Article
Engineering, Biomedical
Qi Tang, Peng Tan, Zhaolai Dai, Tao Wang, Shenrui Xu, Yakun Ding, Junqi Jin, Xin Zhang, Yucheng Zhang, Chenlong Zhou, Zitian Yue, Huiyang Fu, Junshu Yan, Xi Ma
Summary: This study investigates the association between hydrophobicity and cell-penetrating peptide delivery efficiency, and successfully develops chimeric peptides P3I7 and P3L7 with strong antibacterial activity and low cytotoxicity. These peptides can effectively penetrate cells to eliminate intracellular pathogens mainly through endocytosis. The mechanism of membrane destruction makes them fast sterilizers and less prone to drug resistance. In vivo trials confirm their good biocompatibility and antibacterial infection effects, indicating their potential as affordable and effective antimicrobial agents against intracellular bacterial infections.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Analytical
Hyunhee Lee, Sungtae Yang
Summary: Research on dimerization to enhance antimicrobial activity of peptides found that the dimer of buforin II showed increased antibacterial potency without significant cytotoxicity, especially effective against multiple antibiotic-resistant bacterial isolates.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2021)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Biochemistry & Molecular Biology
Philip M. Ferguson, Maria Clarke, Giorgia Manzo, Charlotte K. Hind, Melanie Clifford, J. Mark Sutton, Christian D. Lorenz, David A. Phoenix, A. James Mason
Summary: The pharmacodynamic profile and in vivo synergy of antimicrobial peptides (AMPs) play important roles in limiting resistance evolution and ensuring their conservation. Research has shown that the antibacterial potency of temporin L can be synergistically increased when combined with temporins B and A. This synergistic effect may be due to altered interaction with the bacterial plasma membrane. Furthermore, the addition of buforin II can further enhance the cooperativity between temporins B and L. Understanding the mechanism of synergy between AMPs from the same organism can lead to the development of antibiotic combinations with advantageous pharmacodynamic properties.
Article
Pharmacology & Pharmacy
Pankhuri Narula, Sankar Kiruthika, Shruti Chowdhari, Perumal Vivekanandan, Archana Chugh
Summary: We investigate the role of Tachyplesin (Tpl), a marine antimicrobial cell-penetrating peptide, as an anti-HBV agent. Our findings demonstrate the internalization of FITC-Tpl in both Huh7 and HepG2 cell lines. Tpl inhibits the expression of HBV proteins and reduces levels of HBV pre-core RNA and HBV pregenomic RNA, suggesting its potential as an effective therapeutic peptide against HBV.
Article
Chemistry, Medicinal
Jung-Kil Seo, Dong-Gyun Kim, Ji-Eun Lee, Kwon-Sam Park, In-Ah Lee, Ki-Young Lee, Young-Ok Kim, Bo-Hye Nam
Summary: Five arginine-rich analogs were designed based on American oyster defensin (AOD), with A3 and A4 showing potent antimicrobial activity against various organisms without cell toxicity. These analogs possibly interact with DNA or DNA amplification reactions instead of directly acting on bacterial membranes, suggesting potential for novel antimicrobial agents.
Article
Biochemistry & Molecular Biology
Sergey V. Kravchenko, Pavel A. Domnin, Sergei Y. Grishin, Nikita A. Vershinin, Elena V. Gurina, Anastasiia A. Zakharova, Viacheslav N. Azev, Leila G. Mustaeva, Elena Y. Gorbunova, Margarita I. Kobyakova, Alexey K. Surin, Roman S. Fadeev, Olga S. Ostroumova, Svetlana A. Ermolaeva, Oxana V. Galzitskaya
Summary: Combining antimicrobial peptides with cell-penetrating peptides has potential in enhancing antimicrobial activity against various bacterial strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Anselm F. L. Schneider, Marina Kithil, M. Cristina Cardoso, Martin Lehmann, Christian P. R. Hackenberger
Summary: The challenge of delivering proteins into cells has been addressed by conjugating arginine-rich cell-penetrating peptides to the cell surface, enabling efficient delivery of protein-CPP conjugates. The use of thiol-reactive cell-penetrating peptides allows for simple co-incubation to deliver thiol-containing proteins, greatly simplifying protein and antibody delivery with minimal genetic or chemical engineering.
