Article
Plant Sciences
Guanglian Liao, Yiqi Li, Hailing Wang, Qing Liu, Min Zhong, Dongfeng Jia, Chunhui Huang, Xiaobiao Xu
Summary: This study investigated the SPS and SUS gene families in Actinidia and found their conserved domains and subgroup distribution. Several genes were found to play important roles in regulating sucrose synthesis and accumulation during fruit growth stages in Actinidia.
Article
Biochemistry & Molecular Biology
Yaoke Duan, Lan Yang, Haijia Zhu, Jie Zhou, Hao Sun, Haijun Gong
Summary: Multiple SPS, SUS, and INV genes were identified in tomato, with differential expression patterns and responses to stress. Subfamily differentiation of SlSPS and SlSUS likely occurred before the split of monocotyledons and dicotyledons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Wajid Ali Khattak, Jiaqi He, Jianfan Sun, Iftikhar Ali, Wasim Bilal, Khalid Ali Zahoor, Khalid Ali Khan, Youhua Wang, Zhiguo Zhou
Summary: This study found that foliar-applied melatonin can improve sugar and nitrogen metabolism enzyme activities in cotton leaves, enhancing their stress tolerance and growth under drought conditions.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Taro Harada, Itsuku Horiguchi, Sayaka Ueyama, Ai Murai, Chie Tsuzuki
Summary: This study illustrates the effects of sucrose treatment and hypoxic treatment on starch content, enzyme activities, and gene expression in carnation petals. The findings reveal how sugar metabolism in carnation petals is regulated in response to environmental cues, with modulation of activities and gene expression of a set of sucrolytic enzymes.
Article
Plant Sciences
Jennifer P. C. To, Ian W. Davis, Matthew S. Marengo, Aabid Shariff, Catherine Baublite, Keith Decker, Rafaelo M. Galvao, Zhihuan Gao, Olivia Haragutchi, Jee W. Jung, Hong Li, Brent O'Brien, Anagha Sant, Tedd D. Elich
Summary: New expression elements were computationally generated for effective transgene expression in crops, showing precise initiation of transcripts, effective splicing of introns, and predictable processing of transcript ends. These elements have the potential to optimize gene expression in future plant biotechnology traits.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lu-Yu Zhou, Shuang Zhang, Li-Yun Li, Guo-Yu Yang, Lei Zeng
Summary: The regulation of gene expression in mammalian cells by combining various cis-regulatory features was studied using expression vectors containing different combinations of regulatory elements. The results showed that protein expression can be regulated by optimizing the combination of cis-acting elements. Additionally, it was found that not all combinations of multiple regulatory elements exhibit synergistic effects to enhance expression further.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Genetics & Heredity
Zhiying Ma, Yan Zhang, Liqiang Wu, Guiyin Zhang, Zhengwen Sun, Zhikun Li, Yafei Jiang, Huifeng Ke, Bin Chen, Zhengwen Liu, Qishen Gu, Zhicheng Wang, Guoning Wang, Jun Yang, Jinhua Wu, Yuanyuan Yan, Chengsheng Meng, Lihua Li, Xiuxin Li, Shaojing Mo, Nan Wu, Limei Ma, Liting Chen, Man Zhang, Aijun Si, Zhanwu Yang, Nan Wang, Lizhu Wu, Dongmei Zhang, Yanru Cui, Jing Cui, Xing Lv, Yang Li, Rongkang Shi, Yihong Duan, Shilin Tian, Xingfen Wang
Summary: The study generated high-quality genomes of two cultivated cotton species and analyzed the distribution of structural variations in different subgenomes, discovering structural variations that influence agronomic traits. The findings provide insights into the role of structural variations in genotype-to-phenotype relationships and their potential utility in crop improvement.
