Article
Plant Sciences
Chitose Honsho, Koichiro Ushijima, Misa Anraku, Shuji Ishimura, Qibin Yu, Frederick G. Gmitter, Takuya Tetsumura
Summary: This study identified genes regulating self-incompatibility in Japanese citrus cultivars through RNA-seq, revealing that six T2 RNase genes were S-RNases. The findings support the notion that citrus self-incompatibility is based on S-RNase. Further homology comparisons with S-RNases from Chinese citrus led to the conclusion that three of the T2 RNases were novel citrus S-RNases involved in self-incompatibility.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Koji Kawamura, Yoshihiro Ueda, Shogo Matsumoto, Takanori Horibe, Shungo Otagaki, Li Wang, Guoliang Wang, Laurence Hibrand-Saint Oyant, Fabrice Foucher, Marcus Linde, Thomas Debener
Summary: This study identified the S-locus controlling self-incompatibility in Rosa, and confirmed the genetic linkage between the newly-identified S-RNase S-C2 and the previously-identified S-C1 in chromosome 3. Through breeding history and wild species analysis, it was found that different S-alleles are present in continuous-flowering cultivars, and S-genotyping is helpful for effective breeding.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Hong Zhao, Yanzhai Song, Junhui Li, Yue Zhang, Huaqiu Huang, Qun Li, Yu'e Zhang, Yongbiao Xue
Summary: In this study, the researchers investigated the ubiquitination patterns of Petunia hybrida S-3-RNase and its effects. They found that R II is the major ubiquitination region, removal of which significantly reduces seed sets from cross-pollination. This research reveals the stepwise UPS mechanism that restricts the cytotoxicity of S-RNase in P. hybrida for successful cross-pollination.
Article
Genetics & Heredity
Jie Yu, Baoan Wang, Wenqi Fan, Songbo Fan, Ya Xu, Chunsheng Liu, Tianxing Lv, Wanda Liu, Ling Wu, Linfeng Xian, Tianzhong Li
Summary: The study showed that pollen tube polyamines respond to self-S-RNase and help defend against it, potentially providing a way to break self-incompatibility in apple by enhancing pollen tube tolerance.
Article
Multidisciplinary Sciences
Muhammad Husnain Ahmad, Xin Zheng, Yu Hu, Huimin Liu, Yi Sun, Hao Wen, Lijun Chai
Summary: In this study, a rapid method using agarose gel electrophoresis and aniline blue staining was developed to determine the pollen compatibility of citrus cultivars and observe the growth status of pollen tubes. This method is simple and cost-effective, and it helps in selecting suitable pollinizer trees and parents for breeding programs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Plant Sciences
Lei Wu, Xing Liu, Ming-Yue Zhang, Kai-Jie Qi, Xue-Ting Jiang, Jia-Long Yao, Shao-Ling Zhang, Chao Gu
Summary: Through transcriptome and real-time PCR analyses, two leucine-rich repeat extensin genes, PbLRXA2.1 and PbLRXA2.2, were identified in pear. These genes were found to be significantly upregulated in pollen grains and pollen tubes of the self-compatible cultivar, 'Jinzhui', compared to the self-incompatible cultivar, 'Yali'. PbLRXA2.1 and PbLRXA2.2 promoted pollen tube growth and attenuated the inhibitory effects of self S-RNase, indicating their involvement in the loss of self-incompatibility. The promoters of these two genes were directly bound by the ABRE-binding factor PbABF.D.2, and knockdown of PbABF.D.2 inhibited pollen tube growth. Additionally, self S-RNase repressed the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2, suggesting its role in arresting pollen tube growth by restricting the PbABF.D.2-PbLRXA2.1/PbLRXA2.2 signal cascade.
