Article
Plant Sciences
Shijie Ge, Fugong Ding, Bimpong Daniel, Cuicui Wu, Mingyang Ran, Chi Ma, Yuhang Xue, Die Zhao, Yike Liu, Zhanwang Zhu, Zhengwu Fang, Gaisheng Zhang, Yingxin Zhang, Shuping Wang
Summary: In this study, the relationship between carbohydrate metabolism disorder and pollen abortion in S-type cytoplasmic male sterility (CMS) wheat was investigated. The results showed that the anther and microspore in S-CMS wheat undergo serious structural damage, including premature tapetum degeneration, nutritional shortage, pollen wall defects, and pollen grain malformations. The chloroplasts in the anthers of S-CMS lines also exhibited abnormal carbohydrate metabolism and dysfunction, leading to pollen abortion. Furthermore, key enzymes and genes related to carbohydrate metabolism were significantly inhibited in S-CMS.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Yanru Chen, Yulin Jia, Fuqiang Niu, Yongfeng Wu, Jiali Ye, Xuetong Yang, Lingli Zhang, Xiyue Song
Summary: In this study, a major male fertility restorer locus Rfk1 for Ae. kotschyi cytoplasm in wheat was mapped to chromosome arm 1BS through bulked segregant RNA-Seq analysis. The candidate gene TraesCS1B02G197400LC was identified as the most likely gene associated with fertility restoration. This study provides insights into the mechanism of fertility restoration for K-type CMS in wheat.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Wenlong Yang, Yafei Li, Linhe Sun, Muhammad Shoaib, Jiazhu Sun, Dongzhi Wang, Xin Li, Dongcheng Liu, Kehui Zhan, Aimin Zhang
Summary: The study mapped the gene controlling male sterility in line 15 Fan 03 to a specific region on chromosome 4BS, providing a foundation for marker-assisted selection in wheat hybrid breeding. Three CAPS markers were developed and verified for this purpose, enhancing the utilization of male sterility genetic resources.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Kumar Gaurav, Sanu Arora, Paula Silva, Javier Sanchez-Martin, Richard Horsnell, Liangliang Gao, Gurcharn S. Brar, Victoria Widrig, W. John Raupp, Narinder Singh, Shuangye Wu, Sandip M. Kale, Catherine Chinoy, Paul Nicholson, Jesus Quiroz-Chavez, James Simmonds, Sadiye Hayta, Mark A. Smedley, Wendy Harwood, Suzannah Pearce, David Gilbert, Ngonidzashe Kangara, Catherine Gardener, Macarena Forner-Martinez, Jiaqian Liu, Guotai Yu, Scott A. Boden, Attilio Pascucci, Sreya Ghosh, Amber N. Hafeez, Tom O'Hara, Joshua Waites, Jitender Cheema, Burkhard Steuernagel, Mehran Patpour, Annemarie Fejer Justesen, Shuyu Liu, Jackie C. Rudd, Raz Avni, Amir Sharon, Barbara Steiner, Rizky Pasthika Kirana, Hermann Buerstmayr, Ali A. Mehrabi, Firuza Y. Nasyrova, Noam Chayut, Oadi Matny, Brian J. Steffenson, Nitika Sandhu, Parveen Chhuneja, Evans Lagudah, Ahmed F. Elkot, Simon Tyrrell, Xingdong Bian, Robert P. Davey, Martin Simonsen, Leif Schauser, Vijay K. Tiwari, H. Randy Kutcher, Pierre Hucl, Aili Li, Deng-Cai Liu, Long Mao, Steven Xu, Gina Brown-Guedira, Justin Faris, Jan Dvorak, Ming-Cheng Luo, Ksenia Krasileva, Thomas Lux, Susanne Artmeier, Klaus F. X. Mayer, Cristobal Uauy, Martin Mascher, Alison R. Bentley, Beat Keller, Jesse Poland, Brande B. H. Wulff
Summary: The study sequenced 242 accessions of Aegilops tauschii and identified a rare lineage contributing to the wheat D subgenome. Through association mapping, genomic regions with candidate genes for disease and pest resistance were identified and successfully transferred into wheat. Exploiting the genomic diversity of the wild progenitor allows for rapid trait discovery and functional genetic validation.
