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Identification of Late Blight Resistance-Related Metabolites and Genes in Potato through Nontargeted Metabolomics

PLANT MOLECULAR BIOLOGY REPORTER (2014)

期刊

PLANT MOLECULAR BIOLOGY REPORTER

卷 32, 期 2, 页码 584-595

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SPRINGER

DOI: 10.1007/s11105-013-0665-1

关键词

Metabolomics; Quantitative resistance; Phytophthora infestans; Polygenic resistance; Single-nucleotide polymorphism; Potato

类别

Biochemical Research Methods Plant Sciences

资金

International Development Research Center, Ottawa, Canada
Foreign Affairs, Trade and Development Canada

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摘要

Late blight of potato (Solanum tuberosum) caused by Phytophthora infestans significantly reduces the productivity of potato around the world. Resistance to late blight in potato is either qualitative or quantitative. Qualitative resistance governed by race-specific single R genes is well characterized and gives complete resistance, but is not durable. Quantitative resistance governed by polygenes gives partial resistance, but is durable in nature. However, the quantitative resistance mechanisms are poorly studied and are not efficiently exploited in potato breeding. A nontargeted metabolic profiling of resistant (F06037) and susceptible (Shepody) potato cultivars, using high-resolution liquid chromatography-mass spectrometry, was applied to elucidate the quantitative resistance mechanisms against P. infestans (US-8 genotype). The hydroxycinnamic acid amides (HCAAs) of the shunt phenylpropanoid pathway were highly induced following pathogen inoculation in F06037. In parallel, the transcript abundances of genes that catalyze the biosynthesis of these metabolites, such as 4-coumarate:CoA ligase, tyrosine decarboxylase, ornithine decarboxylase, tyramine hydroxycinnamoyl transferase, and putrescine hydroxycinnamoyl transferase, were also higher in the resistant genotype. Sequencing of the coding genes of these enzymes revealed single-nucleotide polymorphisms between resistant and susceptible genotypes, and the amino acid changes caused missense mutations altering protein functions. HCAAs deposited at host cell walls inhibit pathogen colonization, thus reducing lesion expansion. In addition, these also act as phytoalexins, leading to the reduced biomass of the pathogen. Following validation, the HCAAs can be used as biomarker metabolites for late blight resistance. The putative candidate genes can be either used to develop allele-specific markers for marker-assisted breeding programs or suitably stacked into elite cultivars through cisgenic approaches, following validation.

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Comparative Metabolomic Profiling of Compatible and Incompatible Interactions Between Potato and Phytophthora infestans

Jingyu Zhu, Xue Tang, Yining Sun, Yan Li, Yajie Wang, Yusong Jiang, Huanhuan Shao, Bin Yong, Honghao Li, Xiang Tao

Summary: This study used untargeted metabolomics to investigate the changes in metabolites during the infection process of Phytophthora infestans in potato cultivars. The results showed that metabolic differences mainly emerged after 48 hours postinoculation. Comparisons between the resistant and susceptible cultivars identified metabolites related to resistance, including higher levels of salicylic acid in the resistant cultivar. These metabolites play crucial roles in cell wall thickening and have antibacterial and antifungal activities. This study reveals the metabolomic responses of potatoes to P. infestans and provides insights into the compatible and incompatible interactions between potatoes and P. infestans.

FRONTIERS IN MICROBIOLOGY (2022)