Article
Plant Sciences
Dan Wang, Jietang Zhao, Yaqi Qin, Yonghua Qin, Guibing Hu
Summary: Sucrose synthase (SUS) is a key enzyme in plant sucrose metabolism. In this study, five SUS genes were identified in litchi, sharing high similarity in sequences. These genes were classified into three groups, showing evolutionary conservation. Real-time PCR analysis revealed different expression patterns of these genes in various tissues and developmental stages, indicating their roles in regulating sugar accumulation in litchi aril.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Hongbo Liu, Xiuqin Lin, Xujuan Li, Ziliang Luo, Xin Lu, Qian You, Xiping Yang, Chaohua Xu, Xinlong Liu, Jiayong Liu, Caiwen Wu, Jianping Wang
Summary: In this study, 39 haplotypes of the SPSB gene in sugarcane were identified, some of which were associated with high sucrose synthesis efficiency. The number of haplotypes and the number of haplotypes under positive selection were lower in high sugar content clones. This study provides insights and benefits for molecular assisted breeding of sucrose synthesis in sugarcane in the future.
Article
Biochemistry & Molecular Biology
Jiafang Shen, Yiran Xu, Songli Yuan, Fuxiao Jin, Yi Huang, Haifeng Chen, Zhihui Shan, Zhonglu Yang, Shuilian Chen, Xinan Zhou, Chanjuan Zhang
Summary: In this study, seven genes encoding SPS were identified in the soybean genome. Investigation of these GmSPSs revealed their structural characteristics, phylogenetics, tissue expression patterns, and cold stress response. It was found that the promoters of all GmSPS genes contained multiple low-temperature-responsive elements, and qRT-PCR confirmed that GmSPS8/18 genes were induced by cold treatment in soybean leaves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Mingyi Yang, Qianwei Lin, Zisheng Luo, Zhaojun Ban, Xihong Li, Russel J. Reiter, Shuang Zhang, Lei Wang, Ze Liang, Ming Qi, Li Li
Summary: The protein abundance and metabolic profile in watercored apple were characterized using a combined proteomic and metabolomic approach. The results showed that carbohydrate metabolism, including sucrose and sugar alcohol accumulation, was prioritized enriched in watercored apple. The involvement of calcium signaling in watercore development was implied by the overlap of differently abundant proteins involved in calcium ion binding, starch, and sucrose metabolism. Sucrose synthase protein, involved in calcium ion binding and sugar metabolism, was identified as a potential basis for apple watercore development.
Article
Biochemistry & Molecular Biology
Yoanes Maria Vianney, Klaus Weisz
Summary: The study identified a guanine-rich minisatellite region within the KCNN4 gene that can form a putative G-quadruplex (G4), potentially regulating gene expression. A high-resolution structure analysis revealed that a truncated mutant sequence represents one of several coexisting species of the wild-type sequence, featuring a noncanonical G4 with a V-shaped loop.
Article
Multidisciplinary Sciences
Mengyao Li, Qi He, Ying Huang, Ya Luo, Yong Zhang, Qing Chen, Yan Wang, Yuanxiu Lin, Yunting Zhang, Zejing Liu, Xiao-Rong Wang, Haoru Tang
Summary: Sucrose synthase (SUS) plays a key role in sucrose metabolism and plant development. This study identified and analyzed 14 SUS family genes in Brassica juncea, revealing their conservative structure and functional importance in stem development and stress responses. Phylogenetic analysis grouped the genes into three categories, highlighting their evolutionary relationships.
