Article
Cell Biology
Alessandra Roggeri, Guendalina Olivero, Cesare Usai, Tim Vanmierlo, Anna Pittaluga
Summary: This study provides evidence of the existence and distribution of inhibitory S1P1R and facilitatory S1P3R in cortical nerve endings of healthy mice. It also shows that the activity of these receptors is amplified in mice with experimental autoimmune encephalomyelitis (EAE) but can be recovered after chronic FTY720 treatment.
Review
Behavioral Sciences
Maryam Naseh, Jafar Vatanparast, Ali Rafati, Mahnaz Bayat, Masoud Haghani
Summary: FTY720, as an agonist of S1P receptor, exerts neuroprotective effects in ischemic stroke patients through three mechanisms: functional antagonistic, functional agonistic, and receptor-independent mechanisms.
BRAIN AND BEHAVIOR
(2021)
Review
Pharmacology & Pharmacy
Gerhild van Echten-Deckert
Summary: Lipids are essential components of the CNS, and sphingolipids, a type of lipid, are highly concentrated in the brain. Sphingosine 1-phosphate (S1P), derived from membrane sphingolipids, has complex effects on the brain, which can be both beneficial and detrimental. However, the role of S1P in brain development and various brain pathologies is still controversial.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Rachel S. Resop, Bradley Salvatore, Shawn J. Kim, Brent R. Gordon, Bianca Blom, Dimitrios N. Vatakis, Christel H. Uittenbogaart
Summary: HIV-1 infection leads to upregulation of S1P receptor 1 (S1PR1) in the human thymus, potentially increasing the number and speed of thymocyte egress. This may have implications for immune function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Na Wang, Jing-Yi Li, Bo Zeng, Gui-Lan Chen
Summary: Sphingosine-1-phosphate (S1P) is a crucial sphingolipid molecule that regulates cardiovascular functions through binding and activating multiple G protein-coupled receptors in different cell types. It affects cell proliferation, migration, differentiation, and apoptosis through various downstream signaling pathways. Abnormal S1P levels are associated with cardiovascular disorders, and further research is needed to explore the potential therapeutic use of S1P in these diseases.
Article
Multidisciplinary Sciences
Shian Liu, Navid Paknejad, Lan Zhu, Yasuyuki Kihara, Manisha Ray, Jerold Chun, Wei Liu, Richard K. Hite, Xin-Yun Huang
Summary: This study reports the structures and functions of human lysophosphatidic acid (LPA) receptor 1 (LPA(1)) and sphingosine 1-phosphate (S1P) receptor 1 (S1P(1)) in complex with their ligands. The results reveal distinct conformations of LPA and S1P when interacting with their cognate GPCRs, and provide insights into the activation mechanisms of these lipid GPCRs.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Kana Masuda-Kuroki, Shahrzad Alimohammadi, Anna Di Nardo
Summary: Psoriasis is a chronic skin condition that lacks a complete cure. Recent studies have identified sphingolipid metabolites as significant contributors to psoriasis, particularly ceramide and sphingosine-1-phosphate (S1P). The modulation of S1P and its receptor has shown potential in improving psoriasis inflammation.
Article
Cell Biology
Chiara Donati, Francesca Cencetti, Caterina Bernacchioni, Valentina Vannuzzi, Paola Bruni
Summary: Fibrosis is a devastating multifaceted disease that can be treated by regulating the S1P signaling pathway and its cross-talk with other profibrotic mediators.
CELLULAR SIGNALLING
(2021)
Article
Oncology
MargheritaSA Cortini, Andrea Armirotti, Marta Columbaro, Dario Livio Longo, Gemma Di Pompo, Elena Cannas, Alessandra Maresca, Costantino Errani, Alessandra Longhi, Alberto Righi, Valerio Carelli, Nicola Baldini, Sofia Avnet
Summary: This study identified a novel lipid signaling pathway selectively activated in acid-induced metastatic osteosarcoma cells, and demonstrated the impairment of tumor growth by targeting this pathway with FTY720 in combination with a low-serine/glycine diet. The high circulating levels of S1P in osteosarcoma patients suggest its potential as a biomarker.
