Article
Cell Biology
Wei Chen, Yilong Wang, Wenwen Xia, Jinbao Zhang, Yan Zhao
Summary: This study describes the role of RNA binding protein hnRNPA2B1 in the development of hypertriglyceridemia-induced acute pancreatitis (HTGP). The results show that the level of hnRNPA2B1 protein decreases in PA-treated cells and its expression is regulated by neddylation. hnRNPA2B1 orchestrates fatty acid oxidation by regulating the expression of mitochondrial trifunctional protein-alpha (MTP alpha). Restoration of hnRNPA2B1 expression using the neddylation inhibitor MLN4924 can protect cells from PA-induced inflammatory injury. In vivo administration of MLN4924 restores hnRNPA2B1 protein expression in the pancreas and ameliorates HTGP-associated inflammation and tissue injury.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Rut Fado, Rosalia Rodriguez-Rodriguez, Nuria Casals
Summary: Malonyl-CoA plays a crucial role in regulating various functions in the brain, influencing food intake, energy balance, cognition, and brain development. Recent studies have shown that CPT1 proteins sense and regulate malonyl-CoA, impacting neuronal functions and revealing the effects of nutrients and metabolic disorders on cognition.
PROGRESS IN LIPID RESEARCH
(2021)
Article
Multidisciplinary Sciences
Xiang Shi, Ruiqi Zhang, Zhe Liu, Jun Sun, Lanlan Li, Guiyan Zhao, Junhao Lu
Summary: This study investigated the molecular response of the pacific white shrimp to alkalinity stress using RNA-seq analysis. The results showed significant changes in ion regulation, digestion, and immune response, as well as inhibition of metabolic processes under alkaline stress. These findings provide important insights into the molecular mechanisms of pacific white shrimp under alkalinity stress.
Article
Pharmacology & Pharmacy
Cristina Miralpeix, Ana Cristina Reguera, Anna Fosch, Maria Casas, Jaume Lillo, Gemma Navarro, Rafael Franco, Josefina Casas, Stephen P. H. Alexander, Nuria Casals, Rosalia Rodriguez-Rodriguez
Summary: The study found that CPT1C interacts with ABHD6 and negatively regulates its hydrolase activity, thereby regulating 2-AG downstream signalling. Brain tissues of CPT1C-KO mice showed increased ABHD6 activity. CPT1C malonyl-CoA sensing was key to the regulatory role on ABHD6 activity and CB1 receptor signalling.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shiyun Li, Wenxuan Fu, Ruifang Su, Yunying Zhao, Yu Deng
Summary: In this study, Saccharomyces cerevisiae was engineered to produce malonate, a valuable chemical compound, through genetic mutations and optimization of the production process. The engineered strain showed significantly increased malonate production.
METABOLIC ENGINEERING
(2022)
Article
Cell Biology
Aude Angelini, Pradip K. Saha, Antrix Jain, Sung Yun Jung, Randall L. Mynatt, Xinchun Pi, Liang Xie
Summary: The study reveals that loss of cellular oxygen sensors PHD2/3 inhibits LCFA mitochondrial uptake and beta-oxidation in cardiomyocytes, leading to a metabolic switch. PHD2/3 binds to the key enzyme CPT1B involved in mitochondrial LCFA uptake, promoting its hydroxylation at position P295 and regulation of LCFA metabolism.
Article
Chemistry, Multidisciplinary
Tian Tong, Zhenyan Tao, Xiulai Chen, Cong Gao, Hui Liu, Xiaoling Wang, Gao-Qiang Liu, Liming Liu
Summary: This study established a novel oxaloacetate pathway for efficient 3-HP biosynthesis in yeast through yeast engineering and dephosphorylation regulation strategy, achieving a high-yield production of 3-HP from glucose. It represents a significant advancement in the field of microbial production of platform chemicals.