Article
Biochemistry & Molecular Biology
Aravindan Kalyanasundaram, Brett J. Henry, Cassandra Henry, Ronald J. Kendall
Summary: The study evaluated the potential of bobwhite GAPDH as a reference gene and confirmed high similarity of GAPDH encoding sequences between bobwhite and other avian species. Through various analyses, the study revealed that bobwhite GAPDH is highly similar to GAPDH in other Galliformes in terms of phylogeny, sequence analysis, and protein structure. Additionally, preliminary experiments indicated that GAPDH is a promising candidate for gene expression analysis in bobwhite.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Constantinos Patinios, Sjoerd C. A. Creutzburg, Adini Q. Arifah, Belen Adiego-Perez, Evans A. Gyimah, Colin J. Ingham, Serve W. M. Kengen, John van der Oost, Raymond H. J. Staals
Summary: A simple and widely applicable genome engineering tool SIBR-Cas was developed in this study, utilizing a theophylline-dependent self-splicing intron to control CRISPR-Cas counter-selection and delay editing events. This method was successfully applied to knock-out genes in multiple bacteria species with poor homologous recombination systems, making it suitable for most bacteria. Additionally, SIBR is proposed to have a broader application as a gene expression and gene regulation control mechanism in bacteria.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Yiling Yang, Wenbin Liao, Xiaoling Yu, Bin Wang, Ming Peng, Mengbin Ruan
ACTA PHYSIOLOGIAE PLANTARUM
(2016)
Article
Plant Sciences
Meng-Bin Ruan, Xin Guo, Bin Wang, Yi-Ling Yang, Wen-Qi Li, Xiao-Ling Yu, Peng Zhang, Ming Peng
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Article
Multidisciplinary Sciences
Bin Wang, Xin Guo, Pingjuan Zhao, Mengbin Ruan, Xiaoling Yu, Liangping Zou, Yiling Yang, Xiao Li, Deli Deng, Jixiang Xiao, Yiwei Xiao, Chunji Hu, Xue Wang, Xiaolin Wang, Wenquan Wang, Ming Peng
Article
Plant Sciences
Shuxia Li, Xiang Yu, Zhihao Cheng, Xiaoling Yu, Mengbin Ruan, Wenbin Li, Ming Peng
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Plant Sciences
Xiaoling Yu, Baiming Cui, Mengbin Ruan, Wei Wen, Shuchang Wang, Rong Di, Ming Peng
PLANT GROWTH REGULATION
(2015)
Article
Multidisciplinary Sciences
Wenbin Li, Zehong Ding, Mengbin Ruan, Xiaoling Yu, Ming Peng, Yifei Liu
SCIENTIFIC REPORTS
(2017)
Article
Plant Sciences
Meng-Bin Ruan, Yi-Ling Yang, Kai-Mian Li, Xin Guo, Bin Wang, Xiao-Ling Yu, Ming Peng
Article
Agronomy
Pingjuan Zhao, Xin Guo, Bin Wang, Xiuchun Zhang, Jianbo Sun, Mengbin Ruan, Ming Peng
Summary: Cassava is a drought-tolerant tropical crop, and the transgenic overexpression of MeH1.2 gene in cassava leads to growth inhibition and changes in gene expression related to metabolism and stress response. This study provides valuable insights into the role of MeH1.2 gene in drought resistance of cassava.
Article
Plant Sciences
Meng-Bin Ruan, Xiao-Ling Yu, Xin Guo, Ping-Juan Zhao, Ming Peng
Summary: The study reveals that overexpression of MeGRXC3 in transgenic Arabidopsis enhances stress-related transcription factor genes and its nuclear activity is essential for its function in conferring mannitol sensitivity. Mutation analysis further indicates the importance of conserved motifs for its transactivation activity and functionality in regulating stress-related responses.
Article
Biotechnology & Applied Microbiology
Xin Guo, Xiaoling Yu, Ziyin Xu, Pingjuan Zhao, Liangping Zou, Wenbin Li, Mengting Geng, Peng Zhang, Ming Peng, Mengbin Ruan
Summary: In this study, we identified a drought-responsive CC-type GRX, MeGRXC3, in cassava and demonstrated its role in regulating activity of catalase and stomatal movement under drought stress. Our findings provide insights into the potential application of MeGRXC3 in molecular breeding of cassava crops.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Liangping Zou, Dengfeng Qi, Shuxia Li, Min Zhai, Zhuang Li, Xin Guo, Mengbin Ruan, Xiaoling Yu, Pingjuan Zhao, Wenbin Li, Peng Zhang, Qiuxiang Ma, Ming Peng, Wenbin Liao
Summary: The function of MeNPF4.5, a plasma-membrane-localized gene in cassava, was investigated in this study. MeNPF4.5 acts as an efflux transporter for nitrate under low nitrate conditions, reducing the nitrate efflux capacity, but as an influx transporter under high nitrate conditions, increasing the nitrate absorption. Additionally, MeNPF4.5 negatively regulates osmotic stress and is involved in nitrate flux in cassava seedlings.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Wenbin Liao, Jie Cai, Haixia Xu, Yilin Wang, Yingjie Cao, Mengbin Ruan, Songbi Chen, Ming Peng
Summary: In this study, the transcription factor MebHLH18 was found to play a crucial role in regulating low temperature-induced leaf abscission in cassava. Overexpression of MebHLH18 significantly reduced the rate of leaf abscission, while interference expression increased the rate of leaf abscission. The expression of MebHLH18 was associated with changes in oxidative and antioxidant activities. Genome-wide association studies revealed a relationship between natural variation in the promoter region of MebHLH18 and low temperature-induced leaf abscission. The natural variation led to increased antioxidant levels and slower leaf abscission under low temperatures.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Xiaoling Yu, Xin Guo, Pingjuan Zhao, Shuxia Li, Liangping Zou, Wenbin Li, Ziyin Xu, Ming Peng, Mengbin Ruan
Summary: MeHDZ14 is a transcription factor induced by drought stress, playing an important role in regulating cassava growth. Overexpression of MeHDZ14 leads to dwarfism and downward leaf curling in cassava plants, while inhibiting the morphogenesis of leaf epidermal cells.
