Article
Chemistry, Physical
Fengyi Cao, Gangqing Ma, Lin Mei, Genxing Zhu, Meng Song, Qi Qin, Mingli Jiao
Summary: In this study, a cysteine-containing PAF26 peptide derivative was designed to develop a covalently crosslinked antimicrobial hydrogel, which showed strong crosslinked networks and excellent antimicrobial abilities against Candida albicans, Staphylococcus aureus, and Escherichia coli.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Microbiology
Alexis Proutiere, Laurence du Merle, Marta Garcia-Lopez, Corentin Leger, Alexis Voegele, Alexandre Chenal, Antony Harrington, Yftah Tal-Gan, Thomas Cokelaer, Patrick Trieu-Cuot, Shaynoor Dramsi
Summary: This study reveals that Streptococcus gallolyticus subsp. gallolyticus (SGG) enhances its colonization of the murine colon under tumoral conditions by producing gallocin A, a two-peptide bacteriocin. The study also characterizes the mechanisms of action and resistance of gallocin A, and emphasizes the importance of the interaction between GllA1 and GllA2 peptides.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Lanhua Yi, Ping Zeng, Jun Liu, Kwok-Yin Wong, Edward Wai-Chi Chan, Yanbing Lin, Kin-Fai Chan, Sheng Chen
Summary: The antimicrobial peptide zp37 showed strong bactericidal effect against E. coli O157:H7, causing cell deformation by affecting the cell membrane and binding to DNA in the cytoplasm. Experiments demonstrated low toxicity of zp37 both in vitro (HEK293 cell) and in vivo (Galleria mellonella) assays.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Hongyan Zhang, Sha Liu, Xindan Li, Wenjun Wang, Lili Deng, Kaifang Zeng
Summary: Research has shown that thanatin, P W1, and M-S can disrupt the hyphae of Geotrichum citri-aurantii, as well as inhibit its growth and synthesis by interacting with its DNA.
Article
Infectious Diseases
Amelia Richter, Darcy Sutherland, Hossein Ebrahimikondori, Alana Babcock, Nathan Louie, Chenkai Li, Lauren Coombe, Diana Lin, Rene L. Warren, Anat Yanai, Monica Kotkoff, Caren C. Helbing, Fraser Hof, Linda M. N. Hoang, Inanc Birol
Summary: Antimicrobial peptides (AMPs) are promising biological molecules for developing new therapeutics against antimicrobial resistance. In this study, 51 AMP candidates were bioinformatically identified and characterized. These AMPs demonstrated activity against bacterial pathogens and low toxicity to human cells. Sequence and structural analysis also predicted their antimicrobial activity.
Article
Biochemistry & Molecular Biology
Emily R. Forster, Xinglin Yang, Albert K. Tai, Howard C. Hang, Aimee Shen
Summary: In this study, bile acid-interacting proteins in C. difficile were identified using chemical proteomics, and a previously uncharacterized MerR family protein CD3583 (now known as BapR) was discovered as a putative bile acid-sensing transcription regulator. BapR specifically binds to and is stabilized by lithocholic acid (LCA) in C. difficile, and regulates the expression of several gene clusters, potentially involved in metabolic adaptation.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Huanyu Tao, Qilong Wu, Xuejun Zhao, Peicong Lin, Sheng-You Huang
Summary: MODPEP2.0 is an efficient tool for sampling cyclic peptide structures, which can quickly generate conformations of cyclic peptides with considerable accuracy and computational efficiency compared to other sampling algorithms.
JOURNAL OF CHEMINFORMATICS
(2022)
Article
Chemistry, Medicinal
Ruka Yamauchi, Kenichi Kawano, Yousuke Yamaoka, Aoi Taniguchi, Yoshiaki Yano, Kiyosei Takasu, Katsumi Matsuzaki
Summary: Antibiotics are not effective against certain Gram-negative bacteria due to their low permeability to the outer membrane. The coadministration of antibiotics and antimicrobial peptides is a strategy to enhance their antibacterial activity, but their different pharmacokinetics may limit their efficacy in clinical settings. This study introduced antimicrobial peptide-antibiotic conjugates as a novel approach to improve the antimicrobial activity of antibiotics with low outer membrane permeability.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Carlos F. R. C. Guimaraes, Amanda S. Felix, Tiago A. S. Brandao, Marcelo P. P. Bemquerer, Dorila Pilo-Veloso, Rodrigo M. M. Verly, Jarbas M. M. Resende
Summary: Peptides are interesting alternatives for various applications, especially antimicrobial sequences due to antibiotic resistance. Although dimeric peptides show promise with strong biological activities and degradation resistance, there are relatively few studies due to synthesis obstacles. In this study, we investigate the dimerization of a cysteine-containing sequence to obtain a homodimeric antimicrobial peptide, and propose different dimerization protocols in selected environments. The results show that the reaction medium plays a major role in the formation of the disulfide bond.
