Article
Toxicology
Blanca Laffon, Maria Sanchez-Flores, Natalia Fernandez-Bertolez, Eduardo Pasaro, Vanessa Valdiglesias
Summary: This study demonstrates that whole blood samples can be used to assess genotoxicity related to DSB by evaluating the feasibility of the gamma H2AX assay using flow cytometry, and exploring the convenience of PHA stimulation.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ievgeniia Gazo, Roman Franek, Radek Sindelka, Ievgen Lebeda, Sahana Shivaramu, Martin Psenicka, Christoph Steinbach
Summary: Sterlet embryos show varying sensitivity to different genotoxicants, with some inducing DNA damage and activating DDR pathways, while others causing DNA damage without activating known DDR pathways, with effects also being stage-specific in embryo development. This study provides foundational information for future investigation of sterlet DDR pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Linke Tang, Weifeng Yuan, Shitao Li, Xiuyan Ding, Liqian Zhu
Summary: In this study, the interaction between UV-primed global DDR and BoHV-1 productive infection was demonstrated. UV-primed global DDR differentially modulated the transcription of virus genes and stabilization of virus protein. Vice versa, the virus infection may affect UV-primed DDR signaling.
Editorial Material
Cell Biology
Jake Atkinson, Eva Bezak, Ivan Kempson
Summary: DNA double-strand breaks play a fundamental role in cell biology, but there is a lack of a reliable assay for quantitatively visualizing DSBs. Relying on proxy detection methods can lead to misinterpretation of conventional assays, highlighting the need for the development of more accurate DSB detection methods.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mahara Valverde, Aaron Sanchez-Brito
Summary: Early inflammatory environments can impact adipocyte function, leading to sustained activation of DNA damage response, triggering adipocyte dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Nazanin Rahmanian, Mohammad Shokrzadeh, Morteza Eskandani
Summary: The phosphorylation of histone variant H2AX and the formation of gamma H2AX are primary responses to DNA double-strand breaks, serving as robust and sensitive tools for diagnosing DNA damage and repair in pre-clinical drug discovery investigations. Gamma H2AX can also serve as a surrogate marker for drug-induced cytotoxicity, while recent advances in genomic research allow for its detection as a specific biomarker for quantitatively analyzing DNA damages and repair. The use of gamma H2AX as a biomarker shows promise in genotoxicity analyses and the monitoring and management of cancer diseases.
Article
Biochemistry & Molecular Biology
Galina N. Chelomina, Sergey P. Kukla, Viktor P. Chelomin, Pham N. Doanh
Summary: Parasite infection can lead to DNA damage, potentially involving oxidative stress and cancer development; these research findings are important for understanding the pathogenesis mechanisms of parasitic diseases.
Article
Biochemistry & Molecular Biology
Barbara de la Pena Avalos, Nicolas Paquet, Romain Tropee, Yan Coulombe, Hannah Palacios, Justin W. Leung, Jean-Yves Masson, Pascal H. G. Duijf, Eloise Dray
Summary: Efficient DNA repair and limitation of genome rearrangements depend on the crosstalk between different DNA double-strand break (DSB) repair pathways and their synchronization with the cell cycle. This study identifies EYA4 as a protein phosphatase that dephosphorylates RAD51, a key player in homologous recombination (HR) at DSBs. EYA4 regulates RAD51 localization, filament formation, foci formation, and activity through its tyrosine phosphatase activity. The findings have implications for understanding the pathological version of RAD51 in EYA4-overexpressing cancers.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
S. Schumann, H. Scherthan, K. Pfestroff, S. Schoof, A. Pfestroff, P. Hartrampf, N. Hasenauer, A. K. Buck, M. Luster, M. Port, M. Lassmann, U. Eberlein
Summary: This study provides novel data on ex vivo DSB repair in internally irradiated PBMCs of patients before radionuclide therapy, showing efficient repair occurs after internal irradiation with 50 mGy absorbed dose, and that the induction and repair rate after I-131 exposure is comparable to that of external irradiation with gamma- or X-rays.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Xin Ming, Zongyi Bo, Yurun Miao, Huan Chen, Chenyi Bao, Liumei Sun, Rui Xi, Qiuping Zhong, Pu Zhao, Yong-Sam Jung, Yingjuan Qian
Summary: The PRV infection activates ATM and DNA-PK signaling pathways, while the PRV-encoded kinase UL13 interacts with and phosphorylates H2AX. Deletion of UL13 attenuates PRV neuroinvasiveness and virulence in vivo. UL13-mediated H2AX phosphorylation plays a pivotal role in efficient PRV replication and progeny production.
