Article
Parasitology
Yihan Wu, Zhu Ying, Jing Liu, Zhepeng Sun, Shuang Li, Qun Liu
Summary: This study identified and characterized the ANT protein in T. gondii. Depletion of TgANT inhibited parasite growth, impaired mitochondrial and apicoplast biogenesis, and resulted in abnormal cell division.
PARASITES & VECTORS
(2022)
Review
Cell Biology
Elena Frigo, Ludovica Tommasin, Giovanna Lippe, Michela Carraro, Paolo Bernardi
Summary: This article introduces the formation of Ca2+-activated, high-conductance channels by F1FO (F)-ATP synthase and adenine nucleotide translocase (ANT) in the inner membrane of mitochondria from various eukaryotes. The permeability transition (PT), a Ca2+-dependent increase in permeability in the inner mitochondrial membrane, and its molecular mechanisms have been challenging for scientists for the past 70 years. The features of the PT (or lack thereof) in mammals, yeast, fruit flies, brine shrimp, and nematodes, as well as the intrinsic pathway of apoptosis and other forms of cell death, are discussed in this review.
Article
Biochemistry & Molecular Biology
Justin Sui, Jennifer C. Boatz, Jian Shi, Qianjiang Hu, Xiaoyun Li, Yingze Zhang, Melanie Koenigshoff, Corrine R. Kliment
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease characterized by lung scarring and remodeling. This study investigates the role of adenine nucleotide translocases (ANTs) in IPF pathogenesis and finds that the loss of ANT1 leads to mitochondrial dysfunction, increased cellular senescence, and enhanced lung fibrosis. The findings suggest that modulation of ANTs could be a potential therapeutic avenue for limiting pulmonary fibrosis.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Camila Cimadamore-Werthein, Stephany Jaiquel Baron, Martin S. King, Roger Springett, Edmund R. S. Kunji
Summary: The mitochondrial ADP/ATP carrier plays a crucial role in oxidative phosphorylation by importing ADP into the mitochondrial matrix and exporting ATP. Recent studies have revealed that the carrier functions as a monomer with a single substrate binding site and operates with a ping-pong kinetic mechanism. These findings reconcile the discrepancy between the previous assumptions and provide insights into the alternating access mechanism of the carrier.
Article
Cell & Tissue Engineering
X. Du, Z. Jiang, G. Fang, R. Liu, X. Wen, Y. Wu, S. Hu, Z. Zhang
Summary: This study aimed to investigate the role and mechanism of meniscal cell lysate (MCL) in fibroblast-like synoviocytes (FLSs) and osteoarthritis (OA). MCL was found to be substantially enriched in the synovial fluid of OA patients, promoting the release of inflammatory cytokines from FLSs through MCL phagocytosis. ANT3 was up-regulated in MCL and OA-FLSs, corresponding to impaired mitochondrial function and cell viability. Silencing ANT3 restored mitochondrial homeostasis, alleviating synovial inflammation and preventing early-stage OA.
BONE & JOINT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Pavla Pruchova, Klara Gotvaldova, Katarina Smolkova, Lukas Alan, Blanka Holendova, Jan Tauber, Alexander Galkin, Petr Jezek, Martin Jaburek
Summary: This study reveals the antioxidant and anti-inflammatory roles of iPLA(2)gamma in the brain. It demonstrates that iPLA(2)gamma is activated by tert-butylhydroperoxide and releases free fatty acids, which leads to an increase in respiratory rate and a decrease in mitochondrial superoxide production. It also shows that iPLA(2)gamma plays a key role in the remodeling of cardiolipins in the brain.
Article
Physiology
Tomas A. Schiffer, Liza Loef, Radiosa Gallini, Masood Kamali-Moghaddam, Mattias Carlstroem, Fredrik Palm
Summary: The study reveals a dynamic interaction between ANT2 and UCP2 that is regulated by mitochondrial respiratory activity, potentially impacting mitochondrial efficiency and cellular substrate utilization.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Stephany Jaiquel Baron, Martin S. King, Edmund R. S. Kunji, Tom J. J. Schirris
Summary: An increasing number of commonly prescribed drugs are known to interfere with mitochondrial function, causing cellular toxicity. However, the underlying mechanisms are largely unknown, and it has been found that only a subset of the 18 published AAC inhibitors can inhibit cellular respiratory capacity, raising questions about the effectiveness of all compounds in inhibiting this central metabolic process. Further research has provided new insights into the chemical compound features important for inhibition of mitochondrial transport proteins.
