Article
Biochemistry & Molecular Biology
Sanja Skulj, Zlatko Brkljaca, Jurgen Kreiter, Elena E. Pohl, Mario Vazdar
Summary: Molecular dynamics simulations of uncoupling proteins present a challenge due to the lack of available structural data. In this study, a combination of homology modelling and MD simulations of UCP2 revealed a structure impermeable to water. Additionally, results from conductance measurements in model membranes suggest that the UCP2 structure modeled from ANT protein possesses key functional elements related to proton transport.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Furkan E. Oflaz, Zhanat Koshenov, Martin Hirtl, Olaf A. Bachkoenig, Wolfgang F. Graier, Benjamin Gottschalk
Summary: Mitochondrial uncoupling proteins UCP1 and UCP2 have different molecular mechanisms for executing their mitochondria uncoupling function, with UCP1 dissipating the proton motive force and UCP2 modulating the permeability of the cristae junction. This mini-review discusses the molecular mechanisms of UCP1 in brown adipose tissue and UCP2 in aged and cancer non-excitable cells, and the synergistic effects of both UCPs with the mitochondrial Ca2+ uptake machinery.
Letter
Plant Sciences
Philippe Fuchs, Elisenda Feixes-Prats, Paulo Arruda, Elias Feitosa-Araujo, Alisdair R. Fernie, Christopher Grefen, Sophie Lichtenauer, Nicole Linka, Ivan de Godoy Maia, Andreas J. Meyer, Soeren Schilasky, Lee J. Sweetlove, Stefanie Wege, Andreas P. M. Weber, A. Harvey Millar, Olivier Keech, Igor Florez-Sarasa, Pedro Barreto, Markus Schwarzlaender
Summary: UCP2 is localized to the Golgi, unlike its close homolog UCP1, which is abundant in the mitochondria.
Article
Cell Biology
Fabiola Olivieri, Francesco Prattichizzo, Fabrizia Lattanzio, Anna Rita Bonfigli, Liana Spazzafumo
Summary: One of the challenges in aging research is to understand how genetics and environmental factors interact to determine the chances of reaching extreme human lifespan in good health. Epigenetic mechanisms provide an explanation for this interaction and the long-term effects of lifestyle on aging. The theory of inflammaging suggests that chronic low-grade inflammation plays a crucial role in age-related diseases and mortality. Studies on centenarians have shown that healthy individuals can upregulate anti-inflammatory responses to cope with inflammaging. Genetic factors and the ability to remodel metabolic pathways are also important for healthy aging. The concept of antifragility, which refers to the ability of complex systems to perform better under stress, may provide insights into the heterogeneous nature of the aging process and its relationship with inflammation.
AGEING RESEARCH REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Chen Feng, Etienne Empweb Anger, Xiong Zhang, Shengdi Su, Chenlin Su, Shuxin Zhao, Feng Yu, Ji Li
Summary: This study suggests that UCP2 may protect HK-2 cells from the toxicity induced by AA I, and reduce the toxicity by decreasing oxidative stress and apoptosis. UCP2 could be a potential target for alleviating AA I-induced nephrotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Haowen Jiang, Rachel L. Greathouse, Sarah Jane Tiche, Man Zhao, Bo He, Yang Li, Albert M. Li, Balint Forgo, Michaela Yip, Allison Li, Moriah Shih, Selene Banuelos, Meng-Ning Zhou, Joshua J. Gruber, Erinn B. Rankin, Zhen Hu, Hiroyuki Shimada, Bill Chiu, Jiangbin Ye
Summary: The Warburg effect is the major metabolic hallmark of cancer. In this study, researchers found that treating neuroblastoma cells with the mitochondrial uncoupler NEN can induce neural differentiation by altering metabolic and epigenetic pathways.
Review
Biochemistry & Molecular Biology
Masa Cater, Lidija Krizancic Bombek
Summary: Accumulation of oxidative damage to DNA and biomolecules is crucial in aging and age-related diseases. Mitochondrial DNA is highly vulnerable to oxidative stress, leading to dysfunction and bioenergetic crisis. Mitochondrial uncoupling proteins (UCPs) protect cells from damage by dissipating the proton gradient required for oxidative phosphorylation. Understanding UCPs' roles is critical in developing strategies to combat type 2 diabetes.
Article
Biochemistry & Molecular Biology
Giselli Scaini, Brittany L. Mason, Alexandre P. Diaz, Manish K. Jha, Jair C. Soares, Madhukar H. Trivedi, Joao Quevedo
Summary: Recent studies have shown that mitochondrial dysfunction and dysregulated neuroinflammatory pathways play a role in major depressive disorder (MDD). This study found differences in markers of mitochondrial dynamics, mitophagy, autophagy, and apoptosis in MDD patients compared to healthy controls, with higher levels of specific proteins associated with mitochondrial dysfunction in MDD patients. Furthermore, inflammation and protein levels related to mitochondrial dynamics and mitophagy were found to influence the severity of depressive symptoms in MDD patients.
