Article
Agriculture, Multidisciplinary
Ignacio Cabezudo, Carlos A. Lobertti, Eleonora Garcia Vescovi, Ricardo L. E. Furlan
Summary: The PhoP/PhoQ two-component regulatory system plays a crucial role in virulence regulation in Salmonella, but there are limited inhibitors reported so far. This study presents a novel platform that identifies an inhibitor from a pool of compounds, opening up possibilities for the discovery of new bioactive compounds relevant to the food industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Chenxiao Lang, Yimin Zhang, Yanwei Mao, Xiaoyin Yang, Xiaoyun Wang, Xin Luo, Pengcheng Dong, Lixian Zhu
Summary: The study revealed that acid tolerance response (ATR) of Salmonella persists in a low-temperature environment and the PhoP/Q system plays a critical role in ATR. The D value of adapted strains was significantly higher than non-adapted strains, posing a threat to food safety.
Article
Immunology
Suzanne Hower, Ryan McCormack, Sara Schesser Bartra, Patricia Alonso, Eckhard R. Podack, Noula Shembade, Gregory Plano
Summary: Perforin-2, a pore-forming protein critical in defense against bacterial pathogens, is expressed at high levels in certain innate immune cells such as macrophages and neutrophils, but requires induction by interferons or PAMPs in other cell types. Pathogens can limit Perforin-2 expression in inducible cells, while commensal or non-pathogenic bacteria trigger high levels of Perforin-2 expression. Mechanisms for pathogen suppression of Perforin-2 include two-component systems, LPS modification enzymes, and Type III secretion effector proteins.
MICROBIAL PATHOGENESIS
(2021)
Article
Biotechnology & Applied Microbiology
Jing Peng, Zhongnan Xu, Lei Li, Bingjie Zhao, Yanbin Guo
Summary: This study suggests that the PhoP/PhoQ system positively regulates the acid resistance ability of R. aquatilis HX2, indicating its importance in enhancing HX2's adaptation to acidic environments.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Yan Ma, Yingying Zhang, Zhongguo Shan, Xin Wang, Xiaodong Xia
Summary: The study investigated the impact of PhoP/PhoQ two component system on biofilm formation in Cronobacter sakazakii BAA-894. Results showed that the system played crucial roles in regulating gene expression, cell viability, and extracellular polymeric substance content, suggesting it as a potential target for controlling biofilms.
Article
Biotechnology & Applied Microbiology
Yan Ma, Yingying Zhang, Ke Chen, Lingzhu Zhang, Yibei Zhang, Xin Wang, Xiaodong Xia
Summary: The study reveals that the PhoP/PhoQ system plays a crucial role in the growth and survival of C. sakazakii under stress conditions, regulating resistance to environmental stress by affecting multiple gene pathways.
Article
Microbiology
Baopeng Yang, Chang Liu, Xiaolei Pan, Weixin Fu, Zheng Fan, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Summary: The PhoP-PhoQ regulatory system contributes to polymyxin resistance by regulating genes encoding lipopolysaccharide modification enzymes. Additional PhoP-regulated genes were identified through chromatin immunoprecipitation sequencing, revealing their roles in maintaining membrane integrity under stress. Mutations in these genes affected bacterial survival and membrane integrity, highlighting their importance in polymyxin tolerance.
Article
Food Science & Technology
Topi Meuronen, Maria A. Lankinen, Johan Kolmert, Vanessa de Mello Laaksonen, Taisa Sallinen, Jyrki Agren, Kirsi A. Virtanen, Markku Laakso, Craig E. Wheelock, Jussi Pihlajamaki, Ursula Schwab
Summary: This study investigated the interactions between dietary polyunsaturated fatty acids (PUFAs) and variants of fatty acid desaturase (FADS), and found that different genotypes have different effects on plasma lipid fatty acid composition and bioactive lipid mediators. These findings suggest the potential for genotype-based recommendations for PUFA consumption, which can have beneficial effects on health.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Agronomy
Marcella Whetsell, Edward Rayburn
Summary: This study examined the fatty acid content in different types of pasture grasses, legumes, and non-leguminous forbs in northeast West Virginia. The research found variations in fatty acid content among different plant species, with forbs having the highest linoleic acid content and grasses and forbs having the highest alpha-linolenic acid content. The study also evaluated the correlation between crude protein concentration and fatty acid concentration, and found that the summer months and the presence of forbs affected the alpha-linolenic acid content. Vegetative growth and leafiness were identified as major factors influencing the fatty acid content in pasture forage.
