Article
Agronomy
Pramod S. Shelake, Debabandya Mohapatra, Manoj Kumar Tripathi, Saroj Kumar Giri, Adinath Kate, Manoj Kumar
Summary: This research investigates the efficacy of gaseous ozone for the inactivation of Aspergillus niger and Erwinia carotovora on onion bulbs. The results show that the efficacy of ozone on microorganisms increased with an increase in ozone concentration and the number of exposures. SEM analysis revealed structural disintegration of bacterial cells and fungal spores with the severity of ozone application. Mathematical models were used to describe the survival curves of A. niger and E. carotovora.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shazia Dilbar, Hassan Sher, Dalal Nasser Binjawhar, Ahmad Ali, Iftikhar Ali
Summary: In recent years, there has been increasing interest in the biological synthesis of silver nanoparticles due to their unique properties. This study presents an efficient and eco-friendly method for producing antibacterial silver/silver chloride nanoparticles using leaf extract from Stachys emodi. The synthesized nanoparticles were characterized using various techniques and were found to exhibit strong antibacterial activity against Erwinia carotovora.
Article
Biochemistry & Molecular Biology
Robert Czajkowski, Lukasz Rabalski, Maciej Kosinski, Eigil de Neergaard, Susanne Harding
Summary: Pectobacterium atrosepticum is a plant-pathogenic bacterium causing blackleg of potato worldwide. A high-quality genome of the P. atrosepticum strain Green1 isolated from potato plants in Greenland under a subarctic climate was presented, which provides resources for comparative genomic studies and analyses targeting climatic adaptation and ecological fitness mechanisms.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Multidisciplinary Sciences
Iman Hadizadeh, Bahram Peivastegan, Jinhui Wang, Nina Sipari, Kare Lehmann Nielsen, Minna Pirhonen
Summary: This study used RNA-Seq technology to investigate potato gene expression and hormone production changes during D. solani infection. The results showed that in the early stages of infection, potato activated immune and defense responses, but one week later, primary metabolism was affected and pectic enzymes and cell wall-based defenses were activated. Additionally, there was a high concentration of SA and low concentration of JA in asymptomatic tubers at the beginning of the infection, while there was a high level of JA and reduced level of SA in symptomatic tubers one week later.
Article
Biochemistry & Molecular Biology
Camille Pineau, Natalia Guschinskaya, Isabelle R. Goncalves, Florence Ruaudel, Xavier Robert, Patrice Gouet, Lionel Ballut, Vladimir E. Shevchik
Summary: The type II secretion system transports fully folded proteins through the outer membrane of Gram-negative bacteria, with secretion determinants potentially being of a structural nature. Certain phytopathogenic gamma-proteobacteria secrete similar sets of enzymes via T2SS, but the fine-tuned control of protein recruitment is evident in the secretion of orthologous enzymes by different bacteria. Structural and functional analyses suggest that distant and flexible loop regions play a crucial role in protein secretion, with potential disorder-to-order transitions involved in the recruitment mechanism of T2SS.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Mariana Galvao Ferrarini, Agnes Vallier, Carole Vincent-Monegat, Elisa Dell'Aglio, Benjamin Gillet, Sandrine Hughes, Ophelie Hurtado, Guy Condemine, Anna Zaidman-Remy, Rita Rebollo, Nicolas Parisot, Abdelaziz Heddi
Summary: This study examined the gene expression of both the host and endosymbiont during the life cycle of the cereal weevil. They found a constant arms race between the host and the endosymbiont, involving various biological functions such as immunity, metabolism, metal control, apoptosis, and bacterial stress response.
Article
Multidisciplinary Sciences
Daryna Sokolova, Anna Smolarska, Przemyslaw Bartnik, Lukasz Rabalski, Maciej Kosinski, Magdalena Narajczyk, Dorota M. Krzyzanowska, Magdalena Rajewska, Inez Mruk, Paulina Czaplewska, Sylwia Jafra, Robert Czajkowski
Summary: This study identified two D. solani IPO 2222 mutants, DsR34 and DsR207, that showed reduced ability to macerate potato tuber tissues compared to the wildtype strain. Genome sequencing revealed altered genes encoding secretion protein HlyD and elongation factor Tu, which impacted the proteomes of the mutants.