Article
Biochemistry & Molecular Biology
Xiuyan Ding, Weifeng Yuan, Hao Yang, Chang Liu, Shitao Li, Liqian Zhu
Summary: The study demonstrates that BoHV-1 infection disrupts DNA damage repair mediated by 53BP1 and suggests that β-catenin plays a role in restricting both virus replication and DNA damage in A549 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sayanthooran Saravanabavan, Gopala K. Rangan
Summary: Increased DNA damage response signaling in kidney cyst-lining epithelial cells (CECs) may be a target for therapy in ADPKD. However, inhibiting ATM kinase and low-dose cisplatin together does not selectively induce cell death in CECs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Victoria Liedtke, Christian Schroder, Dirk Roggenbuck, Romano Weiss, Ralf Stohwasser, Peter Schierack, Stefan Rodiger, Lysann Schenk
Summary: LEDGF/p75, an important factor in cancer, affects the DNA-damage repair signaling pathway. Depletion of LEDGF leads to reduced cell proliferation and migration, increased sensitivity to chemotherapeutic drugs, and alterations in DDR signaling pathways. Unexpectedly, untreated LEDGF KO cells exhibit increased DNA fragmentation and formation of specific proteins, highlighting the significant role of LEDGF/p75 in homology-directed DNA repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Toxicology
Sherin John Joseph, Kantha Deivi Arunachalam, P. Balakrishna Murthy, Raghavendra Ramalingam, Mohamed Saiyad Musthafa
Summary: This study investigates the effects of uranium on human lymphocytes through acute toxicity research, revealing dose-dependent DNA damage and cell cycle arrest at the G2/M checkpoint, indicating decreased genetic stability. Lower doses of uranium exposure lead to better cell cycle responses and lower genetic damage index in human lymphocytes, suggesting adaptability and repair responses. The findings advance our understanding of uranium's impact on mammalian cells.
TOXICOLOGY IN VITRO
(2021)
Article
Geriatrics & Gerontology
Robertina Giacconi, Blanca Laffon, Solange Costa, Armanda Teixeira-Gomes, Fabrizio Maggi, Lisa Macera, Pietro Giorgio Spezia, Francesco Piacenza, Alexander Buerkle, Maria Moreno-Villanueva, Stefano Bonassi, Vanessa Valdiglesias, Joao Paulo Teixeira, Martijn E. T. Dolle, M. Liset Rietman, Eugene Jansen, Tilman Grune, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Birgit Weinberger, Ewa Sikora, Wolfgang Stuetz, Olivier Toussaint, Florence Debacq-Chainiaux, Antti Hervonen, Mikko Hurme, P. Eline Slagboom, Christiane Schoen, Juergen Bernhardt, Nicolle Breusing, Eduardo Pasaro, Ana Maseda, Laura Lorenzo-Lopez, Jose C. Millan-Calenti, Mauro Provinciali, Marco Malavolta
Summary: This study analyzed the association between TTV viremia and physical frailty and cognitive impairment. The results showed that TTV viremia was associated with physical frailty and cognitive impairment. However, the association varied in different study samples.
Review
Environmental Sciences
Maria Joao Bessa, Fatima Brandao, Fernanda Rosario, Luciana Moreira, Ana Teresa Reis, Vanessa Valdiglesias, Blanca Laffon, Sonia Fraga, Joao Paulo Teixeira
Summary: Several studies have investigated the potential adverse health risks associated with exposure to nanoscale materials. In vitro model systems provide important information about the molecular mechanisms of action, while in vivo studies are crucial for understanding the relationship between dose and adverse effects. This review focuses on the toxicity assessment of inhaled (nano)particulate materials and provides an overview of available human respiratory models and exposure systems for in vitro testing, as well as their advantages, limitations, and current research.