Article
Microbiology
Javier F. Mariscotti, Eleonora Garcia Vescovi
Summary: The study reveals that in S. marcescens, the transcriptional regulator RamA is autoregulated and controlled by the PhoP/PhoQ signal transduction system. PhoP is able to directly bind to the promoter regions of ramA, mgtE1, mgtE2, lpxO1, and lpxO2. RamA interacts directly with the promoter regions of ramA and lpxO1 but indirectly regulates mgtE1 and lpxO2.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Plant Sciences
Hongbo Li, Xiaoling Ma, Weiqi Wang, Jiaxi Zhang, Yuanzhe Liu, Deyi Yuan
Summary: The study found that ethephon has a significant impact on the contents of linoleic acid (LA) and alpha-linolenic acid (ALA) in Camellia oleifera seeds, and it can increase their contents by regulating the expression of genes related to LA and ALA metabolism, plant hormone signal transduction pathways, and starch and sucrose metabolism.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
P. Denis, P. Schmidely, P. Noziere, R. Gervais, V Fievez, C. Gerard, A. Ferlay
Summary: The ruminant requirements for essential fatty acids (EFAs), particularly linoleic acid (LA) and alphalinolenic acid (ALA), have not been fully determined. Adequate supply of polyunsaturated fatty acids (FAs) can improve immunity and reproduction in transition cows. Previous studies have predicted EFA intake based on animal characteristics and milk EFA secretions. However, individual variations in EFA digestion and metabolism may affect the accuracy of group-level predictions. This study aimed to establish specific equations for predicting individual cow's EFA intake based on the difference between observed and predicted intake.
Article
Chemistry, Applied
Ana Luiza Fontes, Bruna Neves, Tiago Conde, Daniela Couto, Ligia Leao Pimentel, Luis Miguel Rodriguez-Alcala, M. Rosario Domingues, Ana Maria Gomes
Summary: This study investigated potential fatty acid byproducts during microbial enrichment of CLA/CLNA and found that no additional side-fatty acid metabolites were released except for stearic acid in the presence of linoleic acid.
Article
Agricultural Engineering
Hui Wei, Ali Movahedi, Guoyuan Liu, Sheng Zhu, Yanhong Chen, Chunmei Yu, Fei Zhong, Jian Zhang
Summary: In this study, we identified 36 PtLTPG genes in the poplar genome and divided them into four subgroups. PtLTPG genes were expressed in various tissues of poplar, but with different expression levels. Most PtLTPG and FA-related genes were induced by abiotic stresses, and their expression patterns were associated with each other. Osmotic stress altered the FA composition.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Microbiology
Haoran Guo, Tong Zhao, Can Huang, Jingyu Chen
Summary: This study investigates the role of PhoP/PhoQ in the intrinsic resistance of Yersinia enterocolitica to polymyxin. It is found that PhoP/PhoQ plays an important role in maintaining membrane permeability, intercellular metabolism, and reducing membrane depolarization. LPS modification with structures like L-Ara4N and palmitate decreases the binding ability of polymyxin to Y. enterocolitica. Deletion of PhoP/PhoQ downregulates the transcription of LPS modification genes and PmrA/PmrB-related genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Maria Ayelen Carabajal, Christopher R. M. Asquith, Tuomo Laitinen, Graham J. Tizzard, Lucia Yim, Analia Rial, Jose A. Chabalgoity, William J. Zuercher, Eleonora Garcia Vescovi
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Chemistry, Applied
Ignacio Cabezudo, I. Ayelen Ramallo, Victoria L. Alonso, Ricardo L. E. Furlan
Summary: The study presents a novel platform for discovering tyrosinase inhibitors through compound preparation, biological evaluation, and mass spectrometry identification. The experiments demonstrate the straightforward identification of a new thiosemicarbazone with improved tyrosinase inhibition and anti-browning effects on fresh-cut apple slices compared to kojic acid.