MOLECULAR PSYCHIATRY
(2022)
Article
Plant Sciences
Mariana de Lara Campos Arcuri, Alessandra Vasconcellos Nunes-Laitz, Romulo Pedro Macedo Lima, Pedro Barreto, Andressa Nagatani Marinho, Paulo Arruda, Ivan G. Maia
Summary: This study investigated the potential roles of two UCP genes in Arabidopsis thaliana in energy metabolism, root growth, fertility, and ROS homeostasis. Mutants exhibited alterations in ATP/ADP ratios, reduced respiratory rate, and compromised fertility. Increased hydrogen peroxide accumulation in reproductive organs and disturbed ROS homeostasis were observed, indicating the importance of AtUCP1 and AtUCP2 in flower oxidative homeostasis and overall plant fertility.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
David G. Nicholls
Summary: This review examines the mechanisms underlying non-shivering thermogenesis in brown adipose tissue mediated by UCP1, emphasizing the experimental evidence and physiological constraints. It also discusses the nature of endogenous proton leak and evaluates the troubled history of putative novel uncoupling proteins UCP2 and UCP3.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Multidisciplinary Sciences
Ryota Inoue, Takahiro Tsuno, Yu Togashi, Tomoko Okuyama, Aoi Sato, Kuniyuki Nishiyama, Mayu Kyohara, Jinghe Li, Setsuko Fukushima, Tatsuya Kin, Daisuke Miyashita, Yusuke Shiba, Yoshitoshi Atobe, Hiroshi Kiyonari, Kana Bando, A. M. James Shapiro, Kengo Funakoshi, Rohit N. Kulkarni, Yasuo Terauchi, Jun Shirakawa
Summary: This study highlights the molecular link between the increase in UCP2 expression in beta-cells and beta-cell failure, and suggests that targeting the UCP2/AldB axis may be a promising approach for the recovery of beta-cell function.
Article
Cell Biology
Sunad Rangarajan, Morgan L. Locy, Diptiman Chanda, Ashish Kurundkar, Deepali Kurundkar, Jennifer L. Larson-Casey, Pilar Londono, Rushita A. Bagchi, Brian Deskin, Hanan Elajaili, Eva S. Nozik, Jessy S. Deshane, Jaroslaw W. Zmijewski, Oliver Eickelberg, Victor J. Thannickal
Summary: This study provides evidence that elevated levels of the mitochondrial protein UCP2 are associated with age-related diseases, such as idiopathic pulmonary fibrosis. UCP2 is shown to contribute to the generation of reactive oxygen species and altered cellular bioenergetics, as well as induce myofibroblast senescence. The findings suggest that targeting UCP2 could be a potential therapeutic strategy for age-related diseases associated with impaired tissue regeneration and organ fibrosis.
Article
Hematology
Toshiaki Iba, Jean M. Connors, Jerrold H. Levy
Summary: Long COVID (postacute sequelae of COVID-19 [PASC]) refers to the sustained symptoms of fatigue, cognitive disturbance, and muscle ache/weakness in patients who have recovered from acute COVID-19 infection, with a prevalence estimated to be 10 to 20% of convalescent patients. Microclots in circulation may be one of the causes. Despite studies on the pathophysiology of PASC, the exact mechanism remains unclear.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Review
Endocrinology & Metabolism
Chen Huang, Jian Li, Chenliang Zhang
Summary: Pyroptosis, a caspase-dependent cell death, plays a complex role in cancer development. It can be both anti-tumor and pro-tumor. Pyroptosis provides potential new targets for cancer treatment and helps optimize the use of existing drugs. Immunotherapy is a hot research direction in the field of cancer treatment.
MOLECULAR METABOLISM
(2022)
Article
Pharmacology & Pharmacy
Tomoyuki Sato, Akinori Takemura, Kousei Ito
Summary: Mitochondrial injury contributes to severe drug-induced liver injury, with certain drugs inducing mitochondrial permeability transition (MPT) through an uncoupling effect. Using isolated mitochondria from rat liver, it was found that several drugs, including troglitazone, induced MPT and exhibited an uncoupling effect. Analysis revealed a strong correlation between uncoupling intensity and the potential for MPT, offering insights into predicting and reducing the risk of MPT in drug-induced liver injury.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Neurosciences
Caroline M. Voss, Jens V. Andersen, Emil Jakobsen, Olga Siamka, Melis Karaca, Pierre Maechler, Helle S. Waagepetersen
Article
Biochemistry & Molecular Biology
Thierry Brun, Cecilia Jimenez-Sanchez, Jesper Grud Skat Madsen, Noushin Hadadi, Dominique Duhamel, Clarissa Bartley, Lucie Oberhauser, Mirko Trajkovski, Susanne Mandrup, Pierre Maechler
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biotechnology & Applied Microbiology
Ruodan Xu, Mingfei Shi, Jing Li, Ping Song, Ning Li
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Review
Chemistry, Multidisciplinary
Ziyun Li, Mingfei Shi, Ning Li, Ruodan Xu
FRONTIERS IN CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Ruodan Xu, Shizhong Li, Mingfei Shi, Ziyun Li, Yin Wang, Jing Li, Qiang Li, Lasse H. Klausen, An Li, Haiyu Zhao, Menglin Chen, Jingqing Hu, Mingdong Dong, Ning Li
Summary: The peptide-based HDL (pHDL) constructed through microfluidics is a potential therapeutic candidate for managing dyslipidemia and hyperglycemia in atherosclerosis and type 2 diabetes. pHDL predominantly triggers activation of adipose tissue browning, providing new insights into its role in metabolic defects treatment.