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART B-CRITICAL REVIEWS
(2023)
Article
Biochemical Research Methods
Andrew Collins, Peter Moller, Goran Gajski, Sona Vodenkova, Abdulhadi Abdulwahed, Diana Anderson, Ezgi Eyluel Bankoglu, Stefano Bonassi, Elisa Boutet-Robinet, Gunnar Brunborg, Christy Chao, Marcus S. S. Cooke, Carla Costa, Solange Costa, Alok Dhawan, Joaquin de Lapuente, Cristian Del Bo, Julien Dubus, Maria Dusinska, Susan J. J. Duthie, Naouale El Yamani, Bevin Engelward, Isabel Gaivao, Lisa Giovannelli, Roger Godschalk, Sofia Guilherme, Kristine B. B. Gutzkow, Khaled Habas, Alba Hernandez, Oscar Herrero, Marina Isidori, Awadhesh N. N. Jha, Siegfried Knasmueller, Ingeborg M. M. Kooter, Gudrun Koppen, Marcin Kruszewski, Carina Ladeira, Blanca Laffon, Marcelo Larramendy, Ludovic Le Hegarat, Angelique Lewies, Anna Lewinska, Guillermo E. E. Liwszyc, Adela Lopez de Cerain, Mugimane Manjanatha, Ricard Marcos, Mirta Milic, Vanessa Moraes de Andrade, Massimo Moretti, Damian Muruzabal, Matjaz Novak, Rui Oliveira, Ann-Karin Olsen, Norah Owiti, Mario Pacheco, Alok K. K. Pandey, Stefan Pfuhler, Bertrand Pourrut, Kerstin Reisinger, Emilio Rojas, Elise Runden-Pran, Julen Sanz-Serrano, Sergey Shaposhnikov, Ville Sipinen, Karen Smeets, Helga Stopper, Joao Paulo Teixeira, Vanessa Valdiglesias, Mahara Valverde, Frederique van Acker, Frederik-Jan van Schooten, Marie Vasquez, Johannes F. F. Wentzel, Maciej Wnuk, Annelies Wouters, Bojana Zegura, Tomas Zikmund, Sabine A. S. Langie, Amaya Azqueta
Summary: The comet assay is a versatile method for detecting various types of DNA damage in individual eukaryotic cells. It has been widely used in research, industry, and regulatory agencies, and has been adopted as an in vivo test for genotoxicity. The present document provides consensus protocols for applying the comet assay to different cell types, species, and types of DNA damage.
Article
Environmental Sciences
Rafael Valencia-Quintana, Mirta Milic, Stefano Bonassi, Maria Antonieta Ochoa-Ocana, Victoria Campos-Pena, Maria Guadalupe Tenorio-Arvide, Guillermo Alejandro Perez-Flores, Juana Sanchez-Alarcon
Summary: This study evaluated the effect of pesticides on genetic damage to agricultural workers in Los Reyes, Michoacan, Mexico. The results showed that pesticide use poses a risk of genetic damage to agricultural workers, but measures can be taken to reduce this risk.
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Julen Sanz-Serrano, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo, Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: Measurement of DNA migration in the comet assay can be done through image analysis or visual scoring, with the latter accounting for a significant portion of the published results. This study examines the variability in visual scoring of comets among different investigators and suggests the use of three reference sets for scoring. The results show both inter-investigator and intra-investigator variation in scoring, indicating a need for further standardization, but also demonstrate that visual scoring remains a reliable method for analyzing DNA migration in comets.
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo, Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: Accumulation of DNA strand breaks during cryopreservation is not a concern for mononuclear blood cell lines, and there is inter-laboratory variation in the results.
Article
Genetics & Heredity
Peter Moller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A. S. Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewodzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, Joao Paulo Teixeira, Mirko Marino, Cristian Del Bo', Patrizia Riso, Congying Zheng, Sergey Shaposhnikov, Andrew Collins
Summary: The study finds that potassium bromate is a suitable positive control for the Fpg-modified comet assay, which is widely used to measure oxidatively generated damage to DNA. Although all laboratories obtained positive concentration-response relationships, there was variation in the levels of Fpg-sensitive sites between different laboratories. However, the consistency between the measurements of %Tail DNA values and visual scores of comet images done in the central laboratory suggests a potential source of inter-laboratory variation.
Article
Nanoscience & Nanotechnology
Fatima Brandao, Carla Costa, Maria Joao Bessa, Vanessa Valdiglesias, Bryan Hellack, Andrea Haase, Sonia Fraga, Joao Paulo Teixeira
Summary: This study assessed the cytotoxicity and genotoxicity of different-sized and surface-modified amorphous silica nanomaterials on rat alveolar epithelial cells. The results showed that variants with larger surface areas had higher cytotoxic potential, SiO2_40 induced significant DNA damage, and all tested variants increased gamma-H2AX levels. This study supports the use of multiparametric approaches to improve understanding of nanomaterial mechanisms and hazard prediction.