NATURE BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Yongqi Li, Meng Zhang, Kashif Shahzad, Yang Han, Xuexian Zhang, Liping Guo, Tingxiang Qi, Huini Tang, Hailin Wang, Xiuqin Qiao, Juanjuan Feng, Zhongxu Lin, Jianyong Wu, Chaozhu Xing
Summary: The deleterious effects of sterile genes or cytoplasms on anther development and main economy traits are hindrances to the large-scale application of three-line hybrids in cotton. This study created four isonuclear-alloplasmic cotton male sterile lines and identified mitochondrial DNA diversities using mitochondrial simple sequence repeat (mtSSR) markers. The mtSSR59 marker effectively classified the CMS-D2, CMS-(AD)1, and CMS-(AD)2 into one category and the CMS-D8 into another category. The marker was also successfully used in marker-assisted selection (MAS) for breeding new male sterile lines and precise differentiation of different CMS-based three-line and conventional cotton hybrids.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Agronomy
Huadong Wang, Qing Xiao, Chao Wei, Hui Chen, Xiaohan Chen, Cheng Dai, Jing Wen, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen, Bin Yi
Summary: By utilizing Brassica 60 K array, bulk segregant analysis, and map-based cloning, a mitochondria-localized pentatricopeptide repeat protein gene was identified and transferred into the hau CMS line, where it successfully restored fertility. The research showed that Rfh was highly expressed in flower buds, and the functional divergence between RFH and rfh may be attributed to 59 amino acid residue differences in the deduced protein sequences.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Fuqiang Niu, Yaning Bu, Xuetong Yang, Yongfeng Wu, Mengting He, Lingli Zhang, Xiyue Song
Summary: This study conducted a fine mapping of the Rfd1 gene in wheat with Aegilops juvenalis cytoplasm and identified a pectinesterase gene, TraesCS1B02G197400LC, which may be responsible for fertility restoration. This research provides a new understanding of the fertility restoration mechanism in cytoplasmic male sterile wheat.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Rongzhi Zhang, Shujuan Zhang, Jihu Li, Jie Gao, Guoqi Song, Wei Li, Shuaifeng Geng, Cheng Liu, Yanxiang Lin, Yulian Li, Genying Li
Summary: Mutations of DCL4, DCL5, and RDR6 were generated using the CRISPR/Cas9 system in wheat to study their effects on reproductive development and phasiRNA production. The study found that RDR6 mutation caused severe consequences throughout plant development, dcl4 mutants exhibited male sterility, and dcl5 plants developed normally but were male sterile. The key genes also evolved differently in wheat, and PHAS genes identified via phasiRNAs diverged rapidly among sub-genomes of polyploid wheat.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Xingxia Geng, Xiaoxia Wang, Jingchen Wang, Xuetong Yang, Lingli Zhang, Xiyue Song
Summary: This study conducted association analysis of protein and transcript expression to screen fertility-related genes using RNA-seq, iTRAQ, and PRM-based assay. The results showed that the gene TaEXPB5 in wheat is upregulated in fertility anthers and is essential for anther or pollen development and male fertility. These findings provide new insights into the molecular mechanism of fertility conversion in thermo-sensitive cytoplasmic male-sterility wheat.
Review
Cell Biology
Koji Murai
Summary: Photoperiod-sensitive cytoplasmic male sterility (PCMS) is proposed as a two-line system for hybrid wheat production in Japan. This system utilizes PCMS lines and a pollinator line to produce hybrid seeds under different photoperiod conditions.
Article
Plant Sciences
Yucui Han, Yiyang Zhang, Guannan Cao, Leilei Shao, Qin Ding, Lingjian Ma
Summary: Thermosensitive cytoplasmic male sterile (TCMS) lines play a crucial role in wheat breeding. MiRNAs can regulate gene expression levels by inhibiting gene translation. YS3038, a wheat TCMS line, was studied for its fertility conversion mechanism through examination of abortion characteristics, miRNA regulation patterns, and target genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Yong-jie Liu, Dan Li, Jie Gong, Yong-bo Wang, Zhao-bo Chen, Bin-shuang Pang, Xian-chao Chen, Jian-gang Gao, Wei-bing Yang, Feng-ting Zhang, Yi-miao Tang, Chang-ping Zhao, Shi-qing Gao
Summary: BS366 is a temperature-sensitive genic male sterile line that is sterile under cold conditions and fertile under normal temperature. Research found abnormalities in meiosis and pollen development in BS366, along with different gene expression and methylation levels under cold conditions. Methylation of genes involved in carbon metabolism or fatty acid metabolism may contribute to male sterility in BS366 spikes.