Article
Chemistry, Medicinal
Ekaterina S. Kharechkina, Anna B. Nikiforova, Konstantin N. Belosludtsev, Tatyana Rokitskaya, Yuri N. Antonenko, Alexey G. Kruglov
Summary: Pioglitazone is an insulin-sensitizing antidiabetic drug that can affect glucose and lipid metabolism through mitochondrial targets. Research has shown that pioglitazone can decrease mitochondrial membrane potential, impact oxidative phosphorylation coupling, activate proton transport, among other effects, which play a crucial role in both therapeutic and adverse effects of the drug.
Article
Cell Biology
Xavier R. Chapa-Dubocq, Jorge F. Garcia-Baez, Jason N. Bazil, Sabzali Javadov
Summary: This study investigates the modulation of mitochondrial metabolism and function by changes in matrix Ca2+. It identifies the main mechanism responsible for mitochondrial Ca2+ overload and proposes a potential role of adenine nucleotide translocator (ANT) in the gating of mitochondrial permeability transition pores (PTP).
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Nutrition & Dietetics
Alexia Gomez Rodriguez, Emanuela Talamonti, Alba Naudi, Anastasia V. Kalinovich, Anna M. Pauter, Gustavo Barja, Tore Bengtsson, Anders Jacobsson, Reinald Pamplona, Irina G. Shabalina
Summary: The production of endogenous DHA is crucial for the normal function of mouse liver mitochondria and may be relevant for human metabolism as well.
Article
Pharmacology & Pharmacy
Pengfei Zhang, Xinyu Cheng, Huimin Sun, Yajing Li, Wuxuan Mei, Changchun Zeng
Summary: ATR treatment can reduce the manifestation of fatty liver in HFD mice, decrease the accumulation of LDs in the liver, promote the activation of AMPK which leads to decreased mTOR activity, and facilitate autophagosome formation for accelerated lipid degradation.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Martin Jaburek, Pavla Pruchova, Blanka Holendova, Alexander Galkin, Petr Jezek
Summary: The iPLA2 gamma protein, also known as PNPLA8, plays crucial roles in the mitochondrial matrix, including antioxidant functions, lipid peroxidation association, membrane remodeling, and generation of specific lipid second messengers. It is directly stimulated by H2O2, activated through redox signaling, and works synergistically with mitochondrial uncoupling proteins to maintain cellular redox balance. Additionally, it is involved in FA beta-oxidation in pancreatic beta-cells and cytoprotective roles in the heart and brain.