Article
Environmental Sciences
Mahara Valverde, Adriana Granados, Mirta Milic, Marcello Ceppi, Leticia Sollano, Stefano Bonassi, Emilio Rojas
Summary: DNA damage in newborn children's cord blood cells from Mexico City is correlated with the DNA damage in their mothers, as well as the environmental exposure and complications during pregnancy. Mothers living in the southern part of the city have double DNA damage compared to those living in the northern part. This study highlights the importance of antioxidants during pregnancy and the role of the placental barrier in protecting newborns from DNA-damaging effects of pollution.
Article
Biochemistry & Molecular Biology
Vanessa Valdiglesias, Anabel Alba-Gonzalez, Natalia Fernandez-Bertolez, Assia Touzani, Lucia Ramos-Pan, Ana Teresa Reis, Jorge Moreda-Pineiro, Julian Yanez, Blanca Laffon, Monica Folgueira
Summary: The aim of this study was to investigate the effects of zinc oxide nanoparticles (ZnO NPs) on human glial cells and zebrafish embryo development, as well as to explore the role of released Zn2+ ions in these effects. The results showed that exposure to ZnO NPs caused morphological alterations and a significant decrease in cell viability. Zebrafish embryos also exhibited a decrease in hatching rate. The study further found that these effects were partly attributed to the released Zn2+ ions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Piero Barbanti, Gabriella Egeo, Cinzia Aurilia, Paola Torelli, Cinzia Finocchi, Florindo d'Onofrio, Luigi d'Onofrio, Renata Rao, Stefano Messina, Laura Di Clemente, Angelo Ranieri, Massimo Autunno, Giuliano Sette, Bruno Colombo, Antonio Carnevale, Marco Aguggia, Miriam Tasillo, Francesco Zoroddu, Fabio Frediani, Massimo Filippi, Carlo Tomino, Stefania Proietti, Stefano Bonassi
Summary: This study confirms the long-term efficacy, safety, and tolerability of fremanezumab in real-life patients with high-frequency episodic migraine and chronic migraine. The drug significantly reduced the frequency of migraines and headaches during the 24-week treatment period, with minimal side effects.
JOURNAL OF HEADACHE AND PAIN
(2023)
Review
Biotechnology & Applied Microbiology
Francisco Carlos da Silva Junior, Leticya Pinto de Araujo, Joao Paulo de Mendonca Freitas, Nilmara de Oliveira Alves, Stefano Bonassi, Silvia Regina Batistuzzo de Medeiros
Summary: This study conducted a systematic review and meta-analysis on the association between ambient particulate matter (PM) exposure and micronucleus (MN) frequency. The results showed a significant association between PM exposure and MN frequency, with buccal cells and low economic status as potential effect modifiers. This research is important for understanding the impact of environmental pollution on human health.
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2023)
Article
Nutrition & Dietetics
Marco Crocco, Angela Calvi, Francesca Canzoneri, Federica Malerba, Noemi Zampatti, Andrea Chiaro, Serena Arrigo, Paolo Gandullia, Stefania Proietti, Stefano Bonassi
Summary: This study assessed the impact of the COVID-19 pandemic on the number of new-onset celiac disease cases, and found a 14.5% decrease in cases during the pandemic compared to previous years. This is the first study to evaluate the effect of COVID-19 on celiac disease diagnosis in children.
Article
Geriatrics & Gerontology
Hira Mohyuddin, Blanca Laffon, Joao P. Teixeira, Solange Costa, Armanda Teixeira-Gomes, Eduardo Pasaro, Niel Constantine, Aline Dagdag, Heidi K. Ortmeyer, Boris Tizenberg, Liubov Afram, Poyu Yen, Christopher Marano, Christopher A. Lowry, Andrew J. Hoisington, Jill A. Rachbeisel, Vanessa Valdiglesias, Carlota Lema-Arranz, Natalia Fernandez-Bertolez, Ana Maseda, Jose C. Millan-Calenti, Elizabeth J. Kovacs, Johanna M. Gostner, Dietmar Fuchs, Lisa A. Brenner, Laura Lorenzo-Lopez, Teodor T. Postolache
Summary: This study revealed an association between Toxoplasma gondii and frailty, but further research is needed for validation. Certain biomarkers related to inflammaging were associated with both Toxoplasma gondii and frailty, but they did not fully mediate the association between the two.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)