Article
Pharmacology & Pharmacy
Andrea C. Diaz C. Diaz, Kyle Malone, Jennifer A. Shearer, Anne C. Moore, Christian Waeber
Summary: This study used a rigorous study design to evaluate the translational potential of fingolimod in the treatment of stroke. The results show that the effects of fingolimod vary in different mouse models and may depend on the inflammatory status of the animals.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Steven G. Fagan, Sibylle Bechet, Kumlesh K. Dev
Summary: This study demonstrates that fingolimod may be a potential multimodal therapeutic for Alzheimer's disease, as it can reverse deficits in spatial working memory, alleviate inflammation in the brain, and reduce tau and APP levels in key brain regions.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rachel S. Knipe, Jillian J. Spinney, Elizabeth A. Abe, Clemens K. Probst, Alicia Franklin, Amanda Logue, Francesca Giacona, Matt Drummond, Jason Griffith, Patricia L. Brazee, Lida P. Hariri, Sydney B. Montesi, Katherine E. Black, Timothy Hla, Andrew Kuo, Andreane Cartier, Eric Engelbrecht, Christina Christoffersen, Barry S. Shea, Andrew M. Tager, Benjamin D. Medoff
Summary: Idiopathic pulmonary fibrosis is a chronic disease that causes respiratory failure. This study reveals the importance of vascular permeability in lung repair and fibrosis following injury, and the potential role of lipid mediator S1P in regulating this process.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sonja Michele Schmidtpott, Thorsten Seidel
Summary: This study presents a simple and reliable protocol for FRET measurements, including procedures for protein expression estimation and adjustment of laser and detector settings. Data evaluation will be conducted through calibration with a fluorescent fusion protein of known FRET efficiency.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Chemistry, Medicinal
Ariel L. Burgio, Christopher W. Shrader, Yugesh Kharel, Tao Huang, Joseph M. Salamoun, Kevin R. Lynch, Webster L. Santos
Summary: The S1P1 receptor is targeted by four drugs in the treatment of multiple sclerosis and ulcerative colitis. Targeting Spns2, an S1P exporter, upstream of S1P receptor engagement may provide an alternative strategy to mimic the efficacy of S1P receptor modulators without cardiac toxicity.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Orthopedics
Stefania E. Navone, Rolando Campanella, Laura Guarnaccia, Jean A. Ouellet, Marco Locatelli, Chiara Cordiglieri, Roberta Gualtierotti, Chiara Gaudino, Giuseppe Ciniglio Appiani, Sabino Luzzi, Stefano Borsa, Paolo Rampini, Mauro Pluderi, Lisbet Haglund, Laura Riboni, Mauro Alini, Giovanni Marfia
Summary: The study found that conditioned media from degenerated intervertebral discs activated microglial cells more significantly than those from normal discs, increasing chemotaxis, migration, and release of pro-inflammatory mediators. Additionally, sphingosine-1-phosphate content in the conditioned media from degenerated discs was significantly higher than in normal discs, and FTY720 effectively inhibited the effects of degenerated discs on microglial inflammatory factor transcription and migration.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Youming Zhu, Leu Jin, Ronghua Shi, Jinming Li, Yan Wang, Li Zhang, Chao-Zhao Liang, Vinod K. Narayana, David P. De Souza, Rick F. Thorne, Li Rong Zhang, Xu Dong Zhang, Mian Wu
Summary: This study reveals a novel long noncoding RNA (lncRNA) called gLINC that acts as a backbone for the formation of a metabolon involving glycolytic enzymes and lactate dehydrogenase A. The gLINC metabolon enhances glycolytic flux, ATP production, and cell survival under serine deprivation. Overexpression of gLINC in cancer cells promotes xenograft growth in mice on a serine-deprived diet, suggesting its role in cancer cell adaptation.
Article
Oncology
Sara Alavi, Abdullah Al Emran, Hsin-Yi Tseng, Jessamy C. Tiffen, Helen Marie McGuire, Peter Hersey
Summary: This study explores the role of nicotinamide in preventing and reversing T cell exhaustion, which has important implications for immunotherapy against cancers and viral infections. Nicotinamide supplementation was found to prevent the development of inhibitory receptors in T cells and promote the differentiation of T cells to effector states, suggesting a potential therapeutic strategy for enhancing immune responses.