Article
Agronomy
Xin Guo, Xiaohui Yu, Chenyu Lin, Pingjuan Zhao, Bin Wang, Liangping Zou, Shuxia Li, Xiaoling Yu, Yinhua Chen, Peng Zhang, Ming Peng, Mengbin Ruan
Summary: Under chilling stress, MeMYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside compared to the wild type. The anthocyanin biosynthesis pathway was more active in the transgenic cassava, and several genes, including MeTT8, were up-regulated. MeMYB2 bound to the MeTT8 promoter and inhibited its expression, thereby blocking anthocyanin accumulation. MeTT8 was shown to bind to the promoter of MeDFR-2 and increased its expression. MeMYB2 acts as an inhibitor of chilling-induced anthocyanin accumulation in cassava.
Article
Agronomy
Bin Wang, Xin Guo, Pingjuan Zhao, Wenbin Liao, Changying Zeng, Kai Li, Yuxun Zhou, Junhua Xiao, Mengbin Ruan, Ming Peng, Yang Bai, Ye Chen
Summary: MeMYB26 is a key gene associated with drought tolerance and biomass storage in cassava, characterized by transcription factor features and multiple critical domains. Variations in its genomic region are significantly correlated with drought resistance traits.
CROP BREEDING AND APPLIED BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yanru Feng, Muhammad Shahedul Alam, Feng Yan, Michael Frei
Summary: Tropospheric ozone has significant effects on the remobilization and allocation efficiency of aboveground biomass and nutrients in cereal crops. Long-term ozone exposure increases straw C:N ratio and affects grain C:N ratio. Grain N concentrations increase significantly under ozone stress, but N yield declines due to grain yield losses. Various indicators of N use efficiency are reduced, indicating reduced N absorption from soil and allocation from vegetative to reproductive organs. Straw C:N ratio is not suitable for predicting wheat productivity. Nitrogen harvest index (NHI) is not affected by ozone stress, but the relationship between harvest index (HI) and NHI is changed by elevated ozone concentration.
Article
Biochemistry & Molecular Biology
Cong Guan, Wei Li, Guoliang Wang, Ruimei Yang, Jinglei Zhang, Jinhong Zhang, Bo Wu, Run Gao, Chunlin Jia
Summary: This study characterized the expression profiles of mRNAs and ncRNAs in switchgrass under drought stress. The up-regulated mRNAs were enriched in starch and sucrose metabolism pathway, while the differentially expressed lncRNAs potentially regulated protein-coding genes. The study also constructed regulatory networks and validated the functionality of the target gene PvSS4 in enhancing drought tolerance.
Article
Biochemistry & Molecular Biology
Juan Nicolas-Espinosa, Lucia Yepes-Molina, Fuensanta Martinez-Bernal, Miriam Fernandez-Pozurama, Micaela Carvajal
Summary: This study aimed to investigate the physiological response of broccoli leaves to abiotic stresses such as salinity and boron toxicity/deficiency. The results showed that the combined stress of salinity and boron deficiency resulted in a significant reduction in plant biomass, and the adaptation mechanisms were associated with water and boron concentration in the leaves. The expression patterns of PIP aquaporins varied among the different stress treatments, and their presence in the plasma membrane and interaction with the lipid environment played potential regulatory roles in facilitating salinity-boron stress adaptation mechanisms.
