Article
Biotechnology & Applied Microbiology
Mohammad Yazdani, Michelle G. Croen, Tara L. Fish, Theodore W. Thannhauser, Beth A. Ahner
Summary: The overexpression of wild type CrORWT and mutated CrORHis in microalgae significantly increased the accumulation of multiple carotenoids and upregulated the transcription levels of related biosynthetic genes, leading to enhanced tolerance to salt and paraquat stress. Additionally, the endogenous phytohormone abscisic acid (ABA) levels were also elevated in the transgenic cell lines under both normal growth conditions and stress conditions, suggesting a potential role of OR in modulating oxidative stress tolerance.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
Summary: In this study, the accessibility of different isoprenoid precursor pools for sesquiterpenoid production in Chlamydomonas reinhardtii was investigated. It was found that both the cytoplasm and chloroplast can be utilized for the generation of sesquiterpenoids. Targeting the fusion protein to the chloroplast and increasing gene dosage significantly improved sesquiterpene production. In addition, potential prenyl unit transporters were proposed based on bioinformatic analyses, which could enhance the capabilities of sesquiterpenoid production.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Medicinal
Mei-Li Zhao, Wen-Sheng Cai, Si-Qi Zheng, Jia-Lin Zhao, Jun-Liang Zhang, Ying Huang, Zhang-Li Hu, Bin Jia
Summary: This study successfully introduced a novel isopentenol utilization pathway into Chlamydomonas reinhardtii, leading to a significant increase in limonene production. With culture optimization, transgenic algae cultured under a light: dark regimen achieved the highest limonene production of 117μg/L.
Article
Microbiology
Zhao Peng, Gai Liu, Kaiyao Huang
Summary: Research shows that compared to a model organism from the same genus, snow algae exhibit higher growth rates and photosynthetic efficiency in cold environments, indicating better cold tolerance. Snow algae adapt to rapid temperature decrease by efficiently regulating transcription of specific genes to optimize resource assimilation and metabolic pathways. Additionally, horizontally transferred genes critical for cold adaptation in other psychrophiles are also identified in snow algae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Eun Ju Yun, Guo-Chang Zhang, Christine Atkinson, Stephan Lane, Jing-Jing Liu, Donald R. Ort, Yong-Su Jin
Summary: The Chlamydomonas reinhardtii cia5 mutant can produce substantial amounts of glycolate, a high-value cosmetic ingredient, under ambient air conditions. Metabolomic analysis revealed significant differences in metabolic profiles between the cia5 mutant and the wild type strain during the photoautotrophic phase, highlighting the potential for producing glycolate and engineering alternative pathways for glycolate metabolism in C. reinhardtii.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Erika P. Santoro, Ricardo M. Borges, Josh L. Espinoza, Marcelo Freire, Camila S. M. A. Messias, Helena D. M. Villela, Leandro M. Pereira, Caren L. S. Vilela, Joao G. Rosado, Pedro M. Cardoso, Phillipe M. Rosado, Juliana M. Assis, Gustavo A. S. Duarte, Gabriela Perna, Alexandre S. Rosado, Andrew Macrae, Christopher L. Dupont, Karen E. Nelson, Michael J. Sweet, Christian R. Voolstra, Raquel S. Peixoto
Summary: The study demonstrates that Beneficial microorganisms for corals (BMCs) can protect corals from environmental stress, reduce mortality rates, and mitigate coral bleaching and mortality by inducing genetic and metabolic rearrangements in the coral host.
Article
Biology
Felix Proulx-Giraldeau, Jan M. Skotheim, Paul Francois
Summary: Cell size control mechanisms are diverse and influenced by cell cycle structure and selection pressures. The evolution of these mechanisms can lead to the emergence of critical processes in complex systems.
Article
Plant Sciences
Eric Poliner, Andrea W. U. Busch, Linsey Newton, Young Uk Kim, Rachel Clark, Sofia C. Gonzalez-Martinez, Byeong-Ryool Jeong, Beronda L. Montgomery, Eva M. Farre
Summary: The study found that blue light induces the expression of fatty acid desaturase genes in stramenopile microalgae, promoting the synthesis of EPA. The promoters of blue light-induced genes are enriched with E-box-like motifs. Aureochromes, a specific protein in stramenopiles, act as blue light-regulated transcription factors by sensing blue light.
