, Siyi Zhu, Jing Wang, Qing Wang, Xiaohong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study reveals the functional diversity and complexity of melanin in different strains of Magnaporthe oryzae. Melanin promotes spore production in the rice blast pathogen, but its effect is related to the background melanin content of the wild-type strains. Transcription factors like Cnf1 have opposite effects on conidiophore differentiation in different strains. Pig1 regulates melanin synthesis in hyphae but not in conidiophores, spores, or appressoria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Kanyanat Lamanchai, Nicholas Smirnoff, Deborah L. Salmon, Athipat Ngernmuen, Sittiruk Roytrakul, Kantinan Leetanasaksakul, Suthathip Kittisenachai, Chatchawan Jantasuriyarat
Summary: This study investigates the role of the OsVTC1-1 gene in rice blast fungus inoculation and finds that the gene regulates rice blast resistance through multiple defense mechanisms, including hormone synthesis and signaling pathways.
Article
Multidisciplinary Sciences
Alejandra Martinez-D'Alto, Xia Yan, Tyler C. Detomasi, Richard I. Sayler, William C. Thomas, Nicholas J. Talbot, Michael A. Marletta
Summary: Blast disease caused by the fungus Magnaporthe oryzae leads to significant crop loss in cereal plants. The study shows that the expression of the putative polysaccharide monooxygenase MoPMO9A is increased during infection. MoPMO9A exhibits activity on cereal-derived mixed β-D-glucans and its domain architecture is different from other characterized AA9 PMOs. The results suggest that MoPMO9A plays a role in MBG degradation during plant infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Mohammed Javed, Bhaskar Reddy, Neelam Sheoran, Prakash Ganesan, Aundy Kumar
Summary: This study identified the roles of miRNAs and their target genes in different rice genotypes with resistance or susceptibility to blast disease. Significant differences were found between the resistant and susceptible genotypes, as well as several shared miRNAs and novel miRNAs. These findings provide valuable insights for the development of resistant rice varieties and can improve crop management practices to ensure global food security.
Article
Agronomy
Ferran Sanchez-Sanuy, Roberto Mateluna-Cuadra, Keisuke Tomita, Kazunori Okada, Gian Attilio Sacchi, Sonia Campo, Blanca San Segundo
Summary: Exposure to high iron enhances resistance to Magnaporthe oryzae infection in rice plants, possibly through upregulating defense-related genes and increasing production of phytoalexins. The infection also modulates the expression of genes involved in iron homeostasis. Iron and reactive oxygen species colocalize at infection sites. Understanding the crosstalk between iron signaling and immune signaling holds great potential for improving blast resistance in rice.
Review
Agriculture, Multidisciplinary
Hai-feng Zhang, Tofazzal Islam, Wen-de Liu
Summary: This article introduces and summarizes the classification, geographical distribution, host range, disease symptoms, biology and ecology, economic impact, and integrated pest management (IPM) program of rice and wheat blast diseases.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Gobong Choi, Jongbum Jeon, Hyunjun Lee, Shenxian Zhou, Yong-Hwan Lee
Summary: This study identified novel lncRNAs during host infection of the model pathogenic fungus Magnaporthe oryzae and revealed their potential roles in pathogenesis-related gene regulation.
Article
Agronomy
Jin-Feng Chen, Zhi-Xue Zhao, Yan Li, Ting-Ting Li, Yong Zhu, Xue-Mei Yang, Shi-Xin Zhou, He Wang, Ji-Qun Zhao, Mei Pu, Hui Feng, Jing Fan, Ji-Wei Zhang, Yan-Yan Huang, Wen-Ming Wang
Summary: The study found that rice microRNA159a plays a positive role in rice resistance to rice blast disease by regulating target genes to modulate the immune response. Proper expression of Osa-miR159a is crucial for coordinating rice blast resistance with grain development.
Article
Genetics & Heredity
Bai Li, Zhongkai Chen, Huizhen Chen, Chunlei Wang, Liyan Song, Yue Sun, Yicong Cai, Dahu Zhou, Linjuan Ouyang, Changlan Zhu, Haohua He, Xiaosong Peng
Summary: Introduction of different pest and disease resistance genes is crucial for successful cultivation of transgenic rice strains with broad-spectrum resistance. In this study, resistance rice lines with stacked resistance genes were produced and evaluated for their resistance to multiple pathogens. The findings suggest that stacking of rice resistance genes through molecular marker-assisted backcross breeding can confer broad spectrum and multiple resistance in differently genetic backgrounds.