Article
Biochemistry & Molecular Biology
Haoya Yao, Yaoqing Wang, Xiao Zhang, Ping Li, Lin Shang, Xiaocui Chen, Jia Zeng
Summary: Obesity and diabetes lead to mitochondrial dysfunction and hepatic lipid accumulation, while suppressing fatty acid synthesis and depleting malonyl-CoA in the liver. This study reveals that peroxisomal fatty acid oxidation may play a role in regulating mitochondrial fatty acid oxidation and inducing hepatic steatosis in obese or diabetic animals.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Shiyun Li, Qiyue Zhang, Jing Wang, Yingli Liu, Yunying Zhao, Yu Deng
Summary: In order to reduce reliance on petroleum, utilizing industrial microorganisms for the biosynthesis of important chemicals from simple substrates has become a focus of interest. This review explores strategies to increase intracellular malonyl-CoA levels and advances in metabolic engineering for directing malonyl-CoA to desired derivatives, including strengthening supply, reducing consumption, and controlling levels effectively. These strategies offer new insights for enhancing malonyl-CoA derivative synthesis in microorganisms.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Han She, Lei Tan, Yuanlin Du, Yuanqun Zhou, Ningke Guo, Jun Zhang, Yunxia Du, Yi Wang, Zhengbin Wu, Chunhua Ma, Qinghui Li, Qingxiang Mao, Yi Hu, Liangming Liu, Tao Li
Summary: In this study, a sepsis model was created using cecal ligation and puncture and lipopolysaccharide. The researchers found that VDAC2 lysine malonylation was elevated in sepsis, and that the malonylation of VDAC2 lysine 46 affected mitochondrial-related ferroptosis and myocardial injury. Malonyl-CoA was identified as the primary inducer of VDAC2 malonylation. Inhibition of malonyl-CoA reduced VDAC2 malonylation and alleviated myocardial dysfunction. Targeting VDAC2 malonylation could be a potential treatment strategy for sepsis-induced myocardial dysfunction.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mei-Ling Cheng, Cheng-Hung Yang, Pei-Ting Wu, Yi-Chin Li, Hao-Wei Sun, Gigin Lin, Hung-Yao Ho
Summary: 7-Ketocholesterol (7KCh), the major oxidized product of cholesterol, induces cellular oxidative damage. This study investigated the physiological responses of cardiomyocytes to 7KCh. Treatment with 7KCh inhibited the growth of cardiac cells and their mitochondrial oxygen consumption, while also resulting in compensatory increase in mitochondrial mass and adaptive metabolic remodeling. The accumulation of malonyl-CoA was found to play a role in the growth inhibitory effect of 7KCh.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Makoto Honda, Yosuke Shigematsu, Mihoko Shimada, Yoshiko Honda, Katsushi Tokunaga, Taku Miyagawa
Summary: This study provides evidence that abnormal fatty acid metabolism is involved in the pathophysiology of narcolepsy type 1 (NT1) and other hypersomnia, shedding new light on the understanding of the underlying mechanisms of these disorders.
Article
Biotechnology & Applied Microbiology
Lars Glaser, Martin Kuhl, Julian Stegmuller, Christian Ruckert, Maksym Myronovskyi, Jorn Kalinowski, Andriy Luzhetskyy, Christoph Wittmann
Summary: Supplementation with l-valine significantly increased pamamycin production and elevated the fractions of larger derivatives in the recombinant producer S. albus J1074/R2. However, l-valine negatively impacted strain growth, repressed genes related to mannitol uptake and glycolysis, and hindered pamamycin formation despite transcriptional activation of the biosynthetic gene cluster.
MICROBIAL CELL FACTORIES
(2021)
Review
Microbiology
John E. Cronan
Summary: This review provides an update on the research of Escherichia coli acetyl-CoA carboxylase (ACC), focusing on the structures, stoichiometry, regulation of ACC activity and expression.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Laura E. Parra Daza, Lina Suarez Medina, Albert E. Tafur Rangel, Miguel Fernandez-Nino, Luis Alberto Mejia-Manzano, Jose Gonzalez-Valdez, Luis H. Reyes, Andres Fernando Gonzalez Barrios
Summary: The molecule (2S)-naringenin is currently produced through chemical synthesis and plant isolation, but heterologous biosynthesis is emerging as a viable alternative. This study designed and assembled a bio-factory for (2S)-naringenin production, utilizing specific genes for the biosynthetic pathway. The effect of oxygen transfer on (2S)-naringenin production was evaluated, and the highest titer was obtained at bench scale.