Article
Plant Sciences
Renata Salcedo-Sanchez, Yuridia Cruz-Zamora, Felipe Cruz-Garcia
Summary: This study identified the regulatory mechanism of the self-incompatibility gene SC10-RNase in tobacco. The presence of a Gypsy element in the SC10-RNase promoter may contribute to DNA methylation remodeling, resulting in abundant expression of SC10-RNase in mature pistils.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Rowan Herridge, Tyler McCourt, Jeanne M. E. Jacobs, Peter Mace, Lynette Brownfield, Richard Macknight
Summary: Self-incompatibility is a characteristic of flowering plants, and grasses have a unique two-locus system to control self-recognition. By analyzing genome data from ryegrass and other grass species, it was found that DUF247 genes and SP/ZP genes are present at the S and Z loci in self-incompatibility species, acting as male and female components. These genes are highly variable, but key secondary structure features are conserved. The time of formation of specificity groups is related to the divergence of grass subfamilies.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Amanda K. Broz, Christopher M. Miller, You Soon Baek, Alejandro Tovar-Mendez, Pablo Geovanny Acosta-Quezada, Tanya Elizabet Riofrio-Cuenca, Douglas B. Rusch, Patricia A. Bedinger
Summary: This study investigated the mechanisms leading to loss of S-RNase function in the Solanaceae family, identifying mutations and transcriptionally silenced genes. The research enriches our understanding of the self-incompatibility system in plants.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Sarah Lee, Felix E. Enciso-Rodriguez, William Behling, Thilani Jayakody, Kaela Panicucci, Daniel Zarka, Satya Swathi Nadakuduti, C. Robin Buell, Norma C. Manrique-Carpintero, David S. Douches
Summary: Using CRISPR-Cas9 gene editing, the S-RNase and HT-B genes were knocked out in diploid self-incompatible potato plants, resulting in self-compatible lines. The study also revealed a synergistic effect between HT-B and S-RNase in self-compatibility. The germplasm generated from this study will be valuable for potato breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chao Tang, Peng Wang, Xiaoxuan Zhu, Kaijie Qi, Zhihua Xie, Hao Zhang, Xiaoqiang Li, Hongru Gao, Tingting Gu, Chao Gu, Shan Li, Barend H. J. de Graaf, Shaoling Zhang, Juyou Wu
Summary: Self-incompatibility (SI) in flowering plants is a genetic system that prevents self-fertilization. In pear, incompatible pollen tubes show swelling at their tips, which is mediated by acetylation of the soluble inorganic pyrophosphatase (PPA) PbrPPA5. Acetylated PbrPPA5 accumulates in the nucleus and forms a transcriptional repression complex with the transcription factor PbrbZIP77 to inhibit the expression of the PME gene PbrPME44.
Article
Agricultural Engineering
Dipnarayan Saha, Subhojit Datta, Arun Kumar Shaw, Maruthi Rangappa Thimmaiah, Pratik Satya, Jiban Mitra, Gouranga Kar
Summary: This study generated a high-quality de novo transcriptome to investigate the LSI in C. juncea and identified a candidate gene, Cjun_RNS3.1, that may be involved in preventing self-fertilization in the male gametophyte.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Chuanbao Wu, Zhaoyu Gu, Tianzhong Li, Jie Yu, Chunsheng Liu, Wenqi Fan, Baoan Wang, Feng Jiang, Qiulei Zhang, Wei Li
Summary: The study demonstrates that in apple pollen tubes, ROS activates downstream genes during S-RNase-mediated gametophytic self-incompatibility, with MdOXI1 phosphorylating MdPTI1L to participate in the signal pathway promoting pollen tube growth.
Editorial Material
Biochemistry & Molecular Biology
Muhammad Jamshed, Neil M. N. Hickerson, Subramanian Sankaranarayanan, Marcus A. Samuel
Summary: Exciting new research reveals the interaction between stigmatic receptors for recognizing self-incompatible pollen and the FERONIA pathway, which regulates the production of stigmatic reactive oxygen species to establish barriers against self-, intra- and interspecific pollen.