Review
Microbiology
Jinyi Tan, Haikun Zhao, Josh Li, Yihan Gong, Xin Li
Summary: This article summarizes the key molecular findings on fungal development and pathogenicity mechanisms of Magnaporthe oryzae, focusing on fully characterized genes based on mutant analysis. These genes are involved in various biological processes of this pathogen, such as vegetative growth, conidia development, appressoria formation and penetration, and pathogenicity. In addition, the article also highlights gaps in our current understanding of M. oryzae development and virulence. The hope is that this review will contribute to a comprehensive understanding of M. oryzae and assist in future disease control strategy designs.
Article
Plant Sciences
Dewei Yang, Shengping Li, Yueping Xiao, Ling Lu, Zichao Zheng, Dingzhong Tang, Haitao Cui
Summary: The study highlights the importance of rapid, high-amplitude transcriptional reprogramming in rice defense against blast fungus, with ribosome- and protein translation-related genes being significantly enriched among differentially expressed genes at 12 hpi in both susceptible and resistant cultivars. A core set of genes involved in response to both biotic and abiotic stress was identified, including metallothionein genes positively regulating rice resistance and a peroxidase gene negatively regulating it. This study provides novel insights into transcriptional reprogramming and serves as a valuable resource for functional studies on rice immune signaling components in resistance to blast disease.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Microbiology
Lihong Dong, Yuming Ma, Cheng-Yen Chen, Lizheng Shen, Wenda Sun, Guobing Cui, Naweed I. Naqvi, Yi Zhen Deng
Summary: The rice blast fungus Magnaporthe oryzae produces the phytohormone auxin/IAA, which is crucial for its mycelial growth and pathogenic development. This study identified an indole-3-pyruvic acid (IPA)-dependent IAA synthesis pathway in M. oryzae, consisting of the enzymes tryptophan aminotransferase (MoTam1) and indole-3-pyruvate decarboxylase (MoIpd1).
Article
Plant Sciences
Hao Peng, Michael M. Neff
Summary: In Arabidopsis, two NAC transcription factors, ANAC102 and ATAF1, contribute to the suppression of BAS1 and SOB7 expression along with ATAF2. These three ATAFs directly bind to the promoters of the target genes and may coordinately regulate their expression via protein-protein interactions. Additionally, BRs and white light can transcriptionally suppress the expression of these ATAFs, suggesting a complex regulatory network involving brassinosteroids and light signaling pathways in Arabidopsis.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Microbiology
Ying Zhai, Prabu Gnanasekaran, Hanu R. Pappu
Summary: Through the study of NSs-interacting proteins in tobacco, it was found that NSs preferentially interacts with plant defense-related proteins such as CaM, importin, and HSPs, which may play critical roles in TSWV infection.
Article
Microbiology
Kun Yang, Xiaohua Dong, Jialu Li, Yi Wang, Yang Cheng, Ying Zhai, Xiaobo Li, Lihui Wei, Maofeng Jing, Daolong Dou
Summary: The study identified a total of 25 Necrosis- and Ethylene-inducing peptide 1-like proteins (NLPs) from Pythium oligandrum and Pythium periplocum, with some of them inducing necrosis in plants and showing biocontrol activities. Specifically, PyolNLP5 and PyolNLP7 were found to significantly reduce disease severity and suppress the in planta growth of Phytophthora capsici in solanaceous plants. The Phytophthora suppression effect of PyolNLP5 and PyolNLP7 is linked to the induction of antimicrobial plant defensin genes, which is irrelevant to reactive oxygen species accumulation.
Article
Biochemistry & Molecular Biology
Hao Peng, Jessica Phung, Evan C. Stowe, Amit Dhingra, Michael M. Neff
Summary: ATAF2 plays extensive regulatory roles in pathogenesis, seedling development, and stress responses in Arabidopsis thaliana. The study suggests that ATAF2 promotes ethylene biosynthesis and responses by activating relevant genes, and enhances seedling responses to multiple hormone and stress signals.