Article
Plant Sciences
Yi-Ying Lee, Rudolph Park, Stephen M. Miller, Yantao Li
Summary: Genetic compensation is proposed as a mechanism to explain phenotypic differences between gene knockouts and knockdowns. This study demonstrates examples of genetic compensation in microalgae and suggests its physiological relevance in TAG biosynthesis under stress. These findings provide guidance for future genetic engineering and mutant characterization efforts.
Article
Biotechnology & Applied Microbiology
Jingxian Zhang, Jiping Shi, Chenyang Yuan, Xiangcen Liu, Guilin Du, Ruimei Fan, Baoguo Zhang
Summary: This study investigated the role of miRNAs in lipid accumulation induced by nitrogen deprivation in Chlamydomonas reinhardtii. It identified a set of miRNAs that showed differential expression after nitrogen deprivation, suggesting their involvement in lipid transport and metabolism. The findings highlight the importance of miRNAs in microalgae lipid accumulation and provide theoretical guidance for bio-oil engineering production.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Raphael Troesch, Fabian Ries, Lisa Desiree Westrich, Yang Gao, Claudia Herkt, Julia Hoppstaedter, Johannes Heck-Roth, Matthieu Mustas, David Scheuring, Yves Choquet, Markus Raeschle, Reimo Zoschke, Felix Willmund
Summary: Exposure to high temperatures causes temporary changes in chloroplast translation, specifically affecting antenna proteins of photosynthesis. This study reveals the sensitive regulatory principles involved in heat acclimation for the biogenesis and repair of chloroplast-encoded core subunits of photosynthetic complexes. The findings suggest that protein biosynthesis and translation elongation play central roles in heat acclimation and are conserved between different plant species.
Article
Biotechnology & Applied Microbiology
M. Plouviez, M. Abyadeh, M. Mirzaei, M. Hasan, J. A. Paulo, B. Guieysse
Summary: Algae can efficiently harvest and store phosphorus as intracellular polyphosphates. Understanding the proteomic response of Chlamydomonas reinhardtii under different phosphorus availability conditions is essential for engineering phosphorus removal and recovery. The study found that Chlamydomonas reinhardtii increases proteins involved in phosphorus assimilation and storage under phosphorus depletion, while increases proteins involved in ribosome structure and synthesis under phosphorus repletion.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Yufei Zhang, Zipeng Gu, Yudong Ren, Lu Wang, Jian Zhang, Chengwei Liang, Shanying Tong, Yitao Wang, Dong Xu, Xiaowen Zhang, Naihao Ye
Summary: The study revealed that Chlamydomonas reinhardtii exhibits specific adaptive evolution to elevated CO2, especially under long-term conditions, where although the nutritional constituents decrease, the growth and photosynthesis of algal cells significantly increase.
MARINE BIOTECHNOLOGY
(2021)
Article
Oncology
Jovanka Gencel-Augusto, Xiaoping Su, Yuan Qi, Elizabeth M. Whitley, Vinod Pant, Shunbin Xiong, Vrutant Shah, Jerome Lin, Encarnacion Perez, Marta L. Fiorotto, Iqbal Mahmud, Abhinav K. Jain, Philip L. Lorenzi, Nicholas E. Navin, Ellen R. Richie, Guillermina Lozano
Summary: Cancer-related alterations of the p53 tetramerization domain (TD) result in monomeric or dimeric p53 states. Monomers are inactive forms of p53, while dimers confer some tumor-suppressive activities through the upregulation of the PPAR pathway. These activities are associated with lower prevalence of thymic lymphomas and increased CD8+ T-cell differentiation.