Article
Engineering, Environmental
Haoshi Gao, Stanislav Kan, Zhuyifan Ye, Yuchen Feng, Lei Jin, Xudong Zhang, Jiayin Deng, Ging Chan, Yuanjia Hu, Yongjun Wang, Dongsheng Cao, Yuanhui Ji, Mingtao Liang, Haifeng Li, Defang Ouyang
Summary: siRNA gene silencing therapy has great potential for treating diseases, but the formulation design of siRNA-LNP faces challenges. A novel integrated computer methodology based on machine learning and molecular dynamic simulation was successfully developed for siRNA-LNP formulation design.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Endocrinology & Metabolism
I-Cheng Lu, Hao Tan, Sheng-Hua Wu, Tzu-Yen Huang, Hsin-Yi Tseng, Jia-Joanna Wang, Gianlorenzo Dionigi, Young Jun Chai, Feng-Yu Chiang, Pi-Ying Chang, Che-Wei Wu
Summary: This study investigated the effect of neostigmine on the signal recovery of electromyography (EMG) following the administration of cisatracurium and rocuronium in a pig model. The results demonstrated that neostigmine accelerated the recovery of EMG signals and improved the amplitude recovery.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Medicine, General & Internal
Feng-Yu Chiang, Ching-Feng Lien, Chih-Chun Wang, Chien-Chung Wang, Tzer-Zen Hwang, Yu-Chen Shih, Hsin-Yi Tseng, Che-Wei Wu, Yaw-Chang Huang, Tzu-Yen Huang
Summary: This article reviews the current literature and proposes standardized FNM procedures during parotid surgery to achieve uniformity, improve application quality, and contribute to future research.
Article
Biochemistry & Molecular Biology
Leixi Cao, Ruijie Wang, Guangzhi Liu, Yuwei Zhang, Rick Francis Thorne, Xu Dong Zhang, Jinming Li, Yang Xia, Lili Guo, Fengmin Shao, Hao Gu, Mian Wu
Summary: This study reveals an interaction between LIF-Stat3 signaling and the glycolytic enzyme Pfkp in the differentiation of murine ESCs into specific cell lineages. The repression of Pfkp by Stat3 maintains pluripotency in ESCs, and lifting this repression results in differentiation towards the ectodermal lineage. Pfkp acts as a protein kinase to phosphorylate Lin41, stabilizing its expression and promoting the binding and degradation of mRNAs encoding ectodermal specification markers.
Article
Biochemical Research Methods
Heather C. Murray, Kasey Miller, Joshua S. Brzozowski, Richard G. S. Kahl, Nathan D. Smith, Sean J. Humphrey, Matthew D. Dun, Nicole M. Verrills
Summary: Acute myeloid leukemia (AML) is a highly aggressive form of leukemia with a poor prognosis. Mutations in kinases, such as FLT3 and KIT, are common in AML patients and are associated with treatment resistance. This study identified DNA-PK as a potential therapeutic target in AML and demonstrated that DNA-PK inhibition sensitizes AML cells with FLT3 and KIT mutations to standard treatments. The findings suggest that targeting DNA-PK could improve the outcomes of AML patients with these mutations.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Education & Educational Research
Ni Zhang, Hsin Yi Tseng, Jasmine Thai, Pranuthi Pagidipati, Chulwoo Park, Nikita Madan, Huda Assaf, Sarah Ahmed, Yoshitaka Iwasaki
Summary: College students faced information overload and misinformation during the early COVID-19 pandemic, highlighting the need for clear and culturally appropriate health communication tailored to their needs. They preferred regular and concise email updates from campus leadership and sought COVID-19 control and prevention information on social media.