Article
Multidisciplinary Sciences
Andrea Carrer, Ludovica Tommasin, Justina Sileikyte, Francesco Ciscato, Riccardo Filadi, Andrea Urbani, Michael Forte, Andrea Rasola, Ildiko Szabo, Michela Carraro, Paolo Bernardi
Summary: The nature of the mitochondrial permeability transition pore (PTP) remains debated, with the possibility that it can be mediated by F-ATP synthase or adenine nucleotide translocator. Through genetic modifications and cell experiments, researchers identified the essential role of certain mitochondrial subunits in turning the F-ATP synthase into the PTP, and showed that the ANT can contribute to channel formation in the absence of an assembled F-ATP synthase.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Jean Finlayson, Neusha Barakati, Paul R. Langlais, Janet Funk, Rocio Zapata Bustos, Dawn K. Coletta, Moulun Luo, Wayne T. Willis, Lawrence J. Mandarino
Summary: This study characterized ANT1 abundance and acetylation in human skeletal muscle using a novel method with small amounts of tissue samples. The findings indicate that acetylation of Lys23 reduces ADP binding to ANT1, providing insights into the potential effects of acetylation on ANT1 function in human muscle.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Yikui Tian, Melissa Marshall, Brent A. French, Joel Linden, Zequan Yang
BASIC RESEARCH IN CARDIOLOGY
(2015)
Article
Cardiac & Cardiovascular Systems
Yikui Tian, Brent A. French, Irving L. Kron, Zequan Yang
BASIC RESEARCH IN CARDIOLOGY
(2015)
Article
Chemistry, Multidisciplinary
Siva Sai Krishna Dasa, Ryo Suzuki, Michael Gutknecht, Lindsey T. Brinton, Yikui Tian, Erik Michaelsson, Lennart Lindfors, Alexander L. Klibanov, Brent A. French, Kimberly A. Kelly
JOURNAL OF CONTROLLED RELEASE
(2015)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nivedita K. Naresh, Xiao Chen, Eric Moran, Yikui Tian, Brent A. French, Frederick H. Epstein
MAGNETIC RESONANCE IN MEDICINE
(2016)
Article
Cell Biology
Yikui Tian, Bryan A. Piras, Irving L. Kron, Brent A. French, Zequan Yang
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2015)
Article
Cardiac & Cardiovascular Systems
Yikui Tian, Dongfeng Pan, Mahendra D. Chordia, Brent A. French, Irving L. Kron, Zequan Yang
BASIC RESEARCH IN CARDIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
B. A. Piras, Y. Tian, Y. Xu, N. A. Thomas, D. M. O'Connor, B. A. French
Article
Surgery
Yingying Ni, Degang Liang, Yikui Tian, Irving L. Kron, Brent A. French, Zequan Yang
JOURNAL OF SURGICAL RESEARCH
(2018)
Article
Multidisciplinary Sciences
Yikui Tian, Joel Linden, Brent A. French, Zequan Yang
Article
Cardiac & Cardiovascular Systems
Zequan Yang, Yikui Tian, Stuart S. Berr, Brent A. French
CARDIOLOGY RESEARCH AND PRACTICE
(2020)
Article
Cardiac & Cardiovascular Systems
Soham A. Shah, Claire E. Reagan, Brent A. French, Frederick H. Epstein
Summary: The study found that adenosine myocardial perfusion reserve is mainly mediated through the A(2A) receptor, while adenosine Delta T1 is mainly mediated through the A(2A) and A(2B) receptors; adenosine myocardial perfusion reserve is independent of endothelial function, while adenosine Delta T1 is partially dependent on endothelial function; Delta T1 mediated through the A(2A) receptor is endothelial dependent, while Delta T1 mediated through the A(2B) receptor is endothelial independent.
CIRCULATION-CARDIOVASCULAR IMAGING
(2021)
Article
Acoustics
Alyssa B. Becker, Lanlin Chen, Bo Ning, Song Hu, John A. Hossack, Alexander L. Klibanov, Brian H. Annex, Brent A. French
Summary: Mouse models are crucial for developing new therapies for peripheral arterial disease (PAD). In this study, different perfusion measurement methods (LDPI, CEUS, fluorescent microspheres) were compared in a mouse model of PAD. LDPI and CEUS showed similar trends of perfusion recovery until 7 days post-surgery. However, by day 14, LDPI indicated full recovery while CEUS showed only around 50% recovery, which did not improve even after 5 months. Fluorescent microspheres confirmed incomplete perfusion recovery, and histopathology and photoacoustic microscopy provided further evidence of sustained vascular abnormalities.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Article
Cell Biology
Gang Yu, Susmita Chakrabarti, Miroslava Tischenko, Ai-Lan Chen, Zhijie Wang, Hyosuk Cho, Brent A. French, Sathyamangla V. Naga Prasad, Qiuyun Chen, Qing K. Wang
Summary: Brugada syndrome (BrS) is a fatal arrhythmia that causes sudden death. Researchers generated a mouse model of BrS and found that gene therapy can reverse the clinical features of the disease.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Proceedings Paper
Acoustics
Dan Lin, Yikui Tian, Alexander L. Klibanov, Brent A. French, John A. Hossack
2014 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS)
(2014)