Article
Biotechnology & Applied Microbiology
Jianbing Hu, Chenchen Liu, Zezhen Du, Furong Guo, Dan Song, Nan Wang, Zhuangmin Wei, Jingdong Jiang, Zonghong Cao, Chunmei Shi, Siqi Zhang, Chenqiao Zhu, Peng Chen, Robert Larkin, Zongcheng Lin, Qiang Xu, Junli Ye, Xiuxin Deng, Maurice Bosch, Vernonica Franklin-Tong, Lijun Chai
Summary: A new mechanism for self-incompatibility (SI) to self-compatibility (SC) conversion in citrus, which involves the insertion of MITE elements, has been discovered. This study provides the first evidence of the role of MITEs in the S-locus, revealing their importance in the loss of SI phenotype.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biology
Danielle E. Medek, Paul J. Beggs, Bircan Erbas, Alison K. Jaggard, Bradley C. Campbell, Don Vicendese, Fay H. Johnston, Ian Godwin, Alfredo R. Huete, Brett J. Green, Pamela K. Burton, David M. J. S. Bowman, Rewi M. Newnham, Constance H. Katelaris, Simon G. Haberle, Ed Newbigin, Janet M. Davies
Article
Public, Environmental & Occupational Health
Paul J. Beggs, Constance H. Katelaris, Danielle Medek, Fay H. Johnston, Pamela K. Burton, Bradley Campbell, Alison K. Jaggard, Don Vicendese, David M. J. S. Bowman, Ian Godwin, Alfredo R. Huete, Bircan Erbas, Brett J. Green, Rewi M. Newnham, Ed Newbigin, Simon G. Haberle, Janet M. Davies
AUSTRALIAN AND NEW ZEALAND JOURNAL OF PUBLIC HEALTH
(2015)
Letter
Allergy
Bircan Erbas, Shyamali C. Dharmage, Mimi L. K. Tang, Muhammad Akram, Katrina J. Allen, Don Vicendese, Janet M. Davies, Rob J. Hyndman, Ed J. Newbigin, Philip E. Taylor, Philip G. Bardin, Michael J. Abramson
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2015)
Article
Environmental Sciences
Janet M. Davies, Paul J. Beggs, Danielle E. Medek, Rewi M. Newnham, Bircan Erbas, Michel Thibaudon, Connstance H. Katelaris, Simon G. Haberle, Edward J. Newbigin, Alfredo R. Huete
SCIENCE OF THE TOTAL ENVIRONMENT
(2015)
Article
Biochemistry & Molecular Biology
Timothy Paape, Masaomi Hatakeyama, Rie Shimizu-Inatsugi, Teo Cereghetti, Yoshihiko Onda, Tanaka Kenta, Jun Sese, Kentaro K. Shimizu
MOLECULAR BIOLOGY AND EVOLUTION
(2016)
Article
Biochemistry & Molecular Biology
Roman V. Briskine, Timothy Paape, Rie Shimizu-Inatsugi, Tomoaki Nishiyama, Satoru Akama, Jun Sese, Kentaro K. Shimizu
MOLECULAR ECOLOGY RESOURCES
(2017)
Article
Genetics & Heredity
Polina Yu Novikova, Nora Hohmann, Viktoria Nizhynska, Takashi Tsuchimatsu, Jamshaid Ali, Graham Muir, Alessia Guggisberg, Tim Paape, Karl Schmid, Olga M. Fedorenko, Svante Holm, Torbjorn Sall, Christian Schlotterer, Karol Marhold, Alex Widmer, Jun Sese, Kentaro K. Shimizu, Detlef Weigel, Ute Kramer, Marcus A. Koch, Magnus Nordborg
Article
Plant Sciences
Edwin R. Lampugnani, Yin Ying Ho, Isabel E. Moller, Poh-Ling Koh, John F. Golz, Antony Bacic, Ed Newbigin
Article
Allergy
Rachel Tham, Don Vicendese, Shyamali C. Dharmage, Rob J. Hyndman, Ed Newbigin, Emma Lewis, Molly O'Sullivan, Adrian J. Lowe, Philip Taylor, Philip Bardin, Mimi L. K. Tang, Michael J. Abramson, Bircan Erbas
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2017)
Article
Multidisciplinary Sciences
Timothy Paape, Roman Briskine, Gwyneth Halstead-Nussloch, Heidi E. L. Lischer, Rie Shimizu-Inatsugi, Masaomi Hatakeyama, Kenta Tanaka, Tomoaki Nishiyama, Renat Sabirov, Jun Sese, Kentaro K. Shimizu