Article
Biochemistry & Molecular Biology
Miho Emori, Nobutaka Numoto, Akane Senga, Gert-Jan Bekker, Narutoshi Kamiya, Yuma Kobayashi, Nobutoshi Ito, Fusako Kawai, Masayuki Oda
Summary: The study demonstrated that Cut190*SS increased thermal stability while maintaining enzymatic function, attributed to the formation of a disulfide bond by Cys250 and Cys296. Results indicated that although the metal-binding properties of Cut190*SS series differed, the enzyme retained its ability to utilize Ca2+ to accelerate conformational change, thereby preserving its functionality.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Chemistry, Multidisciplinary
Hamid Goudarziafshar, Somaieh Yousefi, Yunes Abbasi Tyula, Michal Dusek, Vaclav Eigner
Summary: A new binuclear copper(I) Schiff base complex was synthesized using a template method, and its unique structural features and intermolecular interactions were revealed. The complex was found to bind with CT-DNA and exhibit significant antibacterial activities.
Review
Pharmacology & Pharmacy
Taylor B. Smallwood, Richard J. Clark
Summary: Animal venoms are complex mixtures of bioactive molecules, with disulfide-rich peptides being a major component. The study of venom-derived drugs has led to advancements in treating diseases such as type 2 diabetes and chronic pain. New techniques are expanding the pipeline of venom-derived peptides, offering potential for therapeutic applications and increased understanding of molecular targets.
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Seong-Cheol Park, Jong-Kook Lee, Young-Min Kim, Jung Ro Lee
Summary: Due to the emergence of drug-resistant pathogens, scientists have discovered antimicrobial peptides (AMPs) with high antibacterial activity and cell selectivity. By modifying the amino acids in the peptide chain, they have enhanced the antimicrobial effects and salt tolerance, while reducing cytotoxicity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Food Science & Technology
Zijin Yuan, Jude Juventus Aweya, Jian Li, Zhongyan Wang, Shiying Huang, Mingjing Zheng, Linfan Shi, Shanggui Deng, Shen Yang
Summary: This study identified a novel antimicrobial peptide, LCMHC, from the myosin heavy chain of Larimichthys crocea and found that it exhibited strong antibacterial activity against Staphylococcus aureus when combined with low-intensity ultrasound treatment. The peptide was able to improve the cell membrane permeability of S. aureus and assist in the binding of DNA when combined with ultrasound.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shiyi Wang, Yiwen Zhao, Shufen Mao, Jiang Zhu, Yangyang Zhan, Dongbo Cai, Xin Ma, Dong Wang, Shouwen Chen
Summary: Disulfide bonds in proteins significantly affect folding efficiency by limiting conformational space. Inefficient formation of disulfide bonds is the main factor limiting enzyme activity and stability. This study aimed to increase the activity of disulfide-bond-containing proteins by promoting disulfide bond formation in Bacillus licheniformis. By introducing glutamate decarboxylase GAD from Escherichia coli into B. licheniformis, and successively overexpressing disulfide isomerase and oxidoreductase from different sources, the catalytic efficiency of GAD was improved. The engineered B. licheniformis showed significantly increased GAD activity (from 10.4 U/mg to 80.0 U/mg) and demonstrated adaptability for other disulfide-bond-containing proteins, such as UDP-glucosyltransferase from Arabidopsis thaliana. This work demonstrates the importance of disulfide bond formation for protein activity in B. licheniformis and provides a promising platform for expressing disulfide-bond-containing proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Takahiro Muraoka, Tatsuya Shima, Takashi Kajitani, Norihisa Hoshino, Estelle Morvan, Axelle Grelard, Erick J. Dufourc, Takanori Fukushima, Tomoyuki Akutagawa, Kota Nabeya, Kazushi Kinbara
CHEMISTRY-AN ASIAN JOURNAL
(2019)
Article
Biochemical Research Methods
Victor N. D. Carvalho, Andreea Hertanu, Axelle Grelard, Samira Mchinda, Lucas Soustelle, Antoine Loquet, Erick J. Dufourc, Gopal Varma, David C. Alsop, Pierre Thureau, Olivier M. Girard, Guillaume Duhamel
JOURNAL OF MAGNETIC RESONANCE
(2020)
Article
Chemistry, Analytical
Regine Dazzoni, Corinne Bure, Estelle Morvan, Axelle Grelard, Celine Gounou, Jean-Marie Schmitter, Antoine Loquet, Banafshe Larijani, Erick J. Dufourc
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Regine Dazzoni, Axelle Grelard, Estelle Morvan, Anthony Bouter, Christopher J. Applebee, Antoine Loquet, Banafshe Larijani, Erick J. Dufourc
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Erick J. Dufourc
Summary: Membrane nanoobjects such as Bicelles and Nanodiscs are important tools for studying biomembrane properties, providing various membranous platforms for structural biology research. Internal dynamics of these nanoobjects is similar to liposomes, offering potential for studying the 3D structure, topology, and dynamics of membrane proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Biochemistry & Molecular Biology
Caroline Le Senechal, Mathilde Puges, Christophe Barthe, Patricia Costaglioli, Caroline Tokarski, Corinne Bure, Sebastien Vilain
Summary: Bacteria form multicellular and resistant structures known as biofilms. Phospholipids, particularly PE 18:1-18:1, play a critical role in the attachment phase of biofilm formation. Different strains of Pseudomonas aeruginosa have distinct phospholipid profiles, with potential implications for developing strategies to combat biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Michele Dai, Evangelos Georgilis, Guillaume Goudounet, Bertrand Garbay, Jan Pille, Jan C. M. van Hest, Xavier Schultze, Elisabeth Garanger, Sebastien Lecommandoux
Summary: This study reports the design and study of an ELP diblock library based on ELP-[M1V3-i]-[I-j], which showed a thermal phase transition with three distinct regimes by selectively oxidizing methionines of ELP-[M1V3-i] within the different diblocks structures.
