Article
Acoustics
Ziga Pandur, Matevz Dular, Rok Kostanjsek, David Stopar
Summary: This study systematically investigated the resistance of bacterial cells to ultrasonic irradiation by altering the material properties of E. coli cells. The results showed that peptidoglycan, a major component of the cell wall, was the most important determinant of bacterial resistance to sonolysis. Cells without peptidoglycan exhibited high sensitivity to sonolysis.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Environmental Sciences
Cristiane Thompson, Leonardo Bacha, Pedro Henrique C. Paz, Marcelo de Assis Passos Oliveira, Braulio Cherene Vaz Oliveira, Claudia Omachi, Caroline Chueke, Marcela de Lima Hilario, Michele Lima, Luciana Leomil, Thais Felix-Cordeiro, Thalya Lou Cordeiro da Cruz, Koko Otsuki, Livia Vidal, Mateus Thompson, Renan Ribeiro e Silva, Carlos Mauricio Vicuna Cabezas, Bruno Marque Verissimo, Jose Luiz Zaganelli, Ana Caroline N. Botelho, Lucia Teixeira, Carlos Cosenza, Paulo Marcio Costa, Felipe Landuci, Diogo A. Tschoeke, Tatiana Araujo Silva, Marcia Attias, Wanderley de Souza, Carlos E. de Rezende, Fabiano Thompson
Summary: This study analyzed the problems of scallop aquaculture in southeast Brazil and found that global warming and declining seawater quality are the main causes of the collapse of the industry.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
John L. Robertson, Ryan S. Senger, Janine Talty, Pang Du, Amr Sayed-Issa, Maggie L. Avellar, Lacey T. Ngo, Mariana Gomez De la Espriella, Tasaduq N. Fazili, Jasmine Y. Jackson-Akers, Georgi Guruli, Giuseppe Orlando
Summary: We developed a method to detect COVID-19 disease using urine specimens by analyzing the urine 'molecular fingerprint' representing systemic reactions to infection. The method showed high accuracy in detecting COVID-19 and assessing disease severity based on urine Raman spectra.
Article
Biochemistry & Molecular Biology
Laricca Y. London, Joseph Aubee, Jalisa Nurse, Karl M. Thompson
Summary: The study identified RyhB and FnrS as small RNA regulators that activate the expression of RseA from the RseA(P3) transcript, overcoming redundancy in sigma(E)-dependent stress response in E. coli and related bacteria.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Infectious Diseases
Victor Gravrand, Frederic Ariey, Laura Terracol, Helene Poupet, Alexandra Doloy, Claire Poyart, Hedi Mammeri
Summary: This study identified a mutated Serratia marcescens strain that is resistant to multiple antibiotics. The resistance is caused by a mutation in the cpxA gene, which results in constitutive activation of the Cpx pathway. This finding sheds new light on the role of altered two-component systems in bacterial survival.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Matthias Winkle, Victor M. Hernandez-Rocamora, Karthik Pullela, Emily C. A. Goodall, Alessandra M. Martorana, Joe Gray, Ian R. Henderson, Alessandra Polissi, Waldemar Vollmer
Summary: Research on E. coli has revealed that the attachment of Lpp to peptidoglycan in Gram-negative bacteria is a dynamic process, where detachment of Lpp can be beneficial under certain stress conditions. Furthermore, the enzyme DpaA, identified in this study, is present in many Gram-negative bacteria and may have different substrates in these species.
Article
Gastroenterology & Hepatology
Danielle D. Munhoz, Amanda C. Richards, Fernanda F. Santos, Matthew A. Mulvey, Roxane M. F. Piazza
Summary: Pathogenic subsets of Escherichia coli include diarrheagenic (DEC) strains and extraintestinal pathogenic E. coli (ExPEC) strains. The newly reclassified hybrid strain BA1250 possesses genetic features of both DEC and ExPEC and has the potential to cause disease outside of the gut. The adhesin E. coli common pilus (ECP) acts as a colonization and virulence factor for BA1250, and changes in its activation may affect the fitness of the strain.
Article
Biochemistry & Molecular Biology
Henry Arceo-Ruiz, Elba Xochitiotzi-Flores, Hector Garcia-Ortega, Norberto Farfan, Rosa Santillan, Susana Rincon, Alejandro Zepeda
Summary: A Co metal-organic framework called Co-MTPhPyP assembled from TPhPyP was successfully synthesized and characterized by various techniques. The Co-MTPhPyP showed promising sorption properties for the removal of Pb(II) and Cu(II) in aqueous solution. The kinetic studies indicated a maximum adsorption capacity of 458.8 mg g(-1) for Pb(II) after 30 minutes of exposure.