Article
Plant Sciences
Ningning Zhang, Leila Pazouki, Huong Nguyen, Sigrid Jacobshagen, Brae M. Bigge, Ming Xia, Erin M. Mattoon, Anastasiya Klebanovych, Maria Sorkin, Dmitri A. Nusinow, Prachee Avasthi, Kirk J. Czymmek, Ru Zhang
Summary: In this study, phenotypic comparisons were performed between two commonly used strains of Chlamydomonas, CC-1690 and CC-5325. The results showed that CC-5325 grew faster heterotrophically in the dark, but had lower photosynthetic efficiency and was more heat-sensitive than CC-1690. Additionally, CC-5325 had an intact cell wall with reduced thickness, and could not maintain a sustained circadian rhythm of phototaxis.
Article
Plant Sciences
Khac Nhu Nghi, Andrea Tagliani, Lorenzo Mariotti, Daan A. Weits, Pierdomenico Perata, Chiara Pucciariello
Summary: The availability of auxin in rice coleoptile under submergence correlates with its final length, with the auxin influx carrier AUX1 playing a key role in influencing this trait. The coleoptile tip is involved in determining the final length of rice varieties with long coleoptiles. Experimental results suggest that auxin biosynthesis and transport are underlying factors in the differential elongation between short and long coleoptile-harbouring varieties of japonica rice.
Editorial Material
Plant Sciences
Rashmi Sasidharan, Laurentius A. C. J. Voesenek, Pierdomenico Perata
Article
Multidisciplinary Sciences
Carlos del Cerro, Erika Erickson, Tao Dong, Allison R. Wong, Elizabeth K. Eder, Samuel O. Purvine, Hugh D. Mitchell, Karl K. Weitz, Lye Meng Markillie, Meagan C. Burnet, David W. Hoyt, Rosalie K. Chu, Jan-Fang Cheng, Kelsey J. Ramirez, Rui Katahira, Wei Xiong, Michael E. Himmel, Venkataramanan Subramanian, Jeffrey G. Linger, Davinia Salvachua
Summary: Research shows that two white-rot fungi can utilize lignin as a carbon source and funnel carbon from lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These insights into global carbon cycling and the foundation for a sustainable bioeconomy highlight the importance of simultaneous lignin depolymerization and bioconversion by white-rot fungi.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Florence Mus, Devanshi Khokhani, April M. MacIntyre, Esther Rugoli, Ray Dixon, Jean-Michel Ane, John W. Peters
Summary: This study identifies discrete determinants that lead to ammonium excretion in Azotobacter vinelandii and demonstrates the potential of generating promising biofertilizers capable of transferring fixed nitrogen to crops through gene editing.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Alexander B. Alleman, Florence Mus, John W. Peters
Summary: There is significant interest in promoting biological nitrogen fixation (BNF) to reduce the use of nitrogenous fertilizers in agriculture, but there are still fundamental knowledge gaps. Nitrogenase catalyzes BNF, requiring a large input of ATP and low potential electrons. Organisms that respire aerobically have an advantage in meeting the ATP demands of BNF but face challenges in protecting nitrogenase from inactivation by oxygen.
Review
Plant Sciences
Elena Loreti, Pierdomenico Perata
Summary: Plant miRNAs are small regulatory RNAs that regulate gene expression through gene silencing. Some miRNAs can be exchanged between plants, their pathogens, and parasitic plants. It has been discovered that miRNAs can also be secreted into the external environment and regulate gene expression and phenotype of nearby plants, defining a new concept in plant communication.
Article
Biotechnology & Applied Microbiology
Alexander B. Alleman, Amaya Garcia Costas, Florence Mus, John W. Peters
Summary: This study investigates the mechanisms of low potential electron production during biological nitrogen fixation and reveals the distinct roles of Rnf and Fix in maintaining redox homeostasis.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Plant Sciences
Jian Yang, Iny Elizebeth Mathew, Hormat Rhein, Richard Barker, Qi Guo, Luca Brunello, Elena Loreti, Bronwyn J. Barkla, Simon Gilroy, Pierdomenico Perata, Kendal D. Hirschi
Summary: This study demonstrates that plants lacking a vacuolar H+/Ca transporter are more tolerant to anoxia and submergence. Calcium signals play an important role in anoxia tolerance and could be used to improve the submergence tolerance of crops.