Review
Biochemistry & Molecular Biology
Erick J. Dufourc
Summary: Polyphenols have been a part of human culture for about 6000 years. How tannins interact with salivary proteins, lipids, and oral membranes affects taste and astringency. Ethanol content in wines may enhance tannins' actions, while cholesterol in oral membranes may prevent disordering.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Agricultural Engineering
Estelle Morvan, Brice Kauffmann, Axelle Grelard, Antoine Loquet, Anne-Julie Dixon, Erick J. Dufourc, Denis Rontein
Summary: This study reports a new eco-responsible method to produce (E)-(R)-4-thujanol crystals on a kilogram scale, which may open up new opportunities for the flavor, fragrance and pharmaceutical industries.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Sophie Maria, Laurent Bonneau, Benjamin Fould, Gilles Ferry, Jean Albert Boutin, Charlotte Cabanne, Xavier Santarelli, Gilles Joucla
Summary: In this study, three culture methods were compared to determine the perfusion process parameters for a CHO cell line producing a monoclonal antibody. The push-to-low (PTL) and perfusion-without-bleeding (PWB) methods resulted in lower CSPRmin estimations, suggesting a three-step approach to determine the perfusion parameters.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biology
Daniel Jerico, Karol M. Cordoba, Ana Sampedro, Lei Jiang, Gilles Joucla, Charlotte Cabanne, Jose Luis Lanciego, Paolo G. Martini, Pedro Berraondo, Matias A. Avila, Antonio Fontanellas
Summary: Rare diseases, especially monogenic diseases, have attracted pharmaceutical companies' interest in drug research. Acute intermittent porphyria is one of these rare diseases, and there are already some treatment methods, but there are still many unanswered questions.
Article
Chemistry, Analytical
Estelle Morvan, Nada Taib-Maamar, Axelle Grelard, Antoine Loquet, Erick J. Dufourc
Summary: Understanding the membrane dynamics of complex systems is crucial to understanding their function. This study explores NMR methods to filter highly mobile molecular parts from more restricted environments. The researchers developed a quantitative MAS C-13 NMR approach coupled with CP and/or INEPT techniques to study rigid and fluid model membranes. They found that INEPT can detect only very mobile lipid headgroups in gel phases, while CP is required to detect the rigid parts. The study also reveals the potential of C-13 NMR chemical shift to monitor order-disorder phase transitions and provide information about membrane dynamics without isotopic labeling.
ANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Lourdes Monica Bravo-Anaya, Julien Rosselgong, Karla Gricelda Fernandez-Solis, Ye Xiao, Amelie Vax, Emmanuel Ibarboure, Anna Ruban, Coralie Lebleu, Gilles Joucla, Bertrand Garbay, Elisabeth Garanger, Sebastien Lecommandoux
Summary: Hybrids of synthetic polymers and biopolymers, such as cationic ELPs, were successfully obtained by chemoselectively modifying methionine residues to introduce alkyne groups and coupling cationic oligomers through Huisgen azide-alkyne cycloaddition reaction. The characterization of these hybrid cationic ELPs by H-1 NMR, zeta-potential and SEC analyses showed their purity, degree of functionalization, overall charge, and molar mass. Preliminary biological tests demonstrated the efficient internalization of genetic material into living cells using these hybrid cationic ELPs.
Meeting Abstract
Biophysics
A. Saad, D. Martinez, L. Frey, E. J. Dufourc, B. Habenstein, S. Bibow, R. Riek, A. Loquet
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Meeting Abstract
Biophysics
Ahmad Saad, Denis Martinez, Lukas Frey, Erick J. Dufourc, Birgit Habenstein, Stefan Bibow, Roland Ride, Antoine Loquet
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Giovanni Pacini, Bo Ahren
Summary: Tirzepatide stimulates both insulin secretion and glucose effectiveness, with stimulation of glucose effectiveness being the prominent process to reduce glucose.
Article
Biochemistry & Molecular Biology
Si -yu Wang, Yu-zhe Zhang, Xiao-han Liu, Xue-ci Guo, Xiao-fang Wang, Feng-tong Han, Yao Zhang, Chang -lin Wang
Summary: In the formalin pain test, the EM-2 analogs EM-2-Me, EM-2-Et, and EM-2-Bu showed significant analgesic effects with reduced tolerance and gastrointestinal side effects. These effects were mediated through central opioid mechanisms, with EM-2-Me possibly involving dynorphin A release and EM-2-Bu directly activating multiple opioid receptors.