JOURNAL OF AMERICAN COLLEGE HEALTH
(2022)
Article
Medicine, General & Internal
Chih-Yun Kuo, Hsin-Yi Tseng, Ivo Stachiv, Chon-Haw Tsai, Yi-Chun Lai, Tomas Nikolai
Summary: Neuropsychological tests are widely used for dementia assessment in clinical practice and are also important for differentiating between Alzheimer's disease (AD) and frontotemporal lobar degeneration (FTLD), especially in the initial clinical presentations of behavioral variants of frontotemporal dementia (bvFTD) and primary progressive aphasia (PPA). However, the overlapping signs and cultural differences raise concerns about the validity and reliability of these tests. This case series combined NPTs with neuroimaging to examine their effectiveness in distinguishing between AD and FTLD, and the results showed that participants diagnosed with FTLD had lower scores in language and social cognition tests compared to AD participants, while PPA participants had lower scores in the Free and Cued Selective Reminding Test compared to bvFTD participants.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Chemistry, Analytical
Szu-Ying Li, Hsin-Yi Tseng, Bo-Wei Chen, Yu-Chun Lo, Huai-Hsuan Shao, Yen-Ting Wu, Ssu-Ju Li, Ching-Wen Chang, Ta-Chung Liu, Fu-Yu Hsieh, Yi Yang, Yan-Bo Lai, Po-Chun Chen, You-Yin Chen
Summary: Increasing requirements for neural implantation have led to advancements in semiconductor technologies, allowing for high-density electrode arrays to improve neural recordings. However, there are technological challenges, such as complex manufacturing processes and environmental concerns. A new eco-fabrication process for neural electrode arrays reduces carbon emissions and offers customization.
Article
Chemistry, Analytical
Yao-Wen Liang, Ming-Liang Lai, Feng-Mao Chiu, Hsin-Yi Tseng, Yu-Chun Lo, Ssu-Ju Li, Ching-Wen Chang, Po-Chuan Chen, You-Yin Chen
Summary: Fiber photometry can overcome methodological limitations and provide new insights into neural systems. It can accurately measure neural activity under deep brain stimulation without artifacts. This study demonstrated the use of a self-assembled optrode as a stimulator and biosensor to record both Ca2+ fluorescence and electrophysiological signals, revealing a correlation between local field potential and Ca2+ fluorescence signal in the evoked region.
Article
Engineering, Multidisciplinary
Na Zhang, Ling Qin, Ping Yu, Wei Gao, Yupeng Li
Summary: With the development of e-commerce and the increase in online shopping, users have higher demands on products. Enterprises need to design products that meet user demands accurately and quickly in order to improve user satisfaction and product competitiveness. This paper proposes a Grey-Markov model of user demands prediction based on online reviews, which predicts the monthly changing trend of user demands in advance. By using LDA topic model and sentiment analysis, user attention values and satisfaction values are obtained. The effectiveness of the proposed method is illustrated through examples of demand prediction and optimization design for smartphones and automobiles, providing a reference for manufacturing enterprises to optimize product design.
JOURNAL OF ENGINEERING DESIGN
(2023)
Review
Medicine, Research & Experimental
Qingjuan Chen, Binhui Yang, Xiaochen Liu, Xu D. Zhang, Lirong Zhang, Tao Liu
Summary: CBP/p300, critical drivers of oncogenesis, promote tumorigenesis and have been shown to be over-expressed in cancer cells; their inhibitors and degraders are considered promising anticancer agents that reduce H3K27ac, regulate oncogene transcription, induce cancer cell death and activate immune response.
Article
Biochemistry & Molecular Biology
Dong Li, Li Na Hu, Si Min Zheng, Ting La, Li Yuan Wei, Xiao Jun Zhang, Zhen Hua Zhang, Jun Xing, Li Wang, Ruo Qi Li, Qin Zhu, Rick F. Thorne, Yu Chen Feng, Hubert Hondermarck, Xu Dong Zhang, Li Li, Jin Nan Gao
Summary: Active crosstalk between the nervous system and breast cancer cells has been experimentally demonstrated. Previous studies may have underestimated the importance of peripheral nerves in breast cancer development and progression. This study found that nerves are commonly present in breast cancers, predominantly of sympathetic origin, and high nerve density is associated with poor patient outcomes.