Review
Biochemistry & Molecular Biology
Wen-Feng Huang, Juan Li, Jian-An Huang, Zhong-Hua Liu, Li-Gui Xiong
Summary: This review examines the seasonal trends of phyllosphere microorganisms in woody and herbaceous plants and explores the factors influencing these trends. While herbaceous and woody plants share some similarities and differences in their phyllosphere microbiomes, further experimental validation is needed.
Article
Biochemistry & Molecular Biology
Changguang Liao, Hui Shen, Zihan Gao, Yunshu Wang, Zhiguo Zhu, Qiaoli Xie, Ting Wu, Guoping Chen, Zongli Hu
Summary: The novel CRF, SlCRF6, plays a crucial role in regulating tomato plant morphology, leaf development, and the accumulation of photosynthetic products.
Article
Biochemistry & Molecular Biology
Alok Madhu, Alok Sharma, Amandeep Kaur, Kashmir Singh, Santosh Kumar Upadhyay
Summary: In this study, 15 TaMDHAR genes were identified in bread wheat and their crucial roles in antioxidants, growth and development, and stress responses were revealed.
Article
Biochemistry & Molecular Biology
Kai Zheng, Yongsheng Cai, Yanying Qu, Lu Teng, Chaoyue Wang, Jie Gao, Quanjia Chen
Summary: In this study, the whole genome identification and bioinformatics analysis of the HCT gene family were performed in G. barbadense. The results showed that the GbHCT114 gene regulates plant trichome development, which is closely related to cotton fiber quality. Gene silencing and overexpression experiments confirmed the important role of GbHCT114 gene in cotton fiber morphology, lignin content, and secondary xylem duct cell wall development. Transcriptomic analysis identified differentially expressed genes associated with lignin synthesis and fiber development.
Review
Biochemistry & Molecular Biology
Tanashvi Seth, Sejal Asija, Shahid Umar, Ravi Gupta
Summary: Plants activate a sophisticated signaling cascade in response to pests and pathogens, with lipids playing a crucial role in mediating these defense responses. Different types of lipids are involved in cell signaling during plant-pathogen interaction and each lipid has specific relevance and contributes to specific signaling cascades. Lipid biosynthetic enzymes, including phospholipases, are involved in the production of defense signaling molecules. Lipids participate in stress signaling by mediating signal transduction, acting as precursors for bioactive molecules, regulating ROS formation, and interacting with phytohormones.
Article
Biochemistry & Molecular Biology
Yangyang Chen, Xiao Wu, Xiaohua Wang, Qionghou Li, Hao Yin, Shaoling Zhang
Summary: 'Nanguo' pears emit a rich aroma when fully ripe, and the important volatile components are the six-carbon compounds derived from the lipoxygenase pathway. This study identified a highly expressed bZIP transcription factor that is induced during the mature stage of 'Nanguo' pears, and demonstrated its regulatory role in fatty acid-derived volatile biosynthesis.
Article
Biochemistry & Molecular Biology
Zhao Geng, Haikuan Dou, Jianguang Liu, Guiyuan Zhao, Linlin Liu, Ning Zhao, Hanshuang Zhang, Yongqiang Wang, Zetong An
Summary: The overexpression of GhFB15 gene decreases the salt tolerance of Arabidopsis plants, while silencing the gene improves the salt tolerance of cotton plants. Furthermore, GhFB15 regulates the accumulation of flavonoids and the levels of ROS.
Article
Biochemistry & Molecular Biology
Linjun Cai, Ancheng Ma, Jiao Lei, Chongsheng He
Summary: METTL4 is identified as a plant DNA 6mA methyltransferase in Arabidopsis thaliana and plays a crucial role in regulating heat stress response.
Article
Biochemistry & Molecular Biology
Zailong Tian, Kun Li, Yaru Sun, Baojun Chen, Zhaoe Pan, Zhenzhen Wang, Baoyin Pang, Shoupu He, Yuchen Miao, Xiongming Du
Summary: Plants have evolved a mechanism called 'stress memory' to survive in various environmental stresses. This study reveals the physiological, biochemical, and molecular mechanisms underlying drought stress memory formation in cotton, highlighting the role of histone modification H3K4me3 in regulating transcriptional memory. It also investigates the intergenerational inheritance of drought stress memory in cotton, providing theoretical guidance for cotton breeding.