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Oncology
Mahlatse Mokgaetjie Ledwaba, Ngwanabjala Bridged Magaela, Knowledge Siyabonga Ndlovu, John Mack, Tebello Nyokong, Muthumuni Managa
Summary: Pathogenic microorganisms can cause infection by transferring antibiotic resistance genes. This study investigated the antimicrobial activity of different types of porphyrins on E. coli and found that conjugating porphyrins to graphene quantum dots enhances their effectiveness. The electronic structure and optical properties of the porphyrins were analyzed using density functional theory calculations.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Chemistry, Analytical
Jing Cui, Yu Zhang, Kan Lun, Baiwei Wu, Linghao He, Minghua Wang, Shaoming Fang, Zhihong Zhang, Liming Zhou
Summary: In this study, a two-dimensional porphyrin-based covalent organic framework was used to construct an aptamer-based electrochemical sensor for the detection of E.coli. The sensor demonstrated a low detection limit, excellent stability, and reproducibility, making it a promising technology for food safety applications.
Article
Biochemistry & Molecular Biology
Carlos Diaz-Uribe, Daily Rangel, William Vallejo, Roger Valle, Yoan Hidago-Rosa, Ximena Zarate, Eduardo Schott
Summary: Photodynamic therapy (PDT) shows promise in treating various diseases. The singlet oxygen quantum yield (φ) is an important factor in determining the effectiveness of potential photosensitizers (PS) for PDT. In this study, the φ values of three photosensitizers, including metal-free tetrahydroxyphenyl porphyrin (THPP), THPP-Zn, and THPP-V metal complexes, were measured, and their biological activity against Escherichia coli was evaluated. The results showed that the metal-porphyrin complexes exhibited the highest activity against Escherichia coli at the highest concentration, and their activity was better than the free base ligand. The study also revealed a positive correlation between the φ values and the inhibitory activity against Escherichia coli, with THPP-Zn > THPP > THPP-V in terms of both φ values and inhibitory activity. Furthermore, quantum chemical calculations provided insights into the electronic and optical properties of THPP-Zn, confirming the most likely energy transfer pathway involved in singlet oxygen generation.
Article
Microbiology
S. Legood, D. Seng, I. G. Boneca, N. Buddelmeijer
Summary: This study demonstrates the essential role of Lgt, an enzyme in proteobacteria, in cell growth and viability. The absence of Lgt leads to severe growth and morphological defects that can be restored by expressing Lgt. It is also shown that Lpp is not the main cause of cell death under conditions of Lgt depletion, highlighting the importance of other lipoproteins in cell envelope biogenesis and cell viability.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valentina Cappelletti, Thomas Hauser, Ilaria Piazza, Monika Pepelnjak, Liliana Malinovska, Tobias Fuhrer, Yaozong Li, Christian Doerig, Paul Boersema, Ludovic Gillet, Jan Grossbach, Aurelien Dugourd, Julio Saez-Rodriguez, Andreas Beyer, Nicola Zamboni, Amedeo Caflisch, Natalie de Souza, Paola Picotti
Summary: The global protein structural readout based on limited proteolysis-mass spectrometry (LiP-MS) can detect functional alterations in biological processes with high resolution and coverage simultaneously and in situ. Analysis of structural barcodes can capture changes in enzyme activity, phosphorylation, protein aggregation, complex formation, and more.
Article
Multidisciplinary Sciences
Bram Van den Bergh, Hannah Schramke, Joran Elie Michiels, Tom E. P. Kimkes, Jakub Leszek Radzikowski, Johannes Schimpf, Silke R. Vedelaar, Sabrina Burschel, Liselot Dewachter, Nikola Loncar, Alexander Schmidt, Tim Meijer, Maarten Fauvart, Thorsten Friedrich, Jan Michiels, Matthias Heinemann
Summary: Antibiotic persisters are phenotypic variants within an isogenic bacterial population that are transiently tolerant to antibiotic treatment. This study provides evidence that cytoplasmic acidification, amplified by a compromised respiratory complex I, can act as a signaling hub for perturbed metabolic homeostasis in antibiotic persisters.
NATURE COMMUNICATIONS
(2022)