Article
Pharmacology & Pharmacy
Mingfei Shi, Bo Peng, An Li, Ziyun Li, Ping Song, Jing Li, Ruodan Xu, Ning Li
Summary: The novel coronavirus disease 2019 (COVID-19) has raised international concern due to its exponential spread, and combined treatment of Traditional Chinese Medicine and modern medicine has shown to reduce mortality rate and improve clinical outcomes. Lian-Hua-Qing-Wen Capsule (LHQWC) and Jin-Hua-Qing-Gan Granule (JHQGG) recommended by China for COVID-19 treatment have broad-spectrum antiviral capacities, targeting viral life cycle and regulating host immune responses differently. LHQWC is more effective in regulating host anti-viral responses, while JHQGG is more potent in modulating viral life cycle, which can be beneficial for patients with different clinical conditions.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Lucie Oberhauser, Cecilia Jimenez-Sanchez, Jesper Grud Skat Madsen, Dominique Duhamel, Susanne Mandrup, Thierry Brun, Pierre Maechler
Summary: Chronic exposure to high glucose and fatty acids can lead to adaptations in pancreatic beta cells, which help preserve their secretion response. The expression pattern of lipid pathway genes and the process of fat storage and mobilization play a crucial role in glucose-stimulated insulin secretion.
Review
Biochemistry & Molecular Biology
Lucie Oberhauser, Pierre Maechler
Summary: This review discusses the role of lipotoxicity and glucotoxicity in the pathophysiology of obesity-related type 2 diabetes, specifically in the loss of beta-cell function. It highlights the effects of free fatty acid metabolism and receptor-mediated signaling related to glucose-stimulated insulin secretion on beta-cell function. Chronic exposure to high glucose and free fatty acids contributes to beta-cell dysfunction, while the glycerolipid/free fatty acid cycle is considered a protective pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mengxia Lin, Yuan Liu, Junwei Gao, Donghui Wang, Dan Xia, Chunyong Liang, Ning Li, Ruodan Xu
Summary: The development of antibacterial wound dressings that co-deliver two antibiotics, ciprofloxacin and tetracycline hydrochloride, in a coaxial nanofiber matrix shows great potential due to their strong antibacterial activity and biocompatibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Ning Li, Yeping Qin, Dan Dai, Pengyu Wang, Mingfei Shi, Junwei Gao, Jinsheng Yang, Wei Xiao, Ping Song, Ruodan Xu
Summary: Psoriasis is a chronic immune-mediated skin disorder, and the optimal treatment is still unsatisfactory. The use of nanomaterials as topical drug carriers may offer potential therapeutic effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dan Xia, Yuan Liu, Wuxiu Cao, Junwei Gao, Donghui Wang, Mengxia Lin, Chunyong Liang, Ning Li, Ruodan Xu
Summary: Coaxial electrospinning nanofibrous patches combining anti-bacterial and anti-inflammation agents have the potential to accelerate wound healing, which is achieved through co-loading and sequential release of drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Integrative & Complementary Medicine
Ruo-Dan Xu, Ming-Fei Shi, Jing Li, Ning Li
Summary: The global outbreak of COVID-19 has had disastrous consequences, with no approved medications currently available. The benefits of traditional Chinese medicine in combating the virus have been observed, and the use of virus-like particle models provides a safe and effective method for Chinese medicine in preventing and controlling newly identified infectious diseases.
WORLD JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Cecilia Jimenez-Sanchez, Thierry Brun, Pierre Maechler
Article
Multidisciplinary Sciences
Min Shang, Federica Cappellesso, Ricardo Amorim, Jens Serneels, Federico Virga, Guy Eelen, Stefania Carobbio, Melvin Y. Rincon, Pierre Maechler, Katrien De Bock, Ping-Chih Ho, Marco Sandri, Bart Ghesquiere, Peter Carmeliet, Mario Di Matteo, Emanuele Berardi, Massimiliano Mazzone
Article
Genetics & Heredity
Karolina Luczkowska, Caroline Stekelenburg, Frederique Sloan-Bena, Emmanuelle Ranza, Giacomo Gastaldi, Valerie Schwitzgebel, Pierre Maechler