Article
Microbiology
Javier F. Mariscotti, Eleonora Garcia Vescovi
Summary: The study reveals that in S. marcescens, the transcriptional regulator RamA is autoregulated and controlled by the PhoP/PhoQ signal transduction system. PhoP is able to directly bind to the promoter regions of ramA, mgtE1, mgtE2, lpxO1, and lpxO2. RamA interacts directly with the promoter regions of ramA and lpxO1 but indirectly regulates mgtE1 and lpxO2.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Deja Rodgers, Casin Le, Camila Pimentel, Marisel R. Tuttobene, Tomas Subils, Jenny Escalante, Brent Nishimura, Eleonora Garcia Vescovi, Rodrigo Sieira, Robert A. Bonomo, Marcelo E. Tolmasky, Maria Soledad Ramirez
Summary: In the study of Acinetobacter baumannii, a key transcriptional regulator H-NS was found to play an important role in antimicrobial resistance, biofilm production, and quorum sensing gene expression. Inactivation of H-NS led to increased resistance to colistin and altered stress response, while its absence resulted in upregulation of certain genes associated with antibiotic resistance. These findings demonstrate the complexity of the regulatory role of H-NS in combating A. baumannii infections.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Bechara Sina Rahme, Matthieu Lestradet, Gisela Di Venanzio, Arshad Ayyaz, Miriam Wennida Yamba, Martina Lazzaro, Samuel Liegeois, Eleonora Garcia Vescovi, Dominique Ferrandon
Summary: Serratia marcescens is a pathogen that can cause septic injury in Drosophila melanogaster. Through a genetic screen, the flagellum component FliR was identified as an important factor in intestinal infections and damages to the epithelium.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Multidisciplinary
A. Gaston Orrillo, Ricardo L. E. Furlan
Summary: Sulfur plays an important role in dynamic covalent chemistry, especially as part of dynamic sulfur-based bonds (DSBs). DSBs have ideal properties such as high reactivity and good reversibility, which make them highly suitable for the preparation of dynamic covalent systems. They also offer chemical flexibility, opening up multiple applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Organic
Carlos M. Solis, Mario O. Salazar, I Ayelen Ramallo, Paula Garcia, Ricardo L. E. Furlan
Summary: The concomitant selective dehydroxylation during the transformation of flavones into pyrazoles with hydrazine is influenced by the A-ring substitution pattern and propensity to keto enol tautomerization. The obtained pyrazoles exhibit varied tyrosinase inhibition properties, including one nanomolar inhibitor.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Ignacio Cabezudo, Carlos A. Lobertti, Eleonora Garcia Vescovi, Ricardo L. E. Furlan
Summary: The PhoP/PhoQ two-component regulatory system plays a crucial role in virulence regulation in Salmonella, but there are limited inhibitors reported so far. This study presents a novel platform that identifies an inhibitor from a pool of compounds, opening up possibilities for the discovery of new bioactive compounds relevant to the food industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Infectious Diseases
Roberto E. Bruna, Alejo Casal, Barbara Bercovich, Hugo Gramajo, Eduardo Rodriguez, Eleonora Garcia Vescovi
Summary: This study identified and characterized a secreted compound from actinobacteria that inhibits the virulence regulator PhoP in Salmonella, providing a new approach for the development of anti-Salmonella therapeutics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Review
Chemistry, Applied
Ignacio Cabezudo, Mario O. Salazar, I. Ayelen Ramallo, Ricardo L. E. Furlan
Summary: Thin-layer chromatography (TLC) is commonly used for food analysis and quality control. It is compatible with various derivatization methods and can detect compounds related to food stability, contaminants, and nutrition.
Article
Microbiology
Lucila Garcia, Maria Celeste Molina, Kaylie Allyson Padgett-Pagliai, Pablo S. Torres, Roberto E. Bruna, Eleonora Garcia Vescovi, Claudio F. Gonzalez, Jose Gadea, Maria Rosa Marano
Summary: This study identifies a putative protease in the pathogen of Huanglongbing disease, which plays an important role in the virulence of the bacteria. Further experiments demonstrate that this protease regulates the shape, exopolysaccharide production, and biofilm formation of the bacteria, affecting their growth and disease development in plant cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Energy & Fuels
Ignacio Cabezudo, Cecilia S. Galetto, Diana Romanini, Ricardo L. E. Furlan, Maria Rocio Meini
Summary: A biorefinery approach was developed to produce gallic acid using agricultural waste. The best fermentation condition involved the use of soybean hull and grape pomace as support substrate, resulting in efficient conversion of tannic acid to gallic acid.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Pharmacology & Pharmacy
Rosana Rotondo, Pablo Santa Cruz, Marianela Masin, Milagros Burgi, Javier Girardini, Stella M. Garcia, Gustavo R. Rodriguez, Ricardo L. E. Furlan, Andrea M. Escalante
Summary: The aim of this study was to investigate the in vitro chemoprevention and anti-inflammatory properties of three cultivars of artichoke (Gauchito, Guri and Oro Verde). The results showed that leaf extracts from Gauchito and Guri cultivars had inhibitory effects on the proliferation of human neuroblastoma cells and decreased the activity of interferon IFN-I. These findings suggest the potential of these cultivars for the chemoprevention and treatment of neuroblastoma and inflammatory processes.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)