Article
Pharmacology & Pharmacy
Jana Vanova, Barbora Ciharova, Alzbeta Hejtmankova, Chandra Prakash Epperla, Petr Skvara, Jitka Forstova, Marie Hubalek Kalbacova, Hana Spanielova
Summary: The utilization of different cell-penetrating peptides (CPPs) has been investigated to improve the intracellular delivery of nanoparticles. The results suggest that KH27K has the potential to enhance cytosolic release, while LAH4 requires noncovalent modification for high transduction and cytosolic delivery. These findings are valuable for the development of nanoparticle-based delivery systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Roser Segovia, Judith Sole, Ana Maria Marques, Yolanda Cajal, Francesc Rabanal
Summary: Antibiotic resistance poses a major public health challenge, particularly with Gram-negative multidrug-resistant bacteria. Researchers have developed synthetic analogues inspired by natural polymyxins to improve activity and reduce toxicity, focusing on modifications in the hydrophobic domains. The analogues showed selective effects on anionic membranes and a mechanism of action based on the alteration of membrane function, suggesting a promising approach for the design of safer and more selective antibiotics.
Article
Medicine, Research & Experimental
Haruya Yagi, Takumi Tomono, Yuma Handa, Natsuki Saito, Masami Ukawa, Kohei Miyata, Koichi Shigeno, Shinji Sakuma
Summary: This study investigates the use of cell-penetrating peptides anchored to polymeric platforms as an absorption enhancer for delivering biologics via mucosal routes. Results show that hyaluronic acid modified with L-octaarginine significantly enhances the mucosal absorption of protein drugs in the nasal cavities. The lung mucosa and oral mucosa were found to be suitable routes for mucosal absorption of biologics.
MOLECULAR PHARMACEUTICS
(2023)
Article
Food Science & Technology
Shuai Wang, Yujiao Zhang, Mandlaa, Ziyu Sun, Zhongjun Chen
Summary: This study focused on an antimicrobial peptide (APT) produced by Lactobacillus ALAC-4, isolated from traditional fermented sour porridge in Inner Mongolia. The APT showed significant anti-growth activity against most foodborne microorganisms, especially Candida albicans. The study found that APT damaged the cell walls and decreased the metabolic activity and enzyme activity within Candida albicans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Cell Biology
Xiakun Wang, Hongcheng Duan, Min Li, Wei Xu, Lin Wei
Summary: Wound-healing-promoting peptides derived from amphibians, particularly salamanders and frogs, have been characterized and shown to effectively accelerate skin wound healing and photodamage repair. They promote the proliferation and migration of keratinocytes and fibroblasts, recruit immune cells to the wound site, and regulate immune responses, thereby playing a crucial role in wound healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Corina Ciobanasu
Summary: Membrane active peptides have potential in targeted drug delivery systems, and understanding their uptake mechanism is crucial for peptide therapeutics development and clinical use. By studying the dynamics and kinetics of peptides on model membranes, relevant information on peptide uptake steps can be obtained.
Article
Biochemistry & Molecular Biology
Chan Zhang, Rui-Kai Zhang, Ying Feng, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: In this study, a fish-derived antimicrobial peptide (AMP), CATH_BRALE, was expressed in Chlamydomonas reinhardtii as a recombinant peptide with stable expression and broad-spectrum antibacterial activity. It showed strong thermostability and pH stability, and did not exhibit toxicity on different cell lines. The results demonstrate the potential of using C. reinhardtii as a host for producing biologically active CATH_BRALE.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Giovanni Pacini, Bo Ahren
Summary: Tirzepatide stimulates both insulin secretion and glucose effectiveness, with stimulation of glucose effectiveness being the prominent process to reduce glucose.
Article
Biochemistry & Molecular Biology
Si -yu Wang, Yu-zhe Zhang, Xiao-han Liu, Xue-ci Guo, Xiao-fang Wang, Feng-tong Han, Yao Zhang, Chang -lin Wang
Summary: In the formalin pain test, the EM-2 analogs EM-2-Me, EM-2-Et, and EM-2-Bu showed significant analgesic effects with reduced tolerance and gastrointestinal side effects. These effects were mediated through central opioid mechanisms, with EM-2-Me possibly involving dynorphin A release and EM-2-Bu directly activating multiple opioid receptors.