Article
Plant Sciences
Rahman Ebrahimzadegan, Fatemeh Orooji, Pengtao Ma, Ghader Mirzaghaderi
Summary: This study found that different classes of repetitive DNA sequences have accumulated differently between the strangulata and the other two subspecies of Ae. tauschii, which is generally consistent with spike morphology, suggesting that factors affecting repeatome evolution are variable even among highly closely related lineages.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yucui Han, Yue Zhao, Hairong Wang, Yiyang Zhang, Qin Ding, Lingjian Ma
Summary: This study investigated the mechanism of male sterility in YS3038 by mapping sterile genes and studying the expression of noncoding RNAs. Weighted gene coexpression network analysis and competitive endogenous RNA analysis identified key genes and interactions related to anther development and fertility conversion. Silencing candidate genes led to a decrease in seed setting rate, providing breeders with a new option for developing thermosensitive cytoplasmic male-sterile wheat lines.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Haiyan Yu, Xiaotong Guo, Hailan Cui, Jingchao Chen, Xiangju Li
Summary: The tolerance of Triticum aestivum to mesosulfuron-methyl is higher than that of A. tauschii. The absorption and metabolism of mesosulfuron-methyl are different between the two species. The cytochrome P450 monooxygenase inhibitor, malathion, increases the sensitivity of T. aestivum to mesosulfuron-methyl.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Shivani Sharma, Amit Kumar, Priyanka Dhakte, Gaurav Raturi, Gautam Vishwakarma, Kalyani M. Barbadikar, B. K. Das, S. M. Shivaraj, Humira Sonah, Rupesh Deshmukh
Summary: Crop improvement is a major concern for researchers due to climate change and increasing population. Current progress is not sufficient to meet food demand, hence the need for speed breeding to reduce generation time and enhance efficiency. By combining modern breeding and genetic engineering technologies, speed breeding shows promising potential in achieving nutritional security and sustainable agriculture.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Ruchi Bansal, Surbhi Kumawat, Pallavi Dhiman, Sreeja Sudhakaran, Nitika Rana, Rajdeep Jaswal, Akshay Singh, Humira Sonah, Tilak Raj Sharma, Rupesh Deshmukh
Summary: In this study, we identified eight BAG genes in the rice genome and 78 BAG homologs in nine other Oryza species. We predicted the haplotype diversity and functional effect of variations in BAG genes based on whole genome resequencing data. Transcriptomic analyses revealed the multifunctional roles of BAG genes in both developmental and defense response in rice.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agronomy
Ashish Kumar G. Vala, Nasreen Bano, Yogita Deshmukh, Rukam Singh Tomar, C. G. Joshi, N. Subhash
Summary: This study evaluated the physiological response of 24 rice varieties to salt stress and performed transcriptome analysis. The results showed significant differences in the response to salt stress among different rice varieties, and transcriptome analysis revealed differential gene expression involved in signaling pathways, metabolism, and growth and development.
CEREAL RESEARCH COMMUNICATIONS
(2023)
Article
Plant Sciences
Gaurav Raturi, Surbhi Kumawat, Rushil Mandlik, Deepak Duhan, Vandana Thakral, Sreeja Sudhakaran, Chet Ram, Humira Sonah, Rupesh Deshmukh
Summary: In this study, AQPs in the watermelon genome were identified and analyzed to understand their role and regulation during various abiotic stress conditions. The expression of certain AQPs, particularly ClaPIP1-2 and ClaPIP2-4, was found to be significantly increased under stress. This information provides a fundamental basis for understanding AQP-mediated transport in watermelon and related species.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Biochemistry & Molecular Biology
Surender Singh, Roni Chaudhary, Rupesh Deshmukh, Siddharth Tiwari
Summary: This article summarizes recent advancements in achieving higher homologous recombination based gene targeting efficiency in animals and plants, and discusses strategies and methods to improve homologous recombination efficiency.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Yogesh Sharma, Praveen Soni, Gaurav Raturi, Rushil Mandlik, Vinay Kumar Rachappanavar, Manish Kumar, Prafull Salvi, Durgesh Kumar Tripathi, Hasthi Ram, Rupesh Deshmukh
Summary: Metalloids are elements with properties similar to both metals and non-metals, and their impact on plant growth can be both beneficial and hazardous. This article discusses the specific role and molecular mechanism involved in the uptake and transport of different metalloids in various plant tissues. The efforts to engineer metalloid transport, such as improving the uptake of beneficial metalloids and reducing the presence of hazardous ones, are also described. Understanding the molecular insights into metalloid transport and detoxification mechanisms will aid in utilizing the benefits of metalloids for crop improvement and ensuring food safety.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Cell Biology
Virender Kumar, Vinod Goyal, Rushil Mandlik, Surbhi Kumawat, Sreeja Sudhakaran, Gunashri Padalkar, Nitika Rana, Rupesh Deshmukh, Joy Roy, Tilak Raj Sharma, Humira Sonah
Summary: In this study, a meta-analysis incorporating QTL information, region-specific association, and transcriptomic analysis was performed. The results identified 14 meta-QTLs for oil and 16 meta-QTLs for protein content in soybean. Further analysis using whole genome re-sequenced data revealed a haplotypic association of certain genes with oil and protein content. The transcriptomic analysis provided insights into the tissue-specific expression of candidate genes. This study provides valuable information for map-based cloning of regulatory genes and marker-assisted breeding in soybean.
