Redesign of enzyme for improving catalytic activity and enantioselectivity toward poor substrates: manipulation of the transition state

An Efficient Dynamic Kinetic Resolution of N-Heterocyclic 1,2-Amino Alcohols

(2011) Richard Lihammar et al.   ADVANCED SYNTHESIS & CATALYSIS

Ionic-Surfactant-Coated Burkholderia cepacia Lipase as a Highly Active and Enantioselective Catalyst for the Dynamic Kinetic Resolution of Secondary Alcohols

(2011) Hyunjin Kim et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

(2011) Yusuke Miyauchi et al.   CHEMICAL COMMUNICATIONS

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

(2011) Sabrina Kille et al.   Nature Chemistry

A Molecular Balance for Measuring Aliphatic CH−π Interactions

(2011) William R. Carroll et al.   ORGANIC LETTERS

Recent developments in dynamic kinetic resolution

(2011) Hélène Pellissier   TETRAHEDRON

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rationally engineered double substituted variants of Yarrowia lipolytica lipase with enhanced activity coupled with highly inverted enantioselectivity towards 2-bromo phenyl acetic acid esters

(2010) Emmanuelle Cambon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mutant Lipase-Catalyzed Kinetic Resolution of Bulky Phenyl Alkylsec-Alcohols: A Thermodynamic Analysis of Enantioselectivity

(2010) Michaela Vallin et al.   CHEMBIOCHEM

Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site-Saturated Libraries

(2010) Martyn E. Hulley et al.   CHEMBIOCHEM

Rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates

(2010) Tadashi Ema et al.   CHEMICAL COMMUNICATIONS

Mutations in the stereospecificity pocket and at the entrance of the active site of Candida antarctica lipase B enhancing enzyme enantioselectivity

(2010) Z. Marton et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Chemoenzymatic Synthesis of Rivastigmine via Dynamic Kinetic Resolution as a Key Step

(2010) Kiwon Han et al.   JOURNAL OF ORGANIC CHEMISTRY

Manipulating the Stereoselectivity of Limonene Epoxide Hydrolase by Directed Evolution Based on Iterative Saturation Mutagenesis

(2010) Huabao Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Lipase with Broad Substrate Scope for Hydrolysis of α-Substituted Esters

(2010) Karin Engström et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mutated variant of Candida antarcticalipase B in (S)-selective dynamic kinetic resolution of secondary alcohols

(2010) Karin Engström et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

One-Pot Synthesis of Optically Active Allyl Esters via Lipase−Vanadium Combo Catalysis

(2010) Shuji Akai et al.   ORGANIC LETTERS

Origin of Stereoselectivity in the (4 + 3) Cycloadditions of Chiral Alkoxy Siloxyallyl Cations with Furan

(2010) Elizabeth H. Krenske et al.   ORGANIC LETTERS

Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase

(2010) S. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio-, Stereo- and Chemoselectivity

(2009) Alexander Seifert et al.   CHEMBIOCHEM

Improvement ofYarrowia lipolyticaLipase Enantioselectivity by Using Mutagenesis Targeted to the Substrate Binding Site

(2009) F. Bordes et al.   CHEMBIOCHEM

Control of Lipase Enantioselectivity by Engineering the Substrate Binding Site and Access Channel

(2009) Vincent Lafaquière et al.   CHEMBIOCHEM

Increased Enantioselectivity by Engineering Bottleneck Mutants in an Esterase fromPseudomonas fluorescens

(2009) Anna Schließmann et al.   CHEMBIOCHEM

Chemoenzymatic Dynamic Kinetic Resolution of Alcohols and Amines

(2009) Jin Hee Lee et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Dicarbonylchloro(pentabenzylcyclopentadienyl)ruthenium as Racemization Catalyst in the Dynamic Kinetic Resolution of Secondary Alcohols

(2009) Denys Mavrynsky et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Long term continuous chemoenzymatic dynamic kinetic resolution of rac-1-phenylethanol using ionic liquids and supercritical carbon dioxide

(2009) Pedro Lozano et al.   GREEN CHEMISTRY

Enzyme- and Ruthenium-Catalyzed Dynamic Kinetic Asymmetric Transformation of 1,5-Diols. Application to the Synthesis of (+)-Solenopsin A

(2009) Karin Leijondahl et al.   JOURNAL OF ORGANIC CHEMISTRY

Reversed Enantioselectivity of Diisopropyl Fluorophosphatase against Organophosphorus Nerve Agents by Rational Design

(2009) Marco Melzer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

How a Single-Point Mutation in Horseradish Peroxidase Markedly Enhances Enantioselectivity

(2009) Eugene Antipov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enthalpic Nature of the CH/π Interaction Involved in the Recognition of Carbohydrates by Aromatic Compounds, Confirmed by a Novel Interplay of NMR, Calorimetry, and Theoretical Calculations

(2009) Karla Ramírez-Gualito et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants

(2009) Santosh Kumar Padhi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Enantiocomplementary Enzymes: Classification, Molecular Basis for Their Enantiopreference, and Prospects for Mirror-Image Biotransformations

(2008) Paul F. Mugford et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of Galactose Oxidase: Generation of Enantioselective Secondary Alcohol Oxidases

(2008) Franck Escalettes et al.   CHEMBIOCHEM

Substituent Effect onexoStereoselectivity in the 1,3-Dipolar Cycloaddition Reaction of Tulipalin A with Nitrile Ylides

(2008) Petr Melša et al.   JOURNAL OF ORGANIC CHEMISTRY

Carbohydrate−π Interactions: What Are They Worth?

(2008) Zachary R. Laughrey et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highly Enantioselective Dynamic Kinetic Resolution of 1,2−Diarylethanols by a Lipase−Ruthenium Couple

(2008) Mahn-Joo Kim et al.   ORGANIC LETTERS

Highly Efficient Route for Enantioselective Preparation of Chlorohydrins via Dynamic Kinetic Resolution

(2008) Annika Träff et al.   ORGANIC LETTERS

Racemization catalysts for the dynamic kinetic resolution of alcohols and amines

(2007) Yangsoo Ahn et al.   COORDINATION CHEMISTRY REVIEWS