SCIENTIFIC REPORTS
(2023)
Article
Agronomy
Shikha Tiwari, Umang Goswami, Adinath Kate, Bharat Modhera, Manoj Kumar Tripathi, Debabandya Mohapatra
Summary: The study found that storage conditions and duration can affect the types and quantity of volatile organic compounds (VOCs) generated, with variations in VOCs observed under infection and storage conditions.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Microbiology
Marcin Borowicz, Dorota M. Krzyzanowska, Magdalena Narajczyk, Marta Sobolewska, Magdalena Rajewska, Paulina Czaplewska, Katarzyna Wegrzyn, Robert Czajkowski
Summary: Tailocins are nanomolecular machines produced by bacteria with bactericidal activity. In this study, a new tailocin produced by the plant pathogenic bacterium Dickeya dadantii was characterized. The tailocins showed resistance to pH, temperature, and high osmolarity, and had bactericidal activity against certain strains, but were not toxic to other bacteria and nematodes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Ying Zhang, Hang Yu, Yunfei Xie, Yahui Guo, Yuliang Cheng, Weirong Yao
Summary: In this study, the signal molecules produced by Erwinia carotovora and Pseudomonas fluorescens were identified, and the inhibitory effect of hexanal on signal molecule production was evaluated. The results showed that hexanal can inhibit biofilm formation and extracellular enzyme activity, and this inhibition is achieved by inhibiting signal molecule production.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Chemistry, Inorganic & Nuclear
Zainab Bibi, Mohammad Ali, Mohammed Abohashrh, Imtiaz Ahmad, Haji Khan, Murad Ali, Fazal Akbar, Nisar Ahmad, Arshad Iqbal, Farman Ullah, Zahid Ullah, Tahir Muhmood
Summary: This study focused on the synthesis of stable silver nanoparticles (AgNPs) from Hedera nepalensis extract and their effectiveness in controlling plant pathogenic bacteria. The AgNPs exhibited significant antibacterial activity, with extract-free AgNPs displaying higher inhibition. The study suggests that biogenic nanoparticles from Hedera nepalensis could serve as a promising alternative to synthetic pesticides.
Article
Biochemistry & Molecular Biology
Robert Czajkowski, Lukasz Rabalski, Przemyslaw Bartnik, Sylwia Jafra
Summary: Dickeya solani is an emerging plant-pathogenic bacterium causing disease symptoms in various agriculturally relevant crop species. Most sequenced genomes of D. solani strains come from potato, but this study presents the first complete high-quality genome of D. solani IPO 2019 isolated from an ornamental plant, providing valuable resources for comparative genomic studies.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Multidisciplinary Sciences
Guy Condemine, Bastien Le Derout
Summary: Dickeya are plant pathogenic bacteria that can cause diseases in a wide range of plants. The Out type 2 secretion system (T2SS) is essential for the virulence of Dickeya. Previous studies have shown that the T2SS of D. dadantii secretes various plant cell wall degrading enzymes, including pectinases and cellulases. However, the full range of exoproteins that it can secrete has not yet been fully identified. In this study, we identified four new Out-secreted proteins and demonstrated the importance of two of these proteins in the virulence of D. dadantii.
Article
Microbiology
Cristina Bez, Alfonso Esposito, Hang Dinh Thuy, Minh Nguyen Hong, Giampiero Vale, Danilo Licastro, Iris Bertani, Silvano Piazza, Vittorio Venturi
Summary: This study focused on the pathobiome analysis of rice foot rot disease caused by Dickeya zeae, revealing the impact of the bacterial pathogen on the resident microbiome and the interactions between the pathogen and the microbial community involved in the disease process. The presence of D. zeae was found to alter the resident bacterial community and lead to the formation of microbial consortia associated with the disease state. Additionally, several bacterial species were identified to be co-present with the pathogen, suggesting their potential involvement in the disease process.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yan Chen, Pengfu Liu, Jiequn Wu, Wanqing Yan, Saixue Xie, Xuanrong Sun, Bang-Ce Ye, Xiaohe Chu
Summary: A novel and efficient biocontrol reagent based on N-acylhomoserine lactonase-based hybrid nanoflowers has been developed, which exhibits significant control effect, outstanding environmental adaptability and tolerance.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