Article
Plant Sciences
Ying Zhai, Anirban Roy, Hao Peng, Daniel L. Mullendore, Gurpreet Kaur, Bikash Mandal, Sunil Kumar Mukherjee, Hanu R. Pappu
Summary: This study identifies four silencing suppressors encoded by Croton yellow vein mosaic virus (CYVMV) and its cognate betasatellite, and investigates their subcellular localizations, interaction behavior, and roles in symptom induction and intercellular virus movement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Wei Wei, Liangsheng Xu, Hao Peng, Wenjun Zhu, Kiwamu Tanaka, Jiasen Cheng, Karen A. Sanguinet, George Vandemark, Weidong Chen
Summary: In this study, researchers identified an effector named SsPINE1 that can interact with and inactivate host PGIPs. The presence of SsPINE1 enhances the necrotrophic virulence of Sclerotinia sclerotiorum and reduces the resistance of plants against the fungus.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Kun Yang, Chao Chen, Yi Wang, Jialu Li, Xiaohua Dong, Yang Cheng, Huanxin Zhang, Ying Zhai, Gan Ai, Qingsong Song, Baojian Wang, Wentao Liu, Zhiyuan Yin, Hao Peng, Danyu Shen, Song Fang, Daolong Dou, Maofeng Jing
Summary: Microbial necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) enhance plant resistance against pathogens and activate plant defense. The necrosis-inducing activity of PyolNLP5 is dependent on specific amino acid residues, while plant resistance activation is independent of necrosis and reactive oxygen species accumulation. Non-cytotoxic PyolNLPs also enhance plant resistance and induce plant defense in a cell death-independent manner.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ying Zhai, Bryant Davenport, Keith Schuetz, Hanu R. Pappu
Summary: Potato mop-top virus (PMTV) is a emerging threat to potato production in the United States. A rapid and highly sensitive recombinase polymerase amplification (RPA)-based test was developed for PMTV detection in potato tubers, which can detect as little as 10 fg of PMTV RNA transcript in various potato tissues.
Article
Microbiology
Yang Cheng, Huanxin Zhang, Wenyi Zhu, Qing Li, Rui Meng, Kun Yang, Ziqian Guo, Ying Zhai, Haijing Zhang, Rui Ji, Hao Peng, Daolong Dou, Maofeng Jing
Summary: Ferroptosis is a newly discovered form of cell death characterized by iron accumulation and lipid peroxidation. This study shows that ferroptosis plays a critical role in soybean resistance against Phytophthora sojae infection induced by the biocontrol agent Pythium oligandrum. The findings demonstrate that ferroptosis is an inducible defense mechanism against oomycete infection in soybean.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yifan Zhang, Zhiyuan Yin, Lei Pi, Nan Wang, Jinghao Wang, Hao Peng, Daolong Dou
Summary: In this study, a novel small LRR-RLK regulator NbEIR1 was identified using high-throughput TRV-based gene silencing method, which plays a positive regulatory role in plant immunity by regulating INF1-induced immunity and resistance to P. capsici.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Kun Yang, Yi Wang, Jialu Li, Yaxin Du, Ying Zhai, Dong Liang, Danyu Shen, Rui Ji, Xuexiang Ren, Hao Peng, Maofeng Jing, Daolong Dou
Summary: A non-pathogenic oomycete, Pythium periplocum, produces an elicitin called PpEli2, which can induce plant defense and protect various plants against Phytophthora and fungal infections.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kun Yang, Qiang Yan, Yi Wang, Wenyi Zhu, Xiaodan Wang, Xiaobo Li, Hao Peng, Yang Zhou, Maofeng Jing, Daolong Dou
Summary: A disease control strategy was designed by metabolizing pathogen-derived PI3P with a secreted Arabidopsis thaliana phosphatidylino-sitol-4-phosphate 5-kinase 1 (AtPIP5K1) to enhance resistance against Phytophthora pathogens in soybean and potato plants.
PLANT COMMUNICATIONS
(2023)
Article
Cell Biology
Gan Ai, Jierui Si, Yang Cheng, Rui Meng, Zishan Wu, Ruofei Xu, Xiaodan Wang, Ying Zhai, Hao Peng, Yurong Li, Daolong Dou, Maofeng Jing
Summary: Protein homeostasis is essential for organisms, and BAG co-chaperones play a crucial role in this process. Oomycetes exhibit a unique BAG subfamily with atypical structure and function, forming dimers to carry out their functions. This unconventional BAG provides an exclusive protein homeostasis mechanism in oomycetes.
Article
Biochemistry & Molecular Biology
Zhiyuan Yin, Danyu Shen, Yaning Zhao, Hao Peng, Jinding Liu, Daolong Dou
Summary: Transmembrane kinases (TMKs) play important roles in cellular signaling. The kinase domains of most metazoan and plant TMKs belong to the S/T/Y-kinase superfamily. TMKs in protists have diverse origins and functional diversity. Despite belonging to different kinase subfamilies, protist LRR and GPCR-type TMKs share similar sensory domain architectures with plant and animal TMKs.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Kun Yang, Qiang Yan, Yi Wang, Hao Peng, Maofeng Jing, Daolong Dou
Summary: The study found that soybean polyamine oxidase (GmPAO) can enhance resistance to multiple Phytophthora pathogens without obvious adverse effects on agronomic traits. GmPAO produces H2O2 by oxidizing certain substrates, and the production is specifically promoted during infection by Phytophthora sojae. The research provides a novel approach to enable rapid defense responses in plants upon pathogen infection while minimizing growth penalties under normal conditions.
PHYTOPATHOLOGY RESEARCH
(2022)