NATURE COMMUNICATIONS
(2018)
Article
Genetics & Heredity
Timothy Paape, Reiko Akiyama, Teo Cereghetti, Yoshihiko Onda, Akira S. Hirao, Tanaka Kenta, Kentaro K. Shimizu
FRONTIERS IN GENETICS
(2020)
Article
Multidisciplinary Sciences
Sean Walkowiak, Liangliang Gao, Cecile Monat, Georg Haberer, Mulualem T. Kassa, Jemima Brinton, Ricardo H. Ramirez-Gonzalez, Markus C. Kolodziej, Emily Delorean, Dinushika Thambugala, Valentyna Klymiuk, Brook Byrns, Heidrun Gundlach, Venkat Bandi, Jorge Nunez Siri, Kirby Nilsen, Catharine Aquino, Axel Himmelbach, Dario Copetti, Tomohiro Ban, Luca Venturini, Michael Bevan, Bernardo Clavijo, Dal-Hoe Koo, Jennifer Ens, Krystalee Wiebe, Amidou N'Diaye, Allen K. Fritz, Carl Gutwin, Anne Fiebig, Christine Fosker, Bin Xiao Fu, Gonzalo Garcia Accinelli, Keith A. Gardner, Nick Fradgley, Juan Gutierrez-Gonzalez, Gwyneth Halstead-Nussloch, Masaomi Hatakeyama, Chu Shin Koh, Jasline Deek, Alejandro C. Costamagna, Pierre Fobert, Darren Heavens, Hiroyuki Kanamori, Kanako Kawaura, Fuminori Kobayashi, Ksenia Krasileva, Tony Kuo, Neil McKenzie, Kazuki Murata, Yusuke Nabeka, Timothy Paape, Sudharsan Padmarasu, Lawrence Percival-Alwyn, Sateesh Kagale, Uwe Scholz, Jun Sese, Philomin Juliana, Ravi Singh, Rie Shimizu-Inatsugi, David Swarbreck, James Cockram, Hikmet Budak, Toshiaki Tameshige, Tsuyoshi Tanaka, Hiroyuki Tsuji, Jonathan Wright, Jianzhong Wu, Burkhard Steuernagel, Ian Small, Sylvie Cloutier, Gabriel Keeble-Gagnere, Gary Muehlbauer, Josquin Tibbets, Shuhei Nasuda, Joanna Melonek, Pierre J. Hucl, Andrew G. Sharpe, Matthew Clark, Erik Legg, Arvind Bharti, Peter Langridge, Anthony Hall, Cristobal Uauy, Martin Mascher, Simon G. Krattinger, Hirokazu Handa, Kentaro K. Shimizu, Assaf Distelfeld, Ken Chalmers, Beat Keller, Klaus F. X. Mayer, Jesse Poland, Nils Stein, Curt A. McCartney, Manuel Spannagl, Thomas Wicker, Curtis J. Pozniak
Article
Plant Sciences
Timothy Paape, Benjamin Heiniger, Miguel Santo Domingo, Michael R. Clear, M. Mercedes Lucas, Jose J. Pueyo
Summary: Heavy metals are becoming a problem due to contamination from human sources and can enter the food chain through plant uptake. Understanding the genetic basis of metal accumulation and tolerance in plants is important for reducing toxic metal uptake in crops and removing heavy metals from soils using phytoremediation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Gerardo Alvarez-Rivera, Aurora Sanz, Alejandro Cifuentes, Elena Ibanez, Timothy Paape, M. Mercedes Lucas, Jose J. Pueyo
Summary: This study investigates the secondary metabolic changes in response to mercury stress in Medicago truncatula. The results suggest that the synthesis and glycosylation processes of flavonoids play important roles in the tolerance to mercury stress. The accumulation of flavonoids and specific glycosylation patterns are associated with mercury tolerance in different plant varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Geosciences, Multidisciplinary
Kathryn M. Emmerson, Jeremy D. Silver, Edward Newbigin, Edwin R. Lampugnani, Cenk Suphioglu, Alan Wain, Elizabeth Ebert
GEOSCIENTIFIC MODEL DEVELOPMENT
(2019)