Review
Pharmacology & Pharmacy
Helena Muley, Rut Fado, Rosalia Rodriguez-Rodriguez, Nuria Casals
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mineia Weber, Paula Mera, Josefina Casas, Javier Salvador, Amaia Rodriguez, Sergio Alonso, David Sebastian, M. Carmen Soler-Vazquez, Carla Montironi, Sandra Recalde, Raquel Fucho, Maria Calderon-Dominguez, Joan Francesc Mir, Ramon Bartrons, Joan Carles Escola-Gil, David Sanchez-Infantes, Antonio Zorzano, Vicenta Llorente-Cortes, Nuria Casals, Victor Valenti, Gema Fruhbeck, Laura Herrero, Dolors Serra
Review
Biotechnology & Applied Microbiology
Jesus Garcia-Chica, West Kristian D. Paraiso, Shihori Tanabe, Dolors Serra, Laura Herrero, Nuria Casals, Jordi Garcia, Xavier Ariza, Sabina Quader, Rosalia Rodriguez-Rodriguez
Article
Pharmacology & Pharmacy
Cristina Miralpeix, Ana Cristina Reguera, Anna Fosch, Maria Casas, Jaume Lillo, Gemma Navarro, Rafael Franco, Josefina Casas, Stephen P. H. Alexander, Nuria Casals, Rosalia Rodriguez-Rodriguez
Summary: The study found that CPT1C interacts with ABHD6 and negatively regulates its hydrolase activity, thereby regulating 2-AG downstream signalling. Brain tissues of CPT1C-KO mice showed increased ABHD6 activity. CPT1C malonyl-CoA sensing was key to the regulatory role on ABHD6 activity and CB1 receptor signalling.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rut Fado, Rosalia Rodriguez-Rodriguez, Nuria Casals
Summary: Malonyl-CoA plays a crucial role in regulating various functions in the brain, influencing food intake, energy balance, cognition, and brain development. Recent studies have shown that CPT1 proteins sense and regulate malonyl-CoA, impacting neuronal functions and revealing the effects of nutrients and metabolic disorders on cognition.
PROGRESS IN LIPID RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jaume Lillo, Alejandro Lillo, David A. Zafra, Cristina Miralpeix, Rafael Rivas-Santisteban, Nuria Casals, Gemma Navarro, Rafael Franco
Summary: The study investigated the functional and molecular interactions between ghrelin GHSR1a and cannabinoid CB2 receptors at the CNS level, identifying the presence of CB2-GHSR1a receptor complexes with impaired signaling in a heterologous system. Interestingly, an upregulation of these complexes was found in striatal neurons from offspring of high-fat-diet mouse mothers, indicating a potential link to obesity risk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anna Fosch, Sebastian Zagmutt, Nuria Casals, Rosalia Rodriguez-Rodriguez
Summary: SF1 neurons in the ventromedial hypothalamus play a crucial role in sensing glucose levels and mediating insulin and leptin signaling, as well as in energy expenditure and glucose homeostasis, particularly in the early stages of obesity. While studies have provided insight into the central role of these neurons in whole-body energy homeostasis, they have also raised new questions regarding the existence of neuronal sub-populations and the complexity of neurocircuitry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Cristina Miralpeix, Ana Cristina Reguera, Anna Fosch, Sebastian Zagmutt, Nuria Casals, Daniela Cota, Rosalia Rodriguez-Rodriguez
Summary: The hypothalamus plays a crucial role in the pathogenesis of obesity, with disrupted endocannabinoid system considered a landmark indicator. Recent research has expanded knowledge of the complex endocannabinoid system, focusing on CB receptors, ligands, non-CB receptors, and metabolizing enzymes, particularly in the hypothalamus. The insights gained have provided promising targets for tackling obesity and related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Nutrition & Dietetics
Rut Fado, Anna Molins, Rocio Rojas, Nuria Casals
Summary: In recent years, traditional eating habits have been replaced by a more globalized diet rich in saturated fatty acids and simple sugars. Extensive evidence shows that this diet contributes to cognitive health impairment and increases the incidence of metabolic diseases such as obesity and diabetes. However, the mechanisms through which these nutrients modulate synaptic function and neuroplasticity are not well understood. This review focuses on the effects of Western, ketogenic, and paleolithic diets on cognition and their correlations with synaptic changes, primarily in animal models, to understand the molecular alterations that may contribute to impaired human cognition.