Review
Plant Sciences
Deepmala Sehgal, Priyanka Dhakate, Heena Ambreen, Khasim Hussain Baji Shaik, Nagenahalli Dharmegowda Rathan, Nayanahalli Munireddy Anusha, Rupesh Deshmukh, Prashant Vikram
Summary: Plant omics has played a crucial role in discovering new genes and biomolecules that can improve crops, and has made significant progress in wheat research on both qualitative and quantitative traits. Combining various omics approaches has led to advances in gene discovery, pathway investigation, and understanding stress responses and yields. The development of a Wheat Omics database further aids functional genomics studies in wheat.
Article
Chemistry, Multidisciplinary
Chintan Kapadia, Rahul Datta, Saiyed Mufti Mahammad, Rukam Singh Tomar, Jasmin Kumar Kheni, Sezai Ercisli
Summary: In this study, miRNAs in Solanum melongena were sequenced using next-generation sequencing platform, and real-time quantitative PCR was used to validate the gene expression patterns of miRNAs in Solanum melongena plantlets infected with Ralstonia solanacearum. The results showed the presence of 375 miRNAs belonging to 29 conserved families, with miR414 being the most abundant family. Some miRNAs showed upregulation in susceptible variety but lower expression in resistant variety after infection, indicating their negative role in the suppression of host immunity.
Article
Plant Sciences
Deo Rashmi, Waquar A. Ansari, Narendra Y. Kadoo, Vitthal T. Barvkar, Rupesh Deshmukh, Altafhusain B. Nadaf
Summary: Pandanus odorifer, a highly salt-tolerant plant species, exhibits mechanisms to regulate ion balance in its roots, stems, and leaves under high salt conditions. Lower levels of silicon, iron, nickel, and titanium, and higher levels of sodium, chloride, magnesium, cadmium, and chromium were found to contribute to its salt tolerance. Additionally, genes related to ion transport and homeostasis were upregulated under high salt conditions, revealing the importance of vacuolar sequestration of sodium and ion distribution in the roots and shoots of P. odorifer for salt stress tolerance.
ACTA PHYSIOLOGIAE PLANTARUM
(2023)
Article
Chemistry, Applied
Gunashri Padalkar, Rushil Mandlik, Sreeja Sudhakaran, Sanskriti Vats, Surbhi Kumawat, Virender Kumar, Vineet Kumar, Anita Rani, Milind B. Ratnaparkhe, Pravin Jadhav, Javaid Akhter Bhat, Rupesh Deshmukh, Tilak Raj Sharma, Humira Sonah
Summary: Soybean is an important crop globally, known for its nutritional value and industrial uses. However, the presence of anti-nutritional factors and off-flavor-producing compounds limits its acceptance as a staple food. Developing anti-nutrient free soybean could help address global malnutrition.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Rajdeep Jaswal, Sivasubramanian Rajarammohan, Himanshu Dubey, Kanti Kiran, Hukam Rawal, Humira Sonah, Rupesh Deshmukh, Tilak Raj Sharma
Summary: A novel effector protein family similar to Kiwellin proteins has been discovered in rust fungi, demonstrating a classical example of effector evolution at the structural level.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Sanskriti Vats, S. M. Shivaraj, Humira Sonah, Gunvant Patil, Joy Roy, Tilak Raj Sharma, Rupesh Deshmukh
Summary: In this study, a highly efficient protocol for tomato transformation was developed and optimized. Different factors affecting regeneration and transformation were evaluated, and the best conditions were determined. This standardized protocol can be applied to transform any tomato variety.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Biochemistry & Molecular Biology
Srutiben A. Gundaraniya, Padma S. Ambalam, Roli Budhwar, Shital M. Padhiyar, Rukam S. Tomar
Summary: RNA sequencing analysis revealed that the drought-tolerant genotype TAG-24 activates genes related to plant hormone signaling pathway and oxidative damage resistance under drought stress, providing valuable resources for understanding drought tolerance mechanism and improving peanut varieties.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Plant Sciences
Hemant B. Kardile, Suhas Gorakh Karkute, Clarissa Challam, Nirmal Kant Sharma, Rahul Mahadev Shelake, Prashant Govindrao Kawar, Virupaksh U. Patil, Rupesh Deshmukh, Vinay Bhardwaj, Kumar Nishant Chourasia, Srikar Duttasai Valluri
Summary: This study identified and characterized 37 putative SWEET genes in potato and investigated their response to the late blight disease caused by Phytophthora infestans. Nine transporter genes were found to be responsive to P. infestans, with their expression levels correlating with the life cycle of the pathogen.