Article
Multidisciplinary Sciences
Hsin-Hua Wu, Michael D. Pun, Courtney E. Wise, Bennett R. Streit, Florence Mus, Anna Berim, William M. Kincannon, Abdullah Islam, Sarah E. Partovi, David R. Gang, Jennifer L. DuBois, Carolyn E. Lubner, Clifford E. Berkman, B. Markus Lange, John W. Peters
Summary: The article introduces the complete CoM biosynthesis pathway in bacteria, revealing distinct chemical steps compared to CoM biosynthesis in methanogenic archaea, which represents a profound instance of convergent evolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Claudia Kiferle, Silvia Gonzali, Sara Beltrami, Marco Martinelli, Katja Hora, Harmen Tjalling Holwerda, Pierdomenico Perata
Summary: This study examines the involvement of iodine in tomato plant nutrition and its potential in salt stress tolerance. The results show that iodine promotes plant growth and fruit yield under normal conditions, and mitigates some negative effects of salt stress, improving plant tolerance to it.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Martina Rovere, Chiara Pucciariello, Claude Castella, Antoine Berger, Marco Forgia, Tran A. A. Guyet, Marc Bosseno, Marie Pacoud, Renaud Brouquisse, Pierdomenico Perata, Alexandre Boscari
Summary: This study revealed that MtERF74 and MtERF75 are important for nitrogen-fixing nodules in legumes, as they regulate the expression of hypoxia-responsive genes and maintain nitrogen fixation activity in nodules.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Alicja B. Kunkowska, Fabrizia Fontana, Federico Betti, Raphael Soeur, Gerold J. M. Beckers, Christian Meyer, Geert De Jaeger, Daan A. Weits, Elena Loreti, Pierdomenico Perata
Summary: Plants respond to oxygen deprivation by activating a set of hypoxia-responsive genes through a group of transcription factors. However, the activity of these factors is greatly reduced when the plant has limited sugar reserves, suggesting the involvement of another sensing pathway related to energy availability. The energy sensor target of rapamycin (TOR) is responsible for the oxygen sensing in plants, and its inhibition leads to a lower induction of hypoxia-responsive genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Eva Maria Gomez-Alvarez, Alessandro Tondelli, Khac Nhu Nghi, Viktoriia Voloboeva, Guido Giordano, Giampiero Vale, Pierdomenico Perata, Chiara Pucciariello
Summary: A genome-wide association study identified a Laccase gene that is crucial for successful seed germination after submergence and recovery in a large barley panel. Flooding events caused by global climate change have a significant impact on crop production. Sensitive barley varieties exhibit underwater secondary dormancy due to lower oxygen permeability, which can be eliminated by nitric oxide donors. The discovery of the differently regulated Laccase gene in grain development provides insights for improving barley genetics and enhancing seed germination after short-term flooding.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Julia S. Martin del Campo, Jack Rigsbee, Marcelo Bueno Batista, Florence Mus, Luis M. Rubio, Oliver Einsle, John W. Peters, Ray Dixon, Dennis R. Dean, Patricia C. Dos Santos
Summary: Understanding the reduction of nitrogen gas to ammonia at ambient conditions has been a longstanding challenge. This knowledge is crucial for transferring biological nitrogen fixation to crops and developing improved synthetic catalysts. Azotobacter vinelandii has emerged as the preferred model organism for studying this process over the past 30 years. This review summarizes the current understanding and places it in historical context.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Review
Plant Sciences
Kurt Fagerstedt, Chiara Pucciariello, Ole Pedersen, Pierdomenico Perata
Summary: Recent progress in researching flooding and hypoxia/anoxia tolerance in plants has revealed individual and general mechanisms of tolerance. This research has investigated carbohydrate consumption, fermentation, aerenchyma development, and the oxygen-sensing mechanism, providing insights into the intracellular effects of flooding. The accumulated knowledge has been applied to breeding flood-tolerant crop cultivars and improving crop productivity under changing environments due to climate change.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)