Article
Biochemistry & Molecular Biology
Anna Fosch, Maria Rodriguez-Garcia, Cristina Miralpeix, Sebastian Zagmutt, Maite Larranaga, Ana Cristina Reguera, Jesus Garcia-Chica, Laura Herrero, Dolors Serra, Nuria Casals, Rosalia Rodriguez-Rodriguez
Summary: Sensing of long-chain fatty acids (LCFA) in the hypothalamus modulates energy balance and disruption leads to obesity. The effects of saturated or unsaturated LCFA on hypothalamic-brown adipose tissue (BAT) axis and underlying mechanisms were investigated. Unsaturated LCFA increased BAT thermogenesis through CPT1C-mediated FA metabolism shift, while saturated LCFA did not. These findings provide new insight into neuronal circuitries activated by LCFA to drive thermogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Helena Muley, Karmele Valencia, Josefina Casas, Bea Moreno, Luis Botella, Fernando Lecanda, Rut Fado, Nuria Casals
Summary: Breast cancer is the most common malignancy in women worldwide, and chemoresistance is a major obstacle to patient survival. CPT1C expression has been found to be associated with plasma membrane remodelling and drug resistance in breast cancer cells, suggesting its potential as a predictive biomarker for breast cancer chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Rut Fado, Sebastian Zagmutt, Laura Herrero, Helena Muley, Rosalia Rodriguez-Rodriguez, Huichang Bi, Dolors Serra, Nuria Casals
Summary: There is a need for reliable genetic biomarkers in the diagnosis, prognosis, and treatment of different tumor types. One potential biomarker is CPT1C, a neuronal protein involved in cancer cell adaptation to nutrient depletion and hypoxia. However, its molecular role in cancer cells is controversial. This review discusses the evidence on CPT1C in neurons and its potential role in lipid metabolism and cell adaptation to stressors in cancer cells.
CELL DEATH & DISEASE
(2023)
Editorial Material
Neurosciences
Alice Louail, Iker Bengoetxea de Tena, Rocio Rojas, Nuria Casals
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Article
Neurosciences
Guillermo Iborra-Lazaro, Souhail Djebari, Irene Sanchez-Rodriguez, Esther Gratacos-Batlle, Nuria Sanchez-Fernandez, Marija Radosevic, Nuria Casals, Juan de Dios Navarro-Lopez, David Soto del Cerro, Lydia Jimenez-Diaz
Summary: CPT1C is a neuron-specific protein that is widely distributed in the CNS and expressed in various brain regions. Its deficiency has been shown to disrupt dendritic spine maturation and AMPA receptor synthesis and trafficking, affecting synaptic plasticity and cognitive processes.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Materials Science, Biomaterials
West Kristian D. Paraiso, Jesus Garcia-Chica, Xavier Ariza, Sebastian Zagmutt, Shigeto Fukushima, Jordi Garcia, Yuki Mochida, Dolors Serra, Laura Herrero, Hiroaki Kinoh, Nuria Casals, Kazunori Kataoka, Rosalia Rodriguez-Rodriguez, Sabina Quader
Summary: The study demonstrates the use of PIC micelles as a promising delivery platform for specific CPT1A inhibitors in glioma cells and neurons, resulting in a significant reduction in ATP synthesis and subsequent inhibition of fatty acid oxidation, with a lowered IC50 after long-term incubation.
BIOMATERIALS SCIENCE
(2021)
Article
Psychology, Biological
Umit Yilmaz, Kevser Tanbek
Summary: This study investigated the effects of intracerebroventricular administration of Spexin on the hypothalamus-pituitary-thyroid axis and energy expenditure in rats. The results showed that Spexin reduced food consumption and body weight, increased thyroid hormones, and enhanced energy metabolism.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Kuei-Yu Chien, Yun-Ju Chen, Kuo-Jen Hsu, Chiao-Nan Chen
Summary: This study investigated the effects of a high-protein diet and high-intensity interval training on appetite and weight loss in obese middle-aged individuals. The results showed that consuming a high-protein drink and following a high-protein diet after exercise can reduce post-exercise appetite and the frequency of late-night snacking.
PHYSIOLOGY & BEHAVIOR
(2024)
Review
Psychology, Biological
Melissa Alves Braga Oliveira, Ana Carolina Odebrecht Vergne de Abreu, Debora Barroggi Constantino, Andre C. Tonon, Antoni Diez-Noguera, Fernanda Gaspar Amaral, Maria Paz Hidalgo
Summary: Biological processes in living organisms exhibit strong rhythmicity and are regulated by internal timing systems. Understanding the influence of biological rhythms is crucial for experimental design and reporting.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Yanqun Cao, Hao Chen, Yinna Tan, Xu-Dong Yu, Chuli Xiao, Yin Li, James Reilly, Zhiming He, Xinhua Shu
Summary: There is evidence to suggest that chronic stress impacts neurochemical homeostasis and contributes to mental disorders. This study investigates the therapeutic potential of p-coumaric acid (p-CA), a natural compound found in vegetables and fruits, against stress-associated mental disorders. The findings suggest that p-CA could alleviate cognitive deficits and depression-like behavior in mice exposed to chronic restraint stress (CRS) by regulating the PKA-CREB-BDNF signaling pathway.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Mai O. Spaulding, Jessica R. Hoffman, Grace C. Madu, Magen N. Lord, Caroline Soares Iizuka, Kevin P. Myers, Emily E. Noble
Summary: Food insecurity is associated with obesity and disordered eating behaviors. Studying a rodent model, researchers found that adolescent food insecurity may increase susceptibility to obesity and altered eating behaviors during adulthood.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
K. Oberman, B. L. van Leeuwen, M. Nabben, J. E. Villafranca, R. G. Schoemaker
Summary: The present study investigated the post-operative complications and therapeutic potential of J147 in male Zucker rats, and found that J147 treatment had positive effects on behavioral and metabolic parameters, but did not affect neuroinflammation. The results suggest that a combination of acute and chronic J147 treatment may be optimal for treatment.
PHYSIOLOGY & BEHAVIOR
(2024)
Review
Psychology, Biological
Mathieu Cournoyer, Alice Maldera, Alexandre-Charles Gauthier, Fabien Dal Maso, Marie-Eve Mathieu
Summary: This article provides a comprehensive view of the literature on the effect of different odors on physical activity through a systematic review. It was found that pleasant odors have a positive impact on participants' physical activity. However, better methodological consistency is needed in studies to produce more meaningful results.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Gabriel R. Gilmore, Jeff Dyche
Summary: This study examined sleep, sleep/wake regularity, and cognition in college students diagnosed with depression and using serotonergic antidepressants, comparing them to those without a depression diagnosis. The results showed that students using antidepressants had slightly longer wake after sleep onset and lower sleep efficiency, but these differences were likely not noticed by the participants. There were no differences in sleep regularity or cognition between the two groups.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Erica A. Cross, Kim L. Huhman, H. Elliott Albers
Summary: Social stress plays a significant role in the development of neuropsychiatric disorders and can lead to behavioral deficits such as social withdrawal. This study investigates the impact of social stress on social reward in Syrian hamsters. The results show that subordinate and socially defeated males have reduced motivation for social interactions compared to dominant males. Additionally, winning males exhibit greater activation in the mesolimbic dopamine system compared to losers. In females, there were no differences in social entries between winners and losers, but winning females display more activation in the NAc shell.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Daniel A. R. Cabral, Maria L. M. Rego, Eduardo B. Fontes, Vagner D. O. Tavares
Summary: This study examined the association between body mass index (BMI) and negative emotional states (NES) in men with substance use disorders (SUD) undergoing treatment. The findings showed a positive correlation between BMI and stress, anxiety, and depression. These results suggest that reducing body fat accumulation may contribute to improving mental health in individuals with SUD during recovery.
PHYSIOLOGY & BEHAVIOR
(2024)
Review
Psychology, Biological
Elizabeth Agbor Epse Muluh, Jessica C. McCormack, Yunfan Mo, Michael Garratt, Mei Peng
Summary: This PROSPERO pre-registered systematic review and meta-analysis evaluated the olfactory and gustatory changes in pregnant individuals. The meta-analysis revealed that pregnant individuals performed poorer in odour identification, rated olfactory stimuli to be more intense during the second and third trimester, and had increased pleasantness for sweet taste in the first trimester. No major difference was observed in terms of gustatory functions between pregnant and non-pregnant subjects.
PHYSIOLOGY & BEHAVIOR
(2024)
Article
Psychology, Biological
Renee Spiteri Douglas, Mackenzie R. Hartley, J. Renee Yang, Tamara B. Franklin
Summary: The expression of Hdac2 in the hippocampus is associated with social status, while the expression of closely related genes Hdac1 and HDAC2 protein is not associated with social rank in the hippocampus.
PHYSIOLOGY & BEHAVIOR
(2024)